P07313 (MYLK2_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myosin light chain kinase 2, skeletal/cardiac muscle
Short name=MLCK2
EC=2.7.11.18

Gene names

Name:

MYLK2

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	608 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Implicated in the level of global muscle contraction and cardiac function By similarity. Phosphorylates a specific serine in the N-terminus of a myosin light chain.
Catalytic activity	ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.
Subunit structure	May interact with centrin By similarity.
Subcellular location	Cytoplasm. Note: Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils. Ref.4
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of muscle filament sliding Inferred by curator PubMed 9535879. Source: BHF-UCL
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from direct assay PubMed 8550626. Source: BHF-UCL calmodulin-dependent protein kinase activity Inferred from direct assay PubMed 9535879. Source: BHF-UCL myosin light chain kinase activity Inferred from direct assay PubMed 9535879. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2

Chain

2 – 608

607

Myosin light chain kinase 2, skeletal/cardiac muscle

PRO_0000086410

Regions

Domain

297 – 552

256

Protein kinase

Nucleotide binding

303 – 311

9

ATP By similarity

Region

586 – 598

13

Calmodulin-binding

Compositional bias

273 – 280

8

Poly-Pro

Sites

Active site

418

1

Proton acceptor By similarity

Binding site

326

1

ATP By similarity

Amino acid modifications

Modified residue

2

1

N-acetylalanine

Experimental info

Sequence conflict

336

1

K → KK AA sequence Ref.2

Sequence conflict

336

1

K → KK AA sequence Ref.3

Secondary structure

1

.

.

.

608

Helix Strand Turn

Details...

Helix

581 – 596

16

Sequences

	Sequence		Length	Mass (Da)
	P07313 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 9B2FD074165DADFE	FASTA	608	65,468
	10 20 30 40 50 60 MATENGAVEL GIQSLSTDEA SKGAASEESL AAEKDPAPPD PEKGPGPSDT KQDPDPSTPK 70 80 90 100 110 120 KDANTPAPEK GDVVPAQPSA GGSQGPAGEG GQVEAPAEGS AGKPAALPQQ TATAEASEKK 130 140 150 160 170 180 PEAEKGPSGH QDPGEPTVGK KVAEGQAAAR RGSPAFLHSP SCPAIIASTE KLPAQKPLSE 190 200 210 220 230 240 ASELIFEGVP ATPGPTEPGP AKAEGGVDLL AESQKEAGEK APGQADQAKV QGDTSRGIEF 250 260 270 280 290 300 QAVPSERPRP EVGQALCLPA REEDCFQILD DCPPPPAPFP HRIVELRTGN VSSEFSMNSK 310 320 330 340 350 360 EALGGGKFGA VCTCTEKSTG LKLAAKVIKK QTPKDKEMVM LEIEVMNQLN HRNLIQLYAA 370 380 390 400 410 420 IETPHEIVLF MEYIEGGELF ERIVDEDYHL TEVDTMVFVR QICDGILFMH KMRVLHLDLK 430 440 450 460 470 480 PENILCVNTT GHLVKIIDFG LARRYNPNEK LKVNFGTPEF LSPEVVNYDQ ISDKTDMWSL 490 500 510 520 530 540 GVITYMLLSG LSPFLGDDDT ETLNNVLSGN WYFDEETFEA VSDEAKDFVS NLIVKEQGAR 550 560 570 580 590 600 MSAAQCLAHP WLNNLAEKAK RCNRRLKSQI LLKKYLMKRR WKKNFIAVSA ANRFKKISSS GALMALGV

References

[1]	"Domain characterization of rabbit skeletal muscle myosin light chain kinase." Herring B.P., Stull J.T., Gallagher P.J. J. Biol. Chem. 265:1724-1730(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Amino acid sequence of rabbit skeletal muscle myosin light chain kinase." Takio K., Blumenthal D.K., Walsh K.A., Titani K., Krebs E.G. Biochemistry 25:8049-8057(1986) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-604.
[3]	"Amino acid sequence of an active fragment of rabbit skeletal muscle myosin light chain kinase." Takio K., Blumenthal D.K., Edelman A.M., Walsh K.A., Krebs E.G., Titani K. Biochemistry 24:6028-6037(1985) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 296-604.
[4]	"The overall pattern of cardiac contraction depends on a spatial gradient of myosin regulatory light chain phosphorylation." Davis J.S., Hassanzadeh S., Winitsky S., Lin H., Satorius C., Vemuri R., Aletras A.H., Wen H., Epstein N.D. Cell 107:631-641(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Solution structure of a calmodulin-target peptide complex by multidimensional NMR." Ikura M., Clore G.M., Gronenborn A.M., Zhu G., Klee C.B., Bax A. Science 256:632-638(1992) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 578-603.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J05194 mRNA. Translation: AAA31400.1.

PIR

A35021.

RefSeq

NP_001075705.1. NM_001082236.1.

UniGene

Ocu.1927.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BBM	NMR	-	B	578-603	[»]
2BBN	NMR	-	B	578-603	[»]

ProteinModelPortal

P07313.

ModBase

Search...

Proteomic databases

PRIDE

P07313.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

100009051.

Organism-specific databases

CTD

85366.

Phylogenomic databases

HOVERGEN

HBG080416.

Enzyme and pathway databases

BRENDA

2.7.11.18. 1749.

Family and domain databases

InterPro

IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]

PANTHER

PTHR24347. PTHR24347. 1 hit.

Pfam

PF00069. Pkinase. 1 hit.
[Graphical view]

SMART

SM00220. S_TKc. 1 hit.
[Graphical view]

SUPFAM

SSF56112. Kinase_like. 1 hit.

PROSITE

PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P07313.

Entry information

Entry name

MYLK2_RABIT

Accession

Primary (citable) accession number: P07313

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 119 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program