Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P07360 (CO8G_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 153. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Complement component C8 gamma chain
Gene names
Name:C8G
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length202 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

C8 is a constituent of the membrane attack complex. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol.

Subunit structure

C8 is composed of three chains: alpha, beta and gamma. The alpha and gamma chains are disulfide bonded.

Subcellular location

Secreted.

Sequence similarities

Belongs to the calycin superfamily. Lipocalin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020
Chain21 – 202182Complement component C8 gamma chain
PRO_0000017881

Amino acid modifications

Modified residue211Pyrrolidone carboxylic acid
Disulfide bond60Interchain (with C-194 in C8-alpha chain) Ref.8 Ref.10 Ref.11 Ref.12
Disulfide bond96 ↔ 188 Ref.8 Ref.10 Ref.11 Ref.12

Natural variations

Natural variant691R → Q.
Corresponds to variant rs17614 [ dbSNP | Ensembl ].
VAR_014668
Natural variant1181D → G. Ref.1 Ref.2 Ref.3 Ref.5 Ref.6
Corresponds to variant rs7850844 [ dbSNP | Ensembl ].
VAR_044319
Natural variant1241H → N. Ref.6
Corresponds to variant rs17613 [ dbSNP | Ensembl ].
VAR_014669

Secondary structure

.................................. 202
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07360 [UniParc].

Last modified October 3, 2006. Version 3.
Checksum: 4E4F73140E607FFE

FASTA20222,277
        10         20         30         40         50         60 
MLPPGTATLL TLLLAAGSLG QKPQRPRRPA SPISTIQPKA NFDAQQFAGT WLLVAVGSAC 

        70         80         90        100        110        120 
RFLQEQGHRA EATTLHVAPQ GTAMAVSTFR KLDGICWQVR QLYGDTGVLG RFLLQARDAR 

       130        140        150        160        170        180 
GAVHVVVAET DYQSFAVLYL ERAGQLSVKL YARSLPVSDS VLSGFEQRVQ EAHLTEDQIF 

