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Protein

Creatine kinase B-type

Gene

Ckb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei130ATPPROSITE-ProRule annotation1
Binding sitei132ATPPROSITE-ProRule annotation1
Binding sitei191ATPPROSITE-ProRule annotation1
Binding sitei232SubstrateBy similarity1
Binding sitei236ATPPROSITE-ProRule annotation1
Binding sitei285SubstrateBy similarity1
Binding sitei292ATPPROSITE-ProRule annotation1
Binding sitei320ATPPROSITE-ProRule annotation1
Binding sitei335ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi128 – 132ATPPROSITE-ProRule annotation5
Nucleotide bindingi320 – 325ATPPROSITE-ProRule annotation6

GO - Molecular functioni

GO - Biological processi

  • brain development Source: RGD
  • cellular chloride ion homeostasis Source: RGD
  • cerebellum development Source: RGD
  • substantia nigra development Source: RGD

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-71288 Creatine metabolism

Protein family/group databases

MoonProtiP07335

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase B-type (EC:2.7.3.2)
Alternative name(s):
B-CK
Creatine kinase B chain
Creatine phosphokinase M-type
Short name:
CPK-B
Gene namesi
Name:Ckb
Synonyms:Ckbb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi2357 Ckb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176812

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002119701 – 381Creatine kinase B-typeAdd BLAST381

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineBy similarity1
Modified residuei35PhosphothreonineCombined sources1
Modified residuei125PhosphotyrosineBy similarity1
Modified residuei199PhosphoserineBy similarity1
Modified residuei269Nitrated tyrosineBy similarity1
Modified residuei309PhosphoserineCombined sources1
Modified residuei322PhosphothreonineBy similarity1

Keywords - PTMi

Nitration, Phosphoprotein

Proteomic databases

PaxDbiP07335
PRIDEiP07335

2D gel databases

World-2DPAGEi0004:P07335

PTM databases

CarbonylDBiP07335
iPTMnetiP07335
PhosphoSitePlusiP07335

Expressioni

Tissue specificityi

In the kidney localized primarily in the outer medulla in the thick ascending limb and distal convoluted tubule.1 Publication

Gene expression databases

BgeeiENSRNOG00000010872
GenevisibleiP07335 RN

Interactioni

Subunit structurei

Dimer of identical or non-identical chains, which can be either B (brain type) or M (muscle type). With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi2464481 interactor.
CORUMiP07335
IntActiP07335 1 interactor.
MINTiP07335
STRINGi10116.ENSRNOP00000015122

Structurei

3D structure databases

ProteinModelPortaliP07335
SMRiP07335
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 98Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST88
Domaini125 – 367Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST243

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3581 Eukaryota
COG3869 LUCA
GeneTreeiENSGT00550000074561
HOGENOMiHOG000232165
HOVERGENiHBG001339
InParanoidiP07335
KOiK00933
OMAiRGHEFMW
OrthoDBiEOG091G0HZ0
PhylomeDBiP07335
TreeFamiTF314214

Family and domain databases

Gene3Di1.10.135.101 hit
InterProiView protein in InterPro
IPR000749 ATP-guanido_PTrfase
IPR022415 ATP-guanido_PTrfase_AS
IPR022414 ATP-guanido_PTrfase_cat
IPR022413 ATP-guanido_PTrfase_N
IPR036802 ATP-guanido_PTrfase_N_sf
IPR014746 Gln_synth/guanido_kin_cat_dom
PANTHERiPTHR11547 PTHR11547, 1 hit
PfamiView protein in Pfam
PF00217 ATP-gua_Ptrans, 1 hit
PF02807 ATP-gua_PtransN, 1 hit
SUPFAMiSSF48034 SSF48034, 1 hit
SSF55931 SSF55931, 1 hit
PROSITEiView protein in PROSITE
PS00112 PHOSPHAGEN_KINASE, 1 hit
PS51510 PHOSPHAGEN_KINASE_C, 1 hit
PS51509 PHOSPHAGEN_KINASE_N, 1 hit

Sequencei

Sequence statusi: Complete.

P07335-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFSNSHNTQ KLRFPAEDEF PDLSSHNNHM AKVLTPELYA ELRAKCTPSG
60 70 80 90 100
FTLDDAIQTG VDNPGHPYIM TVGAVAGDEE SYDVFKDLFD PIIEDRHGGY
110 120 130 140 150
QPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE
160 170 180 190 200
RRAIEKLAVE ALSSLDGDLS GRYYALKSMT EAEQQQLIDD HFLFDKPVSP
210 220 230 240 250
LLLASGMARD WPDARGIWHN DNKTFLVWIN EEDHLRVISM QKGGNMKEVF
260 270 280 290 300
TRFCTGLTQI ETLFKSKNYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP
310 320 330 340 350
HLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV
360 370 380
QMVVDGVKLL IEMEQRLEQG QPIDDLMPAQ K
Length:381
Mass (Da):42,725
Last modified:January 9, 2007 - v2
Checksum:iA8C8A09F6FF1A4D1
GO

Sequence cautioni

The sequence AAH87656 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18D → A in AAA40931 (PubMed:2284002).Curated1
Sequence conflicti18D → A in AAA40933 (PubMed:2284002).Curated1
Sequence conflicti183 – 184EQ → DE in AAA40930 (PubMed:3005113).Curated2
Sequence conflicti183 – 184EQ → DE in AAA40932 (PubMed:2838389).Curated2
Sequence conflicti206G → A in AAA40932 (PubMed:2838389).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14400 mRNA Translation: AAA40930.1
M18668 Genomic DNA Translation: AAA40932.1
M57664 mRNA Translation: AAA40933.1
M57665 Genomic DNA Translation: AAA40931.1
BC065307 mRNA Translation: AAH65307.2
BC070955 mRNA Translation: AAH70955.2
BC087656 mRNA Translation: AAH87656.1 Different initiation.
BC099814 mRNA Translation: AAH99814.2
BC127477 mRNA Translation: AAI27478.2
PIRiA23980 KIRTCB
RefSeqiNP_036661.3, NM_012529.3
UniGeneiRn.1472

Genome annotation databases

EnsembliENSRNOT00000015122; ENSRNOP00000015122; ENSRNOG00000010872
GeneIDi24264
KEGGirno:24264
UCSCiRGD:2357 rat

Similar proteinsi

Entry informationi

Entry nameiKCRB_RAT
AccessioniPrimary (citable) accession number: P07335
Secondary accession number(s): A0JPK7
, Q499P7, Q5PPJ5, Q6IRE0, Q6P139
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 9, 2007
Last modified: February 28, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome