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P07313

- MYLK2_RABIT

UniProt

P07313 - MYLK2_RABIT

Protein

Myosin light chain kinase 2, skeletal/cardiac muscle

Gene

MYLK2

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Implicated in the level of global muscle contraction and cardiac function By similarity. Phosphorylates a specific serine in the N-terminus of a myosin light chain.By similarity

    Catalytic activityi

    ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei326 – 3261ATPPROSITE-ProRule annotation
    Active sitei418 – 4181Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi303 – 3119ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calmodulin binding Source: BHF-UCL
    3. calmodulin-dependent protein kinase activity Source: BHF-UCL
    4. myosin light chain kinase activity Source: BHF-UCL

    GO - Biological processi

    1. regulation of muscle filament sliding Source: BHF-UCL

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calmodulin-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.18. 1749.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin light chain kinase 2, skeletal/cardiac muscle (EC:2.7.11.18)
    Short name:
    MLCK2
    Gene namesi
    Name:MYLK2
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    Cytoplasm 1 Publication
    Note: Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 608607Myosin light chain kinase 2, skeletal/cardiac musclePRO_0000086410Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiP07313.

    Interactioni

    Subunit structurei

    May interact with centrin.By similarity

    Structurei

    Secondary structure

    1
    608
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi581 – 59616

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2BBMNMR-B578-603[»]
    2BBNNMR-B578-603[»]
    ProteinModelPortaliP07313.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07313.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini297 – 552256Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni586 – 59813Calmodulin-bindingAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi273 – 2808Poly-Pro

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG080416.

    Family and domain databases

    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07313-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATENGAVEL GIQSLSTDEA SKGAASEESL AAEKDPAPPD PEKGPGPSDT    50
    KQDPDPSTPK KDANTPAPEK GDVVPAQPSA GGSQGPAGEG GQVEAPAEGS 100
    AGKPAALPQQ TATAEASEKK PEAEKGPSGH QDPGEPTVGK KVAEGQAAAR 150
    RGSPAFLHSP SCPAIIASTE KLPAQKPLSE ASELIFEGVP ATPGPTEPGP 200
    AKAEGGVDLL AESQKEAGEK APGQADQAKV QGDTSRGIEF QAVPSERPRP 250
    EVGQALCLPA REEDCFQILD DCPPPPAPFP HRIVELRTGN VSSEFSMNSK 300
    EALGGGKFGA VCTCTEKSTG LKLAAKVIKK QTPKDKEMVM LEIEVMNQLN 350
    HRNLIQLYAA IETPHEIVLF MEYIEGGELF ERIVDEDYHL TEVDTMVFVR 400
    QICDGILFMH KMRVLHLDLK PENILCVNTT GHLVKIIDFG LARRYNPNEK 450
    LKVNFGTPEF LSPEVVNYDQ ISDKTDMWSL GVITYMLLSG LSPFLGDDDT 500
    ETLNNVLSGN WYFDEETFEA VSDEAKDFVS NLIVKEQGAR MSAAQCLAHP 550
    WLNNLAEKAK RCNRRLKSQI LLKKYLMKRR WKKNFIAVSA ANRFKKISSS 600
    GALMALGV 608
    Length:608
    Mass (Da):65,468
    Last modified:January 23, 2007 - v3
    Checksum:i9B2FD074165DADFE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti336 – 3361K → KK AA sequence (PubMed:3542042)Curated
    Sequence conflicti336 – 3361K → KK AA sequence (PubMed:3841288)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05194 mRNA. Translation: AAA31400.1.
    PIRiA35021.
    RefSeqiNP_001075705.1. NM_001082236.1.
    UniGeneiOcu.1927.

    Genome annotation databases

    GeneIDi100009051.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05194 mRNA. Translation: AAA31400.1 .
    PIRi A35021.
    RefSeqi NP_001075705.1. NM_001082236.1.
    UniGenei Ocu.1927.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2BBM NMR - B 578-603 [» ]
    2BBN NMR - B 578-603 [» ]
    ProteinModelPortali P07313.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P07313.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100009051.

    Organism-specific databases

    CTDi 85366.

    Phylogenomic databases

    HOVERGENi HBG080416.

    Enzyme and pathway databases

    BRENDAi 2.7.11.18. 1749.

    Miscellaneous databases

    EvolutionaryTracei P07313.

    Family and domain databases

    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Domain characterization of rabbit skeletal muscle myosin light chain kinase."
      Herring B.P., Stull J.T., Gallagher P.J.
      J. Biol. Chem. 265:1724-1730(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Amino acid sequence of rabbit skeletal muscle myosin light chain kinase."
      Takio K., Blumenthal D.K., Walsh K.A., Titani K., Krebs E.G.
      Biochemistry 25:8049-8057(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-604.
    3. "Amino acid sequence of an active fragment of rabbit skeletal muscle myosin light chain kinase."
      Takio K., Blumenthal D.K., Edelman A.M., Walsh K.A., Krebs E.G., Titani K.
      Biochemistry 24:6028-6037(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 296-604.
    4. "The overall pattern of cardiac contraction depends on a spatial gradient of myosin regulatory light chain phosphorylation."
      Davis J.S., Hassanzadeh S., Winitsky S., Lin H., Satorius C., Vemuri R., Aletras A.H., Wen H., Epstein N.D.
      Cell 107:631-641(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. "Solution structure of a calmodulin-target peptide complex by multidimensional NMR."
      Ikura M., Clore G.M., Gronenborn A.M., Zhu G., Klee C.B., Bax A.
      Science 256:632-638(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 578-603.

    Entry informationi

    Entry nameiMYLK2_RABIT
    AccessioniPrimary (citable) accession number: P07313
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 122 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3