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P07313

- MYLK2_RABIT

UniProt

P07313 - MYLK2_RABIT

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Protein

Myosin light chain kinase 2, skeletal/cardiac muscle

Gene

MYLK2

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Implicated in the level of global muscle contraction and cardiac function (By similarity). Phosphorylates a specific serine in the N-terminus of a myosin light chain.By similarity

Catalytic activityi

ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei326 – 3261ATPPROSITE-ProRule annotation
Active sitei418 – 4181Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi303 – 3119ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calmodulin binding Source: BHF-UCL
  3. calmodulin-dependent protein kinase activity Source: BHF-UCL
  4. myosin light chain kinase activity Source: BHF-UCL

GO - Biological processi

  1. regulation of muscle filament sliding Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.18. 1749.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain kinase 2, skeletal/cardiac muscle (EC:2.7.11.18)
Short name:
MLCK2
Gene namesi
Name:MYLK2
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

Cytoplasm 1 Publication
Note: Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 608607Myosin light chain kinase 2, skeletal/cardiac musclePRO_0000086410Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP07313.

Interactioni

Subunit structurei

May interact with centrin.By similarity

Structurei

Secondary structure

1
608
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi581 – 59616

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BBMNMR-B578-603[»]
2BBNNMR-B578-603[»]
ProteinModelPortaliP07313.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07313.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini297 – 552256Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni586 – 59813Calmodulin-bindingAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi273 – 2808Poly-Pro

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG080416.
InParanoidiP07313.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07313-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATENGAVEL GIQSLSTDEA SKGAASEESL AAEKDPAPPD PEKGPGPSDT
60 70 80 90 100
KQDPDPSTPK KDANTPAPEK GDVVPAQPSA GGSQGPAGEG GQVEAPAEGS
110 120 130 140 150
AGKPAALPQQ TATAEASEKK PEAEKGPSGH QDPGEPTVGK KVAEGQAAAR
160 170 180 190 200
RGSPAFLHSP SCPAIIASTE KLPAQKPLSE ASELIFEGVP ATPGPTEPGP
210 220 230 240 250
AKAEGGVDLL AESQKEAGEK APGQADQAKV QGDTSRGIEF QAVPSERPRP
260 270 280 290 300
EVGQALCLPA REEDCFQILD DCPPPPAPFP HRIVELRTGN VSSEFSMNSK
310 320 330 340 350
EALGGGKFGA VCTCTEKSTG LKLAAKVIKK QTPKDKEMVM LEIEVMNQLN
360 370 380 390 400
HRNLIQLYAA IETPHEIVLF MEYIEGGELF ERIVDEDYHL TEVDTMVFVR
410 420 430 440 450
QICDGILFMH KMRVLHLDLK PENILCVNTT GHLVKIIDFG LARRYNPNEK
460 470 480 490 500
LKVNFGTPEF LSPEVVNYDQ ISDKTDMWSL GVITYMLLSG LSPFLGDDDT
510 520 530 540 550
ETLNNVLSGN WYFDEETFEA VSDEAKDFVS NLIVKEQGAR MSAAQCLAHP
560 570 580 590 600
WLNNLAEKAK RCNRRLKSQI LLKKYLMKRR WKKNFIAVSA ANRFKKISSS

GALMALGV
Length:608
Mass (Da):65,468
Last modified:January 23, 2007 - v3
Checksum:i9B2FD074165DADFE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti336 – 3361K → KK AA sequence (PubMed:3542042)Curated
Sequence conflicti336 – 3361K → KK AA sequence (PubMed:3841288)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05194 mRNA. Translation: AAA31400.1.
PIRiA35021.
RefSeqiNP_001075705.1. NM_001082236.1.
UniGeneiOcu.1927.

Genome annotation databases

GeneIDi100009051.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05194 mRNA. Translation: AAA31400.1 .
PIRi A35021.
RefSeqi NP_001075705.1. NM_001082236.1.
UniGenei Ocu.1927.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2BBM NMR - B 578-603 [» ]
2BBN NMR - B 578-603 [» ]
ProteinModelPortali P07313.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P07313.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100009051.

Organism-specific databases

CTDi 85366.

Phylogenomic databases

HOVERGENi HBG080416.
InParanoidi P07313.

Enzyme and pathway databases

BRENDAi 2.7.11.18. 1749.

Miscellaneous databases

EvolutionaryTracei P07313.

Family and domain databases

InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR24347. PTHR24347. 1 hit.
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Domain characterization of rabbit skeletal muscle myosin light chain kinase."
    Herring B.P., Stull J.T., Gallagher P.J.
    J. Biol. Chem. 265:1724-1730(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Amino acid sequence of rabbit skeletal muscle myosin light chain kinase."
    Takio K., Blumenthal D.K., Walsh K.A., Titani K., Krebs E.G.
    Biochemistry 25:8049-8057(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-604.
  3. "Amino acid sequence of an active fragment of rabbit skeletal muscle myosin light chain kinase."
    Takio K., Blumenthal D.K., Edelman A.M., Walsh K.A., Krebs E.G., Titani K.
    Biochemistry 24:6028-6037(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 296-604.
  4. "The overall pattern of cardiac contraction depends on a spatial gradient of myosin regulatory light chain phosphorylation."
    Davis J.S., Hassanzadeh S., Winitsky S., Lin H., Satorius C., Vemuri R., Aletras A.H., Wen H., Epstein N.D.
    Cell 107:631-641(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "Solution structure of a calmodulin-target peptide complex by multidimensional NMR."
    Ikura M., Clore G.M., Gronenborn A.M., Zhu G., Klee C.B., Bax A.
    Science 256:632-638(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 578-603.

Entry informationi

Entry nameiMYLK2_RABIT
AccessioniPrimary (citable) accession number: P07313
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3