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P07330 (CHEB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chemotaxis response regulator protein-glutamate methylesterase

EC=3.1.1.61
Gene names
Name:cheB
Ordered Locus Names:b1883, JW1872
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR. HAMAP-Rule MF_00099

Catalytic activity

Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP-Rule MF_00099

Subcellular location

Cytoplasm HAMAP-Rule MF_00099.

Domain

The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP-Rule MF_00099

Post-translational modification

Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain. Ref.5

Sequence similarities

Contains 1 cheB-type methylesterase domain.

Contains 1 response regulatory domain.

Ontologies

Keywords
   Biological processChemotaxis
   Cellular componentCytoplasm
   Molecular functionHydrolase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processchemotaxis

Inferred from mutant phenotype PubMed 4895809. Source: EcoliWiki

sensory perception of chemical stimulus

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionphosphorelay response regulator activity

Inferred from electronic annotation. Source: InterPro

protein-glutamate methylesterase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349Chemotaxis response regulator protein-glutamate methylesterase HAMAP-Rule MF_00099
PRO_0000157992

Regions

Domain5 – 122118Response regulatory
Domain152 – 344193CheB-type methylesterase

Sites

Active site1641 By similarity
Active site1901 By similarity
Active site2861 By similarity

Amino acid modifications

Modified residue5614-aspartylphosphate By similarity

Experimental info

Sequence conflict961A → P in AAA23569. Ref.1
Sequence conflict1251E → Q in AAA23569. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P07330 [UniParc].

Last modified November 1, 1997. Version 3.
Checksum: 6F3D1AFB8D23D3F5

FASTA34937,468
        10         20         30         40         50         60 
MSKIRVLSVD DSALMRQIMT EIINSHSDME MVATAPDPLV ARDLIKKFNP DVLTLDVEMP 

        70         80         90        100        110        120 
RMDGLDFLEK LMRLRPMPVV MVSSLTGKGS EVTLRALELG AIDFVTKPQL GIREGMLAYN 

       130        140        150        160        170        180 
EMIAEKVRTA AKASLAAHKP LSAPTTLKAG PLLSSEKLIA IGASTGGTEA IRHVLQPLPL 

       190        200        210        220        230        240 
SSPALLITQH MPPGFTRSFA DRLNKLCQIG VKEAEDGERV LPGHAYIAPG DRHMELSRSG 

       250        260        270        280        290        300 
ANYQIKIHDG PAVNRHRPSV DVLFHSVAKQ AGRNAVGVIL TGMGNDGAAG MLAMRQAGAW 

       310        320        330        340 
TLAQNEASCV VFGMPREAIN MGGVCEVVDL SQVSQQMLAK ISAGQAIRI 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence corresponding to five chemotaxis genes in Escherichia coli."
Mutoh N., Simon M.I.
J. Bacteriol. 165:161-166(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis."
Hess J.F., Oosawa K., Kaplan N., Simon M.I.
Cell 53:79-87(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M13463 Genomic DNA. Translation: AAA23569.1.
U00096 Genomic DNA. Translation: AAC74953.1.
AP009048 Genomic DNA. Translation: BAA15699.1.
PIRXYECEB. D25195.
RefSeqNP_416397.1. NC_000913.3.
YP_490145.1. NC_007779.1.

3D structure databases

ProteinModelPortalP07330.
SMRP07330. Positions 1-347.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-9272N.
IntActP07330. 10 interactions.
STRING511145.b1883.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74953; AAC74953; b1883.
BAA15699; BAA15699; BAA15699.
GeneID12931722.
946394.
KEGGecj:Y75_p1859.
eco:b1883.
PATRIC32119093. VBIEscCol129921_1964.

Organism-specific databases

EchoBASEEB0145.
EcoGeneEG10147. cheB.

Phylogenomic databases

eggNOGCOG2201.
HOGENOMHOG000151423.
KOK03412.
OMAANYQVKL.
OrthoDBEOG6PKFC7.
PhylomeDBP07330.
ProtClustDBPRK00742.

Enzyme and pathway databases

BioCycEcoCyc:CHEB-MONOMER.
ECOL316407:JW1872-MONOMER.
MetaCyc:CHEB-MONOMER.
BRENDA3.1.1.61. 2026.

Gene expression databases

GenevestigatorP07330.

Family and domain databases

Gene3D3.40.50.180. 1 hit.
HAMAPMF_00099. CheB_methylest.
InterProIPR011006. CheY-like_superfamily.
IPR008248. Sig_transdc_resp-reg_CheB.
IPR000673. Sig_transdc_resp-reg_Me-estase.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamPF01339. CheB_methylest. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFPIRSF000876. RR_chemtxs_CheB. 1 hit.
SMARTSM00448. REC. 1 hit.
[Graphical view]
SUPFAMSSF52172. SSF52172. 1 hit.
SSF52738. SSF52738. 1 hit.
PROSITEPS50122. CHEB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP07330.

Entry information

Entry nameCHEB_ECOLI
AccessionPrimary (citable) accession number: P07330
Secondary accession number(s): P78070
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene