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P07330

- CHEB_ECOLI

UniProt

P07330 - CHEB_ECOLI

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Protein

Chemotaxis response regulator protein-glutamate methylesterase

Gene

cheB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR.

Catalytic activityi

Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei164 – 1641By similarity
Active sitei190 – 1901By similarity
Active sitei286 – 2861By similarity

GO - Molecular functioni

  1. phosphorelay response regulator activity Source: InterPro
  2. protein-glutamate methylesterase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. chemotaxis Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Chemotaxis

Enzyme and pathway databases

BioCyciEcoCyc:CHEB-MONOMER.
ECOL316407:JW1872-MONOMER.
MetaCyc:CHEB-MONOMER.
BRENDAi3.1.1.61. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase (EC:3.1.1.61)
Gene namesi
Name:cheB
Ordered Locus Names:b1883, JW1872
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10147. cheB.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: EcoCyc
  2. plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Chemotaxis response regulator protein-glutamate methylesterasePRO_0000157992Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 5614-aspartylphosphateBy similarity

Post-translational modificationi

Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain.1 Publication

Keywords - PTMi

Phosphoprotein

Expressioni

Gene expression databases

GenevestigatoriP07330.

Interactioni

Protein-protein interaction databases

DIPiDIP-9272N.
IntActiP07330. 10 interactions.
STRINGi511145.b1883.

Structurei

3D structure databases

ProteinModelPortaliP07330.
SMRiP07330. Positions 1-347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 122118Response regulatoryAdd
BLAST
Domaini152 – 344193CheB-type methylesteraseAdd
BLAST

Domaini

The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain.

Sequence similaritiesi

Contains 1 response regulatory domain.Curated

Phylogenomic databases

eggNOGiCOG2201.
HOGENOMiHOG000151423.
InParanoidiP07330.
KOiK03412.
OMAiEVVDLHQ.
OrthoDBiEOG6PKFC7.
PhylomeDBiP07330.

Family and domain databases

Gene3Di3.40.50.180. 1 hit.
HAMAPiMF_00099. CheB_methylest.
InterProiIPR011006. CheY-like_superfamily.
IPR008248. Sig_transdc_resp-reg_CheB.
IPR000673. Sig_transdc_resp-reg_Me-estase.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF01339. CheB_methylest. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFiPIRSF000876. RR_chemtxs_CheB. 1 hit.
SMARTiSM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
SSF52738. SSF52738. 1 hit.
PROSITEiPS50122. CHEB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07330-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKIRVLSVD DSALMRQIMT EIINSHSDME MVATAPDPLV ARDLIKKFNP
60 70 80 90 100
DVLTLDVEMP RMDGLDFLEK LMRLRPMPVV MVSSLTGKGS EVTLRALELG
110 120 130 140 150
AIDFVTKPQL GIREGMLAYN EMIAEKVRTA AKASLAAHKP LSAPTTLKAG
160 170 180 190 200
PLLSSEKLIA IGASTGGTEA IRHVLQPLPL SSPALLITQH MPPGFTRSFA
210 220 230 240 250
DRLNKLCQIG VKEAEDGERV LPGHAYIAPG DRHMELSRSG ANYQIKIHDG
260 270 280 290 300
PAVNRHRPSV DVLFHSVAKQ AGRNAVGVIL TGMGNDGAAG MLAMRQAGAW
310 320 330 340
TLAQNEASCV VFGMPREAIN MGGVCEVVDL SQVSQQMLAK ISAGQAIRI
Length:349
Mass (Da):37,468
Last modified:November 1, 1997 - v3
Checksum:i6F3D1AFB8D23D3F5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti96 – 961A → P in AAA23569. (PubMed:3510184)Curated
Sequence conflicti125 – 1251E → Q in AAA23569. (PubMed:3510184)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13463 Genomic DNA. Translation: AAA23569.1.
U00096 Genomic DNA. Translation: AAC74953.1.
AP009048 Genomic DNA. Translation: BAA15699.1.
PIRiD25195. XYECEB.
RefSeqiNP_416397.1. NC_000913.3.
YP_490145.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74953; AAC74953; b1883.
BAA15699; BAA15699; BAA15699.
GeneIDi12931722.
946394.
KEGGiecj:Y75_p1859.
eco:b1883.
PATRICi32119093. VBIEscCol129921_1964.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13463 Genomic DNA. Translation: AAA23569.1 .
U00096 Genomic DNA. Translation: AAC74953.1 .
AP009048 Genomic DNA. Translation: BAA15699.1 .
PIRi D25195. XYECEB.
RefSeqi NP_416397.1. NC_000913.3.
YP_490145.1. NC_007779.1.

3D structure databases

ProteinModelPortali P07330.
SMRi P07330. Positions 1-347.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9272N.
IntActi P07330. 10 interactions.
STRINGi 511145.b1883.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74953 ; AAC74953 ; b1883 .
BAA15699 ; BAA15699 ; BAA15699 .
GeneIDi 12931722.
946394.
KEGGi ecj:Y75_p1859.
eco:b1883.
PATRICi 32119093. VBIEscCol129921_1964.

Organism-specific databases

EchoBASEi EB0145.
EcoGenei EG10147. cheB.

Phylogenomic databases

eggNOGi COG2201.
HOGENOMi HOG000151423.
InParanoidi P07330.
KOi K03412.
OMAi EVVDLHQ.
OrthoDBi EOG6PKFC7.
PhylomeDBi P07330.

Enzyme and pathway databases

BioCyci EcoCyc:CHEB-MONOMER.
ECOL316407:JW1872-MONOMER.
MetaCyc:CHEB-MONOMER.
BRENDAi 3.1.1.61. 2026.

Miscellaneous databases

PROi P07330.

Gene expression databases

Genevestigatori P07330.

Family and domain databases

Gene3Di 3.40.50.180. 1 hit.
HAMAPi MF_00099. CheB_methylest.
InterProi IPR011006. CheY-like_superfamily.
IPR008248. Sig_transdc_resp-reg_CheB.
IPR000673. Sig_transdc_resp-reg_Me-estase.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view ]
Pfami PF01339. CheB_methylest. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view ]
PIRSFi PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMARTi SM00448. REC. 1 hit.
[Graphical view ]
SUPFAMi SSF52172. SSF52172. 1 hit.
SSF52738. SSF52738. 1 hit.
PROSITEi PS50122. CHEB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence corresponding to five chemotaxis genes in Escherichia coli."
    Mutoh N., Simon M.I.
    J. Bacteriol. 165:161-166(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis."
    Hess J.F., Oosawa K., Kaplan N., Simon M.I.
    Cell 53:79-87(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.

Entry informationi

Entry nameiCHEB_ECOLI
AccessioniPrimary (citable) accession number: P07330
Secondary accession number(s): P78070
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3