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P07313 (MYLK2_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myosin light chain kinase 2, skeletal/cardiac muscle

Short name=MLCK2
EC=2.7.11.18
Gene names
Name:MYLK2
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus

Protein attributes

Sequence length608 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Implicated in the level of global muscle contraction and cardiac function By similarity. Phosphorylates a specific serine in the N-terminus of a myosin light chain.

Catalytic activity

ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.

Subunit structure

May interact with centrin By similarity.

Subcellular location

Cytoplasm. Note: Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils. Ref.4

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 608607Myosin light chain kinase 2, skeletal/cardiac muscle
PRO_0000086410

Regions

Domain297 – 552256Protein kinase
Nucleotide binding303 – 3119ATP By similarity
Region586 – 59813Calmodulin-binding
Compositional bias273 – 2808Poly-Pro

Sites

Active site4181Proton acceptor By similarity
Binding site3261ATP By similarity

Amino acid modifications

Modified residue21N-acetylalanine

Experimental info

Sequence conflict3361K → KK AA sequence Ref.2
Sequence conflict3361K → KK AA sequence Ref.3

Secondary structure

... 608
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07313 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 9B2FD074165DADFE

FASTA60865,468
        10         20         30         40         50         60 
MATENGAVEL GIQSLSTDEA SKGAASEESL AAEKDPAPPD PEKGPGPSDT KQDPDPSTPK 

        70         80         90        100        110        120 
KDANTPAPEK GDVVPAQPSA GGSQGPAGEG GQVEAPAEGS AGKPAALPQQ TATAEASEKK 

       130        140        150        160        170        180 
PEAEKGPSGH QDPGEPTVGK KVAEGQAAAR RGSPAFLHSP SCPAIIASTE KLPAQKPLSE 

       190        200        210        220        230        240 
ASELIFEGVP ATPGPTEPGP AKAEGGVDLL AESQKEAGEK APGQADQAKV QGDTSRGIEF 

       250        260        270        280        290        300 
QAVPSERPRP EVGQALCLPA REEDCFQILD DCPPPPAPFP HRIVELRTGN VSSEFSMNSK 

       310        320        330        340        350        360 
EALGGGKFGA VCTCTEKSTG LKLAAKVIKK QTPKDKEMVM LEIEVMNQLN HRNLIQLYAA 

       370        380        390        400        410        420 
IETPHEIVLF MEYIEGGELF ERIVDEDYHL TEVDTMVFVR QICDGILFMH KMRVLHLDLK 

       430        440        450        460        470        480 
PENILCVNTT GHLVKIIDFG LARRYNPNEK LKVNFGTPEF LSPEVVNYDQ ISDKTDMWSL 

       490        500        510        520        530        540 
GVITYMLLSG LSPFLGDDDT ETLNNVLSGN WYFDEETFEA VSDEAKDFVS NLIVKEQGAR 

       550        560        570        580        590        600 
MSAAQCLAHP WLNNLAEKAK RCNRRLKSQI LLKKYLMKRR WKKNFIAVSA ANRFKKISSS 


GALMALGV 

« Hide

References

[1]"Domain characterization of rabbit skeletal muscle myosin light chain kinase."
Herring B.P., Stull J.T., Gallagher P.J.
J. Biol. Chem. 265:1724-1730(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Amino acid sequence of rabbit skeletal muscle myosin light chain kinase."
Takio K., Blumenthal D.K., Walsh K.A., Titani K., Krebs E.G.
Biochemistry 25:8049-8057(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-604.
[3]"Amino acid sequence of an active fragment of rabbit skeletal muscle myosin light chain kinase."
Takio K., Blumenthal D.K., Edelman A.M., Walsh K.A., Krebs E.G., Titani K.
Biochemistry 24:6028-6037(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 296-604.
[4]"The overall pattern of cardiac contraction depends on a spatial gradient of myosin regulatory light chain phosphorylation."
Davis J.S., Hassanzadeh S., Winitsky S., Lin H., Satorius C., Vemuri R., Aletras A.H., Wen H., Epstein N.D.
Cell 107:631-641(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"Solution structure of a calmodulin-target peptide complex by multidimensional NMR."
Ikura M., Clore G.M., Gronenborn A.M., Zhu G., Klee C.B., Bax A.
Science 256:632-638(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 578-603.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J05194 mRNA. Translation: AAA31400.1.
PIRA35021.
RefSeqNP_001075705.1. NM_001082236.1.
UniGeneOcu.1927.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2BBMNMR-B578-603[»]
2BBNNMR-B578-603[»]
ProteinModelPortalP07313.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP07313.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100009051.

Organism-specific databases

CTD85366.

Phylogenomic databases

HOVERGENHBG080416.

Enzyme and pathway databases

BRENDA2.7.11.18. 1749.

Family and domain databases

InterProIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERPTHR24347. PTHR24347. 1 hit.
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP07313.

Entry information

Entry nameMYLK2_RABIT
AccessionPrimary (citable) accession number: P07313
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: October 16, 2013
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references