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P07378

- PGKC_TRYBB

UniProt

P07378 - PGKC_TRYBB

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Protein
Phosphoglycerate kinase, glycosomal
Gene
N/A
Organism
Trypanosoma brucei brucei
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391Substrate
Binding sitei135 – 1351Substrate
Binding sitei172 – 1721Substrate
Binding sitei223 – 2231ATP
Binding sitei338 – 3381ATP
Binding sitei345 – 3451ATP

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi375 – 3784ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphoglycerate kinase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP07378.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase, glycosomal (EC:2.7.2.3)
Short name:
Phosphoglycerate kinase C
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Subcellular locationi

Glycosome UniRule annotation

GO - Cellular componenti

  1. glycosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glycosome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Phosphoglycerate kinase, glycosomalUniRule annotation
PRO_0000145866Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 113
Beta strandi18 – 236
Beta strandi33 – 353
Helixi38 – 5215
Beta strandi56 – 605
Helixi71 – 733
Helixi74 – 796
Helixi88 – 903
Helixi93 – 10311
Beta strandi108 – 1114
Helixi117 – 1215
Beta strandi128 – 1314
Helixi134 – 1363
Helixi138 – 1414
Helixi145 – 15612
Beta strandi160 – 1656
Helixi167 – 1693
Turni175 – 1784
Helixi179 – 1846
Helixi191 – 20414
Beta strandi209 – 2157
Helixi221 – 2233
Helixi224 – 2307
Helixi231 – 2333
Beta strandi235 – 2395
Helixi243 – 2519
Helixi262 – 2643
Helixi265 – 27713
Beta strandi281 – 2833
Beta strandi286 – 29510
Beta strandi304 – 3063
Beta strandi313 – 3175
Helixi319 – 32911
Beta strandi333 – 3397
Helixi347 – 3493
Helixi351 – 36717
Beta strandi370 – 3734
Helixi376 – 3849
Turni388 – 3903
Beta strandi391 – 3944
Helixi398 – 4058
Helixi411 – 4144

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
13PKX-ray2.50A/B/C/D5-417[»]
16PKX-ray1.60A5-417[»]
ProteinModelPortaliP07378.
SMRiP07378. Positions 5-419.

Miscellaneous databases

EvolutionaryTraceiP07378.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 263Substrate bindingUniRule annotation
Regioni62 – 654Substrate bindingUniRule annotation

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR027250. Pgk_euglenozoa.
IPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PIRSF500126. Pgk_euglenozoa. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07378-1 [UniParc]FASTAAdd to Basket

« Hide

MTLNEKKSIN ECDLKGKKVL IRVDFNVPVK NGKITNDYRI RSALPTLKKV    50
LTEGGSCVLM SHLGRPKGIP MAQAGKIRST GGVPGFQQKA TLKPVAKALS 100
ELLLRPVTFA PDCLNAADVV SKMSPGDVVL LENVRFYKEE GSKKAKDREA 150
MAKILASYGD VYISDAFGTA HRDSATMTGI PKILGNGAAG YLMEKEISYF 200
AKVLGNPPRP LVAIVGGAKV SDKIQLLDNM LQRIDYLLIG GAMAYTFLKA 250
QGYSIGKSKC EESKLEFARS LLKKAEDRKV QVILPIDHVC HTEFKAVDSP 300
LITEDQNIPE GHMALDIGPK TIEKYVQTIG KCKSAIWNGP MGVFEMVPYS 350
KGTFAIAKAM GRGTHEHGLM SIIGGGDSAS AAELSGEAKR MSHVSTGGGA 400
SLELLEGKTL PGVTVLDEKS AVVSYASAGT GTLSNRWSSL 440
Length:440
Mass (Da):47,119
Last modified:April 1, 1988 - v1
Checksum:iEF95775C216ABDC3
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti75 – 751G → D in allele 4.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03370 Genomic DNA. Translation: CAA27069.1.
X05889 Genomic DNA. Translation: CAA29318.1.
X05890 Genomic DNA. Translation: CAA29321.1.
PIRiB25119. KIUTGG.
S02235. TVUTGB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03370 Genomic DNA. Translation: CAA27069.1 .
X05889 Genomic DNA. Translation: CAA29318.1 .
X05890 Genomic DNA. Translation: CAA29321.1 .
PIRi B25119. KIUTGG.
S02235. TVUTGB.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
13PK X-ray 2.50 A/B/C/D 5-417 [» ]
16PK X-ray 1.60 A 5-417 [» ]
ProteinModelPortali P07378.
SMRi P07378. Positions 5-419.
ModBasei Search...

Chemistry

ChEMBLi CHEMBL1741167.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00185 .
SABIO-RK P07378.

Miscellaneous databases

EvolutionaryTracei P07378.

Family and domain databases

Gene3Di 3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPi MF_00145. Phosphoglyc_kinase.
InterProi IPR027250. Pgk_euglenozoa.
IPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view ]
PANTHERi PTHR11406. PTHR11406. 1 hit.
Pfami PF00162. PGK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000724. Pgk. 1 hit.
PIRSF500126. Pgk_euglenozoa. 1 hit.
PRINTSi PR00477. PHGLYCKINASE.
SUPFAMi SSF53748. SSF53748. 1 hit.
PROSITEi PS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Evidence for gene conversion between the phosphoglycerate kinase genes of Trypanosoma brucei."
    le Blancq S.M., Swinkels B.W., Gibson W.C., Borst P.
    J. Mol. Biol. 200:439-447(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELES 2 AND 4).
  2. "Topogenesis of microbody enzymes: a sequence comparison of the genes for the glycosomal (microbody) and cytosolic phosphoglycerate kinases of Trypanosoma brucei."
    Osinga K.A., Swinkels B.W., Gibson W.C., Borst P., Veeneman G.H., van Boom J.H., Michels P.A.M., Opperdoes F.R.
    EMBO J. 4:3811-3817(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Synergistic effects of substrate-induced conformational changes in phosphoglycerate kinase activation."
    Bernstein B.E., Michels P.A.M., Hol W.G.J.
    Nature 385:275-278(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
  4. "A bisubstrate analog induces unexpected conformational changes in phosphoglycerate kinase from Trypanosoma brucei."
    Bernstein B.E., Williams D.M., Bressi J.C., Kuhn P., Gelb M.H., Blackburn G.M., Hol W.G.J.
    J. Mol. Biol. 279:1137-1148(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 5-419 IN COMPLEX WITH ADP AND SUBSTRATE.

Entry informationi

Entry nameiPGKC_TRYBB
AccessioniPrimary (citable) accession number: P07378
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: June 11, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

In T.brucei, three genes code for phosphoglycerate kinase isozymes, which are transported to different cell compartments.

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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