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P07378

- PGKC_TRYBB

UniProt

P07378 - PGKC_TRYBB

Protein

Phosphoglycerate kinase, glycosomal

Gene
N/A
Organism
Trypanosoma brucei brucei
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei39 – 391Substrate1 Publication
    Binding sitei135 – 1351Substrate1 Publication
    Binding sitei172 – 1721Substrate1 Publication
    Binding sitei223 – 2231ATP
    Binding sitei338 – 3381ATP
    Binding sitei345 – 3451ATP

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi375 – 3784ATP

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. phosphoglycerate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RKP07378.
    UniPathwayiUPA00109; UER00185.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoglycerate kinase, glycosomal (EC:2.7.2.3)
    Short name:
    Phosphoglycerate kinase C
    OrganismiTrypanosoma brucei brucei
    Taxonomic identifieri5702 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

    Subcellular locationi

    GO - Cellular componenti

    1. glycosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Glycosome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 440440Phosphoglycerate kinase, glycosomalPRO_0000145866Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Structurei

    Secondary structure

    1
    440
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 113
    Beta strandi18 – 236
    Beta strandi33 – 353
    Helixi38 – 5215
    Beta strandi56 – 605
    Helixi71 – 733
    Helixi74 – 796
    Helixi88 – 903
    Helixi93 – 10311
    Beta strandi108 – 1114
    Helixi117 – 1215
    Beta strandi128 – 1314
    Helixi134 – 1363
    Helixi138 – 1414
    Helixi145 – 15612
    Beta strandi160 – 1656
    Helixi167 – 1693
    Turni175 – 1784
    Helixi179 – 1846
    Helixi191 – 20414
    Beta strandi209 – 2157
    Helixi221 – 2233
    Helixi224 – 2307
    Helixi231 – 2333
    Beta strandi235 – 2395
    Helixi243 – 2519
    Helixi262 – 2643
    Helixi265 – 27713
    Beta strandi281 – 2833
    Beta strandi286 – 29510
    Beta strandi304 – 3063
    Beta strandi313 – 3175
    Helixi319 – 32911
    Beta strandi333 – 3397
    Helixi347 – 3493
    Helixi351 – 36717
    Beta strandi370 – 3734
    Helixi376 – 3849
    Turni388 – 3903
    Beta strandi391 – 3944
    Helixi398 – 4058
    Helixi411 – 4144

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    13PKX-ray2.50A/B/C/D5-417[»]
    16PKX-ray1.60A5-417[»]
    ProteinModelPortaliP07378.
    SMRiP07378. Positions 5-419.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07378.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni24 – 263Substrate binding
    Regioni62 – 654Substrate binding

    Sequence similaritiesi

    Belongs to the phosphoglycerate kinase family.Curated

    Family and domain databases

    Gene3Di3.40.50.1260. 1 hit.
    3.40.50.1270. 1 hit.
    HAMAPiMF_00145. Phosphoglyc_kinase.
    InterProiIPR027250. Pgk_euglenozoa.
    IPR001576. Phosphoglycerate_kinase.
    IPR015901. Phosphoglycerate_kinase_C.
    IPR015911. Phosphoglycerate_kinase_CS.
    IPR015824. Phosphoglycerate_kinase_N.
    [Graphical view]
    PANTHERiPTHR11406. PTHR11406. 1 hit.
    PfamiPF00162. PGK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000724. Pgk. 1 hit.
    PIRSF500126. Pgk_euglenozoa. 1 hit.
    PRINTSiPR00477. PHGLYCKINASE.
    SUPFAMiSSF53748. SSF53748. 1 hit.
    PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07378-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLNEKKSIN ECDLKGKKVL IRVDFNVPVK NGKITNDYRI RSALPTLKKV    50
    LTEGGSCVLM SHLGRPKGIP MAQAGKIRST GGVPGFQQKA TLKPVAKALS 100
    ELLLRPVTFA PDCLNAADVV SKMSPGDVVL LENVRFYKEE GSKKAKDREA 150
    MAKILASYGD VYISDAFGTA HRDSATMTGI PKILGNGAAG YLMEKEISYF 200
    AKVLGNPPRP LVAIVGGAKV SDKIQLLDNM LQRIDYLLIG GAMAYTFLKA 250
    QGYSIGKSKC EESKLEFARS LLKKAEDRKV QVILPIDHVC HTEFKAVDSP 300
    LITEDQNIPE GHMALDIGPK TIEKYVQTIG KCKSAIWNGP MGVFEMVPYS 350
    KGTFAIAKAM GRGTHEHGLM SIIGGGDSAS AAELSGEAKR MSHVSTGGGA 400
    SLELLEGKTL PGVTVLDEKS AVVSYASAGT GTLSNRWSSL 440
    Length:440
    Mass (Da):47,119
    Last modified:April 1, 1988 - v1
    Checksum:iEF95775C216ABDC3
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti75 – 751G → D in allele 4.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03370 Genomic DNA. Translation: CAA27069.1.
    X05889 Genomic DNA. Translation: CAA29318.1.
    X05890 Genomic DNA. Translation: CAA29321.1.
    PIRiB25119. KIUTGG.
    S02235. TVUTGB.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03370 Genomic DNA. Translation: CAA27069.1 .
    X05889 Genomic DNA. Translation: CAA29318.1 .
    X05890 Genomic DNA. Translation: CAA29321.1 .
    PIRi B25119. KIUTGG.
    S02235. TVUTGB.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    13PK X-ray 2.50 A/B/C/D 5-417 [» ]
    16PK X-ray 1.60 A 5-417 [» ]
    ProteinModelPortali P07378.
    SMRi P07378. Positions 5-419.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL1741167.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00185 .
    SABIO-RK P07378.

    Miscellaneous databases

    EvolutionaryTracei P07378.

    Family and domain databases

    Gene3Di 3.40.50.1260. 1 hit.
    3.40.50.1270. 1 hit.
    HAMAPi MF_00145. Phosphoglyc_kinase.
    InterProi IPR027250. Pgk_euglenozoa.
    IPR001576. Phosphoglycerate_kinase.
    IPR015901. Phosphoglycerate_kinase_C.
    IPR015911. Phosphoglycerate_kinase_CS.
    IPR015824. Phosphoglycerate_kinase_N.
    [Graphical view ]
    PANTHERi PTHR11406. PTHR11406. 1 hit.
    Pfami PF00162. PGK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000724. Pgk. 1 hit.
    PIRSF500126. Pgk_euglenozoa. 1 hit.
    PRINTSi PR00477. PHGLYCKINASE.
    SUPFAMi SSF53748. SSF53748. 1 hit.
    PROSITEi PS00111. PGLYCERATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evidence for gene conversion between the phosphoglycerate kinase genes of Trypanosoma brucei."
      le Blancq S.M., Swinkels B.W., Gibson W.C., Borst P.
      J. Mol. Biol. 200:439-447(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELES 2 AND 4).
    2. "Topogenesis of microbody enzymes: a sequence comparison of the genes for the glycosomal (microbody) and cytosolic phosphoglycerate kinases of Trypanosoma brucei."
      Osinga K.A., Swinkels B.W., Gibson W.C., Borst P., Veeneman G.H., van Boom J.H., Michels P.A.M., Opperdoes F.R.
      EMBO J. 4:3811-3817(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Synergistic effects of substrate-induced conformational changes in phosphoglycerate kinase activation."
      Bernstein B.E., Michels P.A.M., Hol W.G.J.
      Nature 385:275-278(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    4. "A bisubstrate analog induces unexpected conformational changes in phosphoglycerate kinase from Trypanosoma brucei."
      Bernstein B.E., Williams D.M., Bressi J.C., Kuhn P., Gelb M.H., Blackburn G.M., Hol W.G.J.
      J. Mol. Biol. 279:1137-1148(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 5-419 IN COMPLEX WITH ADP AND SUBSTRATE.

    Entry informationi

    Entry nameiPGKC_TRYBB
    AccessioniPrimary (citable) accession number: P07378
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    In T.brucei, three genes code for phosphoglycerate kinase isozymes, which are transported to different cell compartments.

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3