P07378 (PGKC_TRYBB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Phosphoglycerate kinase, glycosomal Short name=Phosphoglycerate kinase C EC=2.7.2.3
Organism	Trypanosoma brucei brucei
Taxonomic identifier	5702 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma › Trypanozoon › Trypanosoma brucei

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Subunit structure	Monomer.
Subcellular location	Glycosome.
Miscellaneous	In T.brucei, three genes code for phosphoglycerate kinase isozymes, which are transported to different cell compartments.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Glycosome Peroxisome
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	glycosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 440

440

Phosphoglycerate kinase, glycosomal

PRO_0000145866

Regions

Nucleotide binding

375 – 378

4

ATP

Region

24 – 26

3

Substrate binding

Region

62 – 65

4

Substrate binding

Sites

Binding site

39

1

Substrate

Binding site

135

1

Substrate

Binding site

172

1

Substrate

Binding site

223

1

ATP

Binding site

338

1

ATP

Binding site

345

1

ATP

Natural variations

Natural variant

75

1

G → D in allele 4.

Secondary structure

1

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440

Helix Strand Turn

Details...

Helix

9 – 11

3

Beta strand

18 – 23

6

Beta strand

33 – 35

3

Helix

38 – 52

15

Beta strand

56 – 60

5

Helix

71 – 73

3

Helix

74 – 79

6

Helix

88 – 90

3

Helix

93 – 103

11

Beta strand

108 – 111

4

Helix

117 – 121

5

Beta strand

128 – 131

4

Helix

134 – 136

3

Helix

138 – 141

4

Helix

145 – 156

12

Beta strand

160 – 165

6

Helix

167 – 169

3

Turn

175 – 178

4

Helix

179 – 184

6

Helix

191 – 204

14

Beta strand

209 – 215

7

Helix

221 – 223

3

Helix

224 – 230

7

Helix

231 – 233

3

Beta strand

235 – 239

5

Helix

243 – 251

9

Helix

262 – 264

3

Helix

265 – 277

13

Beta strand

281 – 283

3

Beta strand

286 – 295

10

Beta strand

304 – 306

3

Beta strand

313 – 317

5

Helix

319 – 329

11

Beta strand

333 – 339

7

Helix

347 – 349

3

Helix

351 – 367

17

Beta strand

370 – 373

4

Helix

376 – 384

9

Turn

388 – 390

3

Beta strand

391 – 394

4

Helix

398 – 405

8

Helix

411 – 414

4

Sequences

	Sequence		Length	Mass (Da)
	P07378 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: EF95775C216ABDC3	FASTA	440	47,119
	10 20 30 40 50 60 MTLNEKKSIN ECDLKGKKVL IRVDFNVPVK NGKITNDYRI RSALPTLKKV LTEGGSCVLM 70 80 90 100 110 120 SHLGRPKGIP MAQAGKIRST GGVPGFQQKA TLKPVAKALS ELLLRPVTFA PDCLNAADVV 130 140 150 160 170 180 SKMSPGDVVL LENVRFYKEE GSKKAKDREA MAKILASYGD VYISDAFGTA HRDSATMTGI 190 200 210 220 230 240 PKILGNGAAG YLMEKEISYF AKVLGNPPRP LVAIVGGAKV SDKIQLLDNM LQRIDYLLIG 250 260 270 280 290 300 GAMAYTFLKA QGYSIGKSKC EESKLEFARS LLKKAEDRKV QVILPIDHVC HTEFKAVDSP 310 320 330 340 350 360 LITEDQNIPE GHMALDIGPK TIEKYVQTIG KCKSAIWNGP MGVFEMVPYS KGTFAIAKAM 370 380 390 400 410 420 GRGTHEHGLM SIIGGGDSAS AAELSGEAKR MSHVSTGGGA SLELLEGKTL PGVTVLDEKS 430 440 AVVSYASAGT GTLSNRWSSL

References

[1]	"Evidence for gene conversion between the phosphoglycerate kinase genes of Trypanosoma brucei." le Blancq S.M., Swinkels B.W., Gibson W.C., Borst P. J. Mol. Biol. 200:439-447(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELES 2 AND 4).
[2]	"Topogenesis of microbody enzymes: a sequence comparison of the genes for the glycosomal (microbody) and cytosolic phosphoglycerate kinases of Trypanosoma brucei." Osinga K.A., Swinkels B.W., Gibson W.C., Borst P., Veeneman G.H., van Boom J.H., Michels P.A.M., Opperdoes F.R. EMBO J. 4:3811-3817(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Synergistic effects of substrate-induced conformational changes in phosphoglycerate kinase activation." Bernstein B.E., Michels P.A.M., Hol W.G.J. Nature 385:275-278(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
[4]	"A bisubstrate analog induces unexpected conformational changes in phosphoglycerate kinase from Trypanosoma brucei." Bernstein B.E., Williams D.M., Bressi J.C., Kuhn P., Gelb M.H., Blackburn G.M., Hol W.G.J. J. Mol. Biol. 279:1137-1148(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 5-419 IN COMPLEX WITH ADP AND SUBSTRATE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X03370 Genomic DNA. Translation: CAA27069.1.
X05889 Genomic DNA. Translation: CAA29318.1.
X05890 Genomic DNA. Translation: CAA29321.1.

PIR

KIUTGG. B25119.
TVUTGB. S02235.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
13PK	X-ray	2.50	A/B/C/D	5-417	[»]
16PK	X-ray	1.60	A	5-417	[»]

ProteinModelPortal

P07378.

SMR

P07378. Positions 5-419.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

SABIO-RK

P07378.

UniPathway

UPA00109; UER00185.

Family and domain databases

Gene3D

3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.

InterPro

IPR027250. Pgk_euglenozoa.
IPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]

PANTHER

PTHR11406. PTHR11406. 1 hit.

Pfam

PF00162. PGK. 1 hit.
[Graphical view]

PIRSF

PIRSF000724. Pgk. 1 hit.
PIRSF500126. Pgk_euglenozoa. 1 hit.

PRINTS

PR00477. PHGLYCKINASE.

SUPFAM

SSF53748. PGK. 1 hit.

PROSITE

PS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChEMBL

CHEMBL1741167.

EvolutionaryTrace

P07378.

Entry information

Entry name

PGKC_TRYBB

Accession

Primary (citable) accession number: P07378

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)