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Protein

Tryptophan synthase beta chain

Gene

trpB

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.By similarity

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

Cofactori

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase component 1 (trpE), Anthranilate synthase component 2 (trpG)
  2. Anthranilate phosphoribosyltransferase (trpD)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (trpC)
  5. Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase beta chain (EC:4.2.1.20)
Gene namesi
Name:trpB
Ordered Locus Names:PA0036
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0036.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000989811 – 402Tryptophan synthase beta chainAdd BLAST402

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei93N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiP07345.
PRIDEiP07345.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.By similarity

Protein-protein interaction databases

STRINGi208964.PA0036.

Structurei

3D structure databases

ProteinModelPortaliP07345.
SMRiP07345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpB family.Curated

Phylogenomic databases

eggNOGiENOG4105CG0. Bacteria.
COG0133. LUCA.
HOGENOMiHOG000161710.
InParanoidiP07345.
KOiK01696.
OMAiEHSWLND.
PhylomeDBiP07345.

Family and domain databases

CDDicd06446. Trp-synth_B. 1 hit.
HAMAPiMF_00133. Trp_synth_beta. 1 hit.
InterProiIPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00263. trpB. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07345-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSYRNGPDA KGLFGRFGGQ YVAETLMPLI LDLAREYEKA KDDPAFQEEL
60 70 80 90 100
AYFQRDYVGR PSPLYFAERL TEHCGGAKIY LKREELNHTG AHKINNCIGQ
110 120 130 140 150
ILLARRMGKK RIIAETGAGM HGVATATVAA RFGLQCVIYM GTTDIDRQQA
160 170 180 190 200
NVFRMKLLGA EVIPVTAGTG TLKDAMNEAL RDWVTNVDST FYLIGTVAGP
210 220 230 240 250
HPYPAMVRDF QAVIGKETRE QLAEKEGRLP DSLVACIGGG SNAMGLFHPF
260 270 280 290 300
LDDAGVQIVG VEAAGHGIDT GKHAASLNGG VPGVLHGNRT FLLQDADGQI
310 320 330 340 350
IDAHSISAGL DYPGIGPEHA WLHDTGRVEY TSITDDEALE AFHTCCRLEG
360 370 380 390 400
IIPALESSHA LAEVFKRAPS LPKEHIMVVN LSGRGDKDMQ TVMHHMQQES

KA
Length:402
Mass (Da):43,681
Last modified:December 8, 2000 - v2
Checksum:iF4D59601BBDFC016
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16R → G in AAA88462 (PubMed:3127651).Curated1
Sequence conflicti123V → L in AAA88462 (PubMed:3127651).Curated1
Sequence conflicti325T → I in AAA88462 (PubMed:3127651).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15826 Genomic DNA. Translation: AAA88462.1.
AE004091 Genomic DNA. Translation: AAG03426.1.
PIRiA25355. TSPSBA.
H83640.
RefSeqiNP_248726.1. NC_002516.2.
WP_003097340.1. NZ_ASJY01000005.1.

Genome annotation databases

EnsemblBacteriaiAAG03426; AAG03426; PA0036.
GeneIDi879212.
KEGGipae:PA0036.
PATRICi19834268. VBIPseAer58763_0036.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15826 Genomic DNA. Translation: AAA88462.1.
AE004091 Genomic DNA. Translation: AAG03426.1.
PIRiA25355. TSPSBA.
H83640.
RefSeqiNP_248726.1. NC_002516.2.
WP_003097340.1. NZ_ASJY01000005.1.

3D structure databases

ProteinModelPortaliP07345.
SMRiP07345.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0036.

Proteomic databases

PaxDbiP07345.
PRIDEiP07345.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG03426; AAG03426; PA0036.
GeneIDi879212.
KEGGipae:PA0036.
PATRICi19834268. VBIPseAer58763_0036.

Organism-specific databases

PseudoCAPiPA0036.

Phylogenomic databases

eggNOGiENOG4105CG0. Bacteria.
COG0133. LUCA.
HOGENOMiHOG000161710.
InParanoidiP07345.
KOiK01696.
OMAiEHSWLND.
PhylomeDBiP07345.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.

Family and domain databases

CDDicd06446. Trp-synth_B. 1 hit.
HAMAPiMF_00133. Trp_synth_beta. 1 hit.
InterProiIPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00263. trpB. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPB_PSEAE
AccessioniPrimary (citable) accession number: P07345
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: December 8, 2000
Last modified: November 30, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.