P07340 (AT1B1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium/potassium-transporting ATPase subunit beta-1

Alternative name(s):
Sodium/potassium-dependent ATPase subunit beta-1

Gene names

Name:

Atp1b1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Subunit structure	Composed of three subunits: alpha (catalytic), beta and gamma. Interacts with NKAIN1, NKAIN2 and NKAIN4 By similarity.
Subcellular location	Membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the X(+)/potassium ATPases subunit beta family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Potassium Sodium
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro response to hypoxia Inferred from direct assay. Source: RGD
Cellular component	apical plasma membrane Inferred from direct assay. Source: RGD basolateral plasma membrane Inferred from direct assay. Source: RGD caveola Inferred from direct assay. Source: RGD sodium:potassium-exchanging ATPase complex Inferred from direct assay. Source: RGD
Molecular function	sodium:potassium-exchanging ATPase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Sodium/potassium-transporting ATPase subunit beta-1		PRO_0000219100
Regions
	Topological domain	1 – 34	34	Cytoplasmic Potential
	Transmembrane	35 – 62	28	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	63 – 304	242	Extracellular Potential
Amino acid modifications
	Modified residue	101	1	Phosphotyrosine By similarity
	Glycosylation	158	1	N-linked (GlcNAc...) By similarity
	Glycosylation	193	1	N-linked (GlcNAc...) By similarity
	Glycosylation	266	1	N-linked (GlcNAc...) By similarity
	Disulfide bond	126 ↔ 149		By similarity
	Disulfide bond	159 ↔ 175		By similarity
	Disulfide bond	214 ↔ 277		By similarity
Experimental info
	Sequence conflict	183	1	V → M in AAA40780. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P07340 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 86C3120F463B7B2B	FASTA	304	35,202
	10 20 30 40 50 60 MARGKAKEEG SWKKFIWNSE KKEFLGRTGG SWFKILLFYV IFYGCLAGIF IGTIQVMLLT 70 80 90 100 110 120 ISELKPTYQD RVAPPGLTQI PQIQKTEISF RPNDPKSYEA YVLNIIRFLE KYKDSAQKDD 130 140 150 160 170 180 MIFEDCGSMP SEPKERGEFN HERGERKVCR FKLDWLGNCS GLNDESYGYK EGKPCIIIKL 190 200 210 220 230 240 NRVLGFKPKP PKNESLETYP LTMKYNPNVL PVQCTGKRDE DKDKVGNIEY FGMGGFYGFP 250 260 270 280 290 300 LQYYPYYGKL LQPKYLQPLL AVQFTNLTLD TEIRIECKAY GENIGYSEKD RFQGRFDVKI EVKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rat-brain Na,K-ATPase beta-chain gene: primary structure, tissue-specific expression, and amplification in ouabain-resistant HeLa C+ cells." Mercer R.W., Schneider J.W., Savitz A., Emanuel J.R., Benz E.J. Jr., Levenson R. Mol. Cell. Biol. 6:3884-3890(1986) [PubMed: 3025616] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Multiple mRNAs from rat kidney and brain encode a single Na+,K+-ATPase beta subunit protein." Young R.M., Shull G.E., Lingrel J.B. J. Biol. Chem. 262:4905-4910(1987) [PubMed: 3031033] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"Characterization of the 5' flanking region of the rat Na+/K(+)-ATPase beta 1 subunit gene." Liu B., Gick G. Biochim. Biophys. Acta 1130:336-338(1992) [PubMed: 1314096] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
[5]	Lubec G., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 15-21; 72-85; 97-107; 205-217 AND 279-291, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M14137 mRNA. Translation: AAA40781.1. J02701 mRNA. Translation: AAA40780.1. BC078902 mRNA. Translation: AAH78902.1. X63375 Genomic DNA. No translation available.
IPI	IPI00339124.
PIR	PWRTNB. A25082.
RefSeq	NP_037245.2. NM_013113.2.
UniGene	Rn.8925.
3D structure databases
ProteinModelPortal	P07340.
SMR	P07340. Positions 28-73.
ModBase	Search...
Protein-protein interaction databases
IntAct	P07340. 1 interaction.
MINT	MINT-3089264.
STRING	P07340.
PTM databases
PhosphoSite	P07340.
Proteomic databases
PRIDE	P07340.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000003932; ENSRNOP00000003932; ENSRNOG00000002934.
GeneID	25650.
KEGG	rno:25650.
UCSC	NM_013113. rat.
Organism-specific databases
CTD	481.
RGD	2170. Atp1b1.
Phylogenomic databases
eggNOG	roNOG16210.
HOVERGEN	HBG050603.
InParanoid	P07340.
OrthoDB	EOG4M3998.
Gene expression databases
ArrayExpress	P07340.
Genevestigator	P07340.
GermOnline	ENSRNOG00000002934. Rattus norvegicus.
Family and domain databases
InterPro	IPR000402. ATPase_P-typ_cation-exchng_bsu. IPR015565. ATPase_P-typ_Na/K-dep_b1su. [Graphical view]
KO	K01540.
PANTHER	PTHR11523. ATPase_H_Na/K_b. 1 hit. PTHR11523:SF10. Na/K_ATPaseBeta1. 1 hit.
Pfam	PF00287. Na_K-ATPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITE	PS00390. ATPASE_NA_K_BETA_1. 1 hit. PS00391. ATPASE_NA_K_BETA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	607519.

Entry information

Entry name

AT1B1_RAT

Accession

Primary (citable) accession number: P07340
Secondary accession number(s): Q63062

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program