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Protein

Serine proteinase inhibitor 2

Gene

CRMA

Organism
Cowpox virus (strain Brighton Red) (CPV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specific and potent inhibitor of the interleukin-1-beta converting enzyme (ICE) thereby suppressing an interleukin-1 beta response to infection. The inhibition of ICE by crmA is an example of a "cross-class" interaction, in which a serpin inhibits a non-serine proteinase. Also inhibits granzyme B.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei303 – 304Reactive bondBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Keywords - Biological processi

Host-virus interaction, Inhibition of host caspases by virus, Modulation of host cell apoptosis by virus

Enzyme and pathway databases

ReactomeiR-HSA-5218900. CASP8 activity is inhibited.
R-HSA-5357905. Regulation of TNFR1 signaling.

Protein family/group databases

MEROPSiI04.028.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine proteinase inhibitor 2
Short name:
Serp-2
Short name:
Serpin-2
Alternative name(s):
Cytokine response modifier protein A
Hemorrhage-inducing 38 kDa protein
ICE inhibitor
Gene namesi
Name:CRMA
Synonyms:SPI-2
Ordered Locus Names:CPXV207
OrganismiCowpox virus (strain Brighton Red) (CPV)
Taxonomic identifieri265872 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
Homo sapiens (Human) [TaxID: 9606]
Loxodonta africana (African elephant) [TaxID: 9785]
Microtus agrestis (Short-tailed field vole) [TaxID: 29092]
Mus musculus (Mouse) [TaxID: 10090]
Myodes glareolus (Bank vole) (Clethrionomys glareolus) [TaxID: 447135]

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000941461 – 341Serine proteinase inhibitor 2Add BLAST341

Interactioni

Protein-protein interaction databases

MINTiMINT-139702.

Structurei

Secondary structure

1341
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 10Combined sources9
Turni11 – 13Combined sources3
Beta strandi16 – 18Combined sources3
Helixi20 – 33Combined sources14
Helixi36 – 43Combined sources8
Turni48 – 50Combined sources3
Beta strandi51 – 53Combined sources3
Beta strandi58 – 68Combined sources11
Helixi75 – 81Combined sources7
Helixi82 – 84Combined sources3
Beta strandi85 – 88Combined sources4
Beta strandi90 – 92Combined sources3
Helixi94 – 106Combined sources13
Turni107 – 109Combined sources3
Beta strandi125 – 139Combined sources15
Helixi143 – 145Combined sources3
Beta strandi147 – 154Combined sources8
Beta strandi157 – 166Combined sources10
Beta strandi170 – 176Combined sources7
Beta strandi183 – 203Combined sources21
Helixi207 – 211Combined sources5
Helixi216 – 224Combined sources9
Beta strandi227 – 230Combined sources4
Beta strandi232 – 236Combined sources5
Beta strandi238 – 245Combined sources8
Helixi246 – 252Combined sources7
Beta strandi258 – 260Combined sources3
Turni266 – 268Combined sources3
Beta strandi269 – 271Combined sources3
Beta strandi278 – 286Combined sources9
Beta strandi288 – 299Combined sources12
Beta strandi311 – 313Combined sources3
Beta strandi318 – 324Combined sources7
Beta strandi329 – 335Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C8OX-ray2.90A1-300[»]
B301-341[»]
1F0CX-ray2.26A1-305[»]
B306-341[»]
1M93X-ray1.65A1-55[»]
B56-300[»]
C301-341[»]
ProteinModelPortaliP07385.
SMRiP07385.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07385.

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family. Poxviruses subfamily.Curated

Phylogenomic databases

KOiK13963.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
[Graphical view]
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07385-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIFREIASS MKGENVFISP PSISSVLTIL YYGANGSTAE QLSKYVEKEA
60 70 80 90 100
DKNKDDISFK SMNKVYGRYS AVFKDSFLRK IGDNFQTVDF TDCRTVDAIN
110 120 130 140 150
KCVDIFTEGK INPLLDEPLS PDTCLLAISA VYFKAKWLMP FEKEFTSDYP
160 170 180 190 200
FYVSPTEMVD VSMMSMYGEA FNHASVKESF GNFSIIELPY VGDTSMVVIL
210 220 230 240 250
PDNIDGLESI EQNLTDTNFK KWCDSMDAMF IDVHIPKFKV TGSYNLVDAL
260 270 280 290 300
VKLGLTEVFG STGDYSNMCN SDVSVDAMIH KTYIDVNEEY TEAAAATCAL
310 320 330 340
VADCASTVTN EFCADHPFIY VIRHVDGKIL FVGRYCSPTT N
Length:341
Mass (Da):38,077
Last modified:April 1, 1988 - v1
Checksum:i25AE4E0CB123E172
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14217 Genomic DNA. Translation: AAA42922.1.
AF482758 Genomic DNA. Translation: AAM13646.1.
PIRiA26477. WMVZHI.
RefSeqiNP_619988.1. NC_003663.2.

Genome annotation databases

GeneIDi1486086.
KEGGivg:1486086.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14217 Genomic DNA. Translation: AAA42922.1.
AF482758 Genomic DNA. Translation: AAM13646.1.
PIRiA26477. WMVZHI.
RefSeqiNP_619988.1. NC_003663.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C8OX-ray2.90A1-300[»]
B301-341[»]
1F0CX-ray2.26A1-305[»]
B306-341[»]
1M93X-ray1.65A1-55[»]
B56-300[»]
C301-341[»]
ProteinModelPortaliP07385.
SMRiP07385.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-139702.

Protein family/group databases

MEROPSiI04.028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1486086.
KEGGivg:1486086.

Phylogenomic databases

KOiK13963.

Enzyme and pathway databases

ReactomeiR-HSA-5218900. CASP8 activity is inhibited.
R-HSA-5357905. Regulation of TNFR1 signaling.

Miscellaneous databases

EvolutionaryTraceiP07385.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
[Graphical view]
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPI2_CWPXB
AccessioniPrimary (citable) accession number: P07385
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.