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P07347

- ARD1_YEAST

UniProt

P07347 - ARD1_YEAST

Protein

N-terminal acetyltransferase A complex catalytic subunit ARD1

Gene

ARD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalytic component of the NatA N-terminal acetyltransferase, which catalyzes acetylation of proteins beginning with Met-Ser, Met-Gly and Met-Ala. N-acetylation plays a role in normal eukaryotic translation and processing, protect against proteolytic degradation and protein turnover.1 Publication

    Catalytic activityi

    Acetyl-CoA + peptide = N(alpha)-acetylpeptide + CoA.

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. peptide alpha-N-acetyltransferase activity Source: UniProtKB-EC
    3. peptide alpha-N-propionyltransferase activity Source: SGD
    4. protein binding Source: IntAct

    GO - Biological processi

    1. histone acetylation Source: SGD
    2. N-terminal protein amino acid acetylation Source: SGD
    3. N-terminal protein amino acid propionylation Source: SGD

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciYEAST:YHR013C-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-terminal acetyltransferase A complex catalytic subunit ARD1 (EC:2.3.1.88)
    Short name:
    NatA complex subunit ARD1
    Alternative name(s):
    Arrest-defective protein 1
    Gene namesi
    Name:ARD1
    Ordered Locus Names:YHR013C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR013c.
    SGDiS000001055. ARD1.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. NatA complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 238238N-terminal acetyltransferase A complex catalytic subunit ARD1PRO_0000074529Add
    BLAST

    Proteomic databases

    MaxQBiP07347.
    PaxDbiP07347.
    PeptideAtlasiP07347.

    Expressioni

    Gene expression databases

    GenevestigatoriP07347.

    Interactioni

    Subunit structurei

    Component of the N-terminal acetyltransferase A (NatA) complex, which is composed of ARD1, NAT1 and NAT5. Can self-associate.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-2796,EBI-2796
    NAT1P1294511EBI-2796,EBI-11868

    Protein-protein interaction databases

    BioGridi36440. 317 interactions.
    DIPiDIP-6788N.
    IntActiP07347. 31 interactions.
    MINTiMINT-617928.
    STRINGi4932.YHR013C.

    Structurei

    Secondary structure

    1
    238
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 74
    Helixi10 – 123
    Helixi14 – 174
    Helixi19 – 213
    Helixi27 – 293
    Helixi30 – 3910
    Turni41 – 433
    Beta strandi45 – 506
    Helixi66 – 694
    Turni70 – 723
    Beta strandi85 – 873
    Beta strandi90 – 10011
    Helixi104 – 1063
    Beta strandi107 – 1093
    Beta strandi112 – 1209
    Helixi122 – 1243
    Beta strandi126 – 1283
    Helixi129 – 14517
    Beta strandi149 – 1557
    Helixi159 – 1668
    Turni167 – 1693
    Beta strandi172 – 1776
    Beta strandi187 – 1937
    Helixi196 – 1994
    Helixi201 – 2044
    Turni205 – 2084
    Beta strandi220 – 2223
    Helixi224 – 23310

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4HNWX-ray2.80B1-238[»]
    4HNXX-ray2.34B1-238[»]
    4HNYX-ray2.25B/D1-238[»]
    ProteinModelPortaliP07347.
    SMRiP07347. Positions 2-235.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 195161N-acetyltransferasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0456.
    GeneTreeiENSGT00550000074803.
    HOGENOMiHOG000078523.
    KOiK00670.
    OMAiICMQNAN.
    OrthoDBiEOG71K6FX.

