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P07451

- CAH3_HUMAN

UniProt

P07451 - CAH3_HUMAN

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Protein
Carbonic anhydrase 3
Gene
CA3
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Reversible hydration of carbon dioxide.

Catalytic activityi

H2CO3 = CO2 + H2O.

Cofactori

Zinc.

Enzyme regulationi

Activated by proton donors such as imidazole and the dipeptide histidylhistidine. Inhibited by coumarins and sulfonamide derivatives such as acetazolamide.3 Publications

Kineticsi

  1. KM=52.0 mM for CO21 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi94 – 941Zinc; catalytic
Metal bindingi96 – 961Zinc; catalytic
Metal bindingi119 – 1191Zinc; catalytic
Active sitei127 – 1271 By similarity

GO - Molecular functioni

  1. carbonate dehydratase activity Source: ProtInc
  2. nickel cation binding Source: Ensembl
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. bicarbonate transport Source: Reactome
  2. one-carbon metabolic process Source: InterPro
  3. response to ethanol Source: Ensembl
  4. response to oxidative stress Source: Ensembl
  5. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi4.2.1.1. 2681.
ReactomeiREACT_121123. Reversible hydration of carbon dioxide.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbonic anhydrase 3 (EC:4.2.1.1)
Alternative name(s):
Carbonate dehydratase III
Carbonic anhydrase III
Short name:
CA-III
Gene namesi
Name:CA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:1374. CA3.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi64 – 641K → H: Enhanced proton transfer in catalysis. 1 Publication
Mutagenesisi67 – 671R → H: Enhanced proton transfer in catalysis. 1 Publication
Mutagenesisi197 – 1971F → L: Enhanced activity by at least 10-fold. 1 Publication

Organism-specific databases

PharmGKBiPA25990.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 260259Carbonic anhydrase 3
PRO_0000077426Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei182 – 1821S-glutathionyl cysteine By similarity
Modified residuei187 – 1871S-glutathionyl cysteine By similarity

Post-translational modificationi

S-glutathionylated in hepatocytes under oxidative stress By similarity.

Keywords - PTMi

Acetylation, Glutathionylation

Proteomic databases

PaxDbiP07451.
PRIDEiP07451.

2D gel databases

UCD-2DPAGEP07451.

PTM databases

PhosphoSiteiP07451.

Expressioni

Tissue specificityi

Muscle specific.

Developmental stagei

At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.1 Publication

Gene expression databases

ArrayExpressiP07451.
BgeeiP07451.
CleanExiHS_CA3.
GenevestigatoriP07451.

Organism-specific databases

HPAiCAB004967.
HPA021775.
HPA026700.

Interactioni

Protein-protein interaction databases

BioGridi107216. 1 interaction.
STRINGi9606.ENSP00000285381.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni9 – 113
Turni13 – 153
Helixi16 – 183
Helixi21 – 244
Beta strandi25 – 273
Helixi35 – 373
Beta strandi47 – 504
Helixi53 – 553
Beta strandi56 – 616
Beta strandi66 – 705
Beta strandi73 – 753
Beta strandi77 – 815
Beta strandi88 – 9710
Beta strandi106 – 1094
Beta strandi115 – 1239
Helixi125 – 1273
Helixi130 – 1334
Beta strandi139 – 15113
Helixi154 – 1607
Helixi161 – 1655
Beta strandi172 – 1743
Helixi180 – 1834
Beta strandi190 – 1956
Beta strandi206 – 2138
Beta strandi215 – 2173
Helixi219 – 2257
Beta strandi229 – 2313
Beta strandi233 – 2364
Beta strandi256 – 2583

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z93X-ray2.10A1-260[»]
1Z97X-ray2.10A1-260[»]
2HFWX-ray2.50A1-259[»]
3UYNX-ray2.60A1-260[»]
3UYQX-ray1.70A1-260[»]
ProteinModelPortaliP07451.
SMRiP07451. Positions 2-260.

Miscellaneous databases

EvolutionaryTraceiP07451.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni64 – 674Involved in proton transfer
Regioni198 – 1992Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3338.
HOGENOMiHOG000112637.
HOVERGENiHBG002837.
InParanoidiP07451.
KOiK01672.
OMAiEAPFTHF.
PhylomeDBiP07451.
TreeFamiTF316425.

Family and domain databases

Gene3Di3.10.200.10. 1 hit.
InterProiIPR001148. Carbonic_anhydrase_a.
IPR023561. Carbonic_anhydrase_a-class.
IPR018338. Carbonic_anhydrase_a-class_CS.
IPR018441. Carbonic_anhydrase_CA3.
[Graphical view]
PANTHERiPTHR18952. PTHR18952. 1 hit.
PTHR18952:SF96. PTHR18952:SF96. 1 hit.
PfamiPF00194. Carb_anhydrase. 1 hit.
[Graphical view]
SMARTiSM01057. Carb_anhydrase. 1 hit.
[Graphical view]
SUPFAMiSSF51069. SSF51069. 1 hit.
PROSITEiPS00162. ALPHA_CA_1. 1 hit.
PS51144. ALPHA_CA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07451-1 [UniParc]FASTAAdd to Basket

« Hide

MAKEWGYASH NGPDHWHELF PNAKGENQSP VELHTKDIRH DPSLQPWSVS    50
YDGGSAKTIL NNGKTCRVVF DDTYDRSMLR GGPLPGPYRL RQFHLHWGSS 100
DDHGSEHTVD GVKYAAELHL VHWNPKYNTF KEALKQRDGI AVIGIFLKIG 150
HENGEFQIFL DALDKIKTKG KEAPFTKFDP SCLFPACRDY WTYQGSFTTP 200
PCEECIVWLL LKEPMTVSSD QMAKLRSLLS SAENEPPVPL VSNWRPPQPI 250
NNRVVRASFK 260
Length:260
Mass (Da):29,557
Last modified:March 20, 2007 - v3
Checksum:iA305334167233FCF
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti31 – 311V → I.3 Publications
Corresponds to variant rs20571 [ dbSNP | Ensembl ].
VAR_016180

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M29458 Genomic DNA. Translation: AAA52293.1.
M29452 Genomic DNA. No translation available.
AK096880 mRNA. Translation: BAG53390.1.
AK313254 mRNA. Translation: BAG36064.1.
CH471068 Genomic DNA. Translation: EAW87133.1.
BC004897 mRNA. Translation: AAH04897.1.
AJ006473 Genomic DNA. Translation: CAA07056.1.
CCDSiCCDS6238.1.
PIRiA26658. CRHU3.
RefSeqiNP_005172.1. NM_005181.3.
UniGeneiHs.82129.

Genome annotation databases

EnsembliENST00000285381; ENSP00000285381; ENSG00000164879.
GeneIDi761.
KEGGihsa:761.
UCSCiuc003ydj.3. human.

Polymorphism databases

DMDMi134047703.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M29458 Genomic DNA. Translation: AAA52293.1 .
M29452 Genomic DNA. No translation available.
AK096880 mRNA. Translation: BAG53390.1 .
AK313254 mRNA. Translation: BAG36064.1 .
CH471068 Genomic DNA. Translation: EAW87133.1 .
BC004897 mRNA. Translation: AAH04897.1 .
AJ006473 Genomic DNA. Translation: CAA07056.1 .
CCDSi CCDS6238.1.
PIRi A26658. CRHU3.
RefSeqi NP_005172.1. NM_005181.3.
UniGenei Hs.82129.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1Z93 X-ray 2.10 A 1-260 [» ]
1Z97 X-ray 2.10 A 1-260 [» ]
2HFW X-ray 2.50 A 1-259 [» ]
3UYN X-ray 2.60 A 1-260 [» ]
3UYQ X-ray 1.70 A 1-260 [» ]
ProteinModelPortali P07451.
SMRi P07451. Positions 2-260.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 107216. 1 interaction.
STRINGi 9606.ENSP00000285381.

Chemistry

BindingDBi P07451.
ChEMBLi CHEMBL2885.

PTM databases

PhosphoSitei P07451.

Polymorphism databases

DMDMi 134047703.

2D gel databases

UCD-2DPAGE P07451.

Proteomic databases

PaxDbi P07451.
PRIDEi P07451.

Protocols and materials databases

DNASUi 761.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000285381 ; ENSP00000285381 ; ENSG00000164879 .
GeneIDi 761.
KEGGi hsa:761.
UCSCi uc003ydj.3. human.

Organism-specific databases

CTDi 761.
GeneCardsi GC08P086285.
H-InvDB HIX0078885.
HGNCi HGNC:1374. CA3.
HPAi CAB004967.
HPA021775.
HPA026700.
MIMi 114750. gene.
neXtProti NX_P07451.
PharmGKBi PA25990.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG3338.
HOGENOMi HOG000112637.
HOVERGENi HBG002837.
InParanoidi P07451.
KOi K01672.
OMAi EAPFTHF.
PhylomeDBi P07451.
TreeFami TF316425.

Enzyme and pathway databases

BRENDAi 4.2.1.1. 2681.
Reactomei REACT_121123. Reversible hydration of carbon dioxide.

