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P07378 (PGKC_TRYBB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase, glycosomal

Short name=Phosphoglycerate kinase C
EC=2.7.2.3
OrganismTrypanosoma brucei brucei
Taxonomic identifier5702 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145

Subunit structure

Monomer.

Subcellular location

Glycosome HAMAP-Rule MF_00145.

Miscellaneous

In T.brucei, three genes code for phosphoglycerate kinase isozymes, which are transported to different cell compartments.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentGlycosome
Peroxisome
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentglycosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 440440Phosphoglycerate kinase, glycosomal HAMAP-Rule MF_00145
PRO_0000145866

Regions

Nucleotide binding375 – 3784ATP HAMAP-Rule MF_00145
Region24 – 263Substrate binding HAMAP-Rule MF_00145
Region62 – 654Substrate binding HAMAP-Rule MF_00145

Sites

Binding site391Substrate
Binding site1351Substrate
Binding site1721Substrate
Binding site2231ATP
Binding site3381ATP
Binding site3451ATP

Natural variations

Natural variant751G → D in allele 4.

Secondary structure

............................................................................... 440
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07378 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: EF95775C216ABDC3

FASTA44047,119
        10         20         30         40         50         60 
MTLNEKKSIN ECDLKGKKVL IRVDFNVPVK NGKITNDYRI RSALPTLKKV LTEGGSCVLM 

        70         80         90        100        110        120 
SHLGRPKGIP MAQAGKIRST GGVPGFQQKA TLKPVAKALS ELLLRPVTFA PDCLNAADVV 

       130        140        150        160        170        180 
SKMSPGDVVL LENVRFYKEE GSKKAKDREA MAKILASYGD VYISDAFGTA HRDSATMTGI 

       190        200        210        220        230        240 
PKILGNGAAG YLMEKEISYF AKVLGNPPRP LVAIVGGAKV SDKIQLLDNM LQRIDYLLIG 

       250        260        270        280        290        300 
GAMAYTFLKA QGYSIGKSKC EESKLEFARS LLKKAEDRKV QVILPIDHVC HTEFKAVDSP 

       310        320        330        340        350        360 
LITEDQNIPE GHMALDIGPK TIEKYVQTIG KCKSAIWNGP MGVFEMVPYS KGTFAIAKAM 

       370        380        390        400        410        420 
GRGTHEHGLM SIIGGGDSAS AAELSGEAKR MSHVSTGGGA SLELLEGKTL PGVTVLDEKS 

       430        440 
AVVSYASAGT GTLSNRWSSL 

« Hide

References

[1]"Evidence for gene conversion between the phosphoglycerate kinase genes of Trypanosoma brucei."
le Blancq S.M., Swinkels B.W., Gibson W.C., Borst P.
J. Mol. Biol. 200:439-447(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELES 2 AND 4).
[2]"Topogenesis of microbody enzymes: a sequence comparison of the genes for the glycosomal (microbody) and cytosolic phosphoglycerate kinases of Trypanosoma brucei."
Osinga K.A., Swinkels B.W., Gibson W.C., Borst P., Veeneman G.H., van Boom J.H., Michels P.A.M., Opperdoes F.R.
EMBO J. 4:3811-3817(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Synergistic effects of substrate-induced conformational changes in phosphoglycerate kinase activation."
Bernstein B.E., Michels P.A.M., Hol W.G.J.
Nature 385:275-278(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
[4]"A bisubstrate analog induces unexpected conformational changes in phosphoglycerate kinase from Trypanosoma brucei."
Bernstein B.E., Williams D.M., Bressi J.C., Kuhn P., Gelb M.H., Blackburn G.M., Hol W.G.J.
J. Mol. Biol. 279:1137-1148(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 5-419 IN COMPLEX WITH ADP AND SUBSTRATE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03370 Genomic DNA. Translation: CAA27069.1.
X05889 Genomic DNA. Translation: CAA29318.1.
X05890 Genomic DNA. Translation: CAA29321.1.
PIRKIUTGG. B25119.
TVUTGB. S02235.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
13PKX-ray2.50A/B/C/D5-417[»]
16PKX-ray1.60A5-417[»]
ProteinModelPortalP07378.
SMRP07378. Positions 5-419.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL1741167.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP07378.
UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR027250. Pgk_euglenozoa.
IPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PIRSF500126. Pgk_euglenozoa. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP07378.

Entry information

Entry namePGKC_TRYBB
AccessionPrimary (citable) accession number: P07378
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: February 19, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways