Creatine kinase B-type - Rattus norvegicus (Rat)

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P07335 (KCRB_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 111. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Creatine kinase B-type
EC=2.7.3.2

Alternative name(s):
B-CK
Creatine kinase B chain

Gene names

Name:	Ckb
Synonyms:	Ckbb

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Catalytic activity	ATP + creatine = ADP + phosphocreatine.
Subunit structure	Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.
Subcellular location	Cytoplasm.
Tissue specificity	In the kidney localized primarily in the outer medulla in the thick ascending limb and distal convoluted tubule. Ref.7
Sequence similarities	Belongs to the ATP:guanido phosphotransferase family. Contains 1 phosphagen kinase C-terminal domain. Contains 1 phosphagen kinase N-terminal domain.
Sequence caution	The sequence AAH87656.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	brain development Inferred from expression pattern PubMed 12093362. Source: RGD cellular chloride ion homeostasis Inferred from mutant phenotype PubMed 16336223. Source: RGD phosphocreatine metabolic process Traceable author statement PubMed 12093362. Source: RGD
Cellular_component	mitochondrion Inferred from electronic annotation. Source: Compara plasma membrane Traceable author statement PubMed 12093362. Source: RGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW creatine kinase activity Traceable author statement PubMed 12093362. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 381

380

Creatine kinase B-type

PRO_0000211970

Regions

Domain

11 – 98

Phosphagen kinase N-terminal

Domain

125 – 367

243

Phosphagen kinase C-terminal

Nucleotide binding

128 – 132

ATP By similarity

Nucleotide binding

320 – 325

ATP By similarity

Sites

Binding site

130

ATP By similarity

Binding site

132

ATP By similarity

Binding site

191

ATP By similarity

Binding site

232

Substrate By similarity

Binding site

236

ATP By similarity

Binding site

285

Substrate By similarity

Binding site

292

ATP By similarity

Binding site

320

ATP By similarity

Binding site

335

ATP By similarity

Amino acid modifications

Modified residue

Phosphoserine By similarity

Modified residue

Phosphothreonine By similarity

Modified residue

Phosphotyrosine By similarity

Modified residue

125

Phosphotyrosine By similarity

Modified residue

164

Phosphoserine By similarity

Modified residue

199

Phosphoserine By similarity

Experimental info

Sequence conflict

D → A in AAA40931. Ref.3

Sequence conflict

D → A in AAA40933. Ref.3

Sequence conflict

183 – 184

EQ → DE in AAA40930. Ref.1

Sequence conflict

183 – 184

EQ → DE in AAA40932. Ref.2

Sequence conflict

206

G → A in AAA40932. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P07335 [UniParc].

Last modified January 9, 2007. Version 2.
Checksum: A8C8A09F6FF1A4D1
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FASTA

381

42,725

        10         20         30         40         50         60 
MPFSNSHNTQ KLRFPAEDEF PDLSSHNNHM AKVLTPELYA ELRAKCTPSG FTLDDAIQTG 

        70         80         90        100        110        120 
VDNPGHPYIM TVGAVAGDEE SYDVFKDLFD PIIEDRHGGY QPSDEHKTDL NPDNLQGGDD 

       130        140        150        160        170        180 
LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE RRAIEKLAVE ALSSLDGDLS GRYYALKSMT 

       190        200        210        220        230        240 
EAEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWIN EEDHLRVISM 

       250        260        270        280        290        300 
QKGGNMKEVF TRFCTGLTQI ETLFKSKNYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP 

       310        320        330        340        350        360 
HLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV QMVVDGVKLL 

       370        380 
IEMEQRLEQG QPIDDLMPAQ K

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of a rat brain creatine kinase-beta-galactosidase fusion protein in Escherichia coli and derivation of the complete amino acid sequence of rat brain creatine kinase." Benfield P.A., Henderson L., Pearson M.L. Gene 39:263-267(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation of four rat creatine kinase genes and identification of multiple potential promoter sequences within the rat brain creatine kinase promoter region." Benfield P.A., Graf D., Korolkoff P.N., Hobson G., Pearson M.L. Gene 63:227-243(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Estrogen regulation of creatine kinase-B in the rat uterus." Pentecost B.T., Mattheiss L., Dickerman H.W., Kumar S.A. Mol. Endocrinol. 4:1000-1010(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Uterus.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Embryonic brain, Heart and Prostate.
[5]	Lubec G., Afjehi-Sadat L., Diao W., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 33-43; 87-96; 108-130; 138-148; 157-172; 224-236; 253-265 AND 320-341, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.
[6]	"The brain isoform of a key ATP-regulating enzyme, creatine kinase, is a phosphoprotein." Mahadevan L.C., Whatley S.A., Leung T.K.C., Lim L. Biochem. J. 222:139-144(1984) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION.
[7]	"Compartmentation of multiple forms of creatine kinase in the distal nephron of the rat kidney." Friedman D.L., Perryman M.B. J. Biol. Chem. 266:22404-22410(1991) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M14400 mRNA. Translation: AAA40930.1. M18668 Genomic DNA. Translation: AAA40932.1. M57664 mRNA. Translation: AAA40933.1. M57665 Genomic DNA. Translation: AAA40931.1. BC065307 mRNA. Translation: AAH65307.2. BC070955 mRNA. Translation: AAH70955.2. BC087656 mRNA. Translation: AAH87656.1. Different initiation. BC099814 mRNA. Translation: AAH99814.2. BC127477 mRNA. Translation: AAI27478.2.
IPI	IPI00470288.
PIR	KIRTCB. A23980.
RefSeq	NP_036661.3. NM_012529.3.
UniGene	Rn.1472.
3D structure databases
ProteinModelPortal	P07335.
SMR	P07335. Positions 2-381.
ModBase	Search...
PTM databases
PhosphoSite	P07335.
2D gel databases
World-2DPAGE	0004:P07335.
Proteomic databases
PaxDb	P07335.
PRIDE	P07335.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000015122; ENSRNOP00000015122; ENSRNOG00000010872.
GeneID	24264.
KEGG	rno:24264.
UCSC	RGD:2357. rat.
Organism-specific databases
CTD	1152.
RGD	2357. Ckb.
Phylogenomic databases
eggNOG	COG3869.
GeneTree	ENSGT00550000074561.
HOGENOM	HOG000232165.
HOVERGEN	HBG001339.
InParanoid	P07335.
KO	K00933.
OrthoDB	EOG4R7V9X.
Gene expression databases
Genevestigator	P07335.
GermOnline	ENSRNOG00000010872. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.135.10. 1 hit. 3.30.590.10. 1 hit.
InterPro	IPR000749. ATP-guanido_PTrfase. IPR022415. ATP-guanido_PTrfase_AS. IPR022414. ATP-guanido_PTrfase_cat. IPR022413. ATP-guanido_PTrfase_N. IPR014746. Gln_synth/guanido_kin_cat_dom. [Graphical view]
PANTHER	PTHR11547. PTHR11547. 1 hit.
Pfam	PF00217. ATP-gua_Ptrans. 1 hit. PF02807. ATP-gua_PtransN. 1 hit. [Graphical view]
SUPFAM	SSF48034. ATP-gua_Ptrans. 1 hit.
PROSITE	PS00112. PHOSPHAGEN_KINASE. 1 hit. PS51510. PHOSPHAGEN_KINASE_C. 1 hit. PS51509. PHOSPHAGEN_KINASE_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	602815.

Entry information

Entry name

KCRB_RAT

Accession

Primary (citable) accession number: P07335
Secondary accession number(s): A0JPK7

Q6P139

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 9, 2007
Last modified:	April 3, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents