ID   EPO_MOUSE               Reviewed;         192 AA.
AC   P07321;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   24-JAN-2024, entry version 173.
DE   RecName: Full=Erythropoietin;
DE   Flags: Precursor;
GN   Name=Epo;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3773894; DOI=10.1128/mcb.6.3.849-858.1986;
RA   Shoemaker C.B., Mitsock L.D.;
RT   "Murine erythropoietin gene: cloning, expression, and human gene
RT   homology.";
RL   Mol. Cell. Biol. 6:849-858(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3022133; DOI=10.1128/mcb.6.3.842-848.1986;
RA   McDonald J.D., Lin F.-K., Goldwasser E.;
RT   "Cloning, sequencing, and evolutionary analysis of the mouse erythropoietin
RT   gene.";
RL   Mol. Cell. Biol. 6:842-848(1986).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/Sv;
RX   PubMed=11239002; DOI=10.1093/nar/29.6.1352;
RA   Wilson M.D., Riemer C., Martindale D.W., Schnupf P., Boright A.P.,
RA   Cheung T.L., Hardy D.M., Schwartz S., Scherer S.W., Tsui L.-C., Miller W.,
RA   Koop B.F.;
RT   "Comparative analysis of the gene-dense ACHE/TFR2 region on human
RT   chromosome 7q22 with the orthologous region on mouse chromosome 5.";
RL   Nucleic Acids Res. 29:1352-1365(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-52.
RC   STRAIN=ICFW;
RX   PubMed=9365246; DOI=10.1038/sj.onc.1201364;
RA   Chretien S., Duprez V., Maouche L., Gisselbrecht S., Mayeux P., Lacombe C.;
RT   "Abnormal erythropoietin (Epo) gene expression in the murine
RT   erythroleukemia IW32 cells results from a rearrangement between the G-
RT   protein beta2 subunit gene and the Epo gene.";
RL   Oncogene 15:1995-1999(1997).
CC   -!- FUNCTION: Hormone involved in the regulation of erythrocyte
CC       proliferation and differentiation and the maintenance of a
CC       physiological level of circulating erythrocyte mass. Binds to EPOR
CC       leading to EPOR dimerization and JAK2 activation thereby activating
CC       specific downstream effectors, including STAT1 and STAT3.
CC       {ECO:0000250|UniProtKB:P01588}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Produced by kidney or liver of adult mammals and by
CC       liver of fetal or neonatal mammals.
CC   -!- SIMILARITY: Belongs to the EPO/TPO family. {ECO:0000305}.
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DR   EMBL; M12482; AAA37568.1; -; Genomic_DNA.
DR   EMBL; M12930; AAA37570.1; -; Genomic_DNA.
DR   EMBL; AF312033; AAK28825.1; -; Genomic_DNA.
DR   EMBL; Y11971; CAA72707.1; -; mRNA.
DR   CCDS; CCDS39332.1; -.
DR   PIR; A24902; A24902.
DR   RefSeq; NP_031968.1; NM_007942.2.
DR   AlphaFoldDB; P07321; -.
DR   SMR; P07321; -.
DR   STRING; 10090.ENSMUSP00000106667; -.
DR   GlyCosmos; P07321; 3 sites, No reported glycans.
DR   GlyGen; P07321; 3 sites.
DR   PhosphoSitePlus; P07321; -.
DR   PaxDb; 10090-ENSMUSP00000031723; -.
DR   Antibodypedia; 4151; 1405 antibodies from 40 providers.
DR   DNASU; 13856; -.
DR   Ensembl; ENSMUST00000031723.14; ENSMUSP00000031723.7; ENSMUSG00000029711.14.
DR   Ensembl; ENSMUST00000111038.8; ENSMUSP00000106667.2; ENSMUSG00000029711.14.
DR   GeneID; 13856; -.
DR   KEGG; mmu:13856; -.
DR   UCSC; uc009acm.1; mouse.
DR   AGR; MGI:95407; -.
DR   CTD; 2056; -.
DR   MGI; MGI:95407; Epo.
DR   VEuPathDB; HostDB:ENSMUSG00000029711; -.
DR   eggNOG; ENOG502RXRC; Eukaryota.
DR   GeneTree; ENSGT00390000017226; -.
DR   InParanoid; P07321; -.
DR   OMA; AMEFPRL; -.
DR   OrthoDB; 4338688at2759; -.
DR   PhylomeDB; P07321; -.
DR   TreeFam; TF333413; -.
DR   Reactome; R-MMU-9027276; Erythropoietin activates Phosphoinositide-3-kinase (PI3K).
DR   Reactome; R-MMU-9027284; Erythropoietin activates RAS.
DR   SABIO-RK; P07321; -.
DR   BioGRID-ORCS; 13856; 1 hit in 80 CRISPR screens.
DR   PRO; PR:P07321; -.
DR   Proteomes; UP000000589; Chromosome 5.
DR   RNAct; P07321; Protein.
DR   Bgee; ENSMUSG00000029711; Expressed in mesodermal cell in embryo and 33 other cell types or tissues.
DR   ExpressionAtlas; P07321; baseline and differential.
DR   GO; GO:0044297; C:cell body; ISO:MGI.
DR   GO; GO:0009986; C:cell surface; ISO:MGI.
DR   GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR   GO; GO:0005615; C:extracellular space; IDA:MGI.
DR   GO; GO:0005125; F:cytokine activity; ISO:MGI.
DR   GO; GO:0005128; F:erythropoietin receptor binding; ISS:UniProtKB.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0030295; F:protein kinase activator activity; IDA:UniProtKB.
DR   GO; GO:0006953; P:acute-phase response; IEA:Ensembl.
DR   GO; GO:0001525; P:angiogenesis; TAS:DFLAT.
DR   GO; GO:0006915; P:apoptotic process; IDA:MGI.
DR   GO; GO:0055008; P:cardiac muscle tissue morphogenesis; TAS:DFLAT.
DR   GO; GO:0071474; P:cellular hyperosmotic response; ISO:MGI.
DR   GO; GO:0007566; P:embryo implantation; IDA:MGI.
DR   GO; GO:0030218; P:erythrocyte differentiation; IDA:MGI.
DR   GO; GO:0043249; P:erythrocyte maturation; IEA:UniProtKB-KW.
DR   GO; GO:0038162; P:erythropoietin-mediated signaling pathway; ISS:UniProtKB.
DR   GO; GO:0003007; P:heart morphogenesis; TAS:DFLAT.
DR   GO; GO:0042541; P:hemoglobin biosynthetic process; IDA:MGI.
DR   GO; GO:0033028; P:myeloid cell apoptotic process; IDA:MGI.
DR   GO; GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
DR   GO; GO:0010523; P:negative regulation of calcium ion transport into cytosol; ISO:MGI.
DR   GO; GO:1902251; P:negative regulation of erythrocyte apoptotic process; ISO:MGI.
DR   GO; GO:1902219; P:negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress; ISO:MGI.
DR   GO; GO:0033033; P:negative regulation of myeloid cell apoptotic process; IDA:MGI.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR   GO; GO:0018105; P:peptidyl-serine phosphorylation; IDA:UniProtKB.
DR   GO; GO:0042104; P:positive regulation of activated T cell proliferation; ISO:MGI.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR   GO; GO:0045893; P:positive regulation of DNA-templated transcription; ISO:MGI.
DR   GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; ISO:MGI.
DR   GO; GO:0045666; P:positive regulation of neuron differentiation; ISO:MGI.
DR   GO; GO:0010976; P:positive regulation of neuron projection development; ISO:MGI.
DR   GO; GO:0045860; P:positive regulation of protein kinase activity; IDA:UniProtKB.
DR   GO; GO:0046579; P:positive regulation of Ras protein signal transduction; ISO:MGI.
DR   GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; ISO:MGI.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:MGI.
DR   GO; GO:0048678; P:response to axon injury; IEA:Ensembl.
DR   GO; GO:0071548; P:response to dexamethasone; IEA:Ensembl.
DR   GO; GO:0051602; P:response to electrical stimulus; IEA:Ensembl.
DR   GO; GO:0043627; P:response to estrogen; IEA:Ensembl.
DR   GO; GO:0055093; P:response to hyperoxia; IEA:Ensembl.
DR   GO; GO:0001666; P:response to hypoxia; IDA:MGI.
DR   GO; GO:0070555; P:response to interleukin-1; IEA:Ensembl.
DR   GO; GO:0032496; P:response to lipopolysaccharide; IEA:Ensembl.
DR   GO; GO:0009651; P:response to salt stress; IEA:Ensembl.
DR   GO; GO:0033574; P:response to testosterone; IEA:Ensembl.
DR   GO; GO:0033189; P:response to vitamin A; IEA:Ensembl.
DR   GO; GO:0060979; P:vasculogenesis involved in coronary vascular morphogenesis; TAS:DFLAT.
DR   GO; GO:0055010; P:ventricular cardiac muscle tissue morphogenesis; TAS:DFLAT.
DR   GO; GO:0061032; P:visceral serous pericardium development; TAS:DFLAT.
DR   Gene3D; 1.20.1250.10; -; 1.
DR   InterPro; IPR009079; 4_helix_cytokine-like_core.
DR   InterPro; IPR019767; EPO/TPO_CS.
DR   InterPro; IPR001323; EPO_TPO.
DR   InterPro; IPR003013; Erythroptn.
DR   PANTHER; PTHR10370; ERYTHROPOIETIN; 1.
DR   PANTHER; PTHR10370:SF0; ERYTHROPOIETIN; 1.
DR   Pfam; PF00758; EPO_TPO; 1.
DR   PIRSF; PIRSF001951; EPO; 1.
DR   PRINTS; PR00272; ERYTHROPTN.
DR   SUPFAM; SSF47266; 4-helical cytokines; 1.
DR   PROSITE; PS00817; EPO_TPO; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Erythrocyte maturation; Glycoprotein; Hormone;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..26
FT   CHAIN           27..192
FT                   /note="Erythropoietin"
FT                   /id="PRO_0000008405"
FT   CARBOHYD        50
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        64
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        109
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000250"
FT   DISULFID        33..187
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   192 AA;  21365 MW;  65F94E214E0DEF2E CRC64;
     MGVPERPTLL LLLSLLLIPL GLPVLCAPPR LICDSRVLER YILEAKEAEN VTMGCAEGPR
     LSENITVPDT KVNFYAWKRM EVEEQAIEVW QGLSLLSEAI LQAQALLANS SQPPETLQLH
     IDKAISGLRS LTSLLRVLGA QKELMSPPDT TPPAPLRTLT VDTFCKLFRV YANFLRGKLK
     LYTGEVCRRG DR
//