Reviewed, UniProtKB/Swiss-Prot P07310 (KCRM_MOUSE)

Names and origin

Protein names

Recommended name:
    Creatine kinase M-type
    EC=2.7.3.2
Alternative name(s):
    Creatine kinase M chain
    M-CK

Gene names

Name:	Ckm
Synonyms:	Ckmm

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Catalytic activity	ATP + creatine = ADP + phosphocreatine.
Subunit structure	Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the ATP:guanido phosphotransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	phosphocreatine biosynthetic process Inferred from direct assay. Source: MGI
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW creatine kinase activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Creatine kinase M-type		PRO_0000211976
Regions
	Nucleotide binding	128 – 132	5	ATP By similarity
	Nucleotide binding	320 – 325	6	ATP By similarity
Sites
	Binding site	191	1	ATP By similarity
	Binding site	236	1	ATP By similarity
	Binding site	292	1	ATP By similarity
	Binding site	335	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07310-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: B573FEB1D2A41056	FASTA	381	43,045
	10 20 30 40 50 60 MPFGNTHNKF KLNYKPQEEY PDLSKHNNHM AKVLTPDLYN KLRDKETPSG FTLDDVIQTG 70 80 90 100 110 120 VDNPGHPFIM TVGCVAGDEE SYTVFKDLFD PIIQDRHGGY KPTDKHKTDL NHENLKGGDD 130 140 150 160 170 180 LDPNYVLSSR VRTGRSIKGY TLPPHCSRGE RRAVEKLSVE ALNSLTGEFK GKYYPLKSMT 190 200 210 220 230 240 EQEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKSFLVWVN EEDHLRVISM 250 260 270 280 290 300 EKGGNMKEVF RRFCVGLQKI EEIFKKAGHP FMWNEHLGYV LTCPSNLGTG LRGGVHVKLA 310 320 330 340 350 360 NLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GAVFDISNAD RLGSSEVEQV QLVVDGVKLM 370 380 VEMEKKLEKG QSIDDMIPAQ K

References

[1]	"The mouse muscle creatine kinase cDNA and deduced amino acid sequences: comparison to evolutionarily related enzymes." Buskin J.N., Jaynes J.B., Chamberlain J.S., Hauschka S.D. J. Mol. Evol. 22:334-341(1985) [PubMed: 3003372] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X03233 mRNA. Translation: CAA26979.1.
IPI	IPI00127596.
PIR	A23590.
RefSeq	NP_031736.1.
UniGene	Mm.2375
3D structure databases
HSSP	HSSP built from PDB template 2CRK based on UniProtKB P00563.
SMR	P07310. Positions 2-381.
ModBase	Search...
Protein-protein interaction databases
STRING	P07310.
PTM databases
PhosphoSite	P07310.
2-D gel databases
SWISS-2DPAGE	P07310.
Proteomic databases
PRIDE	P07310.
Genome annotation databases
Ensembl	ENSMUST00000003643; ENSMUSP00000003643; ENSMUSG00000030399; Mus musculus. [Genome view]
GeneID	12715.
KEGG	mmu:12715.
UCSC	uc009flu.1. mouse.
Organism-specific databases
CTD	12715.
MGI	MGI:88413. Ckm.
Phylogenomic databases
HOGENOM	P07310.
HOVERGEN	P07310.
OMA	TVFKDLF.
Enzyme and pathway databases
BRENDA	2.7.3.2. 244.
Gene expression databases
ArrayExpress	P07310.
Bgee	P07310.
CleanEx	MM_CKM.
Genevestigator	P07310.
GermOnline	ENSMUSG00000030399. Mus musculus.
Family and domain databases
InterPro	IPR000749. ATP-guanido_PTrfase. IPR014746. Gln_synth/guanido_kin_cat. [Graphical view]
Gene3D	G3DSA:1.10.135.10. ATP-gua_Ptrans. 1 hit. G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
PANTHER	PTHR11547. ATP-gua_Ptrans. 1 hit.
Pfam	PF00217. ATP-gua_Ptrans. 1 hit. PF02807. ATP-gua_PtransN. 1 hit. [Graphical view]
PROSITE	PS00112. GUANIDO_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	281984.
SOURCE	Search...

Entry information

Entry name

KCRM_MOUSE

Accession

Primary (citable) accession number: P07310

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	November 3, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)