Reviewed, UniProtKB/Swiss-Prot P07451 (CAH3_HUMAN)

Names and origin

Protein names

Recommended name:
    Carbonic anhydrase 3
    EC=4.2.1.1
Alternative name(s):
    Carbonic anhydrase III
      Short name=CA-III
    Carbonate dehydratase III

Gene names

Name:

CA3

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reversible hydration of carbon dioxide.
Catalytic activity	H2CO3 = CO2 + H2O.
Cofactor	Zinc By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Muscle specific.
Developmental stage	At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord. Ref.7
Sequence similarities	Belongs to the alpha-carbonic anhydrase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbonate dehydratase activity Ref.1 Traceable author statement. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 260

259

Carbonic anhydrase 3

PRO_0000077426

Sites

Metal binding

94

1

Zinc; catalytic

Metal binding

96

1

Zinc; catalytic

Metal binding

119

1

Zinc; catalytic

Natural variations

Natural variant

31

1

V → I: dbSNP rs20571. Ref.3 Ref.4 Ref.5

VAR_016180

Secondary structure

1

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260

Helix Strand Turn

Details...

Beta strand

7 – 12

6

Helix

13 – 16

4

Turn

17 – 19

3

Helix

21 – 24

4

Helix

35 – 37

3

Beta strand

47 – 50

4

Helix

53 – 55

3

Beta strand

56 – 61

6

Beta strand

66 – 70

5

Beta strand

73 – 81

9

Beta strand

88 – 97

10

Beta strand

106 – 109

4

Beta strand

115 – 123

9

Helix

125 – 127

3

Helix

130 – 133

4

Beta strand

139 – 151

13

Helix

154 – 162

9

Helix

163 – 166

4

Beta strand

171 – 174

4

Helix

180 – 183

4

Beta strand

190 – 195

6

Beta strand

206 – 213

8

Beta strand

215 – 217

3

Helix

219 – 225

7

Beta strand

229 – 231

3

Beta strand

233 – 235

3

Beta strand

256 – 259

4

Sequences

	Sequence		Length	Mass (Da)
	P07451-1 [UniParc]. Last modified March 20, 2007. Version 3. Checksum: A305334167233FCF	FASTA	260	29,557
	10 20 30 40 50 60 MAKEWGYASH NGPDHWHELF PNAKGENQSP VELHTKDIRH DPSLQPWSVS YDGGSAKTIL 70 80 90 100 110 120 NNGKTCRVVF DDTYDRSMLR GGPLPGPYRL RQFHLHWGSS DDHGSEHTVD GVKYAAELHL 130 140 150 160 170 180 VHWNPKYNTF KEALKQRDGI AVIGIFLKIG HENGEFQIFL DALDKIKTKG KEAPFTKFDP 190 200 210 220 230 240 SCLFPACRDY WTYQGSFTTP PCEECIVWLL LKEPMTVSSD QMAKLRSLLS SAENEPPVPL 250 260 VSNWRPPQPI NNRVVRASFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and derived amino acid sequence of a cDNA encoding human muscle carbonic anhydrase." Lloyd J., McMillan S., Hopkinson D., Edwards Y.H. Gene 41:233-239(1986) [PubMed: 3086182] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Nucleotide sequence, tissue-specific expression, and chromosome location of human carbonic anhydrase III: the human CAIII gene is located on the same chromosome as the closely linked CAI and CAII genes." Wade R., Gunning P., Eddy R., Shows T., Kedes L. Proc. Natl. Acad. Sci. U.S.A. 83:9571-9575(1986) [PubMed: 3099285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-31. Tissue: Pericardium and Skeletal muscle.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ILE-31.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-31. Tissue: Muscle.
[6]	"Human muscle carbonic anhydrase: gene structure and DNA methylation patterns in fetal and adult tissues." Lloyd J., Brownson C., Tweedie S., Charlton J., Edwards Y.H. Genes Dev. 1:594-602(1987) [PubMed: 2824285] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]	"Sequence comparisons and functional studies of the proximal promoter of the carbonic anhydrase 3 (CA3) gene." Sowden J., Smith H., Morrison K., Edwards Y. Gene 214:157-165(1998) [PubMed: 9651514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M29458 Genomic DNA. Translation: AAA52293.1.
M29452 Genomic DNA. No translation available.
AK096880 mRNA. Translation: BAG53390.1.
AK313254 mRNA. Translation: BAG36064.1.
CH471068 Genomic DNA. Translation: EAW87133.1.
BC004897 mRNA. Translation: AAH04897.1.
AJ006473 Genomic DNA. Translation: CAA07056.1.

IPI

IPI00216983.

PIR

CRHU3. A26658.

RefSeq

NP_005172.1.

UniGene

Hs.82129

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Z93	X-ray	2.10	A	1-260	[»]
1Z97	X-ray	2.10	A	1-260	[»]
2HFW	X-ray	2.50	A	1-260	[»]
2HFX	X-ray	1.70	A	1-260	[»]
2HFY	X-ray	2.60	A	1-260	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P07451.

2-D gel databases

HSC-2DPAGE

P07451.

Genome annotation databases

Ensembl

ENST00000285381; ENSP00000285381; ENSG00000164879; Homo sapiens. [Genome view]

GeneID

761.

KEGG

hsa:761.

UCSC

uc003ydj.1. human.

Organism-specific databases

CTD

761.

GeneCards

GC08P086537.

H-InvDB

HIX0078885.

HGNC

HGNC:1374. CA3.

HPA

CAB004967.
HPA021775.
HPA026700.

MIM

114750. gene.

PharmGKB

PA24378.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG14430.

HOGENOM

HBG717384.

HOVERGEN

P07451.

InParanoid

P07451.

OMA

LQPWSVS.

OrthoDB

EOG90KBJT.

PhylomeDB

P07451.

Enzyme and pathway databases

BRENDA

4.2.1.1. 247.

Gene expression databases

ArrayExpress

P07451.

Bgee

P07451.

CleanEx

HS_CA3.

Genevestigator

P07451.

GermOnline

ENSG00000164879. Homo sapiens.

Family and domain databases

InterPro

IPR001148. Carbonic_anhydrase_a-class_cat.
IPR018338. Carbonic_anhydrase_a-class_CS.
IPR018441. Carbonic_anhydrase_CA3.
[Graphical view]

Gene3D

G3DSA:3.10.200.10. Euk_COanhd. 1 hit.

PANTHER

PTHR18952:SF29. Carbonic_anhydrase_CA3. 1 hit.
PTHR18952. Euk_COanhd. 1 hit.

Pfam

PF00194. Carb_anhydrase. 1 hit.
[Graphical view]

PROSITE

PS00162. ALPHA_CA_1. 1 hit.
PS51144. ALPHA_CA_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

3078.

SOURCE

Search...

Entry information

Entry name

CAH3_HUMAN

Accession

Primary (citable) accession number: P07451
Secondary accession number(s): B2R867, B3KUC8, O60842

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	March 20, 2007
Last modified:	January 19, 2010
This is version 118 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.