Miscellaneous databases

EvolutionaryTracei P07451.
GeneWikii Carbonic_anhydrase_III,_muscle_specific.
GenomeRNAii 761.
NextBioi 3078.
PROi P07451.
SOURCEi Search...

Gene expression databases

ArrayExpressi P07451.
Bgeei P07451.
CleanExi HS_CA3.
Genevestigatori P07451.

Family and domain databases

Gene3Di 3.10.200.10. 1 hit.
InterProi IPR001148. Carbonic_anhydrase_a.
IPR023561. Carbonic_anhydrase_a-class.
IPR018338. Carbonic_anhydrase_a-class_CS.
IPR018441. Carbonic_anhydrase_CA3.
[Graphical view ]
PANTHERi PTHR18952. PTHR18952. 1 hit.
PTHR18952:SF96. PTHR18952:SF96. 1 hit.
Pfami PF00194. Carb_anhydrase. 1 hit.
[Graphical view ]
SMARTi SM01057. Carb_anhydrase. 1 hit.
[Graphical view ]
SUPFAMi SSF51069. SSF51069. 1 hit.
PROSITEi PS00162. ALPHA_CA_1. 1 hit.
PS51144. ALPHA_CA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and derived amino acid sequence of a cDNA encoding human muscle carbonic anhydrase."
    Lloyd J., McMillan S., Hopkinson D., Edwards Y.H.
    Gene 41:233-239(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Nucleotide sequence, tissue-specific expression, and chromosome location of human carbonic anhydrase III: the human CAIII gene is located on the same chromosome as the closely linked CAI and CAII genes."
    Wade R., Gunning P., Eddy R., Shows T., Kedes L.
    Proc. Natl. Acad. Sci. U.S.A. 83:9571-9575(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-31.
    Tissue: Pericardium and Skeletal muscle.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ILE-31.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-31.
    Tissue: Muscle.
  6. "Human muscle carbonic anhydrase: gene structure and DNA methylation patterns in fetal and adult tissues."
    Lloyd J., Brownson C., Tweedie S., Charlton J., Edwards Y.H.
    Genes Dev. 1:594-602(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  7. "Sequence comparisons and functional studies of the proximal promoter of the carbonic anhydrase 3 (CA3) gene."
    Sowden J., Smith H., Morrison K., Edwards Y.
    Gene 214:157-165(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11, DEVELOPMENTAL STAGE.
  8. "A thiabendazole sulfonamide shows potent inhibitory activity against mammalian and nematode alpha-carbonic anhydrases."
    Crocetti L., Maresca A., Temperini C., Hall R.A., Scozzafava A., Muehlschlegel F.A., Supuran C.T.
    Bioorg. Med. Chem. Lett. 19:1371-1375(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  9. "Non-zinc mediated inhibition of carbonic anhydrases: coumarins are a new class of suicide inhibitors."
    Maresca A., Temperini C., Vu H., Pham N.B., Poulsen S.-A., Scozzafava A., Quinn R.J., Supuran C.T.
    J. Am. Chem. Soc. 131:3057-3062(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  10. "Crystal structure of human carbonic anhydrase XIII and its complex with the inhibitor acetazolamide."
    Di Fiore A., Monti S.M., Hilvo M., Parkkila S., Romano V., Scaloni A., Pedone C., Scozzafava A., Supuran C.T., De Simone G.
    Proteins 74:164-175(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
  11. "Human carbonic anhydrase III: structural and kinetic study of catalysis and proton transfer."
    Duda D.M., Tu C., Fisher S.Z., An H., Yoshioka C., Govindasamy L., Laipis P.J., Agbandje-McKenna M., Silverman D.N., McKenna R.
    Biochemistry 44:10046-10053(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 1-260 IN COMPLEX WITH ZINC ION, MUTAGENESIS OF PHE-197, ACTIVATION BY IMIDAZOLE AND HISTIDYLHISTIDINE.
  12. "Structural and kinetic analysis of proton shuttle residues in the active site of human carbonic anhydrase III."
    Elder I., Fisher Z., Laipis P.J., Tu C., McKenna R., Silverman D.N.
    Proteins 68:337-343(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 1-260 IN COMPLEX WITH ZINC ION, MUTAGENESIS OF LYS-64 AND ARG-67.

Entry informationi

Entry nameiCAH3_HUMAN
AccessioniPrimary (citable) accession number: P07451
Secondary accession number(s): B2R867, B3KUC8, O60842
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: March 20, 2007
Last modified: September 3, 2014
This is version 155 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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