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Protein

Erythropoietin

Gene

Epo

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

GO - Molecular functioni

  1. erythropoietin receptor binding Source: DFLAT
  2. protein kinase activator activity Source: UniProtKB

GO - Biological processi

  1. aging Source: Ensembl
  2. angiogenesis Source: DFLAT
  3. apoptotic process Source: MGI
  4. cardiac muscle tissue morphogenesis Source: DFLAT
  5. cellular hyperosmotic response Source: MGI
  6. embryo implantation Source: MGI
  7. erythrocyte differentiation Source: MGI
  8. erythrocyte maturation Source: UniProtKB-KW
  9. heart morphogenesis Source: DFLAT
  10. hemoglobin biosynthetic process Source: MGI
  11. negative regulation of apoptotic process Source: UniProtKB
  12. negative regulation of calcium ion transport into cytosol Source: MGI
  13. negative regulation of cation channel activity Source: MGI
  14. negative regulation of erythrocyte apoptotic process Source: MGI
  15. negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress Source: MGI
  16. negative regulation of myeloid cell apoptotic process Source: MGI
  17. peptidyl-serine phosphorylation Source: UniProtKB
  18. positive regulation of cell proliferation Source: MGI
  19. positive regulation of DNA replication Source: MGI
  20. positive regulation of neuron differentiation Source: Ensembl
  21. positive regulation of protein kinase activity Source: UniProtKB
  22. positive regulation of Ras protein signal transduction Source: MGI
  23. positive regulation of transcription, DNA-templated Source: MGI
  24. positive regulation of tyrosine phosphorylation of Stat5 protein Source: MGI
  25. regulation of transcription from RNA polymerase II promoter Source: MGI
  26. response to axon injury Source: Ensembl
  27. response to electrical stimulus Source: Ensembl
  28. response to estrogen Source: Ensembl
  29. response to hyperoxia Source: Ensembl
  30. response to hypoxia Source: MGI
  31. response to interleukin-1 Source: Ensembl
  32. response to lipopolysaccharide Source: Ensembl
  33. response to salt stress Source: Ensembl
  34. response to testosterone Source: Ensembl
  35. response to vitamin A Source: Ensembl
  36. vasculogenesis involved in coronary vascular morphogenesis Source: DFLAT
  37. ventricular cardiac muscle tissue morphogenesis Source: DFLAT
  38. visceral serous pericardium development Source: DFLAT
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Keywords - Biological processi

Erythrocyte maturation

Enzyme and pathway databases

ReactomeiREACT_199107. Regulation of gene expression by Hypoxia-inducible Factor.
SABIO-RKP07321.

Names & Taxonomyi

Protein namesi
Recommended name:
Erythropoietin
Gene namesi
Name:Epo
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:95407. Epo.

Subcellular locationi

GO - Cellular componenti

  1. cell surface Source: MGI
  2. extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Add
BLAST
Chaini27 – 192166ErythropoietinPRO_0000008405Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 187By similarity
Glycosylationi50 – 501N-linked (GlcNAc...)By similarity
Glycosylationi64 – 641N-linked (GlcNAc...)By similarity
Glycosylationi109 – 1091N-linked (GlcNAc...)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP07321.

PTM databases

PhosphoSiteiP07321.

Expressioni

Tissue specificityi

Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Gene expression databases

BgeeiP07321.
CleanExiMM_EPO.
GenevestigatoriP07321.

Structurei

3D structure databases

ProteinModelPortaliP07321.
SMRiP07321. Positions 27-192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the EPO/TPO family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG43828.
HOGENOMiHOG000052505.
HOVERGENiHBG003978.
InParanoidiP07321.
KOiK05437.
OMAiNVTMGCA.
OrthoDBiEOG7DVDCR.
PhylomeDBiP07321.
TreeFamiTF333413.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR019767. EPO/TPO_CS.
IPR001323. EPO_TPO.
IPR003013. Erythroptn.
[Graphical view]
PANTHERiPTHR10370. PTHR10370. 1 hit.
PfamiPF00758. EPO_TPO. 1 hit.
[Graphical view]
PIRSFiPIRSF001951. EPO. 1 hit.
PRINTSiPR00272. ERYTHROPTN.
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00817. EPO_TPO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07321-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGVPERPTLL LLLSLLLIPL GLPVLCAPPR LICDSRVLER YILEAKEAEN
60 70 80 90 100
VTMGCAEGPR LSENITVPDT KVNFYAWKRM EVEEQAIEVW QGLSLLSEAI
110 120 130 140 150
LQAQALLANS SQPPETLQLH IDKAISGLRS LTSLLRVLGA QKELMSPPDT
160 170 180 190
TPPAPLRTLT VDTFCKLFRV YANFLRGKLK LYTGEVCRRG DR
Length:192
Mass (Da):21,365
Last modified:April 1, 1988 - v1
Checksum:i65F94E214E0DEF2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12482 Genomic DNA. Translation: AAA37568.1.
M12930 Genomic DNA. Translation: AAA37570.1.
AF312033 Genomic DNA. Translation: AAK28825.1.
Y11971 mRNA. Translation: CAA72707.1.
CCDSiCCDS39332.1.
PIRiA24902.
RefSeqiNP_031968.1. NM_007942.2.
UniGeneiMm.349116.

Genome annotation databases

EnsembliENSMUST00000031723; ENSMUSP00000031723; ENSMUSG00000029711.
ENSMUST00000111038; ENSMUSP00000106667; ENSMUSG00000029711.
GeneIDi13856.
KEGGimmu:13856.
UCSCiuc009acm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12482 Genomic DNA. Translation: AAA37568.1.
M12930 Genomic DNA. Translation: AAA37570.1.
AF312033 Genomic DNA. Translation: AAK28825.1.
Y11971 mRNA. Translation: CAA72707.1.
CCDSiCCDS39332.1.
PIRiA24902.
RefSeqiNP_031968.1. NM_007942.2.
UniGeneiMm.349116.

3D structure databases

ProteinModelPortaliP07321.
SMRiP07321. Positions 27-192.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiP07321.

Proteomic databases

PRIDEiP07321.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031723; ENSMUSP00000031723; ENSMUSG00000029711.
ENSMUST00000111038; ENSMUSP00000106667; ENSMUSG00000029711.
GeneIDi13856.
KEGGimmu:13856.
UCSCiuc009acm.1. mouse.

Organism-specific databases

CTDi2056.
MGIiMGI:95407. Epo.

Phylogenomic databases

eggNOGiNOG43828.
HOGENOMiHOG000052505.
HOVERGENiHBG003978.
InParanoidiP07321.
KOiK05437.
OMAiNVTMGCA.
OrthoDBiEOG7DVDCR.
PhylomeDBiP07321.
TreeFamiTF333413.

Enzyme and pathway databases

ReactomeiREACT_199107. Regulation of gene expression by Hypoxia-inducible Factor.
SABIO-RKP07321.

Miscellaneous databases

NextBioi284726.
PROiP07321.
SOURCEiSearch...

Gene expression databases

BgeeiP07321.
CleanExiMM_EPO.
GenevestigatoriP07321.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR019767. EPO/TPO_CS.
IPR001323. EPO_TPO.
IPR003013. Erythroptn.
[Graphical view]
PANTHERiPTHR10370. PTHR10370. 1 hit.
PfamiPF00758. EPO_TPO. 1 hit.
[Graphical view]
PIRSFiPIRSF001951. EPO. 1 hit.
PRINTSiPR00272. ERYTHROPTN.
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00817. EPO_TPO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Murine erythropoietin gene: cloning, expression, and human gene homology."
    Shoemaker C.B., Mitsock L.D.
    Mol. Cell. Biol. 6:849-858(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning, sequencing, and evolutionary analysis of the mouse erythropoietin gene."
    McDonald J.D., Lin F.-K., Goldwasser E.
    Mol. Cell. Biol. 6:842-848(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Comparative analysis of the gene-dense ACHE/TFR2 region on human chromosome 7q22 with the orthologous region on mouse chromosome 5."
    Wilson M.D., Riemer C., Martindale D.W., Schnupf P., Boright A.P., Cheung T.L., Hardy D.M., Schwartz S., Scherer S.W., Tsui L.-C., Miller W., Koop B.F.
    Nucleic Acids Res. 29:1352-1365(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  4. "Abnormal erythropoietin (Epo) gene expression in the murine erythroleukemia IW32 cells results from a rearrangement between the G-protein beta2 subunit gene and the Epo gene."
    Chretien S., Duprez V., Maouche L., Gisselbrecht S., Mayeux P., Lacombe C.
    Oncogene 15:1995-1999(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-52.
    Strain: ICFW.

Entry informationi

Entry nameiEPO_MOUSE
AccessioniPrimary (citable) accession number: P07321
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: February 4, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.