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Protein

Annexin A2

Gene

Anxa2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response (By similarity). Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9 (PubMed:22848640).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • angiogenesis Source: MGI
  • body fluid secretion Source: Ensembl
  • collagen fibril organization Source: MGI
  • fibrinolysis Source: MGI
  • membrane budding Source: MGI
  • membrane raft assembly Source: MGI
  • muscle contraction Source: Reactome
  • negative regulation of low-density lipoprotein particle receptor catabolic process Source: BHF-UCL
  • negative regulation of receptor binding Source: BHF-UCL
  • negative regulation of receptor internalization Source: BHF-UCL
  • osteoclast development Source: MGI
  • positive regulation of binding Source: MGI
  • positive regulation of fibroblast proliferation Source: Ensembl
  • positive regulation of protein phosphorylation Source: Ensembl
  • positive regulation of receptor activity Source: BHF-UCL
  • positive regulation of vesicle fusion Source: MGI
  • protein heterotetramerization Source: MGI
  • protein targeting to plasma membrane Source: Ensembl
  • response to thyroid hormone Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

Calcium, Calcium/phospholipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-445355. Smooth Muscle Contraction.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-75205. Dissolution of Fibrin Clot.

Protein family/group databases

TCDBi9.A.48.1.1. the unconventional protein secretion (ups) system.

Names & Taxonomyi

Protein namesi
Recommended name:
Annexin A2
Alternative name(s):
Annexin II
Annexin-2
Calpactin I heavy chain
Calpactin-1 heavy chain
Chromobindin-8
Lipocortin II
Placental anticoagulant protein IV
Short name:
PAP-IV
Protein I
p36
Gene namesi
Name:Anxa2
Synonyms:Anx2, Cal1h
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:88246. Anxa2.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: UniProtKB-SubCell
  • basolateral plasma membrane Source: BHF-UCL
  • cell-cell adherens junction Source: MGI
  • cell-cell junction Source: MGI
  • cell cortex Source: Ensembl
  • cell surface Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • early endosome Source: MGI
  • endosome Source: MGI
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • extrinsic component of plasma membrane Source: MGI
  • late endosome membrane Source: MGI
  • lipid particle Source: MGI
  • lysosomal membrane Source: MGI
  • macropinosome Source: Ensembl
  • melanosome Source: UniProtKB-SubCell
  • membrane Source: MGI
  • membrane raft Source: Ensembl
  • midbody Source: MGI
  • myelin sheath adaxonal region Source: BHF-UCL
  • nucleus Source: MGI
  • PCSK9-AnxA2 complex Source: BHF-UCL
  • perinuclear region of cytoplasm Source: Ensembl
  • plasma membrane Source: MGI
  • ruffle Source: Ensembl
  • sarcolemma Source: MGI
  • Schmidt-Lanterman incisure Source: BHF-UCL
  • vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

Pathology & Biotechi

Disruption phenotypei

Animals show a 40% increase of LDL-cholesterol levels in plasma.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000674712 – 339Annexin A2Add BLAST338

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei24Phosphotyrosine; by SRCBy similarity1
Modified residuei26Phosphoserine; by PKCBy similarity1
Modified residuei49N6-acetyllysine; alternateCombined sources1
Cross-linki49Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Modified residuei152N6-acetyllysineCombined sources1
Modified residuei184PhosphoserineBy similarity1
Modified residuei199PhosphotyrosineCombined sources1
Modified residuei227N6-acetyllysineCombined sources1

Post-translational modificationi

ISGylated.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP07356.
PaxDbiP07356.
PeptideAtlasiP07356.
PRIDEiP07356.

2D gel databases

REPRODUCTION-2DPAGEIPI00468203.
P07356.
SWISS-2DPAGEP07356.

PTM databases

iPTMnetiP07356.
PhosphoSitePlusiP07356.
SwissPalmiP07356.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032231.
CleanExiMM_ANXA2.
ExpressionAtlasiP07356. baseline and differential.
GenevisibleiP07356. MM.