       190        200 
YFPKYGFCEA ADQFHVLDEV RR 

« Hide

References

« Hide 'large scale' references
[1]"The eighth component of human complement: evidence that it is an oligomeric serum protein assembled from products of three different genes."
Ng S.C., Rao A.G., Howard O.M.Z., Sodetz J.M.
Biochemistry 26:5229-5233(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, VARIANT GLY-118.
[2]"Structural homology of human complement component C8 gamma and plasma protein HC: identity of the cysteine bond pattern."
Haefliger J.-A., Jenne D.E., Stanley K.K., Tschopp J.
Biochem. Biophys. Res. Commun. 149:750-754(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLY-118.
Tissue: Liver.
[3]"Genomic structure of the human complement protein C8 gamma: homology to the lipocalin gene family."
Kaufman K.M., Sodetz J.M.
Biochemistry 33:5162-5166(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLY-118.
Tissue: Blood.
[4]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT GLY-118.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS GLY-118 AND ASN-124.
Tissue: Colon.
[7]"The homology of complement factor C8 gamma chain and alpha-1-microglobulin."
Hunt L.T., Elzanowski A., Barker W.C.
Biochem. Biophys. Res. Commun. 149:282-288(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: SIMILARITY TO LIPOCALINS.
[8]"Structural and functional characterization of complement C8 gamma, a member of the lipocalin protein family."
Haefliger J.-A., Peitsch M.C., Jenne D.E., Tschopp J.
Mol. Immunol. 28:123-131(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BONDS, RETINOL-BINDING, 3D-STRUCTURE MODELING.
[9]"Human complement protein C8 gamma."
Schreck S.F., Parker C., Plumb M.E., Sodetz J.M.
Biochim. Biophys. Acta 1482:199-208(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[10]"Crystal structure of human complement protein C8gamma at 1.2 A resolution reveals a lipocalin fold and a distinct ligand binding site."
Ortlund E., Parker C.L., Schreck S.F., Ginell S., Minor W., Sodetz J.M., Lebioda L.
Biochemistry 41:7030-7037(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.2 ANGSTROMS) OF 21-202, DISULFIDE BOND.
[11]"Structural features of the ligand binding site on human complement protein C8gamma: a member of the lipocalin family."
Chiswell B., Lovelace L.L., Brannen C., Ortlund E.A., Lebioda L., Sodetz J.M.
Biochim. Biophys. Acta 1774:637-644(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 21-202, DISULFIDE BOND.
[12]"Crystal structure of complement protein C8gamma in complex with a peptide containing the C8gamma binding site on C8alpha: implications for C8gamma ligand binding."
Lovelace L.L., Chiswell B., Slade D.J., Sodetz J.M., Lebioda L.
Mol. Immunol. 45:750-756(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.81 ANGSTROMS) OF 30-202, DISULFIDE BOND.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M17263 mRNA. Translation: AAA51888.1.
M17999 mRNA. Translation: AAA51863.1.
X06465 mRNA. Translation: CAA29773.1.
U08198 Genomic DNA. Translation: AAA18482.1.
AL807752 Genomic DNA. Translation: CAI12749.1.
CH471090 Genomic DNA. Translation: EAW88316.1.
BC113624 mRNA. Translation: AAI13625.1.
BC113626 mRNA. Translation: AAI13627.1.
PIRC8HUG. A27487.
RefSeqNP_000597.2. NM_000606.2.
UniGeneHs.1285.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IW2X-ray1.90A21-202[»]
1LF7X-ray1.20A21-202[»]
2OVAX-ray1.50A21-202[»]
2OVDX-ray1.80A21-202[»]
2OVEX-ray2.00A21-202[»]
2QOSX-ray1.81C30-202[»]
2RD7X-ray2.15C21-202[»]
3OJYX-ray2.51C21-202[»]
ProteinModelPortalP07360.
SMRP07360. Positions 30-202.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107194. 1 interaction.
STRING9606.ENSP00000224181.

PTM databases

PhosphoSiteP07360.

Proteomic databases

PaxDbP07360.
PeptideAtlasP07360.
PRIDEP07360.

Protocols and materials databases

DNASU733.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000224181; ENSP00000224181; ENSG00000176919.
GeneID733.
KEGGhsa:733.
UCSCuc004cka.2. human.

Organism-specific databases

CTD733.
GeneCardsGC09P139839.
H-InvDBHIX0201411.
HGNCHGNC:1354. C8G.
HPAHPA046269.
MIM120930. gene.
neXtProtNX_P07360.
Orphanet169150. Immunodeficiency due to a late component of complements deficiency.
PharmGKBPA25953.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG39544.
HOGENOMHOG000111143.
HOVERGENHBG005369.
InParanoidP07360.
KOK03999.
OMADAQQFAG.
PhylomeDBP07360.
TreeFamTF336103.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressP07360.
BgeeP07360.
CleanExHS_C8G.
GenevestigatorP07360.

Family and domain databases

Gene3D2.40.128.20. 1 hit.
InterProIPR002968. A1-microglobln.
IPR012674. Calycin.
IPR011038. Calycin-like.
IPR002345. Lipocalin.
IPR022272. Lipocalin_CS.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
[Graphical view]
PfamPF00061. Lipocalin. 1 hit.
[Graphical view]
PRINTSPR01215. A1MCGLOBULIN.
PR00179. LIPOCALIN.
SUPFAMSSF50814. SSF50814. 1 hit.
PROSITEPS00213. LIPOCALIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP07360.
GenomeRNAi733.
NextBio2984.
PROP07360.
SOURCESearch...

Entry information

Entry nameCO8G_HUMAN
AccessionPrimary (citable) accession number: P07360
Secondary accession number(s): Q14CT8, Q14CU0, Q5SQ07
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: October 3, 2006
Last modified: April 16, 2014
This is version 153 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM