P07321 (EPO_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Erythropoietin

Gene names

Name:

Epo

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
Subcellular location	Secreted.
Tissue specificity	Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
Sequence similarities	Belongs to the EPO/TPO family.

Ontologies

Keywords
Biological process	Erythrocyte maturation
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from electronic annotation. Source: Compara angiogenesis Traceable author statement PubMed 20299672. Source: DFLAT apoptotic process Inferred from direct assay PubMed 17300687. Source: MGI cellular hyperosmotic response Inferred from electronic annotation. Source: Compara embryo implantation Inferred from direct assay PubMed 17300687. Source: MGI erythrocyte differentiation Inferred from direct assay PubMed 15591122. Source: MGI erythrocyte maturation Inferred from electronic annotation. Source: UniProtKB-KW hemoglobin biosynthetic process Inferred from direct assay PubMed 17300687. Source: MGI negative regulation of ion transmembrane transporter activity Inferred from electronic annotation. Source: Compara negative regulation of myeloid cell apoptotic process Inferred from direct assay PubMed 17981124. Source: MGI negative regulation of sodium ion transport Inferred from electronic annotation. Source: Compara peptidyl-serine phosphorylation Inferred from direct assay PubMed 21095239. Source: UniProtKB positive regulation of DNA replication Inferred from electronic annotation. Source: Compara positive regulation of Ras protein signal transduction Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of neuron differentiation Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara positive regulation of tyrosine phosphorylation of Stat5 protein Inferred from electronic annotation. Source: Compara regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 17300687. Source: MGI response to axon injury Inferred from electronic annotation. Source: Compara response to electrical stimulus Inferred from electronic annotation. Source: Compara response to estrogen stimulus Inferred from electronic annotation. Source: Compara response to hyperoxia Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from direct assay PubMed 15016652. Source: MGI response to interleukin-1 Inferred from electronic annotation. Source: Compara response to lipopolysaccharide Inferred from electronic annotation. Source: Compara response to salt stress Inferred from electronic annotation. Source: Compara response to testosterone stimulus Inferred from electronic annotation. Source: Compara response to vitamin A Inferred from electronic annotation. Source: Compara vasculogenesis involved in coronary vascular morphogenesis Traceable author statement PubMed 20299672. Source: DFLAT ventricular cardiac muscle tissue morphogenesis Traceable author statement PubMed 20299672. Source: DFLAT visceral serous pericardium development Traceable author statement PubMed 20299672. Source: DFLAT
Cellular_component	extracellular space Inferred from direct assay PubMed 15016652. Source: MGI
Molecular_function	erythropoietin receptor binding Traceable author statement PubMed 20299672. Source: DFLAT eukaryotic cell surface binding Inferred from electronic annotation. Source: Compara protein kinase activator activity Inferred from direct assay PubMed 21095239. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26
	Chain	27 – 192	166	Erythropoietin		PRO_0000008405
Amino acid modifications
	Glycosylation	50	1	N-linked (GlcNAc...) By similarity
	Glycosylation	64	1	N-linked (GlcNAc...) By similarity
	Glycosylation	109	1	N-linked (GlcNAc...) By similarity
	Disulfide bond	33 ↔ 187		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07321 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 65F94E214E0DEF2E	FASTA	192	21,365
	10 20 30 40 50 60 MGVPERPTLL LLLSLLLIPL GLPVLCAPPR LICDSRVLER YILEAKEAEN VTMGCAEGPR 70 80 90 100 110 120 LSENITVPDT KVNFYAWKRM EVEEQAIEVW QGLSLLSEAI LQAQALLANS SQPPETLQLH 130 140 150 160 170 180 IDKAISGLRS LTSLLRVLGA QKELMSPPDT TPPAPLRTLT VDTFCKLFRV YANFLRGKLK 190 LYTGEVCRRG DR

References

[1]	"Murine erythropoietin gene: cloning, expression, and human gene homology." Shoemaker C.B., Mitsock L.D. Mol. Cell. Biol. 6:849-858(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning, sequencing, and evolutionary analysis of the mouse erythropoietin gene." McDonald J.D., Lin F.-K., Goldwasser E. Mol. Cell. Biol. 6:842-848(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Comparative analysis of the gene-dense ACHE/TFR2 region on human chromosome 7q22 with the orthologous region on mouse chromosome 5." Wilson M.D., Riemer C., Martindale D.W., Schnupf P., Boright A.P., Cheung T.L., Hardy D.M., Schwartz S., Scherer S.W., Tsui L.-C., Miller W., Koop B.F. Nucleic Acids Res. 29:1352-1365(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[4]	"Abnormal erythropoietin (Epo) gene expression in the murine erythroleukemia IW32 cells results from a rearrangement between the G-protein beta2 subunit gene and the Epo gene." Chretien S., Duprez V., Maouche L., Gisselbrecht S., Mayeux P., Lacombe C. Oncogene 15:1995-1999(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-52. Strain: ICFW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M12482 Genomic DNA. Translation: AAA37568.1. M12930 Genomic DNA. Translation: AAA37570.1. AF312033 Genomic DNA. Translation: AAK28825.1. Y11971 mRNA. Translation: CAA72707.1.
IPI	IPI00127648.
PIR	A24902.
RefSeq	NP_031968.1. NM_007942.2.
UniGene	Mm.349116.
3D structure databases
ProteinModelPortal	P07321.
SMR	P07321. Positions 27-192.
ModBase	Search...
PTM databases
PhosphoSite	P07321.
Proteomic databases
PRIDE	P07321.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000031723; ENSMUSP00000031723; ENSMUSG00000029711. ENSMUST00000111038; ENSMUSP00000106667; ENSMUSG00000029711.
GeneID	13856.
KEGG	mmu:13856.
UCSC	uc009acm.1. mouse.
Organism-specific databases
CTD	2056.
MGI	MGI:95407. Epo.
Phylogenomic databases
eggNOG	NOG43828.
HOGENOM	HOG000052505.
HOVERGEN	HBG003978.
InParanoid	P07321.
KO	K05437.
OMA	NVTMGCA.
OrthoDB	EOG41VK4C.
Gene expression databases
ArrayExpress	P07321.
Bgee	P07321.
CleanEx	MM_EPO.
Genevestigator	P07321.
GermOnline	ENSMUSG00000029711. Mus musculus.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR019767. EPO/TPO_CS. IPR001323. EPO_TPO. IPR003013. Erythroptn. [Graphical view]
PANTHER	PTHR10370. PTHR10370. 1 hit.
Pfam	PF00758. EPO_TPO. 1 hit. [Graphical view]
PIRSF	PIRSF001951. EPO. 1 hit.
PRINTS	PR00272. ERYTHROPTN.
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
PROSITE	PS00817. EPO_TPO. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	EPO. mouse.
NextBio	284726.
SOURCE	Search...

Entry information

Entry name

EPO_MOUSE

Accession

Primary (citable) accession number: P07321

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	May 29, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program