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P07377 (PGKB_TRYBB) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase, cytosolic

EC=2.7.2.3
Alternative name(s):
PGK B allele 2
Phosphoglycerate kinase B
OrganismTrypanosoma brucei brucei
Taxonomic identifier5702 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Protein attributes

Sequence length421 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP-Rule MF_00145.

Miscellaneous

In T.brucei, three genes code for phosphoglycerate kinase isozymes, which are transported to different cell compartments.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 421421Phosphoglycerate kinase, cytosolic HAMAP-Rule MF_00145
PRO_0000145864

Regions

Nucleotide binding376 – 3794ATP By similarity
Region24 – 263Substrate binding By similarity
Region62 – 654Substrate binding By similarity

Sites

Binding site391Substrate By similarity
Binding site1351Substrate By similarity
Binding site1731Substrate By similarity
Binding site2241ATP By similarity
Binding site3461ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P07377 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 33AE32B02B51D1C5

FASTA42145,154
        10         20         30         40         50         60 
MSLKERKSIN ECDLKGKKVL IRVDFNVPLD DGNITNDYRI RSALPAVQKV LTEGGSCVLM 

        70         80         90        100        110        120 
SHLGRPKGVS MAEGKELGSA GGIPGFEQKA TLKPVAKALS ELLSRPVTFA PDCLNAADVV 

       130        140        150        160        170        180 
SKMSPGDVVL LENVRFYKEE GSKKSTEERE AMAKILSSYG DVYISDAFGT AHRDSATMTG 

       190        200        210        220        230        240 
IPKILGNGAA GYLMEKEISY FAKVLGNPPR PLVAIVGGAK VSDKIQLLDN MLQRIDYLLI 

       250        260        270        280        290        300 
GGAMAYTFLK AQGYSIGISM CEESKLEFAR SLLKKAEDRK VQIILPIDHV CHTEFKAVDS 

       310        320        330        340        350        360 
PLITEDQNIP EGHMALDIGP KTIEKYVQTI GKCKSAIWNG PMGVFEMVPY SKGTFAIAKA 

       370        380        390        400        410        420 
MGRGTHEHGL MSIIGGGDSA SAAELSGEAK RMSHVSTGGG ASLELLEGKT LPGVTVLDDK 


E 

« Hide

References

[1]"Evidence for gene conversion between the phosphoglycerate kinase genes of Trypanosoma brucei."
le Blancq S.M., Swinkels B.W., Gibson W.C., Borst P.
J. Mol. Biol. 200:439-447(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Topogenesis of microbody enzymes: a sequence comparison of the genes for the glycosomal (microbody) and cytosolic phosphoglycerate kinases of Trypanosoma brucei."
Osinga K.A., Swinkels B.W., Gibson W.C., Borst P., Veeneman G.H., van Boom J.H., Michels P.A.M., Opperdoes F.R.
EMBO J. 4:3811-3817(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03370 Genomic DNA. Translation: CAA27068.1.
X05889 Genomic DNA. Translation: CAA29317.1.
PIRKIUTGC. A25119.

3D structure databases

ProteinModelPortalP07377.
SMRP07377. Positions 5-420.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP07377.
UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR027250. Pgk_euglenozoa.
IPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PIRSF500126. Pgk_euglenozoa. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGKB_TRYBB
AccessionPrimary (citable) accession number: P07377
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways