P07309 (TTHY_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transthyretin

Alternative name(s):
Prealbumin

Gene names

Name:

Ttr

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
Subunit structure	Homotetramer. Dimer of dimers. In the homotetramer, subunits assemble around a central channel that can accommodate two ligand molecules. Interacts with RBP4 By similarity. Ref.7
Subcellular location	Secreted.
Tissue specificity	Detected in plasma (at protein level). Detected in liver.
Miscellaneous	The mouse protein shows increased stability and much reduced propensity to form amyloid fibrils, compared to the human protein.
Sequence similarities	Belongs to the transthyretin family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone Thyroid hormone
PTM	Gamma-carboxyglutamic acid Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	retinol metabolic process Inferred from electronic annotation. Source: Compara transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: Compara protein complex Inferred from electronic annotation. Source: Compara
Molecular_function	hormone binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 20

20

Chain

21 – 147

127

Transthyretin

PRO_0000035760

Regions

Region

135 – 139

5

Thyroid hormone binding By similarity

Sites

Binding site

35

1

Thyroid hormone By similarity

Binding site

74

1

Thyroid hormone By similarity

Amino acid modifications

Modified residue

62

1

4-carboxyglutamate By similarity

Glycosylation

118

1

N-linked (GlcNAc...) Ref.6

Secondary structure

1

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147

Helix Strand Turn

Details...

Beta strand

32 – 38

7

Turn

39 – 42

4

Beta strand

49 – 55

7

Beta strand

61 – 68

8

Beta strand

73 – 75

3

Turn

81 – 83

3

Beta strand

86 – 93

8

Helix

95 – 101

7

Beta strand

107 – 109

3

Beta strand

111 – 118

8

Beta strand

124 – 132

9

Beta strand

135 – 143

9

Sequences

	Sequence		Length	Mass (Da)
	P07309 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 9803CCC3024BA911	FASTA	147	15,776
	10 20 30 40 50 60 MASLRLFLLC LAGLVFVSEA GPAGAGESKC PLMVKVLDAV RGSPAVDVAV KVFKKTSEGS 70 80 90 100 110 120 WEPFASGKTA ESGELHGLTT DEKFVEGVYR VELDTKSYWK TLGISPFHEF ADVVFTANDS 130 140 GHRHYTIAAL LSPYSYSTTA VVSNPQN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural comparisons between mouse and human prealbumin." Wakasugi S., Maeda S., Shimada K., Nakashima H., Migita S. J. Biochem. 98:1707-1714(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], THYROID HORMONE-BINDING SITES LYS-35 AND GLU-74. Tissue: Liver.
[2]	"Structure and expression of the mouse prealbumin gene." Wakasugi S., Maeda S., Shimada K. J. Biochem. 100:49-58(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	Kita H., Kawamoto S., Okubo K., Matsubara K. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: C57BL/6. Tissue: Choroid plexus.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Kidney.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Salivary gland.
[6]	"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-118, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Plasma.
[7]	"Human-murine transthyretin heterotetramers are kinetically stable and non-amyloidogenic. A lesson in the generation of transgenic models of diseases involving oligomeric proteins." Reixach N., Foss T.R., Santelli E., Pascual J., Kelly J.W., Buxbaum J.N. J. Biol. Chem. 283:2098-2107(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 21-147, INCREASED STABILITY OF THE MOUSE PROTEIN, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X03351 mRNA. Translation: CAA27057.1.
D00073 Genomic DNA. Translation: BAA00050.1.
D89076 mRNA. Translation: BAA13757.1.
AK018701 mRNA. Translation: BAB31352.1.
BC024702 mRNA. Translation: AAH24702.1.

IPI

IPI00127560.

PIR

VBMS. A24132.

RefSeq

NP_038725.1. NM_013697.5.

UniGene

Mm.2108.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2QPF	X-ray	2.05	A/B/C/D/E/F/G/H	21-147	[»]

ProteinModelPortal

P07309.

SMR

P07309. Positions 31-144.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29726N.

PTM databases

PhosphoSite

P07309.

2D gel databases

UCD-2DPAGE

P07309.

Proteomic databases

PaxDb

P07309.

PRIDE

P07309.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000075312; ENSMUSP00000074783; ENSMUSG00000061808.

GeneID

22139.

KEGG

mmu:22139.

Organism-specific databases

CTD

7276.

MGI

MGI:98865. Ttr.

Phylogenomic databases

eggNOG

COG2351.

HOGENOM

HOG000251776.

HOVERGEN

HBG000285.

InParanoid

P07309.

OMA

EHAEVSI.

OrthoDB

EOG4B5P6N.

Gene expression databases

ArrayExpress

P07309.

Bgee

P07309.

CleanEx

MM_TTR.

Genevestigator

P07309.

GermOnline

ENSMUSG00000061808. Mus musculus.

Family and domain databases

Gene3D

2.60.40.180. 1 hit.

InterPro

IPR023418. Thyroxine_BS.
IPR000895. Transthyretin/HIU_hydrolase.
IPR023416. Transthyretin/HIU_hydrolase_SF.
IPR023419. Transthyretin_CS.
[Graphical view]

Pfam

PF00576. Transthyretin. 1 hit.
[Graphical view]

PRINTS

PR00189. TRNSTHYRETIN.

SMART

SM00095. TR_THY. 1 hit.
[Graphical view]

SUPFAM

SSF49472. Transthyretin. 1 hit.

PROSITE

PS00768. TRANSTHYRETIN_1. 1 hit.
PS00769. TRANSTHYRETIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

TTR. mouse.

EvolutionaryTrace

P07309.

NextBio

302028.

SOURCE

Search...

Entry information

Entry name

TTHY_MOUSE

Accession

Primary (citable) accession number: P07309

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	April 3, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program