P07344 (TRPA_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan synthase alpha chain
EC=4.2.1.20

Gene names

Name:	trpA
Ordered Locus Names:	PA0035

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. HAMAP MF_00131
Catalytic activity	L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H2O. HAMAP MF_00131
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5. HAMAP MF_00131
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Sequence similarities	Belongs to the TrpA family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	tryptophan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Tryptophan synthase alpha chain HAMAP MF_00131		PRO_0000098825
Sites
	Active site	49	1	Proton acceptor By similarity
	Active site	60	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	42	1	A → G in AAA88463. Ref.1
	Sequence conflict	73	1	D → E in AAA88463. Ref.1
	Sequence conflict	148	1	I → L in AAA88463. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P07344 [UniParc]. Last modified December 8, 2000. Version 3. Checksum: 6EB7052524A9BEAD	FASTA	268	28,488
	10 20 30 40 50 60 MSRLQTRFAQ LKQENRAALV TFVTAGDPDY ASSLEILKGL PAAGADVIEL GMPFTDPMAD 70 80 90 100 110 120 GPAIQLANIR ALDGGQTLAR TLQMVREFRS GDSETPLVLM GYFNPIHHYG VERFIAEAKE 130 140 150 160 170 180 VGVDGLIVVD LPPEHNEDLC HPAQAAGIDF IRLTTPTTGD QRLPTVLEGS SGFVYYVSVA 190 200 210 220 230 240 GVTGANAATL EHVEEAVARL RRHTDLPIGI GFGIRSAEHA AAVARLADGV VVGSALIDRI 250 260 AKARDNAQAV KDVLALCGEL AEGVRNAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the genes for tryptophan synthase in Pseudomonas aeruginosa." Hadero A., Crawford I.P. Mol. Biol. Evol. 3:191-204(1986) [PubMed: 3127651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	Crawford I.P., Eberly L. Submitted (MAR-1989) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M15826 Genomic DNA. Translation: AAA88463.1. AE004091 Genomic DNA. Translation: AAG03425.1.
PIR	TSPSAA. B25355. G83640.
RefSeq	NP_248725.1. NC_002516.2.
3D structure databases
ProteinModelPortal	P07344.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	879479.
GenomeReviews	Gene locus PA0035 in contig AE004091_GR.
KEGG	pae:PA0035.
PATRIC	19834266. VBIPseAer58763_0035.
Organism-specific databases
PseudoCAP	PA0035.
Phylogenomic databases
HOGENOM	HBG391690.
OMA	NPIYIYG.
ProtClustDB	PRK13111.
Enzyme and pathway databases
BioCyc	PAER208964:PA0035-MONOMER.
Family and domain databases
HAMAP	MF_00131. Trp_synth_alpha. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR011060. RibuloseP-bd_barrel. IPR018204. Trp_synthase_alpha_AS. IPR002028. Trp_synthase_suA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01695.
Pfam	PF00290. Trp_syntA. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00262. TrpA. 1 hit.
PROSITE	PS00167. TRP_SYNTHASE_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPA_PSEAE

Accession

Primary (citable) accession number: P07344

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	December 8, 2000
Last modified:	January 25, 2012
This is version 90 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program