P07346 (ASPA_PSEFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate ammonia-lyase
Short name=Aspartase
EC=4.3.1.1

Gene names

Name:

aspA

Organism

Pseudomonas fluorescens

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate = fumarate + NH3.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Aspartase subfamily.

Ontologies

Keywords
Molecular function	Lyase
Gene Ontology (GO)
Biological process	aspartate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular function	aspartate ammonia-lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Aspartate ammonia-lyase		PRO_0000161344
Regions
	Region	148 – 150	3	Substrate binding By similarity
Sites
	Binding site	109	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07346 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: E7903CC4DA7C99C8	FASTA	478	51,508
	10 20 30 40 50 60 MISVMSSAAS FRTEKDLLGV LEVPAQAYYG IQTLRAVNNF RLSGVPISHY PKLVVGLAMV 70 80 90 100 110 120 KQAAADANRE LGQLSERKHA AISEACARLI RGDFHEEFVV DMIQGGAGTS TNMNANEVIA 130 140 150 160 170 180 NIALEAMGHQ KGEYQYLHPN NDVNMAQSTN DAYPTAIRLG LLLGHDALLA SLDSLIQAFA 190 200 210 220 230 240 AKGAEFSHVL KMGRTQLQDA VPMTLGQEFR AFATTLGEDL ARLKTLAPEL LTEVNLGGTA 250 260 270 280 290 300 IGTGINADPR YQALAVQRLA TISGQPLVPA ADLIEATSDM GAFVLFSGML KRTAVKLSKI 310 320 330 340 350 360 CNDLRLLSSG PRTGINEINL PARQPGSSIM PGKVNPVIPE AVNQVAFQVI GNDLALTMAA 370 380 390 400 410 420 EGGQLQLNVM EPLIAFKIFD SIRLLQRAMD MLREHCIVGI TANEARCREL VEHSIGLVTA 430 440 450 460 470 LNPYIGYENA TRIARIALES GRGVLELVRE EGLLDDAMLD DILRPENMIA PRLVPLKA

References

[1]	"Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens." Takagi J.S., Tokushige M., Shimura Y. J. Biochem. 100:697-705(1986) [PubMed: 3096982] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00100 Genomic DNA. Translation: BAA00062.1. X04441 Genomic DNA. Translation: CAA28037.1.
PIR	UFPSDF. A24874.
3D structure databases
ProteinModelPortal	P07346.
SMR	P07346. Positions 9-464.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004708. ApsA. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. IPR024083. L-Aspartase-like_N. IPR022761. Lyase1_N. [Graphical view]
Gene3D	G3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit.
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00839. AspA. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPA_PSEFL

Accession

Primary (citable) accession number: P07346

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	July 27, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program