P07345 (TRPB_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan synthase beta chain
EC=4.2.1.20

Gene names

Name:	trpB
Ordered Locus Names:	PA0036

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine. HAMAP MF_00133
Catalytic activity	L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H2O. HAMAP MF_00133
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00133
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5. HAMAP MF_00133
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Sequence similarities	Belongs to the TrpB family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro tryptophan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	Tryptophan synthase beta chain HAMAP MF_00133		PRO_0000098981
Amino acid modifications
	Modified residue	93	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	16	1	R → G in AAA88462. Ref.1
	Sequence conflict	123	1	V → L in AAA88462. Ref.1
	Sequence conflict	325	1	T → I in AAA88462. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P07345 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: F4D59601BBDFC016	FASTA	402	43,681
	10 20 30 40 50 60 MTSYRNGPDA KGLFGRFGGQ YVAETLMPLI LDLAREYEKA KDDPAFQEEL AYFQRDYVGR 70 80 90 100 110 120 PSPLYFAERL TEHCGGAKIY LKREELNHTG AHKINNCIGQ ILLARRMGKK RIIAETGAGM 130 140 150 160 170 180 HGVATATVAA RFGLQCVIYM GTTDIDRQQA NVFRMKLLGA EVIPVTAGTG TLKDAMNEAL 190 200 210 220 230 240 RDWVTNVDST FYLIGTVAGP HPYPAMVRDF QAVIGKETRE QLAEKEGRLP DSLVACIGGG 250 260 270 280 290 300 SNAMGLFHPF LDDAGVQIVG VEAAGHGIDT GKHAASLNGG VPGVLHGNRT FLLQDADGQI 310 320 330 340 350 360 IDAHSISAGL DYPGIGPEHA WLHDTGRVEY TSITDDEALE AFHTCCRLEG IIPALESSHA 370 380 390 400 LAEVFKRAPS LPKEHIMVVN LSGRGDKDMQ TVMHHMQQES KA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the genes for tryptophan synthase in Pseudomonas aeruginosa." Hadero A., Crawford I.P. Mol. Biol. Evol. 3:191-204(1986) [PubMed: 3127651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	Crawford I.P. Submitted (MAY-1987) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M15826 Genomic DNA. Translation: AAA88462.1. AE004091 Genomic DNA. Translation: AAG03426.1.
PIR	TSPSBA. A25355. H83640.
RefSeq	NP_248726.1. NC_002516.2.
3D structure databases
ProteinModelPortal	P07345.
SMR	P07345. Positions 14-393.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	879212.
GenomeReviews	Gene locus PA0036 in contig AE004091_GR.
KEGG	pae:PA0036.
PATRIC	19834268. VBIPseAer58763_0036.
Organism-specific databases
PseudoCAP	PA0036.
Phylogenomic databases
HOGENOM	HBG303148.
OMA	LKREDLC.
ProtClustDB	PRK04346.
Enzyme and pathway databases
BioCyc	PAER208964:PA0036-MONOMER.
Family and domain databases
HAMAP	MF_00133. Trp_synth_beta. [Tree]
InterPro	IPR001926. PyrdxlP-dep_enz_bsu. IPR006653. Trp_synth_b_CS. IPR006654. Trp_synth_beta. IPR023026. Trp_synth_beta/beta-like. [Graphical view]
KO	K01696.
PANTHER	PTHR10314:SF3. PTHR10314:SF3. 1 hit.
Pfam	PF00291. PALP. 1 hit. [Graphical view]
PIRSF	PIRSF001413. Trp_syn_beta. 1 hit.
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR00263. TrpB. 1 hit.
PROSITE	PS00168. TRP_SYNTHASE_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPB_PSEAE

Accession

Primary (citable) accession number: P07345

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	December 8, 2000
Last modified:	January 25, 2012
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program