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P07356 (ANXA2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 147. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Annexin A2
Alternative name(s):
Annexin II
Annexin-2
Calpactin I heavy chain
Calpactin-1 heavy chain
Chromobindin-8
Lipocortin II
Placental anticoagulant protein IV
Short name=PAP-IV
Protein I
p36
Gene names
Name:Anxa2
Synonyms:Anx2, Cal1h
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length339 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity.

Subunit structure

Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with DYSF. Interacts with ATP1B1 By similarity. Interacts with COCH By similarity. Ref.5

Subcellular location

Secretedextracellular spaceextracellular matrixbasement membrane. Melanosome By similarity. Note: In the lamina beneath the plasma membrane.

Domain

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Post-translational modification

ISGylated. Ref.6

Miscellaneous

It may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.

Sequence similarities

Belongs to the annexin family.

Contains 4 annexin repeats.

Ontologies

Keywords
   Cellular componentBasement membrane
Extracellular matrix
Secreted
   DomainAnnexin
Repeat
   LigandCalcium
Calcium/phospholipid-binding
   PTMAcetylation
Phosphoprotein
Ubl conjugation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processangiogenesis

Inferred from mutant phenotype PubMed 14702107. Source: MGI

body fluid secretion

Inferred from electronic annotation. Source: Ensembl

cellular response to acid

Inferred from direct assay PubMed 17690254. Source: MGI

collagen fibril organization

Inferred from mutant phenotype PubMed 14702107. Source: MGI

fibrinolysis

Inferred from mutant phenotype PubMed 14702107. Source: MGI

positive regulation of binding

Inferred from mutant phenotype PubMed 17690254. Source: MGI

positive regulation of fibroblast proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of vesicle fusion

Inferred from electronic annotation. Source: Ensembl

protein targeting to plasma membrane

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentSchmidt-Lanterman incisure

Inferred from direct assay PubMed 20237282. Source: BHF-UCL

basement membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cell cortex

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from direct assay PubMed 15078881. Source: MGI

early endosome

Inferred from direct assay PubMed 15078881. Source: MGI

extracellular vesicular exosome

Inferred from electronic annotation. Source: Ensembl

extrinsic component of plasma membrane

Inferred from direct assay PubMed 17690254. Source: MGI

lipid particle

Inferred from electronic annotation. Source: Ensembl

macropinosome

Inferred from electronic annotation. Source: Ensembl

melanosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

midbody

Inferred from electronic annotation. Source: Ensembl

myelin sheath adaxonal region

Inferred from direct assay PubMed 20237282. Source: BHF-UCL

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

protein complex

Inferred from electronic annotation. Source: Ensembl

ruffle

Inferred from electronic annotation. Source: Ensembl

sarcolemma

Inferred from direct assay Ref.5. Source: MGI

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

calcium-dependent phospholipid binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphatidylinositol-4,5-bisphosphate binding

Inferred from electronic annotation. Source: Ensembl

phospholipase inhibitor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

S100a10P082072EBI-738510,EBI-643986

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 339338Annexin A2
PRO_0000067471

Regions

Repeat42 – 10261Annexin 1
Repeat114 – 17461Annexin 2
Repeat199 – 25961Annexin 3
Repeat274 – 33461Annexin 4
Region2 – 2423S100A10-binding site Potential

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue241Phosphotyrosine; by SRC By similarity
Modified residue261Phosphoserine; by PKC By similarity
Modified residue491N6-acetyllysine Ref.9
Modified residue1521N6-acetyllysine Ref.9
Modified residue1991Phosphotyrosine Ref.8
Modified residue2271N6-acetyllysine Ref.9

Secondary structure

............................................... 339
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07356 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 4407E572097FB2C0

FASTA33938,676
        10         20         30         40         50         60 
MSTVHEILCK LSLEGDHSTP PSAYGSVKPY TNFDAERDAL NIETAVKTKG VDEVTIVNIL 

        70         80         90        100        110        120 
TNRSNVQRQD IAFAYQRRTK KELPSALKSA LSGHLETVIL GLLKTPAQYD ASELKASMKG 

       130        140        150        160        170        180 
LGTDEDSLIE IICSRTNQEL QEINRVYKEM YKTDLEKDII SDTSGDFRKL MVALAKGRRA 

       190        200        210        220        230        240 
EDGSVIDYEL IDQDARELYD AGVKRKGTDV PKWISIMTER SVCHLQKVFE RYKSYSPYDM 

       250        260        270        280        290        300 
LESIKKEVKG DLENAFLNLV QCIQNKPLYF ADRLYDSMKG KGTRDKVLIR IMVSRSEVDM 

       310        320        330 
LKIRSEFKRK YGKSLYYYIQ QDTKGDYQKA LLYLCGGDD 

« Hide

References

« Hide 'large scale' references
[1]"The cDNA sequence for the protein-tyrosine kinase substrate p36 (calpactin I heavy chain) reveals a multidomain protein with internal repeats."
Saris C.J.M., Tack B.F., Kristensen T., Glenney J.R. Jr., Hunter T.
Cell 46:201-212(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Structure and chromosome assignment of the murine p36 (calpactin I heavy chain) gene."
Amiguet P., D'Eustachio P., Kristensen T., Wetsel R.A., Saris C.J.M., Hunter T., Chaplin D.D., Tack B.F.
Biochemistry 29:1226-1232(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16 AND 322-339.
[4]"Complete structure of the murine p36 (annexin II) gene. Identification of mRNAs for both the murine and the human gene with alternatively spliced 5' noncoding exons."
Fey M.F., Moffat G.J., Vik D.P., Meisenhelder J., Saris C.J., Hunter T., Tack B.F.
Biochim. Biophys. Acta 1306:160-170(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16.
[5]"Dysferlin interacts with annexins A1 and A2 and mediates sarcolemmal wound-healing."
Lennon N.J., Kho A., Bacskai B.J., Perlmutter S.L., Hyman B.T., Brown R.H. Jr.
J. Biol. Chem. 278:50466-50473(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DYSF.
[6]"Proteomic identification of proteins conjugated to ISG15 in mouse and human cells."
Giannakopoulos N.V., Luo J.K., Papov V., Zou W., Lenschow D.J., Jacobs B.S., Borden E.C., Li J., Virgin H.W., Zhang D.E.
Biochem. Biophys. Res. Commun. 336:496-506(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ISGYLATION.
[7]"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-49; LYS-152 AND LYS-227, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Web resources

Protein Spotlight

Red velvet - Issue 86 of September 2007

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M14044 mRNA. Translation: AAA37360.1.
BC003327 mRNA. Translation: AAH03327.1.
BC005763 mRNA. Translation: AAH05763.1.
M33321 Genomic DNA. Translation: AAA37361.1.
M33322 Genomic DNA. Translation: AAA37362.1.
D10024 mRNA. Translation: BAA00914.1.
S82177 Genomic DNA. No translation available.
PIRLUMS36. A23943.
RefSeqNP_031611.1. NM_007585.3.
XP_006510859.1. XM_006510796.1.
UniGeneMm.238343.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4HREX-ray2.79A/B/C/D1-339[»]
ProteinModelPortalP07356.
SMRP07356. Positions 2-339.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198448. 7 interactions.
IntActP07356. 9 interactions.
MINTMINT-274998.

Protein family/group databases

TCDB9.A.48.1.1. the unconventional protein secretion (ups) system.

PTM databases

PhosphoSiteP07356.

2D gel databases

REPRODUCTION-2DPAGEIPI00468203.
P07356.
SWISS-2DPAGEP07356.

Proteomic databases

PaxDbP07356.
PRIDEP07356.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034756; ENSMUSP00000034756; ENSMUSG00000032231.
GeneID12306.
KEGGmmu:12306.
UCSCuc009qng.1. mouse.

Organism-specific databases

CTD302.
MGIMGI:88246. Anxa2.

Phylogenomic databases

eggNOGNOG259189.
HOGENOMHOG000158803.
HOVERGENHBG061815.
InParanoidP07356.
KOK17092.
OMAFIQQDTK.
OrthoDBEOG74XS72.
PhylomeDBP07356.
TreeFamTF105452.

Gene expression databases

ArrayExpressP07356.
BgeeP07356.
CleanExMM_ANXA2.
GenevestigatorP07356.

Family and domain databases

Gene3D1.10.220.10. 4 hits.
InterProIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002389. AnnexinII.
[Graphical view]
PANTHERPTHR10502:SF18. PTHR10502:SF18. 1 hit.
PfamPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSPR00196. ANNEXIN.
PR00198. ANNEXINII.
SMARTSM00335. ANX. 4 hits.
[Graphical view]
PROSITEPS00223. ANNEXIN. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio280840.
PROP07356.
SOURCESearch...

Entry information

Entry nameANXA2_MOUSE
AccessionPrimary (citable) accession number: P07356
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot