P07307 (ASGR2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asialoglycoprotein receptor 2
Short name=ASGP-R 2
Short name=ASGPR 2

Alternative name(s):
C-type lectin domain family 4 member H2
Hepatic lectin H2
Short name=HL-2

Gene names

Name:	ASGR2
Synonyms:	CLEC4H2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
Subunit structure	The functioning ligand-binding unit of this receptor is thought to be at least a dimer. Interacts with LASS2. Ref.6
Subcellular location	Membrane; Single-pass type II membrane protein.
Tissue specificity	Expressed exclusively in hepatic parenchymal cells.
Miscellaneous	Calcium is required for ligand binding.
Sequence similarities	Contains 1 C-type lectin domain.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Calcium Lectin
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	bone mineralization Inferred from electronic annotation. Source: Compara cell surface receptor signaling pathway Traceable author statement Ref.1. Source: ProtInc glycoprotein metabolic process Inferred from electronic annotation. Source: Compara lipid homeostasis Inferred from electronic annotation. Source: Compara regulation of protein stability Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	asialoglycoprotein receptor activity Traceable author statement Ref.1. Source: UniProtKB carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CERS2	Q96G23	3	EBI-1172636,EBI-1057080

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P07307-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P07307-2)

The sequence of this isoform differs from the canonical sequence as follows:
     24-42: Missing.

Isoform 3 (identifier: P07307-3)

The sequence of this isoform differs from the canonical sequence as follows:
     24-42: Missing.
     82-86: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Asialoglycoprotein receptor 2		PRO_0000046654
Regions
	Topological domain	1 – 58	58	Cytoplasmic Potential
	Transmembrane	59 – 79	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	80 – 311	232	Extracellular Potential
	Domain	176 – 302	127	C-type lectin
	Motif	5 – 8	4	Endocytosis signal Potential
Amino acid modifications
	Modified residue	13	1	Phosphoserine By similarity
	Lipidation	54	1	S-palmitoyl cysteine By similarity
	Glycosylation	102	1	N-linked (GlcNAc...) Ref.7 Ref.8
	Glycosylation	170	1	N-linked (GlcNAc...) Ref.8
	Glycosylation	305	1	N-linked (GlcNAc...)
	Disulfide bond	177 ↔ 188		By similarity
	Disulfide bond	205 ↔ 300		By similarity
	Disulfide bond	278 ↔ 292		By similarity
Natural variations
	Alternative sequence	24 – 42	19	Missing in isoform 2 and isoform 3.		VSP_003060
	Alternative sequence	82 – 86	5	Missing in isoform 3.		VSP_003061
	Natural variant	85	1	G → R. Ref.1 Ref.2 Ref.4 Corresponds to variant rs2304978 [ dbSNP | Ensembl ].		VAR_068747

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: C86D2247FFF604DC	FASTA	311	35,092
	10 20 30 40 50 60 MAKDFQDIQQ LSSEENDHPF HQGEGPGTRR LNPRRGNPFL KGPPPAQPLA QRLCSMVCFS 70 80 90 100 110 120 LLALSFNILL LVVICVTGSQ SEGHGGAQLQ AELRSLKEAF SNFSSSTLTE VQAISTHGGS 130 140 150 160 170 180 VGDKITSLGA KLEKQQQDLK ADHDALLFHL KHFPVDLRFV ACQMELLHSN GSQRTCCPVN 190 200 210 220 230 240 WVEHQGSCYW FSHSGKAWAE AEKYCQLENA HLVVINSWEE QKFIVQHTNP FNTWIGLTDS 250 260 270 280 290 300 DGSWKWVDGT DYRHNYKNWA VTQPDNWHGH ELGGSEDCVE VQPDGRWNDD FCLQVYRWVC 310 EKRRNATGEV A
	Isoform 2 [UniParc]. Checksum: C762F780E74A6D50	FASTA	292	32,988
	10 20 30 40 50 60 MAKDFQDIQQ LSSEENDHPF HQGPPPAQPL AQRLCSMVCF SLLALSFNIL LLVVICVTGS 70 80 90 100 110 120 QSEGHGGAQL QAELRSLKEA FSNFSSSTLT EVQAISTHGG SVGDKITSLG AKLEKQQQDL 130 140 150 160 170 180 KADHDALLFH LKHFPVDLRF VACQMELLHS NGSQRTCCPV NWVEHQGSCY WFSHSGKAWA 190 200 210 220 230 240 EAEKYCQLEN AHLVVINSWE EQKFIVQHTN PFNTWIGLTD SDGSWKWVDG TDYRHNYKNW 250 260 270 280 290 AVTQPDNWHG HELGGSEDCV EVQPDGRWND DFCLQVYRWV CEKRRNATGE VA
	Isoform 3 [UniParc]. Checksum: E9528ACF7CBB1435	FASTA	287	32,550
	10 20 30 40 50 60 MAKDFQDIQQ LSSEENDHPF HQGPPPAQPL AQRLCSMVCF SLLALSFNIL LLVVICVTGS 70 80 90 100 110 120 QSAQLQAELR SLKEAFSNFS SSTLTEVQAI STHGGSVGDK ITSLGAKLEK QQQDLKADHD 130 140 150 160 170 180 ALLFHLKHFP VDLRFVACQM ELLHSNGSQR TCCPVNWVEH QGSCYWFSHS GKAWAEAEKY 190 200 210 220 230 240 CQLENAHLVV INSWEEQKFI VQHTNPFNTW IGLTDSDGSW KWVDGTDYRH NYKNWAVTQP 250 260 270 280 DNWHGHELGG SEDCVEVQPD GRWNDDFCLQ VYRWVCEKRR NATGEVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a second human asialoglycoprotein receptor: conservation of two receptor genes during evolution." Spiess M., Lodish H.F. Proc. Natl. Acad. Sci. U.S.A. 82:6465-6469(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ARG-85.
[2]	"Differences in the abundance of variably spliced transcripts for the second asialoglycoprotein receptor polypeptide, H2, in normal and transformed human liver." Paietta E., Stockert R.J., Racevskis J. Hepatology 15:395-402(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ARG-85. Tissue: Liver.
[3]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ARG-85.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Liver.
[6]	"Cloning, mapping, and characterization of a human homologue of the yeast longevity assurance gene LAG1." Pan H., Qin W.-X., Huo K.-K., Wan D.-F., Yu Y., Xu Z.-G., Hu Q.-D., Gu K.T., Zhou X.-M., Jiang H.-Q., Zhang P.-P., Huang Y., Li Y.-Y., Gu J.-R. Genomics 77:58-64(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH LASS2.
[7]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-102, MASS SPECTROMETRY. Tissue: Plasma.
[8]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-102 AND ASN-170, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

Hepatic asialoglycoprotein receptor subunit 2

Cross-references

Sequence databases
EMBL GenBank DDBJ	M11025 mRNA. Translation: AAB59519.1. U97197 mRNA. Translation: AAB58308.1. X55283 mRNA. Translation: CAA38997.1. AC120057 Genomic DNA. No translation available. CH471108 Genomic DNA. Translation: EAW90259.1. CH471108 Genomic DNA. Translation: EAW90260.1. CH471108 Genomic DNA. Translation: EAW90261.1. CH471108 Genomic DNA. Translation: EAW90263.1. BC017251 mRNA. Translation: AAH17251.1.
IPI	IPI00011155. IPI00150200. IPI00220266.
PIR	LNHU2A. A25179.
RefSeq	NP_001172.1. NM_001181.4. NP_001188281.1. NM_001201352.1. NP_550434.1. NM_080912.3. NP_550435.1. NM_080913.3. NP_550436.1. NM_080914.2.
UniGene	Hs.654440.
3D structure databases
ProteinModelPortal	P07307.
ModBase	Search...
Protein-protein interaction databases
IntAct	P07307. 4 interactions.
STRING	9606.ENSP00000347140.
PTM databases
PhosphoSite	P07307.
Polymorphism databases
DMDM	218511923.
Proteomic databases
PaxDb	P07307.
PRIDE	P07307.
Protocols and materials databases
DNASU	433.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000254850; ENSP00000254850; ENSG00000161944. ENST00000355035; ENSP00000347140; ENSG00000161944. ENST00000380952; ENSP00000370339; ENSG00000161944. ENST00000446679; ENSP00000405844; ENSG00000161944.
GeneID	433.
KEGG	hsa:433.
UCSC	uc002gen.1. human. uc002geq.3. human.
Organism-specific databases
CTD	433.
GeneCards	GC17M007004.
HGNC	HGNC:743. ASGR2.
HPA	HPA014899. HPA015998.
MIM	108361. gene.
neXtProt	NX_P07307.
PharmGKB	PA25043.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG241599.
HOGENOM	HOG000034093.
HOVERGEN	HBG000270.
InParanoid	P07307.
KO	K10064.
OMA	QDLKADH.
OrthoDB	EOG4V171K.
Gene expression databases
ArrayExpress	P07307.
Bgee	P07307.
CleanEx	HS_ASGR2.
Genevestigator	P07307.
GermOnline	ENSG00000161944. Homo sapiens.
Family and domain databases
Gene3D	3.10.100.10. 1 hit.
InterPro	IPR001304. C-type_lectin. IPR016186. C-type_lectin-like. IPR018378. C-type_lectin_CS. IPR016187. C-type_lectin_fold. IPR005640. Lectin_N. [Graphical view]
Pfam	PF00059. Lectin_C. 1 hit. PF03954. Lectin_N. 1 hit. [Graphical view]
SMART	SM00034. CLECT. 1 hit. [Graphical view]
SUPFAM	SSF56436. C-type_lectin_fold. 1 hit.
PROSITE	PS00615. C_TYPE_LECTIN_1. 1 hit. PS50041. C_TYPE_LECTIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P07307.
ChEMBL	CHEMBL2444.
ChiTaRS	ASGR2. human.
DrugBank	DB00025. Antihemophilic Factor.
GenomeRNAi	433.
NextBio	1807.
SOURCE	Search...

Entry information

Entry name

ASGR2_HUMAN

Accession

Primary (citable) accession number: P07307
Secondary accession number(s): A6NLV8 , A8MT12, D3DTM9, D3DTN0, O00448, Q03969

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	December 16, 2008
Last modified:	May 1, 2013
This is version 136 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.