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P07347

- ARD1_YEAST

UniProt

P07347 - ARD1_YEAST

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Protein

N-terminal acetyltransferase A complex catalytic subunit ARD1

Gene
ARD1, YHR013C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic component of the NatA N-terminal acetyltransferase, which catalyzes acetylation of proteins beginning with Met-Ser, Met-Gly and Met-Ala. N-acetylation plays a role in normal eukaryotic translation and processing, protect against proteolytic degradation and protein turnover.1 Publication

Catalytic activityi

Acetyl-CoA + peptide = N(alpha)-acetylpeptide + CoA.

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. peptide alpha-N-acetyltransferase activity Source: UniProtKB-EC
  3. peptide alpha-N-propionyltransferase activity Source: SGD
  4. protein binding Source: IntAct

GO - Biological processi

  1. histone acetylation Source: SGD
  2. N-terminal protein amino acid acetylation Source: SGD
  3. N-terminal protein amino acid propionylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YHR013C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-terminal acetyltransferase A complex catalytic subunit ARD1 (EC:2.3.1.88)
Short name:
NatA complex subunit ARD1
Alternative name(s):
Arrest-defective protein 1
Gene namesi
Name:ARD1
Ordered Locus Names:YHR013C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR013c.
SGDiS000001055. ARD1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. NatA complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 238238N-terminal acetyltransferase A complex catalytic subunit ARD1PRO_0000074529Add
BLAST

Proteomic databases

MaxQBiP07347.
PaxDbiP07347.
PeptideAtlasiP07347.

Expressioni

Gene expression databases

GenevestigatoriP07347.

Interactioni

Subunit structurei

Component of the N-terminal acetyltransferase A (NatA) complex, which is composed of ARD1, NAT1 and NAT5. Can self-associate.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-2796,EBI-2796
NAT1P1294511EBI-2796,EBI-11868

Protein-protein interaction databases

BioGridi36440. 317 interactions.
DIPiDIP-6788N.
IntActiP07347. 31 interactions.
MINTiMINT-617928.
STRINGi4932.YHR013C.

Structurei

Secondary structure

1
238
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74
Helixi10 – 123
Helixi14 – 174
Helixi19 – 213
Helixi27 – 293
Helixi30 – 3910
Turni41 – 433
Beta strandi45 – 506
Helixi66 – 694
Turni70 – 723
Beta strandi85 – 873
Beta strandi90 – 10011
Helixi104 – 1063
Beta strandi107 – 1093
Beta strandi112 – 1209
Helixi122 – 1243
Beta strandi126 – 1283
Helixi129 – 14517
Beta strandi149 – 1557
Helixi159 – 1668
Turni167 – 1693
Beta strandi172 – 1776
Beta strandi187 – 1937
Helixi196 – 1994
Helixi201 – 2044
Turni205 – 2084
Beta strandi220 – 2223
Helixi224 – 23310

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HNWX-ray2.80B1-238[»]
4HNXX-ray2.34B1-238[»]
4HNYX-ray2.25B/D1-238[»]
ProteinModelPortaliP07347.
SMRiP07347. Positions 2-235.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 195161N-acetyltransferaseAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0456.
GeneTreeiENSGT00550000074803.
HOGENOMiHOG000078523.
KOiK00670.
OMAiICMQNAN.
OrthoDBiEOG71K6FX.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 2 hits.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07347-1 [UniParc]FASTAAdd to Basket

