P07343 (FUMC_BACSU) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 98.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Fumarate hydratase class II Short name=Fumarase C EC=4.2.1.2 | ||||||
| Gene names |
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| Organism | Bacillus subtilis | ||||||
| Taxonomic identifier | 1423 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 462 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Catalytic activity | (S)-malate = fumarate + H2O. HAMAP MF_00743 |
| Pathway | Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP MF_00743 |
| Subunit structure | Homotetramer. |
| Subcellular location | |
| Miscellaneous | There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity. HAMAP MF_00743 |
| Sequence similarities | Belongs to the class-II fumarase/aspartase family. Fumarase subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Tricarboxylic acid cycle |
| Cellular component | Cytoplasm |
| Molecular function | Lyase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycleInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro |
| Molecular function | fumarate hydratase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 462 | 462 | Fumarate hydratase class II HAMAP MF_00743 | PRO_0000161255 | |||||
Regions | |||||||||
| Region | 127 – 130 | 4 | B site By similarity | ||||||
| Region | 137 – 139 | 3 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Binding site | 99 | 1 | Substrate By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 66 | 1 | A → V in CAA25849. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Complete nucleotide sequence of the fumarase gene (citG) of Bacillus subtilis 168." Miles J.S., Guest J.R. Nucleic Acids Res. 13:131-140(1985) [PubMed: 3923430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168. |
| [2] | "The yvsA-yvqA (293 degrees - 289 degrees) region of the Bacillus subtilis chromosome containing genes involved in metal ion uptake and a putative sigma factor." Wipat A., Brignell C.S., Guy J.B., Rose M., Emmerson P.T., Harwood C.R. Microbiology 144:1593-1600(1998) [PubMed: 9639930] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168. |
| [3] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. Danchin A.Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
| [4] | "The nucleotide sequence of a spore germination gene (gerA) of Bacillus subtilis 168." Feavers I.M., Miles J.S., Moir A. Gene 38:95-102(1985) [PubMed: 2998952] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50. Strain: 168. |
| [5] | "Role of sigma H in expression of the fumarase gene (citG) in vegetative cells of Bacillus subtilis 168." Price V.A., Feavers I.M., Moir A. J. Bacteriol. 171:5933-5939(1989) [PubMed: 2509423] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50. Strain: 168. |
| [6] | "Sequencing of regions downstream of addA (98 degrees) and citG (289 degrees) in Bacillus subtilis." Medina N., Vannier F., Roche B., Autret S., Levine A., Seror S.J. Microbiology 143:3305-3308(1997) [PubMed: 9353931] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 224-462. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X01701 Genomic DNA. Translation: CAA25849.1. AJ223978 Genomic DNA. Translation: CAA11749.1. AL009126 Genomic DNA. Translation: CAB15294.1. M11918 Genomic DNA. Translation: AAA22464.1. Z93941 Genomic DNA. Translation: CAB07973.1. |
| PIR | UFBSC8. A23033. |
| RefSeq | NP_391184.1. NC_000964.3. |
3D structure databases | |
| ProteinModelPortal | P07343. |
| SMR | P07343. Positions 4-457. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P07343. 1 interaction. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBBACT00000000200; EBBACP00000000200; EBBACG00000000200. |
| GeneID | 938591. |
| GenomeReviews | Gene locus BSU33040 in contig AL009126_GR. |
| KEGG | bsu:BSU33040. |
| NMPDR | fig|224308.1.peg.3310. |
| PATRIC | 18978594. VBIBacSub10457_3460. |
Organism-specific databases | |
| GenoList | BSU33040. [Micado] |
Phylogenomic databases | |
| GeneTree | EBGT00070000031773. |
| HOGENOM | HBG284369. |
| OMA | RIEKDTM. |
| PhylomeDB | P07343. |
| ProtClustDB | PRK00485. |
Enzyme and pathway databases | |
| BioCyc | BSUB:BSU33040-MONOMER. |
Family and domain databases | |
| HAMAP | MF_00743. FumaraseC. [Tree] |
| InterPro | IPR003031. D_crystallin. IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. IPR024083. L-Aspartase-like_N. IPR022761. Lyase1_N. [Graphical view] |
| Gene3D | G3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit. |
| KO | K01679. |
| Pfam | PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view] |
| PRINTS | PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE. |
| SUPFAM | SSF48557. L-Aspartase-like. 1 hit. |
| TIGRFAMs | TIGR00979. FumC_II. 1 hit. |
| PROSITE | PS00163. FUMARATE_LYASES. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | FUMC_BACSU | ||||||||
| Accession | Primary (citable) accession number: P07343 Secondary accession number(s): O32194 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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