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P07343 (FUMC_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fumarate hydratase class II
Short name=Fumarase C
EC=4.2.1.2
Gene names
Name:fumC
Synonyms:citG
Ordered Locus Names:BSU33040
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length462 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(S)-malate = fumarate + H2O. HAMAP MF_00743

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP MF_00743

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm HAMAP MF_00743.

Miscellaneous

There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity. HAMAP MF_00743

Sequence similarities

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentCytoplasm
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processfumarate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componenttricarboxylic acid cycle enzyme complex

Inferred from electronic annotation. Source: InterPro

   Molecular functionfumarate hydratase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 462462Fumarate hydratase class II HAMAP MF_00743
PRO_0000161255

Regions

Region127 – 1304B site By similarity
Region137 – 1393Substrate binding By similarity

Sites

Binding site991Substrate By similarity

Experimental info

Sequence conflict661A → V in CAA25849. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P07343 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 8D797D18FEA82C06

FASTA46250,532
        10         20         30         40         50         60 
MEYRIERDTM GEVKVPADKF WGAQTQRSKE NFKIGSEKMP MRVVKAFAIL KRSTALANKR 

        70         80         90        100        110        120 
LGNLDAEKAE AIAAVCDDVL KGKYDDNFPL VVWQTGSGTQ SNMNMNEVVA NRATALLKEK 

       130        140        150        160        170        180 
NSDQTIHPND DVNRSQSSND TFPTAMHVAA VLAVYEQLVP ALDQLRNTLD EKAKAYNDIV 

       190        200        210        220        230        240 
KIGRTHLQDA TPLTLGQEIS GWVHMLDRSK EMILEATDKM RALAIGGTAV GTGINAHPEF 

       250        260        270        280        290        300 
GELVSEEITK LTGQTFSSSP NKFHALTSHD EITYAHGALK ALAADLMKIA NDVRWLASGP 

       310        320        330        340        350        360 
RCGIGEIVIP ENEPGSSIMP GKVNPTQSEA LTMIAAQIMG NDATIGFAAS QGNFELNVFK 

       370        380        390        400        410        420 
PVIIYNFLQS VQLLSDGMNS FHDKCAVGIE PNKETIQENL SNSLMLVTAL NPHIGYENAA 

       430        440        450        460 
KIAKLAHKEG LTLKEAALKL ELLTEEQFNE MVKPEDMVKP KA

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of the fumarase gene (citG) of Bacillus subtilis 168."
Miles J.S., Guest J.R.
Nucleic Acids Res. 13:131-140(1985) [PubMed: 3923430] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The yvsA-yvqA (293 degrees - 289 degrees) region of the Bacillus subtilis chromosome containing genes involved in metal ion uptake and a putative sigma factor."
Wipat A., Brignell C.S., Guy J.B., Rose M., Emmerson P.T., Harwood C.R.
Microbiology 144:1593-1600(1998) [PubMed: 9639930] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"The nucleotide sequence of a spore germination gene (gerA) of Bacillus subtilis 168."
Feavers I.M., Miles J.S., Moir A.
Gene 38:95-102(1985) [PubMed: 2998952] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50.
Strain: 168.
[5]"Role of sigma H in expression of the fumarase gene (citG) in vegetative cells of Bacillus subtilis 168."
Price V.A., Feavers I.M., Moir A.
J. Bacteriol. 171:5933-5939(1989) [PubMed: 2509423] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50.
Strain: 168.
[6]"Sequencing of regions downstream of addA (98 degrees) and citG (289 degrees) in Bacillus subtilis."
Medina N., Vannier F., Roche B., Autret S., Levine A., Seror S.J.
Microbiology 143:3305-3308(1997) [PubMed: 9353931] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 224-462.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X01701 Genomic DNA. Translation: CAA25849.1.
AJ223978 Genomic DNA. Translation: CAA11749.1.
AL009126 Genomic DNA. Translation: CAB15294.1.
M11918 Genomic DNA. Translation: AAA22464.1.
Z93941 Genomic DNA. Translation: CAB07973.1.
PIRUFBSC8. A23033.
RefSeqNP_391184.1. NC_000964.3.

3D structure databases

ProteinModelPortalP07343.
SMRP07343. Positions 4-457.
ModBaseSearch...

Protein-protein interaction databases

IntActP07343. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000000200; EBBACP00000000200; EBBACG00000000200.
GeneID938591.
GenomeReviewsGene locus BSU33040 in contig AL009126_GR.
KEGGbsu:BSU33040.
NMPDRfig|224308.1.peg.3310.
PATRIC18978594. VBIBacSub10457_3460.

Organism-specific databases

GenoListBSU33040. [Micado]

Phylogenomic databases

GeneTreeEBGT00070000031773.
HOGENOMHBG284369.
OMARIEKDTM.
PhylomeDBP07343.
ProtClustDBPRK00485.

Enzyme and pathway databases

BioCycBSUB:BSU33040-MONOMER.

Family and domain databases

HAMAPMF_00743. FumaraseC.
[Tree]
InterProIPR003031. D_crystallin.
IPR005677. Fum_hydII.
IPR018951. Fumarase_C_C.
IPR000362. Fumarate_lyase.
IPR020557. Fumarate_lyase_CS.
IPR008948. L-Aspartase-like.
IPR024083. L-Aspartase-like_N.
IPR022761. Lyase1_N.
[Graphical view]
Gene3DG3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit.
KOK01679.
PfamPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSPR00145. ARGSUCLYASE.
PR00149. FUMRATELYASE.
SUPFAMSSF48557. L-Aspartase-like. 1 hit.
TIGRFAMsTIGR00979. FumC_II. 1 hit.
PROSITEPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFUMC_BACSU
AccessionPrimary (citable) accession number: P07343
Secondary accession number(s): O32194
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: May 30, 2000
Last modified: January 25, 2012
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents