P07316 (CRGB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-crystallin B

Alternative name(s):
Gamma-B-crystallin
Gamma-crystallin 1-2

Gene names

Name:	CRYGB
Synonyms:	CRYG2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Crystallins are the dominant structural components of the vertebrate eye lens.
Subunit structure	Monomer By similarity.
Domain	Has a two-domain beta-structure, folded into four very similar Greek key motifs.
Sequence similarities	Belongs to the beta/gamma-crystallin family. Contains 4 beta/gamma crystallin 'Greek key' domains.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Repeat
Molecular function	Eye lens protein
PTM	Oxidation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lens fiber cell morphogenesis Inferred from electronic annotation. Source: Compara visual perception Non-traceable author statement Ref.5. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: Compara
Molecular_function	structural constituent of eye lens Non-traceable author statement Ref.5. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 175

174

Gamma-crystallin B

PRO_0000057586

Regions

Domain

2 – 40

39

Beta/gamma crystallin 'Greek key' 1

Domain

41 – 83

43

Beta/gamma crystallin 'Greek key' 2

Domain

89 – 129

41

Beta/gamma crystallin 'Greek key' 3

Domain

130 – 172

43

Beta/gamma crystallin 'Greek key' 4

Region

84 – 88

5

Connecting peptide

Sites

Site

69

1

Susceptible to oxidation

Site

70

1

Susceptible to oxidation

Natural variations

Natural variant

73

1

S → I. Ref.8

VAR_021140

Natural variant

90

1

R → T.
Corresponds to variant rs2241980 [ dbSNP | Ensembl ].

VAR_029517

Natural variant

111

1

I → L. Ref.1 Ref.2 Ref.4 Ref.8 Ref.9
Corresponds to variant rs796287 [ dbSNP | Ensembl ].

VAR_021141

Secondary structure

1

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175

Helix Strand Turn

Details...

Beta strand

3 – 9

7

Turn

10 – 12

3

Beta strand

13 – 21

9

Turn

27 – 29

3

Beta strand

34 – 48

15

Turn

49 – 51

3

Beta strand

52 – 58

7

Beta strand

60 – 65

6

Helix

66 – 69

4

Beta strand

72 – 74

3

Beta strand

78 – 82

5

Beta strand

90 – 96

7

Turn

97 – 99

3

Beta strand

100 – 108

9

Helix

113 – 117

5

Beta strand

124 – 130

7

Beta strand

132 – 137

6

Turn

138 – 140

3

Beta strand

141 – 147

7

Beta strand

149 – 152

4

Helix

155 – 158

4

Beta strand

161 – 164

4

Beta strand

167 – 170

4

Sequences

	Sequence		Length	Mass (Da)
	P07316 [UniParc]. Last modified November 2, 2010. Version 3. Checksum: E0B605353FC62AFC	FASTA	175	20,908
	10 20 30 40 50 60 MGKITFYEDR AFQGRSYECT TDCPNLQPYF SRCNSIRVES GCWMIYERPN YQGHQYFLRR 70 80 90 100 110 120 GEYPDYQQWM GLSDSIRSCC LIPPHSGAYR MKIYDRDELR GQMSELTDDC ISVQDRFHLT 130 140 150 160 170 EIHSLNVLEG SWILYEMPNY RGRQYLLRPG EYRRFLDWGA PNAKVGSLRR VMDLY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two human gamma-crystallin genes are linked and riddled with Alu-repeats." den Dunnen J.T., Moormann R.J.M., Cremers F.P.M., Schoenmakers J.G.G. Gene 38:197-204(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LEU-111.
[2]	"Nucleotide sequence of the rat gamma-crystallin gene region and comparison with an orthologous human region." den Dunnen J.T., van Neck J.W., Cremers F.P.M., Lubsen N.H., Schoenmakers J.G.G. Gene 78:201-213(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LEU-111.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-111. Tissue: Testis.
[5]	"Homology models of human gamma-crystallins: structural study of the extensive charge network in gamma-crystallins." Salim A., Zaidi Z.H. Biochem. Biophys. Res. Commun. 300:624-630(2003) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING.
[6]	"Prediction of possible sites for posttranslational modifications in human gamma crystallins: effect of glycation on the structure of human gamma-B-crystallin as analyzed by molecular modeling." Salim A., Bano A., Zaidi Z.H. Proteins 53:162-173(2003) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING.
[7]	"Affilin-novel binding molecules based on human gamma-B-crystallin, an all beta-sheet protein." Ebersbach H., Fiedler E., Scheuermann T., Fiedler M., Stubbs M.T., Reimann C., Proetzel G., Rudolph R., Fiedler U. J. Mol. Biol. 372:172-185(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 2-174.
[8]	"Novel mutations in the gamma-crystallin genes cause autosomal dominant congenital cataracts." Santhiya S.T., Shyam Manohar M., Rawlley D., Vijayalakshmi P., Namperumalsamy P., Gopinath P.M., Loester J., Graw J. J. Med. Genet. 39:352-358(2002) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS ILE-73 AND LEU-111.
[9]	"Gamma-D crystallin gene (CRYGD) mutation causes autosomal dominant congenital cerulean cataracts." Nandrot E., Slingsby C., Basak A., Cherif-Chefchaouni M., Benazzouz B., Hajaji Y., Boutayeb S., Gribouval O., Arbogast L., Berraho A., Abitbol M., Hilal L. J. Med. Genet. 40:262-267(2003) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT LEU-111.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M11971, M11970 Genomic DNA. Translation: AAA52113.1.
M19364 Genomic DNA. Translation: AAA52109.1.
AC016697 Genomic DNA. Translation: AAX93219.1.
BC074944 mRNA. Translation: AAH74944.1.
BC074945 mRNA. Translation: AAH74945.1.
BC117384 mRNA. Translation: AAI17385.1.
BC117388 mRNA. Translation: AAI17389.1.

IPI

IPI00217231.

PIR

CYHUG1. A24520.

RefSeq

NP_005201.2. NM_005210.3.

UniGene

Hs.248102.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LEU	model	-	A	1-175	[»]
1MYV	model	-	A	1-175	[»]
1MYX	model	-	A	1-175	[»]
1MYY	model	-	A	1-175	[»]
1MZ1	model	-	A	1-175	[»]
1MZ2	model	-	A	1-175	[»]
1MZ3	model	-	A	1-175	[»]
2JDF	X-ray	1.70	A	2-174	[»]
2JDG	X-ray	2.00	A	8-174	[»]

ProteinModelPortal

P07316.

SMR

P07316. Positions 2-175.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000260988.

PTM databases

PhosphoSite

P07316.

Polymorphism databases

DMDM

311033463.

Proteomic databases

PaxDb

P07316.

PeptideAtlas

P07316.

PRIDE

P07316.

Protocols and materials databases

DNASU

1419.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000260988; ENSP00000260988; ENSG00000182187.

GeneID

1419.

KEGG

hsa:1419.

UCSC

uc002vcp.4. human.

Organism-specific databases

CTD

1419.

GeneCards

GC02M209007.

H-InvDB

HIX0029984.

HGNC

HGNC:2409. CRYGB.

MIM

123670. gene.

neXtProt

NX_P07316.

PharmGKB

PA26916.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG309484.

HOGENOM

HOG000234389.

HOVERGEN

HBG003364.

InParanoid

P07316.

OMA

GEYRRFL.

OrthoDB

EOG4K9BD7.

PhylomeDB

P07316.

Gene expression databases

Bgee

P07316.

CleanEx

HS_CRYGB.

Genevestigator

P07316.

GermOnline

ENSG00000182187. Homo sapiens.

Family and domain databases

InterPro

IPR001064. Beta/gamma_crystallin.
IPR011024. G_crystallin-rel.
[Graphical view]

Pfam

PF00030. Crystall. 2 hits.
[Graphical view]

PRINTS

PR01367. BGCRYSTALLIN.

SMART

SM00247. XTALbg. 2 hits.
[Graphical view]

SUPFAM

SSF49695. G_crystallin_SF. 1 hit.

PROSITE

PS50915. CRYSTALLIN_BETA_GAMMA. 4 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P07316.

GenomeRNAi

1419.

NextBio

5803.

SOURCE

Search...

Entry information

Entry name

CRGB_HUMAN

Accession

Primary (citable) accession number: P07316
Secondary accession number(s): Q17RB5, Q53ST2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	November 2, 2010
Last modified:	April 3, 2013
This is version 125 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.