P07450 (CAH3_HORSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbonic anhydrase 3
EC=4.2.1.1

Alternative name(s):
Carbonate dehydratase III
Carbonic anhydrase III
Short name=CA-III

Gene names

Name:

CA3

Organism

Equus caballus (Horse) [Complete proteome]

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reversible hydration of carbon dioxide.
Catalytic activity	H2CO3 = CO2 + H2O.
Cofactor	Zinc.
Enzyme regulation	Inhibited by acetazolamide By similarity.
Subcellular location	Cytoplasm.
Post-translational modification	S-glutathionylated in hepatocytes under oxidative stress By similarity.
Sequence similarities	Belongs to the alpha-carbonic anhydrase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Lyase
PTM	Acetylation Glutathionylation
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbonate dehydratase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 260	259	Carbonic anhydrase 3		PRO_0000077425
Regions
	Region	64 – 67	4	Involved in proton transfer By similarity
	Region	198 – 199	2	Substrate binding By similarity
Sites
	Active site	127	1	By similarity
	Metal binding	94	1	Zinc; catalytic
	Metal binding	96	1	Zinc; catalytic
	Metal binding	119	1	Zinc; catalytic
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.1
	Modified residue	182	1	S-glutathionyl cysteine By similarity
	Modified residue	187	1	S-glutathionyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07450 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 0BDECDE7C88FED3B	FASTA	260	29,511
	10 20 30 40 50 60 MAKEWGYADH NGPDHWHEFY PIAKGDNQSP IELHTKDINH DPSLKAWTAS YDPGSAKTIL 70 80 90 100 110 120 NNGRTCRVVF DDTYDRSMLR GGPLTAPYRL RQFHLHWGSS DDHGSEHTVD GVKYAAELHL 130 140 150 160 170 180 VHWNPKYNTY GGALKQPDGI AVVGVFLKIG REKGEFQLFL DALDKIKTKG KEAPFTNFDP 190 200 210 220 230 240 SCLFPTCRDY WTYRGSFTTP PCEECIVWLL LKEPITVSSD QVAKLRSLFS SAENEPPVPL 250 260 VRNWRPPQPL KGRVVRASFK

References

[1]	"The sequence of equine muscle carbonic anhydrase." Wendorff K.M., Nishita T., Jabusch J.R., Deutsch H.F. J. Biol. Chem. 260:6129-6132(1985) [PubMed: 3922970] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-260, ACETYLATION AT ALA-2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	A22612.
RefSeq	NP_001157426.1. NM_001163954.1.
UniGene	Eca.16919.
3D structure databases
ProteinModelPortal	P07450.
SMR	P07450. Positions 2-260.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSECAT00000008659; ENSECAP00000006508; ENSECAG00000008422.
GeneID	100050125.
KEGG	ecb:100050125.
Organism-specific databases
CTD	761.
Phylogenomic databases
GeneTree	ENSGT00560000076828.
HOVERGEN	HBG002837.
InParanoid	P07450.
OMA	NGRRIQY.
OrthoDB	EOG4001JV.
Family and domain databases
InterPro	IPR001148. a_carbonic_anhydrase. IPR023561. Carbonic_anhydrase_a-class. IPR018338. Carbonic_anhydrase_a-class_CS. IPR018441. Carbonic_anhydrase_CA3. [Graphical view]
Gene3D	G3DSA:3.10.200.10. Euk_COanhd. 1 hit.
KO	K01672.
PANTHER	PTHR18952:SF29. Carbonic_anhydrase_CA3. 1 hit. PTHR18952. Euk_COanhd. 1 hit.
Pfam	PF00194. Carb_anhydrase. 1 hit. [Graphical view]
SMART	SM01057. Carb_anhydrase. 1 hit. [Graphical view]
SUPFAM	SSF51069. Euk_COanhd. 1 hit.
PROSITE	PS00162. ALPHA_CA_1. 1 hit. PS51144. ALPHA_CA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAH3_HORSE

Accession

Primary (citable) accession number: P07450

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program