Interactioni

Subunit structurei

Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36 (By similarity). Interacts with ATP1B1 (By similarity). Interacts with DYSF (PubMed:14506282). Interacts with COCH. Interacts (via repeat Annexin 1) with PCSK9 (via the C-terminal domain); the interaction inhibits the degradation of LDLR. Interacts with CEACAM1 (via the cytoplasmic domain); this interaction is regulated by phosphorylation of CEACAM1 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
S100a10P082072EBI-738510,EBI-643986

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198448. 20 interactors.
IntActiP07356. 17 interactors.
MINTiMINT-274998.
STRINGi10090.ENSMUSP00000034756.

Structurei

Secondary structure

1339
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 8Combined sources5
Helixi35 – 46Combined sources12
Helixi53 – 61Combined sources9
Helixi65 – 79Combined sources15
Helixi83 – 90Combined sources8
Helixi93 – 103Combined sources11
Helixi106 – 118Combined sources13
Helixi125 – 134Combined sources10
Helixi137 – 151Combined sources15
Helixi155 – 162Combined sources8
Helixi165 – 175Combined sources11
Helixi188 – 201Combined sources14
Beta strandi204 – 207Combined sources4
Helixi210 – 219Combined sources10
Helixi222 – 235Combined sources14
Helixi240 – 247Combined sources8
Helixi251 – 264Combined sources14
Helixi266 – 278Combined sources13
Beta strandi279 – 282Combined sources4
Helixi285 – 294Combined sources10
Turni296 – 299Combined sources4
Helixi300 – 311Combined sources12
Helixi315 – 322Combined sources8
Helixi325 – 335Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HREX-ray2.79A/B/C/D1-339[»]
ProteinModelPortaliP07356.
SMRiP07356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati42 – 102Annexin 1Add BLAST61
Repeati114 – 174Annexin 2Add BLAST61
Repeati199 – 259Annexin 3Add BLAST61
Repeati274 – 334Annexin 4Add BLAST61

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 24S100A10-binding siteSequence analysisAdd BLAST23

Domaini

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Sequence similaritiesi

Belongs to the annexin family.Curated
Contains 4 annexin repeats.Curated

Keywords - Domaini

Annexin, Repeat

Phylogenomic databases

eggNOGiKOG0819. Eukaryota.
ENOG410XPUN. LUCA.
GeneTreeiENSGT00760000118972.
HOGENOMiHOG000158803.
HOVERGENiHBG061815.
InParanoidiP07356.
KOiK17092.
OMAiFIQQDTK.
OrthoDBiEOG091G0H6H.
PhylomeDBiP07356.
TreeFamiTF105452.

Family and domain databases

Gene3Di1.10.220.10. 4 hits.
InterProiIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002389. AnnexinII.
[Graphical view]
PANTHERiPTHR10502:SF111. PTHR10502:SF111. 1 hit.
PfamiPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSiPR00196. ANNEXIN.
PR00198. ANNEXINII.
SMARTiSM00335. ANX. 4 hits.
[Graphical view]
PROSITEiPS00223. ANNEXIN. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07356-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTVHEILCK LSLEGDHSTP PSAYGSVKPY TNFDAERDAL NIETAVKTKG
60 70 80 90 100
VDEVTIVNIL TNRSNVQRQD IAFAYQRRTK KELPSALKSA LSGHLETVIL
110 120 130 140 150
GLLKTPAQYD ASELKASMKG LGTDEDSLIE IICSRTNQEL QEINRVYKEM
160 170 180 190 200
YKTDLEKDII SDTSGDFRKL MVALAKGRRA EDGSVIDYEL IDQDARELYD
210 220 230 240 250
AGVKRKGTDV PKWISIMTER SVCHLQKVFE RYKSYSPYDM LESIKKEVKG
260 270 280 290 300
DLENAFLNLV QCIQNKPLYF ADRLYDSMKG KGTRDKVLIR IMVSRSEVDM
310 320 330
LKIRSEFKRK YGKSLYYYIQ QDTKGDYQKA LLYLCGGDD
Length:339
Mass (Da):38,676
Last modified:January 23, 2007 - v2
Checksum:i4407E572097FB2C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14044 mRNA. Translation: AAA37360.1.
BC003327 mRNA. Translation: AAH03327.1.
BC005763 mRNA. Translation: AAH05763.1.
M33321 Genomic DNA. Translation: AAA37361.1.
M33322 Genomic DNA. Translation: AAA37362.1.
D10024 mRNA. Translation: BAA00914.1.
S82177 Genomic DNA. No translation available.
CCDSiCCDS23316.1.
PIRiA23943. LUMS36.
RefSeqiNP_031611.1. NM_007585.3.
XP_006510859.1. XM_006510796.2.
UniGeneiMm.238343.

