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Protein

Creatine kinase B-type

Gene

Ckb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei130 – 1301ATPPROSITE-ProRule annotation
Binding sitei132 – 1321ATPPROSITE-ProRule annotation
Binding sitei191 – 1911ATPPROSITE-ProRule annotation
Binding sitei232 – 2321SubstrateBy similarity
Binding sitei236 – 2361ATPPROSITE-ProRule annotation
Binding sitei285 – 2851SubstrateBy similarity
Binding sitei292 – 2921ATPPROSITE-ProRule annotation
Binding sitei320 – 3201ATPPROSITE-ProRule annotation
Binding sitei335 – 3351ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi128 – 1325ATPPROSITE-ProRule annotation
Nucleotide bindingi320 – 3256ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. creatine kinase activity Source: RGD

GO - Biological processi

  1. brain development Source: RGD
  2. cellular chloride ion homeostasis Source: RGD
  3. phosphocreatine metabolic process Source: RGD
  4. substantia nigra development Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_212547. Creatine metabolism.

Protein family/group databases

MoonProtiP07335.

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase B-type (EC:2.7.3.2)
Alternative name(s):
B-CK
Creatine kinase B chain
Gene namesi
Name:Ckb
Synonyms:Ckbb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 6

Organism-specific databases

RGDi2357. Ckb.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. mitochondrion Source: Ensembl
  3. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 381380Creatine kinase B-typePRO_0000211970Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei4 – 41PhosphoserineBy similarity
Modified residuei35 – 351PhosphothreonineBy similarity
Modified residuei125 – 1251PhosphotyrosineBy similarity
Modified residuei199 – 1991PhosphoserineBy similarity
Modified residuei269 – 2691Nitrated tyrosineBy similarity

Keywords - PTMi

Nitration, Phosphoprotein

Proteomic databases

PaxDbiP07335.
PRIDEiP07335.

2D gel databases

World-2DPAGE0004:P07335.

PTM databases

PhosphoSiteiP07335.

Expressioni

Tissue specificityi

In the kidney localized primarily in the outer medulla in the thick ascending limb and distal convoluted tubule.1 Publication

Gene expression databases

GenevestigatoriP07335.

Interactioni

Subunit structurei

Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

Protein-protein interaction databases

BioGridi246448. 1 interaction.
IntActiP07335. 1 interaction.
MINTiMINT-4586357.

Structurei

3D structure databases

ProteinModelPortaliP07335.
SMRiP07335. Positions 2-381.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 9888Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini125 – 367243Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG3869.
GeneTreeiENSGT00550000074561.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP07335.
KOiK00933.
OMAiQCLSDVR.
OrthoDBiEOG7XM2XW.
PhylomeDBiP07335.
TreeFamiTF314214.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07335-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPFSNSHNTQ KLRFPAEDEF PDLSSHNNHM AKVLTPELYA ELRAKCTPSG
60 70 80 90 100
FTLDDAIQTG VDNPGHPYIM TVGAVAGDEE SYDVFKDLFD PIIEDRHGGY
110 120 130 140 150
QPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE
160 170 180 190 200
RRAIEKLAVE ALSSLDGDLS GRYYALKSMT EAEQQQLIDD HFLFDKPVSP
210 220 230 240 250
LLLASGMARD WPDARGIWHN DNKTFLVWIN EEDHLRVISM QKGGNMKEVF
260 270 280 290 300
TRFCTGLTQI ETLFKSKNYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP
310 320 330 340 350
HLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV
360 370 380
QMVVDGVKLL IEMEQRLEQG QPIDDLMPAQ K
Length:381
Mass (Da):42,725
Last modified:January 9, 2007 - v2
Checksum:iA8C8A09F6FF1A4D1
GO

Sequence cautioni

The sequence AAH87656.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181D → A in AAA40931. (PubMed:2284002)Curated
Sequence conflicti18 – 181D → A in AAA40933. (PubMed:2284002)Curated
Sequence conflicti183 – 1842EQ → DE in AAA40930. (PubMed:3005113)Curated
Sequence conflicti183 – 1842EQ → DE in AAA40932. (PubMed:2838389)Curated
Sequence conflicti206 – 2061G → A in AAA40932. (PubMed:2838389)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14400 mRNA. Translation: AAA40930.1.
M18668 Genomic DNA. Translation: AAA40932.1.
M57664 mRNA. Translation: AAA40933.1.
M57665 Genomic DNA. Translation: AAA40931.1.
BC065307 mRNA. Translation: AAH65307.2.
BC070955 mRNA. Translation: AAH70955.2.
BC087656 mRNA. Translation: AAH87656.1. Different initiation.
BC099814 mRNA. Translation: AAH99814.2.
BC127477 mRNA. Translation: AAI27478.2.
PIRiA23980. KIRTCB.
RefSeqiNP_036661.3. NM_012529.3.
UniGeneiRn.1472.

