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Protein

N-terminal acetyltransferase A complex catalytic subunit ARD1

Gene

ARD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic component of the NatA N-terminal acetyltransferase, which catalyzes acetylation of proteins beginning with Met-Ser, Met-Gly and Met-Ala. N-acetylation plays a role in normal eukaryotic translation and processing, protect against proteolytic degradation and protein turnover.1 Publication

Catalytic activityi

Acetyl-CoA + peptide = N(alpha)-acetylpeptide + CoA.

GO - Molecular functioni

GO - Biological processi

  • histone acetylation Source: SGD
  • N-terminal peptidyl-glutamic acid acetylation Source: GO_Central
  • N-terminal peptidyl-serine acetylation Source: GO_Central
  • N-terminal protein amino acid acetylation Source: SGD
  • N-terminal protein amino acid propionylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YHR013C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-terminal acetyltransferase A complex catalytic subunit ARD1 (EC:2.3.1.88)
Short name:
NatA complex subunit ARD1
Alternative name(s):
Arrest-defective protein 1
Gene namesi
Name:ARD1
Ordered Locus Names:YHR013C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR013C.
SGDiS000001055. ARD1.

Subcellular locationi

GO - Cellular componenti

  • NatA complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745291 – 238N-terminal acetyltransferase A complex catalytic subunit ARD1Add BLAST238

Proteomic databases

MaxQBiP07347.
PRIDEiP07347.

Interactioni

Subunit structurei

Component of the N-terminal acetyltransferase A (NatA) complex, which is composed of ARD1, NAT1 and NAT5. Can self-associate.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-2796,EBI-2796
NAT1P1294511EBI-2796,EBI-11868

Protein-protein interaction databases

BioGridi36440. 320 interactors.
DIPiDIP-6788N.
IntActiP07347. 32 interactors.
MINTiMINT-617928.

Structurei

Secondary structure

1238
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi10 – 12Combined sources3
Helixi13 – 23Combined sources11
Helixi27 – 29Combined sources3
Helixi30 – 39Combined sources10
Turni41 – 43Combined sources3
Beta strandi45 – 49Combined sources5
Helixi66 – 69Combined sources4
Turni70 – 72Combined sources3
Beta strandi85 – 87Combined sources3
Beta strandi90 – 100Combined sources11
Helixi104 – 106Combined sources3
Beta strandi107 – 109Combined sources3
Beta strandi112 – 120Combined sources9
Helixi122 – 124Combined sources3
Beta strandi126 – 128Combined sources3
Helixi129 – 145Combined sources17
Beta strandi148 – 155Combined sources8
Helixi159 – 166Combined sources8
Turni167 – 169Combined sources3
Beta strandi172 – 177Combined sources6
Beta strandi187 – 193Combined sources7
Helixi196 – 199Combined sources4
Helixi201 – 204Combined sources4
Turni205 – 208Combined sources4
Beta strandi220 – 222Combined sources3
Helixi224 – 233Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HNWX-ray2.80B1-238[»]
4HNXX-ray2.34B1-238[»]
4HNYX-ray2.25B/D1-238[»]
4XNHX-ray2.10B1-238[»]
4XPDX-ray2.81B1-238[»]
4Y49X-ray3.95B/H/N1-238[»]
ProteinModelPortaliP07347.
SMRiP07347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 195N-acetyltransferasePROSITE-ProRule annotationAdd BLAST161

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074803.
HOGENOMiHOG000078523.
InParanoidiP07347.
KOiK20791.
OMAiWPEASFV.
OrthoDBiEOG092C4MWD.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 2 hits.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07347-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPINIRRATI NDIICMQNAN LHNLPENYMM KYYMYHILSW PEASFVATTT
60 70 80 90 100
TLDCEDSDEQ DENDKLELTL DGTNDGRTIK LDPTYLAPGE KLVGYVLVKM
110 120 130 140 150
NDDPDQQNEP PNGHITSLSV MRTYRRMGIA ENLMRQALFA LREVHQAEYV
160 170 180 190 200
SLHVRQSNRA ALHLYRDTLA FEVLSIEKSY YQDGEDAYAM KKVLKLEELQ
210 220 230
ISNFTHRRLK ENEEKLEDDL ESDLLEDIIK QGVNDIIV
Length:238
Mass (Da):27,603
Last modified:February 1, 1995 - v2
Checksum:iAEB02BA5012D1137
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37I → T in AAA66323 (PubMed:3907857).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11621 Genomic DNA. Translation: AAA66323.1.
U10400 Genomic DNA. Translation: AAB68937.1.
AY557822 Genomic DNA. Translation: AAS56148.1.
BK006934 Genomic DNA. Translation: DAA06702.1.
PIRiS46783. TWBYA1.
RefSeqiNP_011877.1. NM_001179143.1.

Genome annotation databases

EnsemblFungiiYHR013C; YHR013C; YHR013C.
GeneIDi856404.
KEGGisce:YHR013C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11621 Genomic DNA. Translation: AAA66323.1.
U10400 Genomic DNA. Translation: AAB68937.1.
AY557822 Genomic DNA. Translation: AAS56148.1.
BK006934 Genomic DNA. Translation: DAA06702.1.
PIRiS46783. TWBYA1.
RefSeqiNP_011877.1. NM_001179143.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HNWX-ray2.80B1-238[»]
4HNXX-ray2.34B1-238[»]
4HNYX-ray2.25B/D1-238[»]
4XNHX-ray2.10B1-238[»]
4XPDX-ray2.81B1-238[»]
4Y49X-ray3.95B/H/N1-238[»]
ProteinModelPortaliP07347.
SMRiP07347.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36440. 320 interactors.
DIPiDIP-6788N.
IntActiP07347. 32 interactors.
MINTiMINT-617928.

Proteomic databases

MaxQBiP07347.
PRIDEiP07347.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR013C; YHR013C; YHR013C.
GeneIDi856404.
KEGGisce:YHR013C.

Organism-specific databases

EuPathDBiFungiDB:YHR013C.
SGDiS000001055. ARD1.

Phylogenomic databases

GeneTreeiENSGT00550000074803.
HOGENOMiHOG000078523.
InParanoidiP07347.
KOiK20791.
OMAiWPEASFV.
OrthoDBiEOG092C4MWD.

Enzyme and pathway databases

BioCyciYEAST:YHR013C-MONOMER.

Miscellaneous databases

PROiP07347.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 2 hits.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARD1_YEAST
AccessioniPrimary (citable) accession number: P07347
Secondary accession number(s): D3DKV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3310 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.