Reviewed, UniProtKB/Swiss-Prot P07364 (CHER_ECOLI)

Names and origin

Protein names

Recommended name:
    Chemotaxis protein methyltransferase
    EC=2.1.1.80

Gene names

Name:	cheR
Synonyms:	cheX
Ordered Locus Names:	b1884, JW1873

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Methylation of the membrane-bound methyl-accepting chemotaxis proteins (MCP) to form gamma-glutamyl methyl ester residues in MCP.
Catalytic activity	S-adenosyl-L-methionine + protein L-glutamate = S-adenosyl-L-homocysteine + protein L-glutamate methyl ester.
Sequence similarities	Contains 1 cheR-type methyltransferase domain.

Ontologies

Keywords
Biological process	Chemotaxis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein-glutamate O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Chemotaxis protein methyltransferase		PRO_0000176033
Regions
	Domain	15 – 286	272	CheR-type methyltransferase
	Region	212 – 213	2	S-adenosyl-L-methionine binding By similarity
	Region	230 – 231	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	92	1	S-adenosyl-L-methionine By similarity
	Binding site	94	1	S-adenosyl-L-methionine By similarity
	Binding site	98	1	S-adenosyl-L-methionine By similarity
	Binding site	129	1	S-adenosyl-L-methionine By similarity
	Binding site	154	1	S-adenosyl-L-methionine By similarity
Experimental info
	Sequence conflict	113	1	R → G in AAA23568. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P07364-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 2AFA307DD406B135	FASTA	286	32,849
	10 20 30 40 50 60 MTSSLPCGQT SLLLQMTERL ALSDAHFRRI SQLIYQRAGI VLADHKRDMV YNRLVRRLRS 70 80 90 100 110 120 LGLTDFGHYL NLLESNQHSG EWQAFINSLT TNLTAFFREA HHFPLLADHA RRRSGEYRVW 130 140 150 160 170 180 SAAASTGEEP YSIAMTLADT LGTAPGRWKV FASDIDTEVL EKARSGIYRH EELKNLTPQQ 190 200 210 220 230 240 LQRYFMRGTG PHEGLVRVRQ ELANYVDFAP LNLLAKQYTV PGPFDAIFCR NVMIYFDQTT 250 260 270 280 QQEILRRFVP LLKPDGLLFA GHSENFSHLE RRFTLRGQTV YALSKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence corresponding to five chemotaxis genes in Escherichia coli." Mutoh N., Simon M.I. J. Bacteriol. 165:161-166(1986) [PubMed: 3510184] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M13463 Genomic DNA. Translation: AAA23568.1. U00096 Genomic DNA. Translation: AAC74954.1. AP009048 Genomic DNA. Translation: BAA15700.1.
PIR	XYECCR. D64951.
RefSeq	AP_002504.1. NP_416398.1.
3D structure databases
HSSP	HSSP built from PDB template 1AF7 based on UniProtKB P07801.
SMR	P07364. Positions 11-284.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9273N.
Genome annotation databases
GeneID	946396.
GenomeReviews	Gene locus JW1873 in contig AP009048_GR. Gene locus b1884 in contig U00096_GR.
KEGG	ecj:JW1873. eco:b1884.
Organism-specific databases
EchoBASE	EB0146.
EcoGene	EG10148. cheR.
CMR	Search...
Phylogenomic databases
HOGENOM	P07364.
OMA	P07364. IAGHSEN.
Enzyme and pathway databases
BioCyc	EcoCyc:CHER-MON.
Family and domain databases
InterPro	IPR000780. CheR_MeTrfase. [Graphical view]
Pfam	PF01739. CheR. 1 hit. PF03705. CheR_N. 1 hit. [Graphical view]
PRINTS	PR00996. CHERMTFRASE.
SMART	SM00138. MeTrc. 1 hit. [Graphical view]
PROSITE	PS50123. CHER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHER_ECOLI

Accession

Primary (citable) accession number: P07364
Secondary accession number(s): P78071

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)