Reviewed, UniProtKB/Swiss-Prot P07392 (RPO1_VACCW)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase 147 kDa polypeptide
    EC=2.7.7.6

Gene names

Name:	RPO147
ORF Names:	J6R

Organism

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))

Taxonomic identifier

10254 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	1287 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	This enzyme consists of at least eight subunits.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1287	1287	DNA-directed RNA polymerase 147 kDa polypeptide		PRO_0000073920
Experimental info
	Sequence conflict	62	1	H → Q in CAA26020. Ref.2
	Sequence conflict	83	1	I → T in CAA26020. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P07392-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 570BB7DA1D4EEB76	FASTA	1,287	146,949
	10 20 30 40 50 60 MAVISKVTYS LYDQKEINAT DIIISHVKND DDIGTVKDGR LGAMDGALCK TCGKTELECF 70 80 90 100 110 120 GHWGKVSIYK THIVKPEFIS EIIRLLNHIC IHCGLLRSRE PYSDDINLKE LSGHALRRLK 130 140 150 160 170 180 DKILSKKKSC WNSECMQPYQ KITFSKKKVC FVNKLDDINV PNSLIYQKLI SIHEKFWPLL 190 200 210 220 230 240 EIHQYPANLF YTDYFPIPPL IIRPAISFWI DSIPKETNEL TYLLGMIVKN CNLNADEQVI 250 260 270 280 290 300 QKAVIEYDDI KIISNNTSSI NLSYITSGKN NMIRSYIVAR RKDQTARSVI GPSTSITVNE 310 320 330 340 350 360 VGMPAYIRNT LTEKIFVNAF TVDKVKQLLA SNQVKFYFNK RLNQLTRIRQ GKFIKNKIHL 370 380 390 400 410 420 LPGDWVEVAV QEYTSIIFGR QPSLHRYNVI ASSIRATEGD TIKISPGIAN SQNADFDGDE 430 440 450 460 470 480 EWMILEQNPK AVIEQSILMY PTTLLKHDIH GAPVYGSIQD EIVAAYSLFR IQDLCLDEVL 490 500 510 520 530 540 NILGKYGREF DPKGKCKFSG KDIYTYLIGE KINYPGLLKD GEIIANDVDS NFVVAMRHLS 550 560 570 580 590 600 LAGLLSDHKS NVEGINFIIK SSYVFKRYLS IYGFGVTFKD LRPNSTFTNK LEAINVEKIE 610 620 630 640 650 660 LIKEAYAKYL NDVRDGKIVP LSKALEADYV ESMLSNLTNL NIREIEEHMR QTLIDDPDNN 670 680 690 700 710 720 LLKMAKAGYK VNPTELMYIL GTYGQQRIDG EPAETRVLGR VLPYYLPDSK DPEGRGYILN 730 740 750 760 770 780 SLTKGLTGSQ YYFSMLVARS QSTDIVCETS RTGTLARKII KKMEDMVVDG YGQVVIGNTL 790 800 810 820 830 840 IKYAANYTKI LGSVCKPVDL IYPDESMTWY LEISALWNKI KQGFVYSQKQ KLAKKTLAPF 850 860 870 880 890 900 NFLVFVKPTT EDNAIKVKDL YDMIHNVIDD VREKYFFTVS NIDFMEYIFL THLNPSRIRI 910 920 930 940 950 960 TKETAITIFE KFYEKLNYTL GGGTPIGIIS AQVLSEKFTQ QALSSFHTTE KSGAVKQKLG 970 980 990 1000 1010 1020 FNEFNNLTNL SKNKTEIITL VSDDISKLQS VKINFEFVCL GELNPDHSLF EKKQDRYVVD 1030 1040 1050 1060 1070 1080 IIVNKLYIKR AEITELVVEY MIERFISFSV IVKEWGMETF IEDEDNIRFT VYLNFVEPEE 1090 1100 1110 1120 1130 1140 LNLSKFMMVL PGAANKGKIS KFKIPISDYT GYDCFNQTKK LNKMTVELMN LKELGSFDLE 1150 1160 1170 1180 1190 1200 NVNVYPGVWN TYDIFGIEAA REYLCEAMLN TYGEGFDYLY QPCDLLASLL CASYEPESVN 1210 1220 1230 1240 1250 1260 KFKFGAASTL KRATFGDNKA LLNAALHKKS EPINDNSSCH FFSKVPNIGT GYYKYFIDLG 1270 1280 LLMRMERKLS DKISSQKIKE MEETEDF

References

[1]	"Homology between RNA polymerases of poxviruses, prokaryotes, and eukaryotes: nucleotide sequence and transcriptional analysis of vaccinia virus genes encoding 147-kDa and 22-kDa subunits." Broyles S.S., Moss B. Proc. Natl. Acad. Sci. U.S.A. 83:3141-3145(1986) [PubMed: 3517852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of a cluster of early and late genes in a conserved segment of the vaccinia virus genome." Plucienniczak A., Schroeder E., Zettlmeissl G., Streeck R.E. Nucleic Acids Res. 13:985-998(1985) [PubMed: 2987815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-111.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M13209 Genomic DNA. Translation: AAB59835.1. X01978 Genomic DNA. Translation: CAA26020.1.
PIR	RNVZ47. B25734.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. [Graphical view]
Gene3D	G3DSA:2.40.40.30. RNA_pol_A. 1 hit.
Pfam	PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPO1_VACCW

Accession

Primary (citable) accession number: P07392
Secondary accession number(s): P07619

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)