Genome annotation databases

EnsembliENSRNOT00000015122; ENSRNOP00000015122; ENSRNOG00000010872.
GeneIDi24264.
KEGGirno:24264.
UCSCiRGD:2357. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14400 mRNA. Translation: AAA40930.1.
M18668 Genomic DNA. Translation: AAA40932.1.
M57664 mRNA. Translation: AAA40933.1.
M57665 Genomic DNA. Translation: AAA40931.1.
BC065307 mRNA. Translation: AAH65307.2.
BC070955 mRNA. Translation: AAH70955.2.
BC087656 mRNA. Translation: AAH87656.1. Different initiation.
BC099814 mRNA. Translation: AAH99814.2.
BC127477 mRNA. Translation: AAI27478.2.
PIRiA23980. KIRTCB.
RefSeqiNP_036661.3. NM_012529.3.
UniGeneiRn.1472.

3D structure databases

ProteinModelPortaliP07335.
SMRiP07335. Positions 2-381.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246448. 1 interaction.
IntActiP07335. 1 interaction.
MINTiMINT-4586357.

Chemistry

ChEMBLiCHEMBL2176812.

Protein family/group databases

MoonProtiP07335.

PTM databases

PhosphoSiteiP07335.

2D gel databases

World-2DPAGE0004:P07335.

Proteomic databases

PaxDbiP07335.
PRIDEiP07335.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015122; ENSRNOP00000015122; ENSRNOG00000010872.
GeneIDi24264.
KEGGirno:24264.
UCSCiRGD:2357. rat.

Organism-specific databases

CTDi1152.
RGDi2357. Ckb.

Phylogenomic databases

eggNOGiCOG3869.
GeneTreeiENSGT00550000074561.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP07335.
KOiK00933.
OMAiQCLSDVR.
OrthoDBiEOG7XM2XW.
PhylomeDBiP07335.
TreeFamiTF314214.

Enzyme and pathway databases

ReactomeiREACT_212547. Creatine metabolism.

Miscellaneous databases

NextBioi602815.

Gene expression databases

GenevestigatoriP07335.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of a rat brain creatine kinase-beta-galactosidase fusion protein in Escherichia coli and derivation of the complete amino acid sequence of rat brain creatine kinase."
    Benfield P.A., Henderson L., Pearson M.L.
    Gene 39:263-267(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation of four rat creatine kinase genes and identification of multiple potential promoter sequences within the rat brain creatine kinase promoter region."
    Benfield P.A., Graf D., Korolkoff P.N., Hobson G., Pearson M.L.
    Gene 63:227-243(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Estrogen regulation of creatine kinase-B in the rat uterus."
    Pentecost B.T., Mattheiss L., Dickerman H.W., Kumar S.A.
    Mol. Endocrinol. 4:1000-1010(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Tissue: Uterus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain, Embryonic brain, Heart and Prostate.
  5. Lubec G., Afjehi-Sadat L., Diao W., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 33-43; 87-96; 108-130; 138-148; 157-172; 224-236; 253-265 AND 320-341, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.
  6. "The brain isoform of a key ATP-regulating enzyme, creatine kinase, is a phosphoprotein."
    Mahadevan L.C., Whatley S.A., Leung T.K.C., Lim L.
    Biochem. J. 222:139-144(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
  7. "Compartmentation of multiple forms of creatine kinase in the distal nephron of the rat kidney."
    Friedman D.L., Perryman M.B.
    J. Biol. Chem. 266:22404-22410(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiKCRB_RAT
AccessioniPrimary (citable) accession number: P07335
Secondary accession number(s): A0JPK7
, Q499P7, Q5PPJ5, Q6IRE0, Q6P139
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 9, 2007
Last modified: January 7, 2015
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.