« Hide

MPINIRRATI NDIICMQNAN LHNLPENYMM KYYMYHILSW PEASFVATTT    50
TLDCEDSDEQ DENDKLELTL DGTNDGRTIK LDPTYLAPGE KLVGYVLVKM 100
NDDPDQQNEP PNGHITSLSV MRTYRRMGIA ENLMRQALFA LREVHQAEYV 150
SLHVRQSNRA ALHLYRDTLA FEVLSIEKSY YQDGEDAYAM KKVLKLEELQ 200
ISNFTHRRLK ENEEKLEDDL ESDLLEDIIK QGVNDIIV 238
Length:238
Mass (Da):27,603
Last modified:February 1, 1995 - v2
Checksum:iAEB02BA5012D1137
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371I → T in AAA66323. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11621 Genomic DNA. Translation: AAA66323.1.
U10400 Genomic DNA. Translation: AAB68937.1.
AY557822 Genomic DNA. Translation: AAS56148.1.
BK006934 Genomic DNA. Translation: DAA06702.1.
PIRiS46783. TWBYA1.
RefSeqiNP_011877.1. NM_001179143.1.

Genome annotation databases

EnsemblFungiiYHR013C; YHR013C; YHR013C.
GeneIDi856404.
KEGGisce:YHR013C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11621 Genomic DNA. Translation: AAA66323.1 .
U10400 Genomic DNA. Translation: AAB68937.1 .
AY557822 Genomic DNA. Translation: AAS56148.1 .
BK006934 Genomic DNA. Translation: DAA06702.1 .
PIRi S46783. TWBYA1.
RefSeqi NP_011877.1. NM_001179143.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HNW X-ray 2.80 B 1-238 [» ]
4HNX X-ray 2.34 B 1-238 [» ]
4HNY X-ray 2.25 B/D 1-238 [» ]
ProteinModelPortali P07347.
SMRi P07347. Positions 2-235.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36440. 317 interactions.
DIPi DIP-6788N.
IntActi P07347. 31 interactions.
MINTi MINT-617928.
STRINGi 4932.YHR013C.

Proteomic databases

MaxQBi P07347.
PaxDbi P07347.
PeptideAtlasi P07347.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR013C ; YHR013C ; YHR013C .
GeneIDi 856404.
KEGGi sce:YHR013C.

Organism-specific databases

CYGDi YHR013c.
SGDi S000001055. ARD1.

Phylogenomic databases

eggNOGi COG0456.
GeneTreei ENSGT00550000074803.
HOGENOMi HOG000078523.
KOi K00670.
OMAi ICMQNAN.
OrthoDBi EOG71K6FX.

Enzyme and pathway databases

BioCyci YEAST:YHR013C-MONOMER.

Miscellaneous databases

NextBioi 981943.

Gene expression databases

Genevestigatori P07347.

Family and domain databases

Gene3Di 3.40.630.30. 2 hits.
InterProi IPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view ]
Pfami PF00583. Acetyltransf_1. 1 hit.
[Graphical view ]
SUPFAMi SSF55729. SSF55729. 2 hits.
PROSITEi PS51186. GNAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The ARD1 gene of yeast functions in the switch between the mitotic cell cycle and alternative developmental pathways."
    Whiteway M., Szostak J.W.
    Cell 43:483-492(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Identification and characterization of genes and mutants for an N-terminal acetyltransferase from yeast."
    Mullen J.R., Kayne P.S., Moerschell R.P., Tsunasawa S., Gribskov M., Colavito-Shepanski M., Grunstein M., Sherman F., Sternglanz R.
    EMBO J. 8:2067-2075(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "ARD1 and NAT1 proteins form a complex that has N-terminal acetyltransferase activity."
    Park E.C., Szostak J.W.
    EMBO J. 11:2087-2093(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NAT1, SELF-ASSOCIATION.
  7. "The yeast N(alpha)-acetyltransferase NatA is quantitatively anchored to the ribosome and interacts with nascent polypeptides."
    Gautschi M., Just S., Mun A., Ross S., Rucknagel P., Dubaquie Y., Ehrenhofer-Murray A., Rospert S.
    Mol. Cell. Biol. 23:7403-7414(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE NATA COMPLEX.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiARD1_YEAST
AccessioniPrimary (citable) accession number: P07347
Secondary accession number(s): D3DKV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1995
Last modified: July 9, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3310 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

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