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Protein

Myosin light chain kinase 2, skeletal/cardiac muscle

Gene

MYLK2

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Implicated in the level of global muscle contraction and cardiac function (By similarity). Phosphorylates a specific serine in the N-terminus of a myosin light chain.By similarity

Catalytic activityi

ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei326ATPPROSITE-ProRule annotation1
Active sitei418Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi303 – 311ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calmodulin binding Source: BHF-UCL
  • calmodulin-dependent protein kinase activity Source: BHF-UCL
  • myosin light chain kinase activity Source: BHF-UCL

GO - Biological processi

  • regulation of muscle filament sliding Source: BHF-UCL

Keywordsi

Molecular functionCalmodulin-binding, Kinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.18. 1749.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain kinase 2, skeletal/cardiac muscle (EC:2.7.11.18)
Short name:
MLCK2
Gene namesi
Name:MYLK2
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000864102 – 608Myosin light chain kinase 2, skeletal/cardiac muscleAdd BLAST607

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei153PhosphoserineBy similarity1
Modified residuei159PhosphoserineBy similarity1
Modified residuei161PhosphoserineBy similarity1
Modified residuei457PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP07313.

PTM databases

iPTMnetiP07313.

Interactioni

Subunit structurei

May interact with centrin.By similarity

GO - Molecular functioni

  • calmodulin binding Source: BHF-UCL

Structurei

Secondary structure

1608
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi581 – 596Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BBMNMR-B578-603[»]
2BBNNMR-B578-603[»]
ProteinModelPortaliP07313.
SMRiP07313.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07313.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini297 – 552Protein kinasePROSITE-ProRule annotationAdd BLAST256

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni586 – 598Calmodulin-bindingAdd BLAST13

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi273 – 280Poly-Pro8

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG080416.
InParanoidiP07313.
KOiK00907.

Family and domain databases

InterProiView protein in InterPro
IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07313-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATENGAVEL GIQSLSTDEA SKGAASEESL AAEKDPAPPD PEKGPGPSDT
60 70 80 90 100
KQDPDPSTPK KDANTPAPEK GDVVPAQPSA GGSQGPAGEG GQVEAPAEGS
110 120 130 140 150
AGKPAALPQQ TATAEASEKK PEAEKGPSGH QDPGEPTVGK KVAEGQAAAR
160 170 180 190 200
RGSPAFLHSP SCPAIIASTE KLPAQKPLSE ASELIFEGVP ATPGPTEPGP
210 220 230 240 250
AKAEGGVDLL AESQKEAGEK APGQADQAKV QGDTSRGIEF QAVPSERPRP
260 270 280 290 300
EVGQALCLPA REEDCFQILD DCPPPPAPFP HRIVELRTGN VSSEFSMNSK
310 320 330 340 350
EALGGGKFGA VCTCTEKSTG LKLAAKVIKK QTPKDKEMVM LEIEVMNQLN
360 370 380 390 400
HRNLIQLYAA IETPHEIVLF MEYIEGGELF ERIVDEDYHL TEVDTMVFVR
410 420 430 440 450
QICDGILFMH KMRVLHLDLK PENILCVNTT GHLVKIIDFG LARRYNPNEK
460 470 480 490 500
LKVNFGTPEF LSPEVVNYDQ ISDKTDMWSL GVITYMLLSG LSPFLGDDDT
510 520 530 540 550
ETLNNVLSGN WYFDEETFEA VSDEAKDFVS NLIVKEQGAR MSAAQCLAHP
560 570 580 590 600
WLNNLAEKAK RCNRRLKSQI LLKKYLMKRR WKKNFIAVSA ANRFKKISSS

GALMALGV
Length:608
Mass (Da):65,468
Last modified:January 23, 2007 - v3
Checksum:i9B2FD074165DADFE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti336K → KK AA sequence (PubMed:3542042).Curated1
Sequence conflicti336K → KK AA sequence (PubMed:3841288).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05194 mRNA. Translation: AAA31400.1.
PIRiA35021.
RefSeqiNP_001075705.1. NM_001082236.1.
UniGeneiOcu.1927.

Genome annotation databases

GeneIDi100009051.
KEGGiocu:100009051.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMYLK2_RABIT
AccessioniPrimary (citable) accession number: P07313
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: February 15, 2017
This is version 133 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families