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Protein

Sodium/potassium-transporting ATPase subunit beta-1

Gene

Atp1b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.1 Publication
Involved in cell adhesion and establishing epithelial cell polarity.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

Potassium, Sodium

Enzyme and pathway databases

ReactomeiR-RNO-210991. Basigin interactions.
R-RNO-5578775. Ion homeostasis.
R-RNO-936837. Ion transport by P-type ATPases.
SABIO-RKP07340.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit beta-1
Alternative name(s):
Sodium/potassium-dependent ATPase subunit beta-1
Gene namesi
Name:Atp1b1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi2170. Atp1b1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3434CytoplasmicSequence analysisAdd
BLAST
Transmembranei35 – 6228Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini63 – 304242ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • basolateral plasma membrane Source: RGD
  • caveola Source: RGD
  • extracellular exosome Source: Ensembl
  • intercalated disc Source: Ensembl
  • intracellular Source: Ensembl
  • myelin sheath Source: Ensembl
  • sarcolemma Source: Ensembl
  • sodium:potassium-exchanging ATPase complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304Sodium/potassium-transporting ATPase subunit beta-1PRO_0000219100Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111PhosphoserineCombined sources
Modified residuei101 – 1011PhosphotyrosineBy similarity
Disulfide bondi126 ↔ 149By similarity
Glycosylationi158 – 1581N-linked (GlcNAc...)By similarity
Disulfide bondi159 ↔ 175By similarity
Glycosylationi193 – 1931N-linked (GlcNAc...)By similarity
Disulfide bondi214 ↔ 277By similarity
Glycosylationi266 – 2661N-linked (GlcNAc...)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP07340.
PRIDEiP07340.

PTM databases

iPTMnetiP07340.
PhosphoSiteiP07340.
SwissPalmiP07340.
UniCarbKBiP07340.

Expressioni

Gene expression databases

ExpressionAtlasiP07340. baseline and differential.
GenevisibleiP07340. RN.

Interactioni

Subunit structurei

Composed of three subunits: alpha (catalytic), beta and gamma. Interacts with NKAIN1, NKAIN2 and NKAIN4 (By similarity). Interacts with MLC1. Part of a complex containing MLC1, TRPV4, AQP4 and HEPACAM. Interacts with KIRREL3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi247679. 2 interactions.
IntActiP07340. 3 interactions.
MINTiMINT-3089264.
STRINGi10116.ENSRNOP00000003932.

Structurei

3D structure databases

ProteinModelPortaliP07340.
SMRiP07340. Positions 28-73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni191 – 304114immunoglobulin-likeBy similarityAdd
BLAST

Domaini

The C-terminal lobe folds into an immunoglobulin-like domain and mediates cell adhesion properties.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3927. Eukaryota.
ENOG411150A. LUCA.
GeneTreeiENSGT00550000074530.
HOGENOMiHOG000039248.
HOVERGENiHBG050603.
InParanoidiP07340.
KOiK01540.
OMAiRGEFNNE.
OrthoDBiEOG7M6D85.
PhylomeDBiP07340.
TreeFamiTF314618.

Family and domain databases

InterProiIPR000402. Na/K_ATPase_sub_beta.
IPR015565. Na/K_ATPase_sub_beta_chordates.
[Graphical view]
PANTHERiPTHR11523. PTHR11523. 1 hit.
PTHR11523:SF10. PTHR11523:SF10. 1 hit.
PfamiPF00287. Na_K-ATPase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITEiPS00390. ATPASE_NA_K_BETA_1. 1 hit.
PS00391. ATPASE_NA_K_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07340-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGKAKEEG SWKKFIWNSE KKEFLGRTGG SWFKILLFYV IFYGCLAGIF
60 70 80 90 100
IGTIQVMLLT ISELKPTYQD RVAPPGLTQI PQIQKTEISF RPNDPKSYEA
110 120 130 140 150
YVLNIIRFLE KYKDSAQKDD MIFEDCGSMP SEPKERGEFN HERGERKVCR
160 170 180 190 200
FKLDWLGNCS GLNDESYGYK EGKPCIIIKL NRVLGFKPKP PKNESLETYP
210 220 230 240 250
LTMKYNPNVL PVQCTGKRDE DKDKVGNIEY FGMGGFYGFP LQYYPYYGKL
260 270 280 290 300
LQPKYLQPLL AVQFTNLTLD TEIRIECKAY GENIGYSEKD RFQGRFDVKI

EVKS
Length:304
Mass (Da):35,202
Last modified:April 1, 1988 - v1
Checksum:i86C3120F463B7B2B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti183 – 1831V → M in AAA40780 (PubMed:3031033).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14137 mRNA. Translation: AAA40781.1.
J02701 mRNA. Translation: AAA40780.1.
BC078902 mRNA. Translation: AAH78902.1.
X63375 Genomic DNA. No translation available.
PIRiA25082. PWRTNB.
RefSeqiNP_037245.2. NM_013113.2.
UniGeneiRn.8925.

Genome annotation databases

EnsembliENSRNOT00000003932; ENSRNOP00000003932; ENSRNOG00000002934.
GeneIDi25650.
KEGGirno:25650.
UCSCiRGD:2170. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14137 mRNA. Translation: AAA40781.1.
J02701 mRNA. Translation: AAA40780.1.
BC078902 mRNA. Translation: AAH78902.1.
X63375 Genomic DNA. No translation available.
PIRiA25082. PWRTNB.
RefSeqiNP_037245.2. NM_013113.2.
UniGeneiRn.8925.

3D structure databases

ProteinModelPortaliP07340.
SMRiP07340. Positions 28-73.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247679. 2 interactions.
IntActiP07340. 3 interactions.
MINTiMINT-3089264.
STRINGi10116.ENSRNOP00000003932.

PTM databases

iPTMnetiP07340.
PhosphoSiteiP07340.
SwissPalmiP07340.
UniCarbKBiP07340.

Proteomic databases

PaxDbiP07340.
PRIDEiP07340.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003932; ENSRNOP00000003932; ENSRNOG00000002934.
GeneIDi25650.
KEGGirno:25650.
UCSCiRGD:2170. rat.

Organism-specific databases

CTDi481.
RGDi2170. Atp1b1.

Phylogenomic databases

eggNOGiKOG3927. Eukaryota.
ENOG411150A. LUCA.
GeneTreeiENSGT00550000074530.
HOGENOMiHOG000039248.
HOVERGENiHBG050603.
InParanoidiP07340.
KOiK01540.
OMAiRGEFNNE.
OrthoDBiEOG7M6D85.
PhylomeDBiP07340.
TreeFamiTF314618.

Enzyme and pathway databases

ReactomeiR-RNO-210991. Basigin interactions.
R-RNO-5578775. Ion homeostasis.
R-RNO-936837. Ion transport by P-type ATPases.
SABIO-RKP07340.

Miscellaneous databases

NextBioi607519.
PROiP07340.

Gene expression databases

ExpressionAtlasiP07340. baseline and differential.
GenevisibleiP07340. RN.

Family and domain databases

InterProiIPR000402. Na/K_ATPase_sub_beta.
IPR015565. Na/K_ATPase_sub_beta_chordates.
[Graphical view]
PANTHERiPTHR11523. PTHR11523. 1 hit.
PTHR11523:SF10. PTHR11523:SF10. 1 hit.
PfamiPF00287. Na_K-ATPase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITEiPS00390. ATPASE_NA_K_BETA_1. 1 hit.
PS00391. ATPASE_NA_K_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rat-brain Na,K-ATPase beta-chain gene: primary structure, tissue-specific expression, and amplification in ouabain-resistant HeLa C+ cells."
    Mercer R.W., Schneider J.W., Savitz A., Emanuel J.R., Benz E.J. Jr., Levenson R.
    Mol. Cell. Biol. 6:3884-3890(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Multiple mRNAs from rat kidney and brain encode a single Na+,K+-ATPase beta subunit protein."
    Young R.M., Shull G.E., Lingrel J.B.
    J. Biol. Chem. 262:4905-4910(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "Characterization of the 5' flanking region of the rat Na+/K(+)-ATPase beta 1 subunit gene."
    Liu B., Gick G.
    Biochim. Biophys. Acta 1130:336-338(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
  5. Lubec G., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 15-21; 72-85; 97-107; 205-217 AND 279-291, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  6. "Identification of the amino acid region involved in the intercellular interaction between the beta1 subunits of Na+/K+ -ATPase."
    Tokhtaeva E., Sachs G., Sun H., Dada L.A., Sznajder J.I., Vagin O.
    J. Cell Sci. 125:1605-1616(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN ADHESION.
  7. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAT1B1_RAT
AccessioniPrimary (citable) accession number: P07340
Secondary accession number(s): Q63062
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: May 11, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.