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P07313

- MYLK2_RABIT

UniProt

P07313 - MYLK2_RABIT

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Protein
Myosin light chain kinase 2, skeletal/cardiac muscle
Gene
MYLK2
Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Implicated in the level of global muscle contraction and cardiac function By similarity. Phosphorylates a specific serine in the N-terminus of a myosin light chain.

Catalytic activityi

ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei326 – 3261ATP By similarity
Active sitei418 – 4181Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi303 – 3119ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calmodulin binding Source: BHF-UCL
  3. calmodulin-dependent protein kinase activity Source: BHF-UCL
  4. myosin light chain kinase activity Source: BHF-UCL

GO - Biological processi

  1. regulation of muscle filament sliding Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.18. 1749.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain kinase 2, skeletal/cardiac muscle (EC:2.7.11.18)
Short name:
MLCK2
Gene namesi
Name:MYLK2
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

Cytoplasm
Note: Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 608607Myosin light chain kinase 2, skeletal/cardiac muscle
PRO_0000086410Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP07313.

Interactioni

Subunit structurei

May interact with centrin By similarity.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi581 – 59616

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BBMNMR-B578-603[»]
2BBNNMR-B578-603[»]
ProteinModelPortaliP07313.

Miscellaneous databases

EvolutionaryTraceiP07313.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini297 – 552256Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni586 – 59813Calmodulin-binding
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi273 – 2808Poly-Pro

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG080416.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07313-1 [UniParc]FASTAAdd to Basket

« Hide

MATENGAVEL GIQSLSTDEA SKGAASEESL AAEKDPAPPD PEKGPGPSDT    50
KQDPDPSTPK KDANTPAPEK GDVVPAQPSA GGSQGPAGEG GQVEAPAEGS 100
AGKPAALPQQ TATAEASEKK PEAEKGPSGH QDPGEPTVGK KVAEGQAAAR 150
RGSPAFLHSP SCPAIIASTE KLPAQKPLSE ASELIFEGVP ATPGPTEPGP 200
AKAEGGVDLL AESQKEAGEK APGQADQAKV QGDTSRGIEF QAVPSERPRP 250
EVGQALCLPA REEDCFQILD DCPPPPAPFP HRIVELRTGN VSSEFSMNSK 300
EALGGGKFGA VCTCTEKSTG LKLAAKVIKK QTPKDKEMVM LEIEVMNQLN 350
HRNLIQLYAA IETPHEIVLF MEYIEGGELF ERIVDEDYHL TEVDTMVFVR 400
QICDGILFMH KMRVLHLDLK PENILCVNTT GHLVKIIDFG LARRYNPNEK 450
LKVNFGTPEF LSPEVVNYDQ ISDKTDMWSL GVITYMLLSG LSPFLGDDDT 500
ETLNNVLSGN WYFDEETFEA VSDEAKDFVS NLIVKEQGAR MSAAQCLAHP 550
WLNNLAEKAK RCNRRLKSQI LLKKYLMKRR WKKNFIAVSA ANRFKKISSS 600
GALMALGV 608
Length:608
Mass (Da):65,468
Last modified:January 23, 2007 - v3
Checksum:i9B2FD074165DADFE
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti336 – 3361K → KK AA sequence 1 Publication
Sequence conflicti336 – 3361K → KK AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05194 mRNA. Translation: AAA31400.1.
PIRiA35021.
RefSeqiNP_001075705.1. NM_001082236.1.
UniGeneiOcu.1927.

Genome annotation databases

GeneIDi100009051.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05194 mRNA. Translation: AAA31400.1 .
PIRi A35021.
RefSeqi NP_001075705.1. NM_001082236.1.
UniGenei Ocu.1927.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2BBM NMR - B 578-603 [» ]
2BBN NMR - B 578-603 [» ]
ProteinModelPortali P07313.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P07313.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100009051.

Organism-specific databases

CTDi 85366.

Phylogenomic databases

HOVERGENi HBG080416.

Enzyme and pathway databases

BRENDAi 2.7.11.18. 1749.

Miscellaneous databases

EvolutionaryTracei P07313.

Family and domain databases

InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR24347. PTHR24347. 1 hit.
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Domain characterization of rabbit skeletal muscle myosin light chain kinase."
    Herring B.P., Stull J.T., Gallagher P.J.
    J. Biol. Chem. 265:1724-1730(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Amino acid sequence of rabbit skeletal muscle myosin light chain kinase."
    Takio K., Blumenthal D.K., Walsh K.A., Titani K., Krebs E.G.
    Biochemistry 25:8049-8057(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-604.
  3. "Amino acid sequence of an active fragment of rabbit skeletal muscle myosin light chain kinase."
    Takio K., Blumenthal D.K., Edelman A.M., Walsh K.A., Krebs E.G., Titani K.
    Biochemistry 24:6028-6037(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 296-604.
  4. "The overall pattern of cardiac contraction depends on a spatial gradient of myosin regulatory light chain phosphorylation."
    Davis J.S., Hassanzadeh S., Winitsky S., Lin H., Satorius C., Vemuri R., Aletras A.H., Wen H., Epstein N.D.
    Cell 107:631-641(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "Solution structure of a calmodulin-target peptide complex by multidimensional NMR."
    Ikura M., Clore G.M., Gronenborn A.M., Zhu G., Klee C.B., Bax A.
    Science 256:632-638(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 578-603.

Entry informationi

Entry nameiMYLK2_RABIT
AccessioniPrimary (citable) accession number: P07313
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: October 16, 2013
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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