Reviewed, UniProtKB/Swiss-Prot P07337 (BGLS_KLUMA)

Names and origin

Protein names	Recommended name:     Beta-glucosidase     EC=3.2.1.21 Alternative name(s):     Gentiobiase     Cellobiase     Beta-D-glucoside glucohydrolase
Organism	Kluyveromyces marxianus (Yeast) (Candida kefyr)
Taxonomic identifier	4911 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	845 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Pathway	Glycan metabolism; cellulose degradation.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?
	Chain	? – 845		Beta-glucosidase		PRO_0000011778
Sites
	Active site	225	1	By similarity
Amino acid modifications
	Glycosylation	66	1	N-linked (GlcNAc...) Potential
	Glycosylation	304	1	N-linked (GlcNAc...) Potential
	Glycosylation	438	1	N-linked (GlcNAc...) Potential
	Glycosylation	621	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P07337-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 78D4C1550D5992B5	FASTA	845	93,917
	10 20 30 40 50 60 MSKFDVEQLL SELNQDEKIS LLSAVDFWHT KKIERLGIPA VRVSDGPNGI RGTKFFDGVP 70 80 90 100 110 120 SGCFPNGTGL ASTFDRDLLE TAGKLMAKES IAKNAAVILG PTTNMQRGPL GGRGFESFSE 130 140 150 160 170 180 DPYLAGMATS SVVKGMQGEG IAATVKHFVC NDLEDQRFSS NSIVSERALR EIYLEPFRLA 190 200 210 220 230 240 VKHANPVCIM TAYNKVNGDH CSQSKKLLID ILRDEWKWDG MLMSDWFGTY TTAAAIKNGL 250 260 270 280 290 300 DIEFPGPTRW RTRALVSHSL NSREQITTED VDDRVRQVLK MIKFVVDNLE KTGIVENGPE 310 320 330 340 350 360 STSNNTKETS DLLREIAADS IVLLKNKNNY LTSKERRQYH VIGPNAKAKT SSGGGSASMN 370 380 390 400 410 420 SYYVVSPYEG IVNKLGKEVD YTVGAYSHKS IGGLAESSLI DAAKPADAEN AGLIAKFYSN 430 440 450 460 470 480 PVEERSEDEE PFHVTKVNRS NVHLFDFKHE KVDPKNPYFF VTLTGQYVPQ EDGDYIFSLQ 490 500 510 520 530 540 VYGSGLFYLN DELIIDQKHN QERGSFCFGA GTKERTKKLT LKKGQVYNVR VEYGSGPTSG 550 560 570 580 590 600 LVGEFGAGGF QAGVIKAIDD DEEIRNAAEL AAKHDKAVLI IGLNGEWETE GYDRENMDLP 610 620 630 640 650 660 KRTNELVRAV LKANPNTVIV NQSGTPVEFP WLEEANALVQ AWYGGNELGN AIADVLYGDV 670 680 690 700 710 720 VPNGKLSLSW PFKLQDNPAF LNFKTEFGRV VYGEDIFVGY RYYEKLQRKV AFPFGYGLSY 730 740 750 760 770 780 TTFELDISDF KVTDDKIDIS VDVKNTGDKF AGSEVVQVYF SALNSKVSRP VKELKGFEKV 790 800 810 820 830 840 HLEPGEKKTV NIELELKDAI SYFNEELGKW HVEAGEYLVS VGTSSDDILS VKEFKVEKDL YWKGL

References

[1]

"Sequence and transcription of the beta-glucosidase gene of Kluyveromyces fragilis cloned in Saccharomyces cerevisiae." Raynal A., Gerbaud C., Francingues M.C., Guerineau M. Curr. Genet. 12:175-184(1987) [PubMed: 2835179] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 12424 / NRRL Y-610.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X05918 Genomic DNA. Translation: CAA29353.1.
PIR	GLVK. A29148.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH3. Glycoside Hydrolase Family 3.
Enzyme and pathway databases
BRENDA	3.2.1.21. 97088.
Family and domain databases
InterPro	IPR019800. Glyco_hydro_3_AS. IPR002772. Glyco_hydro_3_C. IPR001764. Glyco_hydro_3_N. IPR011658. PA14. [Graphical view]
Gene3D	G3DSA:3.20.20.300. Glyco_hydro_3_N. 1 hit.
Pfam	PF00933. Glyco_hydro_3. 1 hit. PF01915. Glyco_hydro_3_C. 1 hit. PF07691. PA14. 1 hit. [Graphical view]
PRINTS	PR00133. GLHYDRLASE3.
SMART	SM00758. PA14. 1 hit. [Graphical view]
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGLS_KLUMA

Accession

Primary (citable) accession number: P07337

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)