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Protein

Creatine kinase B-type

Gene

Ckb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei130ATPPROSITE-ProRule annotation1
Binding sitei132ATPPROSITE-ProRule annotation1
Binding sitei191ATPPROSITE-ProRule annotation1
Binding sitei232SubstrateBy similarity1
Binding sitei236ATPPROSITE-ProRule annotation1
Binding sitei285SubstrateBy similarity1
Binding sitei292ATPPROSITE-ProRule annotation1
Binding sitei320ATPPROSITE-ProRule annotation1
Binding sitei335ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi128 – 132ATPPROSITE-ProRule annotation5
Nucleotide bindingi320 – 325ATPPROSITE-ProRule annotation6

GO - Molecular functioni

GO - Biological processi

  • brain development Source: RGD
  • cellular chloride ion homeostasis Source: RGD
  • cerebellum development Source: RGD
  • substantia nigra development Source: Ensembl

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-71288. Creatine metabolism.

Protein family/group databases

MoonProtiP07335.

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase B-type (EC:2.7.3.2)
Alternative name(s):
B-CK
Creatine kinase B chain
Gene namesi
Name:Ckb
Synonyms:Ckbb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi2357. Ckb.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176812.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002119701 – 381Creatine kinase B-typeAdd BLAST381

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineBy similarity1
Modified residuei35PhosphothreonineCombined sources1
Modified residuei125PhosphotyrosineBy similarity1
Modified residuei199PhosphoserineBy similarity1
Modified residuei269Nitrated tyrosineBy similarity1
Modified residuei309PhosphoserineCombined sources1
Modified residuei322PhosphothreonineBy similarity1

Keywords - PTMi

Nitration, Phosphoprotein

Proteomic databases

PaxDbiP07335.
PRIDEiP07335.

2D gel databases

World-2DPAGEi0004:P07335.

PTM databases

iPTMnetiP07335.
PhosphoSitePlusiP07335.

Expressioni

Tissue specificityi

In the kidney localized primarily in the outer medulla in the thick ascending limb and distal convoluted tubule.1 Publication

Gene expression databases

BgeeiENSRNOG00000010872.
GenevisibleiP07335. RN.

Interactioni

Subunit structurei

Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi246448. 1 interactor.
CORUMiP07335.
IntActiP07335. 1 interactor.
MINTiMINT-4586357.
STRINGi10116.ENSRNOP00000015122.

Structurei

3D structure databases

ProteinModelPortaliP07335.
SMRiP07335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 98Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST88
Domaini125 – 367Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST243

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3581. Eukaryota.
COG3869. LUCA.
GeneTreeiENSGT00550000074561.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP07335.
KOiK00933.
OMAiRGHEFMW.
OrthoDBiEOG091G0HZ0.
PhylomeDBiP07335.
TreeFamiTF314214.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR036802. ATP-guanido_PTrfase_N_sf.
IPR014746. Gln_synth/guanido_kin_cat_dom.
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiView protein in Pfam
PF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
SUPFAMiSSF48034. SSF48034. 1 hit.
SSF55931. SSF55931. 1 hit.
PROSITEiView protein in PROSITE
PS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.

Sequencei

Sequence statusi: Complete.

P07335-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFSNSHNTQ KLRFPAEDEF PDLSSHNNHM AKVLTPELYA ELRAKCTPSG
60 70 80 90 100
FTLDDAIQTG VDNPGHPYIM TVGAVAGDEE SYDVFKDLFD PIIEDRHGGY
110 120 130 140 150
QPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE
160 170 180 190 200
RRAIEKLAVE ALSSLDGDLS GRYYALKSMT EAEQQQLIDD HFLFDKPVSP
210 220 230 240 250
LLLASGMARD WPDARGIWHN DNKTFLVWIN EEDHLRVISM QKGGNMKEVF
260 270 280 290 300
TRFCTGLTQI ETLFKSKNYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP
310 320 330 340 350
HLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV
360 370 380
QMVVDGVKLL IEMEQRLEQG QPIDDLMPAQ K
Length:381
Mass (Da):42,725
Last modified:January 9, 2007 - v2
Checksum:iA8C8A09F6FF1A4D1
GO

Sequence cautioni

P07335: The sequence AAH87656 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18D → A in AAA40931 (PubMed:2284002).Curated1
Sequence conflicti18D → A in AAA40933 (PubMed:2284002).Curated1
Sequence conflicti183 – 184EQ → DE in AAA40930 (PubMed:3005113).Curated2
Sequence conflicti183 – 184EQ → DE in AAA40932 (PubMed:2838389).Curated2
Sequence conflicti206G → A in AAA40932 (PubMed:2838389).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14400 mRNA. Translation: AAA40930.1.
M18668 Genomic DNA. Translation: AAA40932.1.
M57664 mRNA. Translation: AAA40933.1.
M57665 Genomic DNA. Translation: AAA40931.1.
BC065307 mRNA. Translation: AAH65307.2.
BC070955 mRNA. Translation: AAH70955.2.
BC087656 mRNA. Translation: AAH87656.1. Different initiation.
BC099814 mRNA. Translation: AAH99814.2.
BC127477 mRNA. Translation: AAI27478.2.
PIRiA23980. KIRTCB.
RefSeqiNP_036661.3. NM_012529.3.
UniGeneiRn.1472.

Genome annotation databases

EnsembliENSRNOT00000015122; ENSRNOP00000015122; ENSRNOG00000010872.
GeneIDi24264.
KEGGirno:24264.
UCSCiRGD:2357. rat.

Similar proteinsi

Entry informationi

Entry nameiKCRB_RAT
AccessioniPrimary (citable) accession number: P07335
Secondary accession number(s): A0JPK7
, Q499P7, Q5PPJ5, Q6IRE0, Q6P139
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 9, 2007
Last modified: October 25, 2017
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families