Genome annotation databases

EnsembliENSMUST00000034756; ENSMUSP00000034756; ENSMUSG00000032231.
GeneIDi12306.
KEGGimmu:12306.
UCSCiuc009qng.1. mouse.

Cross-referencesi

Web resourcesi

Protein Spotlight

Red velvet - Issue 86 of September 2007

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14044 mRNA. Translation: AAA37360.1.
BC003327 mRNA. Translation: AAH03327.1.
BC005763 mRNA. Translation: AAH05763.1.
M33321 Genomic DNA. Translation: AAA37361.1.
M33322 Genomic DNA. Translation: AAA37362.1.
D10024 mRNA. Translation: BAA00914.1.
S82177 Genomic DNA. No translation available.
CCDSiCCDS23316.1.
PIRiA23943. LUMS36.
RefSeqiNP_031611.1. NM_007585.3.
XP_006510859.1. XM_006510796.2.
UniGeneiMm.238343.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HREX-ray2.79A/B/C/D1-339[»]
ProteinModelPortaliP07356.
SMRiP07356.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198448. 20 interactors.
IntActiP07356. 17 interactors.
MINTiMINT-274998.
STRINGi10090.ENSMUSP00000034756.

Protein family/group databases

TCDBi9.A.48.1.1. the unconventional protein secretion (ups) system.

PTM databases

iPTMnetiP07356.
PhosphoSitePlusiP07356.
SwissPalmiP07356.

2D gel databases

REPRODUCTION-2DPAGEIPI00468203.
P07356.
SWISS-2DPAGEP07356.

Proteomic databases

EPDiP07356.
PaxDbiP07356.
PeptideAtlasiP07356.
PRIDEiP07356.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034756; ENSMUSP00000034756; ENSMUSG00000032231.
GeneIDi12306.
KEGGimmu:12306.
UCSCiuc009qng.1. mouse.

Organism-specific databases

CTDi302.
MGIiMGI:88246. Anxa2.

Phylogenomic databases

eggNOGiKOG0819. Eukaryota.
ENOG410XPUN. LUCA.
GeneTreeiENSGT00760000118972.
HOGENOMiHOG000158803.
HOVERGENiHBG061815.
InParanoidiP07356.
KOiK17092.
OMAiFIQQDTK.
OrthoDBiEOG091G0H6H.
PhylomeDBiP07356.
TreeFamiTF105452.

Enzyme and pathway databases

ReactomeiR-MMU-445355. Smooth Muscle Contraction.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-75205. Dissolution of Fibrin Clot.

Miscellaneous databases

ChiTaRSiAnxa2. mouse.
PROiP07356.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032231.
CleanExiMM_ANXA2.
ExpressionAtlasiP07356. baseline and differential.
GenevisibleiP07356. MM.

Family and domain databases

Gene3Di1.10.220.10. 4 hits.
InterProiIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002389. AnnexinII.
[Graphical view]
PANTHERiPTHR10502:SF111. PTHR10502:SF111. 1 hit.
PfamiPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSiPR00196. ANNEXIN.
PR00198. ANNEXINII.
SMARTiSM00335. ANX. 4 hits.
[Graphical view]
PROSITEiPS00223. ANNEXIN. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANXA2_MOUSE
AccessioniPrimary (citable) accession number: P07356
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 176 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

It may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.