P07377 (PGKB_TRYBB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Phosphoglycerate kinase, cytosolic EC=2.7.2.3 Alternative name(s): PGK B allele 2 Phosphoglycerate kinase B
Organism	Trypanosoma brucei brucei
Taxonomic identifier	5702 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma › Trypanozoon › Trypanosoma brucei

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Miscellaneous	In T.brucei, three genes code for phosphoglycerate kinase isozymes, which are transported to different cell compartments.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Phosphoglycerate kinase, cytosolic		PRO_0000145864
Regions
	Nucleotide binding	376 – 379	4	ATP By similarity
	Region	24 – 26	3	Substrate binding By similarity
	Region	62 – 65	4	Substrate binding By similarity
Sites
	Binding site	39	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	173	1	Substrate By similarity
	Binding site	224	1	ATP By similarity
	Binding site	346	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07377 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 33AE32B02B51D1C5	FASTA	421	45,154
	10 20 30 40 50 60 MSLKERKSIN ECDLKGKKVL IRVDFNVPLD DGNITNDYRI RSALPAVQKV LTEGGSCVLM 70 80 90 100 110 120 SHLGRPKGVS MAEGKELGSA GGIPGFEQKA TLKPVAKALS ELLSRPVTFA PDCLNAADVV 130 140 150 160 170 180 SKMSPGDVVL LENVRFYKEE GSKKSTEERE AMAKILSSYG DVYISDAFGT AHRDSATMTG 190 200 210 220 230 240 IPKILGNGAA GYLMEKEISY FAKVLGNPPR PLVAIVGGAK VSDKIQLLDN MLQRIDYLLI 250 260 270 280 290 300 GGAMAYTFLK AQGYSIGISM CEESKLEFAR SLLKKAEDRK VQIILPIDHV CHTEFKAVDS 310 320 330 340 350 360 PLITEDQNIP EGHMALDIGP KTIEKYVQTI GKCKSAIWNG PMGVFEMVPY SKGTFAIAKA 370 380 390 400 410 420 MGRGTHEHGL MSIIGGGDSA SAAELSGEAK RMSHVSTGGG ASLELLEGKT LPGVTVLDDK E

References

[1]	"Evidence for gene conversion between the phosphoglycerate kinase genes of Trypanosoma brucei." le Blancq S.M., Swinkels B.W., Gibson W.C., Borst P. J. Mol. Biol. 200:439-447(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Topogenesis of microbody enzymes: a sequence comparison of the genes for the glycosomal (microbody) and cytosolic phosphoglycerate kinases of Trypanosoma brucei." Osinga K.A., Swinkels B.W., Gibson W.C., Borst P., Veeneman G.H., van Boom J.H., Michels P.A.M., Opperdoes F.R. EMBO J. 4:3811-3817(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03370 Genomic DNA. Translation: CAA27068.1. X05889 Genomic DNA. Translation: CAA29317.1.
PIR	KIUTGC. A25119.
3D structure databases
ProteinModelPortal	P07377.
SMR	P07377. Positions 5-420.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
SABIO-RK	P07377.
UniPathway	UPA00109; UER00185.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
InterPro	IPR027250. Pgk_euglenozoa. IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit. PIRSF500126. Pgk_euglenozoa. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGKB_TRYBB

Accession

Primary (citable) accession number: P07377

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)