Reviewed, UniProtKB/Swiss-Prot P07322 (ENOB_CHICK)

Names and origin

Protein names

Recommended name:
    Beta-enolase
    EC=4.2.1.11
Alternative name(s):
    2-phospho-D-glycerate hydro-lyase
    Phosphopyruvate hydratase

Gene names

Name:

ENO3

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	2-phospho-D-glycerate = phosphoenolpyruvate + H2O.
Cofactor	Magnesium. Required for catalysis and for stabilizing the dimer.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Subunit structure	Homodimer. Interacts with PNKD By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the enolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Lyase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	phosphopyruvate hydratase complex Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphopyruvate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 434	433	Beta-enolase		PRO_0000134111
Regions
	Region	370 – 373	4	Substrate binding By similarity
Sites
	Active site	210	1	Proton donor By similarity
	Active site	343	1	Proton acceptor By similarity
	Metal binding	245	1	Magnesium By similarity
	Metal binding	293	1	Magnesium By similarity
	Metal binding	318	1	Magnesium By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	293	1	Substrate By similarity
	Binding site	318	1	Substrate By similarity
	Binding site	394	1	Substrate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.3
Experimental info
	Sequence conflict	17	1	E → D AA sequence Ref.2
	Sequence conflict	49	1	P → L AA sequence Ref.2
	Sequence conflict	94	1	M → V AA sequence Ref.2
	Sequence conflict	119 – 120	2	CK → SH AA sequence Ref.2
	Sequence conflict	209	1	G → D AA sequence Ref.2
	Sequence conflict	258	1	H → D AA sequence Ref.2
	Sequence conflict	266 – 267	2	HT → DP AA sequence Ref.2
	Sequence conflict	270	1	Y → L AA sequence Ref.2
	Sequence conflict	309	1	F → S AA sequence Ref.2
	Sequence conflict	323	1	T → A AA sequence Ref.2
	Sequence conflict	331	1	G → A AA sequence Ref.2
	Sequence conflict	343	1	K → G AA sequence Ref.2
	Sequence conflict	394 – 395	2	KT → EQ AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P07322-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 892D1BE4B6342F44	FASTA	434	47,196
	10 20 30 40 50 60 MSIQKIHARE ILDSRGEPTV EVDLHTAKGH FRAAVPSGAS TGIHEALEPR DGDKKRFLGK 70 80 90 100 110 120 GVLKAVEHIN KTIGPALIEK KISVVEQEKI DKVMIEMDGT ENKSKFGANA ILGVSLAVCK 130 140 150 160 170 180 AGAAEKGVPL YRHIADLAGN TELILPVPAF NVINGGSHAG NKLAMQEFMV LPVGAASFHD 190 200 210 220 230 240 AMRVGAEVYH SLKGVIKAKY GKDATNVGGE GGFAPNILDN HEALELLKAA IAQAGYTDKV 250 260 270 280 290 300 VIGMDVAASE FCRDGRYHLD FKSPPHTKRY ITGEQLGEIY RGFIKDYPVV SIEDPFDQDD 310 320 330 340 350 360 WEAWKRFVFH VDIQVVGDDL TVTNPKRIAH GAEQHACNCL LLKVNQIGSV TESIQACKLA 370 380 390 400 410 420 QSHGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEALGDK 430 AKFAGRKFRN PKAK

References

[1]	"Chicken alpha-enolase but not beta-enolase has a Src-dependent tyrosine-phosphorylation site: cDNA cloning and nucleotide sequence analysis." Tanaka M., Maeda K., Nakashima K. J. Biochem. 117:554-559(1995) [PubMed: 7629021] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: White leghorn. Tissue: Muscle.
[2]	"The complete amino acid sequence of chicken skeletal-muscle enolase." Russell G.A., Dunbar B., Fothergill-Gilmore L.A. Biochem. J. 236:115-126(1986) [PubMed: 3539098] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-434.
[3]	"Structural elucidation of N-terminal post-translational modifications by mass spectrometry: application to chicken enolase and the alpha- and beta-subunits of bovine mitochondrial F1-ATPase." Gibson B.W., Daley D.J., Williams D.H. Anal. Biochem. 169:217-226(1988) [PubMed: 2898218] [Abstract] Cited for: ACETYLATION AT SER-2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D37901 mRNA. Translation: BAA07133.1.
IPI	IPI00586105.
PIR	A23850. JC4187.
RefSeq	NP_990450.1.
UniGene	Gga.4297
3D structure databases
HSSP	HSSP built from PDB template 1OEP based on UniProtKB Q9NDH8.
SMR	P07322. Positions 2-431.
ModBase	Search...
Proteomic databases
PRIDE	P07322.
Genome annotation databases
GeneID	396016.
KEGG	gga:396016.
Phylogenomic databases
HOVERGEN	P07322.
Enzyme and pathway databases
BRENDA	4.2.1.11. 4.
Family and domain databases
InterPro	IPR000941. Enolase. [Graphical view]
PANTHER	PTHR11902. Enolase. 1 hit.
Pfam	PF00113. Enolase_C. 1 hit. PF03952. Enolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF001400. Enolase. 1 hit.
PRINTS	PR00148. ENOLASE.
ProDom	PD000902. Enolase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01060. eno. 1 hit.
PROSITE	PS00164. ENOLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENOB_CHICK

Accession

Primary (citable) accession number: P07322

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)