    Family and domain databases

    Gene3Di3.40.630.30. 2 hits.
    InterProiIPR016181. Acyl_CoA_acyltransferase.
    IPR000182. GNAT_dom.
    [Graphical view]
    PfamiPF00583. Acetyltransf_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF55729. SSF55729. 2 hits.
    PROSITEiPS51186. GNAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07347-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPINIRRATI NDIICMQNAN LHNLPENYMM KYYMYHILSW PEASFVATTT    50
    TLDCEDSDEQ DENDKLELTL DGTNDGRTIK LDPTYLAPGE KLVGYVLVKM 100
    NDDPDQQNEP PNGHITSLSV MRTYRRMGIA ENLMRQALFA LREVHQAEYV 150
    SLHVRQSNRA ALHLYRDTLA FEVLSIEKSY YQDGEDAYAM KKVLKLEELQ 200
    ISNFTHRRLK ENEEKLEDDL ESDLLEDIIK QGVNDIIV 238
    Length:238
    Mass (Da):27,603
    Last modified:February 1, 1995 - v2
    Checksum:iAEB02BA5012D1137
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti37 – 371I → T in AAA66323. (PubMed:3907857)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11621 Genomic DNA. Translation: AAA66323.1.
    U10400 Genomic DNA. Translation: AAB68937.1.
    AY557822 Genomic DNA. Translation: AAS56148.1.
    BK006934 Genomic DNA. Translation: DAA06702.1.
    PIRiS46783. TWBYA1.
    RefSeqiNP_011877.1. NM_001179143.1.

    Genome annotation databases

    EnsemblFungiiYHR013C; YHR013C; YHR013C.
    GeneIDi856404.
    KEGGisce:YHR013C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11621 Genomic DNA. Translation: AAA66323.1 .
    U10400 Genomic DNA. Translation: AAB68937.1 .
    AY557822 Genomic DNA. Translation: AAS56148.1 .
    BK006934 Genomic DNA. Translation: DAA06702.1 .
    PIRi S46783. TWBYA1.
    RefSeqi NP_011877.1. NM_001179143.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4HNW X-ray 2.80 B 1-238 [» ]
    4HNX X-ray 2.34 B 1-238 [» ]
    4HNY X-ray 2.25 B/D 1-238 [» ]
    ProteinModelPortali P07347.
    SMRi P07347. Positions 2-235.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36440. 317 interactions.
    DIPi DIP-6788N.
    IntActi P07347. 31 interactions.
    MINTi MINT-617928.
    STRINGi 4932.YHR013C.

    Proteomic databases

    MaxQBi P07347.
    PaxDbi P07347.
    PeptideAtlasi P07347.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR013C ; YHR013C ; YHR013C .
    GeneIDi 856404.
    KEGGi sce:YHR013C.

    Organism-specific databases

    CYGDi YHR013c.
    SGDi S000001055. ARD1.

    Phylogenomic databases

    eggNOGi COG0456.
    GeneTreei ENSGT00550000074803.
    HOGENOMi HOG000078523.
    KOi K00670.
    OMAi ICMQNAN.
    OrthoDBi EOG71K6FX.

    Enzyme and pathway databases

    BioCyci YEAST:YHR013C-MONOMER.

    Miscellaneous databases

    NextBioi 981943.

    Gene expression databases

    Genevestigatori P07347.

    Family and domain databases

    Gene3Di 3.40.630.30. 2 hits.
    InterProi IPR016181. Acyl_CoA_acyltransferase.
    IPR000182. GNAT_dom.
    [Graphical view ]
    Pfami PF00583. Acetyltransf_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55729. SSF55729. 2 hits.
    PROSITEi PS51186. GNAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ARD1 gene of yeast functions in the switch between the mitotic cell cycle and alternative developmental pathways."
      Whiteway M., Szostak J.W.
      Cell 43:483-492(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Identification and characterization of genes and mutants for an N-terminal acetyltransferase from yeast."
      Mullen J.R., Kayne P.S., Moerschell R.P., Tsunasawa S., Gribskov M., Colavito-Shepanski M., Grunstein M., Sherman F., Sternglanz R.
      EMBO J. 8:2067-2075(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "ARD1 and NAT1 proteins form a complex that has N-terminal acetyltransferase activity."
      Park E.C., Szostak J.W.
      EMBO J. 11:2087-2093(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH NAT1, SELF-ASSOCIATION.
    7. "The yeast N(alpha)-acetyltransferase NatA is quantitatively anchored to the ribosome and interacts with nascent polypeptides."
      Gautschi M., Just S., Mun A., Ross S., Rucknagel P., Dubaquie Y., Ehrenhofer-Murray A., Rospert S.
      Mol. Cell. Biol. 23:7403-7414(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE NATA COMPLEX.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiARD1_YEAST
    AccessioniPrimary (citable) accession number: P07347
    Secondary accession number(s): D3DKV8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 140 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3310 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3