Calpain-1 catalytic subunit - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot P07384 (CAN1_HUMAN)

Last modified July 7, 2009. Version 121. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Calpain-1 catalytic subunit
    EC=3.4.22.52
Alternative name(s):
    Calpain-1 large subunit
    Calcium-activated neutral proteinase 1
      Short name=CANP 1
    Calpain mu-type
    muCANP
    Micromolar-calpain
    Cell proliferation-inducing gene 30 protein

Gene names

Name:	CAPN1
Synonyms:	CANPL1
ORF Names:	PIG30

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	714 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Catalytic activity	Broad endopeptidase specificity.
Cofactor	Binds 3 calcium ions.
Enzyme regulation	Activated by micromolar concentrations of calcium and inhibited by calpastatin.
Subunit structure	Forms a heterodimer with a small (regulatory) subunit (CAPNS1).
Subcellular location	Cytoplasm By similarity. Cell membrane By similarity. Note: Translocates to the plasma membrane upon Ca²⁺ binding By similarity.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 4 EF-hand domains.

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Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	Calcium
Molecular function	Hydrolase Protease Thiol protease
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	positive regulation of cell proliferation Traceable author statement. Source: ProtInc proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: HPA plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-dependent cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
RCAN1	P53805-2	1	EBI-1542113,EBI-1541912

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 714

714

Calpain-1 catalytic subunit

PRO_0000207694

Regions

Domain

55 – 354

300

Calpain catalytic

Domain

541 – 576

EF-hand 1

Domain

585 – 618

EF-hand 2

Domain

615 – 650

EF-hand 3

Domain

680 – 714

EF-hand 4

Calcium binding

598 – 609

Calcium binding

628 – 639

Region

355 – 526

172

Domain III

Region

527 – 542

Linker

Region

543 – 713

171

Domain IV

Sites

Active site

115

By similarity

Active site

272

By similarity

Active site

296

By similarity

Natural variations

Natural variant

103

T → A: dbSNP rs17885718. Ref.4

VAR_021085

Natural variant

433

R → P: dbSNP rs10895991. Ref.4 Ref.5

VAR_021086

Natural variant

492

G → R: dbSNP rs17883283. Ref.4

VAR_021087

Natural variant

676

V → I: dbSNP rs17884773. Ref.4

VAR_021088

Experimental info

Sequence conflict

548

K → N in AAH08751. Ref.5

Secondary structure

714

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P07384-1 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 1CB6D7C56D063498
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FASTA

714

81,890

        10         20         30         40         50         60 
MSEEIITPVY CTGVSAQVQK QRARELGLGR HENAIKYLGQ DYEQLRVRCL QSGTLFRDEA 

        70         80         90        100        110        120 
FPPVPQSLGY KDLGPNSSKT YGIKWKRPTE LLSNPQFIVD GATRTDICQG ALGDCWLLAA 

       130        140        150        160        170        180 
IASLTLNDTL LHRVVPHGQS FQNGYAGIFH FQLWQFGEWV DVVVDDLLPI KDGKLVFVHS 

       190        200        210        220        230        240 
AEGNEFWSAL LEKAYAKVNG SYEALSGGST SEGFEDFTGG VTEWYELRKA PSDLYQIILK 

       250        260        270        280        290        300 
ALERGSLLGC SIDISSVLDM EAITFKKLVK GHAYSVTGAK QVNYRGQVVS LIRMRNPWGE 

       310        320        330        340        350        360 
VEWTGAWSDS SSEWNNVDPY ERDQLRVKME DGEFWMSFRD FMREFTRLEI CNLTPDALKS 

       370        380        390        400        410        420 
RTIRKWNTTL YEGTWRRGST AGGCRNYPAT FWVNPQFKIR LDETDDPDDY GDRESGCSFV 

       430        440        450        460        470        480 
LALMQKHRRR ERRFGRDMET IGFAVYEVPP ELVGQPAVHL KRDFFLANAS RARSEQFINL 

