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P07347 (ARD1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-terminal acetyltransferase A complex catalytic subunit ARD1

Short name=NatA complex subunit ARD1
EC=2.3.1.88
Alternative name(s):
Arrest-defective protein 1
Gene names
Name:ARD1
Ordered Locus Names:YHR013C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length238 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalytic component of the NatA N-terminal acetyltransferase, which catalyzes acetylation of proteins beginning with Met-Ser, Met-Gly and Met-Ala. N-acetylation plays a role in normal eukaryotic translation and processing, protect against proteolytic degradation and protein turnover. Ref.6

Catalytic activity

Acetyl-CoA + peptide = N(alpha)-acetylpeptide + CoA.

Subunit structure

Component of the N-terminal acetyltransferase A (NatA) complex, which is composed of ARD1, NAT1 and NAT5. Can self-associate. Ref.7

Subcellular location

Cytoplasm Ref.8.

Miscellaneous

Present with 3310 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the acetyltransferase family. ARD1 subfamily.

Contains 1 N-acetyltransferase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-2796,EBI-2796
NAT1P1294511EBI-2796,EBI-11868

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 238238N-terminal acetyltransferase A complex catalytic subunit ARD1
PRO_0000074529

Regions

Domain35 – 195161N-acetyltransferase

Experimental info

Sequence conflict371I → T in AAA66323. Ref.1

Secondary structure

................................................... 238
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07347 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: AEB02BA5012D1137

FASTA23827,603
        10         20         30         40         50         60 
MPINIRRATI NDIICMQNAN LHNLPENYMM KYYMYHILSW PEASFVATTT TLDCEDSDEQ 

        70         80         90        100        110        120 
DENDKLELTL DGTNDGRTIK LDPTYLAPGE KLVGYVLVKM NDDPDQQNEP PNGHITSLSV 

       130        140        150        160        170        180 
MRTYRRMGIA ENLMRQALFA LREVHQAEYV SLHVRQSNRA ALHLYRDTLA FEVLSIEKSY 

       190        200        210        220        230 
YQDGEDAYAM KKVLKLEELQ ISNFTHRRLK ENEEKLEDDL ESDLLEDIIK QGVNDIIV 

« Hide

References

« Hide 'large scale' references
[1]"The ARD1 gene of yeast functions in the switch between the mitotic cell cycle and alternative developmental pathways."
Whiteway M., Szostak J.W.
Cell 43:483-492(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Identification and characterization of genes and mutants for an N-terminal acetyltransferase from yeast."
Mullen J.R., Kayne P.S., Moerschell R.P., Tsunasawa S., Gribskov M., Colavito-Shepanski M., Grunstein M., Sherman F., Sternglanz R.
EMBO J. 8:2067-2075(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"ARD1 and NAT1 proteins form a complex that has N-terminal acetyltransferase activity."
Park E.C., Szostak J.W.
EMBO J. 11:2087-2093(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NAT1, SELF-ASSOCIATION.
[7]"The yeast N(alpha)-acetyltransferase NatA is quantitatively anchored to the ribosome and interacts with nascent polypeptides."
Gautschi M., Just S., Mun A., Ross S., Rucknagel P., Dubaquie Y., Ehrenhofer-Murray A., Rospert S.
Mol. Cell. Biol. 23:7403-7414(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE NATA COMPLEX.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M11621 Genomic DNA. Translation: AAA66323.1.
U10400 Genomic DNA. Translation: AAB68937.1.
AY557822 Genomic DNA. Translation: AAS56148.1.
BK006934 Genomic DNA. Translation: DAA06702.1.
PIRTWBYA1. S46783.
RefSeqNP_011877.1. NM_001179143.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4HNWX-ray2.80B1-238[»]
4HNXX-ray2.34B1-238[»]
4HNYX-ray2.25B/D1-238[»]
ProteinModelPortalP07347.
SMRP07347. Positions 2-235.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36440. 317 interactions.
DIPDIP-6788N.
IntActP07347. 31 interactions.
MINTMINT-617928.
STRING4932.YHR013C.

Proteomic databases

MaxQBP07347.
PaxDbP07347.
PeptideAtlasP07347.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR013C; YHR013C; YHR013C.
GeneID856404.
KEGGsce:YHR013C.

Organism-specific databases

CYGDYHR013c.
SGDS000001055. ARD1.

Phylogenomic databases

eggNOGCOG0456.
GeneTreeENSGT00550000074803.
HOGENOMHOG000078523.
KOK00670.
OMAICMQNAN.
OrthoDBEOG71K6FX.

Enzyme and pathway databases

BioCycYEAST:YHR013C-MONOMER.

Gene expression databases

GenevestigatorP07347.

Family and domain databases

Gene3D3.40.630.30. 2 hits.
InterProIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMSSF55729. SSF55729. 2 hits.
PROSITEPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981943.

Entry information

Entry nameARD1_YEAST
AccessionPrimary (citable) accession number: P07347
Secondary accession number(s): D3DKV8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1995
Last modified: July 9, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references