P07330 (CHEB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase
EC=3.1.1.61

Gene names

Name:	cheB
Ordered Locus Names:	b1883, JW1872

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR. HAMAP-Rule MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP-Rule MF_00099
Subcellular location	Cytoplasm HAMAP-Rule MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP-Rule MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain. Ref.5
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chemotaxis Inferred from mutant phenotype PubMed 4895809. Source: EcoliWiki intracellular signal transduction Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Chemotaxis response regulator protein-glutamate methylesterase HAMAP-Rule MF_00099		PRO_0000157992
Regions
	Domain	5 – 122	118	Response regulatory
	Domain	152 – 344	193	CheB-type methylesterase
Sites
	Active site	164	1	By similarity
	Active site	190	1	By similarity
	Active site	286	1	By similarity
Amino acid modifications
	Modified residue	56	1	4-aspartylphosphate By similarity
Experimental info
	Sequence conflict	96	1	A → P in AAA23569. Ref.1
	Sequence conflict	125	1	E → Q in AAA23569. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P07330 [UniParc]. Last modified November 1, 1997. Version 3. Checksum: 6F3D1AFB8D23D3F5	FASTA	349	37,468
	10 20 30 40 50 60 MSKIRVLSVD DSALMRQIMT EIINSHSDME MVATAPDPLV ARDLIKKFNP DVLTLDVEMP 70 80 90 100 110 120 RMDGLDFLEK LMRLRPMPVV MVSSLTGKGS EVTLRALELG AIDFVTKPQL GIREGMLAYN 130 140 150 160 170 180 EMIAEKVRTA AKASLAAHKP LSAPTTLKAG PLLSSEKLIA IGASTGGTEA IRHVLQPLPL 190 200 210 220 230 240 SSPALLITQH MPPGFTRSFA DRLNKLCQIG VKEAEDGERV LPGHAYIAPG DRHMELSRSG 250 260 270 280 290 300 ANYQIKIHDG PAVNRHRPSV DVLFHSVAKQ AGRNAVGVIL TGMGNDGAAG MLAMRQAGAW 310 320 330 340 TLAQNEASCV VFGMPREAIN MGGVCEVVDL SQVSQQMLAK ISAGQAIRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence corresponding to five chemotaxis genes in Escherichia coli." Mutoh N., Simon M.I. J. Bacteriol. 165:161-166(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis." Hess J.F., Oosawa K., Kaplan N., Simon M.I. Cell 53:79-87(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M13463 Genomic DNA. Translation: AAA23569.1. U00096 Genomic DNA. Translation: AAC74953.1. AP009048 Genomic DNA. Translation: BAA15699.1.
PIR	XYECEB. D25195.
RefSeq	NP_416397.1. NC_000913.2. YP_490145.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P07330.
SMR	P07330. Positions 1-347.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9272N.
IntAct	P07330. 10 interactions.
STRING	511145.b1883.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74953; AAC74953; b1883. BAA15699; BAA15699; BAA15699.
GeneID	12931722. 946394.
KEGG	ecj:Y75_p1859. eco:b1883.
PATRIC	32119093. VBIEscCol129921_1964.
Organism-specific databases
EchoBASE	EB0145.
EcoGene	EG10147. cheB.
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HOG000151423.
KO	K03412.
OMA	EAPVNRH.
ProtClustDB	PRK00742.
Enzyme and pathway databases
BioCyc	EcoCyc:CHEB-MONOMER. ECOL316407:JW1872-MONOMER. MetaCyc:CHEB-MONOMER.
BRENDA	3.1.1.61. 2026.
Gene expression databases
Genevestigator	P07330.
Family and domain databases
Gene3D	3.40.50.180. 1 hit.
HAMAP	MF_00099. CheB_methylest.
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB_ECOLI

Accession

Primary (citable) accession number: P07330
Secondary accession number(s): P78070

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 124 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program