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P07330

- CHEB_ECOLI

UniProt

P07330 - CHEB_ECOLI

Protein

Chemotaxis response regulator protein-glutamate methylesterase

Gene

cheB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 3 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR.

    Catalytic activityi

    Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei164 – 1641By similarity
    Active sitei190 – 1901By similarity
    Active sitei286 – 2861By similarity

    GO - Molecular functioni

    1. phosphorelay response regulator activity Source: InterPro
    2. protein-glutamate methylesterase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. chemotaxis Source: EcoliWiki

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Chemotaxis

    Enzyme and pathway databases

    BioCyciEcoCyc:CHEB-MONOMER.
    ECOL316407:JW1872-MONOMER.
    MetaCyc:CHEB-MONOMER.
    BRENDAi3.1.1.61. 2026.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase (EC:3.1.1.61)
    Gene namesi
    Name:cheB
    Ordered Locus Names:b1883, JW1872
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10147. cheB.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: EcoCyc
    2. plasma membrane Source: EcoCyc

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 349349Chemotaxis response regulator protein-glutamate methylesterasePRO_0000157992Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei56 – 5614-aspartylphosphateBy similarity

    Post-translational modificationi

    Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriP07330.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-9272N.
    IntActiP07330. 10 interactions.
    STRINGi511145.b1883.

    Structurei

    3D structure databases

    ProteinModelPortaliP07330.
    SMRiP07330. Positions 1-347.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 122118Response regulatoryAdd
    BLAST
    Domaini152 – 344193CheB-type methylesteraseAdd
    BLAST

    Domaini

    The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain.

    Sequence similaritiesi

    Contains 1 response regulatory domain.Curated

    Phylogenomic databases

    eggNOGiCOG2201.
    HOGENOMiHOG000151423.
    KOiK03412.
    OMAiEVVDLHQ.
    OrthoDBiEOG6PKFC7.
    PhylomeDBiP07330.

    Family and domain databases

    Gene3Di3.40.50.180. 1 hit.
    HAMAPiMF_00099. CheB_methylest.
    InterProiIPR011006. CheY-like_superfamily.
    IPR008248. Sig_transdc_resp-reg_CheB.
    IPR000673. Sig_transdc_resp-reg_Me-estase.
    IPR001789. Sig_transdc_resp-reg_receiver.
    [Graphical view]
    PfamiPF01339. CheB_methylest. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000876. RR_chemtxs_CheB. 1 hit.
    SMARTiSM00448. REC. 1 hit.
    [Graphical view]
    SUPFAMiSSF52172. SSF52172. 1 hit.
    SSF52738. SSF52738. 1 hit.
    PROSITEiPS50122. CHEB. 1 hit.
    PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07330-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKIRVLSVD DSALMRQIMT EIINSHSDME MVATAPDPLV ARDLIKKFNP    50
    DVLTLDVEMP RMDGLDFLEK LMRLRPMPVV MVSSLTGKGS EVTLRALELG 100
    AIDFVTKPQL GIREGMLAYN EMIAEKVRTA AKASLAAHKP LSAPTTLKAG 150
    PLLSSEKLIA IGASTGGTEA IRHVLQPLPL SSPALLITQH MPPGFTRSFA 200
    DRLNKLCQIG VKEAEDGERV LPGHAYIAPG DRHMELSRSG ANYQIKIHDG 250
    PAVNRHRPSV DVLFHSVAKQ AGRNAVGVIL TGMGNDGAAG MLAMRQAGAW 300
    TLAQNEASCV VFGMPREAIN MGGVCEVVDL SQVSQQMLAK ISAGQAIRI 349
    Length:349
    Mass (Da):37,468
    Last modified:November 1, 1997 - v3
    Checksum:i6F3D1AFB8D23D3F5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti96 – 961A → P in AAA23569. (PubMed:3510184)Curated
    Sequence conflicti125 – 1251E → Q in AAA23569. (PubMed:3510184)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13463 Genomic DNA. Translation: AAA23569.1.
    U00096 Genomic DNA. Translation: AAC74953.1.
    AP009048 Genomic DNA. Translation: BAA15699.1.
    PIRiD25195. XYECEB.
    RefSeqiNP_416397.1. NC_000913.3.
    YP_490145.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74953; AAC74953; b1883.
    BAA15699; BAA15699; BAA15699.
    GeneIDi12931722.
    946394.
    KEGGiecj:Y75_p1859.
    eco:b1883.
    PATRICi32119093. VBIEscCol129921_1964.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13463 Genomic DNA. Translation: AAA23569.1 .
    U00096 Genomic DNA. Translation: AAC74953.1 .
    AP009048 Genomic DNA. Translation: BAA15699.1 .
    PIRi D25195. XYECEB.
    RefSeqi NP_416397.1. NC_000913.3.
    YP_490145.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P07330.
    SMRi P07330. Positions 1-347.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9272N.
    IntActi P07330. 10 interactions.
    STRINGi 511145.b1883.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74953 ; AAC74953 ; b1883 .
    BAA15699 ; BAA15699 ; BAA15699 .
    GeneIDi 12931722.
    946394.
    KEGGi ecj:Y75_p1859.
    eco:b1883.
    PATRICi 32119093. VBIEscCol129921_1964.

    Organism-specific databases

    EchoBASEi EB0145.
    EcoGenei EG10147. cheB.

    Phylogenomic databases

    eggNOGi COG2201.
    HOGENOMi HOG000151423.
    KOi K03412.
    OMAi EVVDLHQ.
    OrthoDBi EOG6PKFC7.
    PhylomeDBi P07330.

    Enzyme and pathway databases

    BioCyci EcoCyc:CHEB-MONOMER.
    ECOL316407:JW1872-MONOMER.
    MetaCyc:CHEB-MONOMER.
    BRENDAi 3.1.1.61. 2026.

    Miscellaneous databases

    PROi P07330.

    Gene expression databases

    Genevestigatori P07330.

    Family and domain databases

    Gene3Di 3.40.50.180. 1 hit.
    HAMAPi MF_00099. CheB_methylest.
    InterProi IPR011006. CheY-like_superfamily.
    IPR008248. Sig_transdc_resp-reg_CheB.
    IPR000673. Sig_transdc_resp-reg_Me-estase.
    IPR001789. Sig_transdc_resp-reg_receiver.
    [Graphical view ]
    Pfami PF01339. CheB_methylest. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000876. RR_chemtxs_CheB. 1 hit.
    SMARTi SM00448. REC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52172. SSF52172. 1 hit.
    SSF52738. SSF52738. 1 hit.
    PROSITEi PS50122. CHEB. 1 hit.
    PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence corresponding to five chemotaxis genes in Escherichia coli."
      Mutoh N., Simon M.I.
      J. Bacteriol. 165:161-166(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis."
      Hess J.F., Oosawa K., Kaplan N., Simon M.I.
      Cell 53:79-87(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION.

    Entry informationi

    Entry nameiCHEB_ECOLI
    AccessioniPrimary (citable) accession number: P07330
    Secondary accession number(s): P78070
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 133 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3