       490        500        510        520        530        540 
REVSTRFRLP PGEYVVVPST FEPNKEGDFV LRFFSEKSAG TVELDDQIQA NLPDEQVLSE 

       550        560        570        580        590        600 
EEIDENFKAL FRQLAGEDME ISVKELRTIL NRIISKHKDL RTKGFSLESC RSMVNLMDRD 

       610        620        630        640        650        660 
GNGKLGLVEF NILWNRIRNY LSIFRKFDLD KSGSMSAYEM RMAIESAGFK LNKKLYELII 

       670        680        690        700        710 
TRYSEPDLAV DFDNFVCCLV RLETMFRFFK TLDTDLDGVV TFDLFKWLQL TMFA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete amino acid sequence of the large subunit of the low-Ca2+-requiring form of human Ca2+-activated neutral protease (muCANP) deduced from its cDNA sequence." Aoki K., Imajoh S., Ohno S., Emori Y., Koike M., Kosaki G., Suzuki K. FEBS Lett. 205:313-317(1986) [PubMed: 3017764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A novel member of the calcium-dependent cysteine protease family." Sorimachi H., Ohmi S., Emori Y., Kawasaki H., Saido T.C., Ohno S., Minami Y., Suzuki K. Biol. Chem. Hoppe-Seyler 371:171-176(1990) [PubMed: 2400579] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Identification of a human cell proliferation inducing gene." Kim J.W. Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	NIEHS SNPs program Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-103; PRO-433; ARG-492 AND ILE-676.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-433. Tissue: Kidney, Pancreas and Placenta.
[6]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Web resources

CaBP

Calpain

Calpains homepage

NIEHS-SNPs

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Cross-references

Sequence databases

X04366 mRNA. Translation: CAA27881.1.
AY550975 mRNA. Translation: AAT52221.1.
AY796340 Genomic DNA. Translation: AAV41878.1.
BC008751 mRNA. Translation: AAH08751.1.
BC017200 mRNA. Translation: AAH17200.1.
BC075862 mRNA. Translation: AAH75862.1.

IPI

IPI00011285.

PIR

CIHUH. A26213.

RefSeq

NP_005177.2.

UniGene

Hs.502842

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZCM	X-ray	2.00	A	33-353	[»]
2ARY	X-ray	2.40	A/B	29-360	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P07384. 3 interactions.

Protein family/group databases

MEROPS

C02.001.

Proteomic databases

PRIDE

P07384.

Genome annotation databases

Ensembl

ENSG00000014216. Homo sapiens. [Contig view]

GeneID

823.

KEGG

hsa:823.

UCSC

uc001odf.1. human.

Organism-specific databases

GeneCards

GC11P064705.

H-InvDB

HIX0009788.

HGNC

HGNC:1476. CAPN1.

HPA

HPA005992.

MIM

114220. gene.

PharmGKB

PA26057.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P07384.

HOVERGEN

P07384.

Enzyme and pathway databases

BRENDA

3.4.22.52. 247.
3.4.22.53. 247.

Pathway_Interaction_DB

ptp1bpathway. Signaling events mediated by PTP1B.

Gene expression databases

ArrayExpress

P07384.

Bgee

P07384.

CleanEx

HS_CAPN1.

GermOnline

ENSG00000014216. Homo sapiens.

Family and domain databases

InterPro

IPR011992. EF-Hand_type.
IPR018247. EF_HAND_1.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca_bd.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 1 hit.

Pfam

PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]

PRINTS

PR00704. CALPAIN.

ProDom

PD000012. EF-hand. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]

PROSITE

PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00640. THIOL_PROTEASE_ASN. False negative.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. False negative.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

3370.

PMAP-CutDB

P07384.

SOURCE

Search...

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Entry information

Entry name

CAN1_HUMAN

Accession

Primary (citable) accession number: P07384
Secondary accession number(s): Q2TTR0, Q6DHV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	July 7, 2009
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Experimental info

Secondary structure

Sequences

References

Web resources

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents