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P07306

- ASGR1_HUMAN

UniProt

P07306 - ASGR1_HUMAN

Protein

Asialoglycoprotein receptor 1

Gene

ASGR1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 150 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi191 – 1911Calcium 1; via carbonyl oxygen
    Metal bindingi197 – 1971Calcium 1
    Metal bindingi216 – 2161Calcium 2
    Metal bindingi240 – 2401Calcium 3
    Binding sitei240 – 2401Carbohydrate
    Metal bindingi242 – 2421Calcium 3
    Metal bindingi243 – 2431Calcium 2
    Binding sitei244 – 2441Carbohydrate
    Metal bindingi253 – 2531Calcium 2; via carbonyl oxygen
    Metal bindingi253 – 2531Calcium 3
    Binding sitei253 – 2531Carbohydrate
    Metal bindingi254 – 2541Calcium 2
    Metal bindingi265 – 2651Calcium 3
    Metal bindingi266 – 2661Calcium 3
    Metal bindingi278 – 2781Calcium 1

    GO - Molecular functioni

    1. asialoglycoprotein receptor activity Source: ProtInc
    2. carbohydrate binding Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: IntAct

    GO - Biological processi

    1. cellular response to extracellular stimulus Source: Ensembl
    2. receptor-mediated endocytosis Source: ProtInc

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Endocytosis

    Keywords - Ligandi

    Calcium, Lectin, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Asialoglycoprotein receptor 1
    Short name:
    ASGP-R 1
    Short name:
    ASGPR 1
    Alternative name(s):
    C-type lectin domain family 4 member H1
    Hepatic lectin H1
    Short name:
    HL-1
    Gene namesi
    Name:ASGR1
    Synonyms:CLEC4H1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:742. ASGR1.

    Subcellular locationi

    Isoform H1b : Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. integral component of plasma membrane Source: ProtInc

    Keywords - Cellular componenti

    Membrane, Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA25042.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 291291Asialoglycoprotein receptor 1PRO_0000046650Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi36 – 361S-palmitoyl cysteine1 Publication
    Glycosylationi79 – 791N-linked (GlcNAc...)1 Publication
    Glycosylationi147 – 1471N-linked (GlcNAc...)1 Publication
    Disulfide bondi154 ↔ 165
    Disulfide bondi182 ↔ 277
    Disulfide bondi255 ↔ 269

    Post-translational modificationi

    Phosphorylated on a cytoplasmic Ser residue.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    MaxQBiP07306.
    PaxDbiP07306.
    PRIDEiP07306.

    PTM databases

    PhosphoSiteiP07306.

    Expressioni

    Tissue specificityi

    Expressed exclusively in hepatic parenchymal cells.

    Gene expression databases

    ArrayExpressiP07306.
    BgeeiP07306.
    CleanExiHS_ASGR1.
    GenevestigatoriP07306.

    Organism-specific databases

    HPAiHPA011954.
    HPA012852.

    Interactioni

    Subunit structurei

    Interacts with LASS2.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CERS2Q96G233EBI-1172335,EBI-1057080

    Protein-protein interaction databases

    BioGridi106924. 12 interactions.
    IntActiP07306. 5 interactions.
    STRINGi9606.ENSP00000269299.

    Structurei

    Secondary structure

    1
    291
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi159 – 1613
    Beta strandi164 – 1685
    Helixi175 – 18410
    Helixi195 – 20511
    Beta strandi210 – 2156
    Beta strandi248 – 2514
    Beta strandi255 – 2584
    Beta strandi264 – 2674
    Beta strandi273 – 2808

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DV8X-ray2.30A154-281[»]
    ProteinModelPortaliP07306.
    SMRiP07306. Positions 154-281.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07306.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4040CytoplasmicCuratedAdd
    BLAST
    Topological domaini62 – 291230ExtracellularCuratedAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei41 – 6121Helical; Signal-anchor for type II membrane proteinCuratedAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini161 – 278118C-type lectinPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili61 – 12363Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi5 – 84Endocytosis signalSequence Analysis

    Sequence similaritiesi

    Contains 1 C-type lectin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG278574.
    HOGENOMiHOG000034093.
    HOVERGENiHBG000270.
    InParanoidiP07306.
    KOiK10063.
    OMAiQHHMGPV.
    OrthoDBiEOG7XDBGR.
    PhylomeDBiP07306.
    TreeFamiTF352155.

    Family and domain databases

    Gene3Di3.10.100.10. 1 hit.
    InterProiIPR001304. C-type_lectin.
    IPR016186. C-type_lectin-like.
    IPR018378. C-type_lectin_CS.
    IPR016187. C-type_lectin_fold.
    IPR005640. Lectin_N.
    [Graphical view]
    PfamiPF00059. Lectin_C. 1 hit.
    PF03954. Lectin_N. 1 hit.
    [Graphical view]
    SMARTiSM00034. CLECT. 1 hit.
    [Graphical view]
    SUPFAMiSSF56436. SSF56436. 1 hit.
    PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
    PS50041. C_TYPE_LECTIN_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform H1a (identifier: P07306-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTKEYQDLQH LDNEESDHHQ LRKGPPPPQP LLQRLCSGPR LLLLSLGLSL    50
    LLLVVVCVIG SQNSQLQEEL RGLRETFSNF TASTEAQVKG LSTQGGNVGR 100
    KMKSLESQLE KQQKDLSEDH SSLLLHVKQF VSDLRSLSCQ MAALQGNGSE 150
    RTCCPVNWVE HERSCYWFSR SGKAWADADN YCRLEDAHLV VVTSWEEQKF 200
    VQHHIGPVNT WMGLHDQNGP WKWVDGTDYE TGFKNWRPEQ PDDWYGHGLG 250
    GGEDCAHFTD DGRWNDDVCQ RPYRWVCETE LDKASQEPPL L 291
    Length:291
    Mass (Da):33,186
    Last modified:January 23, 2007 - v2
    Checksum:i9B825527B2FC1FD0
    GO
    Isoform H1b (identifier: P07306-2) [UniParc]FASTAAdd to Basket

    Also known as: sASGPR

    The sequence of this isoform differs from the canonical sequence as follows:
         24-63: GPPPPQPLLQRLCSGPRLLLLSLGLSLLLLVVVCVIGSQN → D

    Show »
    Length:252
    Mass (Da):29,144
    Checksum:iB0F8C6FA80E1CCF2
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei24 – 6340GPPPP…IGSQN → D in isoform H1b. 1 PublicationVSP_044480Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10058 mRNA. Translation: AAA51785.1.
    AB070933 mRNA. Translation: BAB83508.1.
    FJ410292 mRNA. Translation: ACQ90237.1.
    AC120057 Genomic DNA. No translation available.
    BC032130 mRNA. Translation: AAH32130.1.
    CCDSiCCDS11089.1. [P07306-1]
    CCDS56017.1. [P07306-2]
    PIRiA22509. LNHU1.
    RefSeqiNP_001184145.1. NM_001197216.2. [P07306-2]
    NP_001662.1. NM_001671.4. [P07306-1]
    UniGeneiHs.12056.
    Hs.731669.

    Genome annotation databases

    EnsembliENST00000269299; ENSP00000269299; ENSG00000141505. [P07306-1]
    ENST00000574388; ENSP00000459169; ENSG00000141505. [P07306-2]
    GeneIDi432.
    KEGGihsa:432.
    UCSCiuc002ges.4. human. [P07306-1]
    uc021toy.1. human. [P07306-2]

    Polymorphism databases

    DMDMi126132.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - Glycan Binding

    Hepatic asialoglycoprotein receptor subunit 1

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10058 mRNA. Translation: AAA51785.1 .
    AB070933 mRNA. Translation: BAB83508.1 .
    FJ410292 mRNA. Translation: ACQ90237.1 .
    AC120057 Genomic DNA. No translation available.
    BC032130 mRNA. Translation: AAH32130.1 .
    CCDSi CCDS11089.1. [P07306-1 ]
    CCDS56017.1. [P07306-2 ]
    PIRi A22509. LNHU1.
    RefSeqi NP_001184145.1. NM_001197216.2. [P07306-2 ]
    NP_001662.1. NM_001671.4. [P07306-1 ]
    UniGenei Hs.12056.
    Hs.731669.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DV8 X-ray 2.30 A 154-281 [» ]
    ProteinModelPortali P07306.
    SMRi P07306. Positions 154-281.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 106924. 12 interactions.
    IntActi P07306. 5 interactions.
    STRINGi 9606.ENSP00000269299.

    Chemistry

    BindingDBi P07306.
    ChEMBLi CHEMBL2084.

    PTM databases

    PhosphoSitei P07306.

    Polymorphism databases

    DMDMi 126132.

    Proteomic databases

    MaxQBi P07306.
    PaxDbi P07306.
    PRIDEi P07306.

    Protocols and materials databases

    DNASUi 432.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000269299 ; ENSP00000269299 ; ENSG00000141505 . [P07306-1 ]
    ENST00000574388 ; ENSP00000459169 ; ENSG00000141505 . [P07306-2 ]
    GeneIDi 432.
    KEGGi hsa:432.
    UCSCi uc002ges.4. human. [P07306-1 ]
    uc021toy.1. human. [P07306-2 ]

    Organism-specific databases

    CTDi 432.
    GeneCardsi GC17M007017.
    HGNCi HGNC:742. ASGR1.
    HPAi HPA011954.
    HPA012852.
    MIMi 108360. gene.
    neXtProti NX_P07306.
    PharmGKBi PA25042.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG278574.
    HOGENOMi HOG000034093.
    HOVERGENi HBG000270.
    InParanoidi P07306.
    KOi K10063.
    OMAi QHHMGPV.
    OrthoDBi EOG7XDBGR.
    PhylomeDBi P07306.
    TreeFami TF352155.

    Miscellaneous databases

    EvolutionaryTracei P07306.
    GenomeRNAii 432.
    NextBioi 1803.
    PROi P07306.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P07306.
    Bgeei P07306.
    CleanExi HS_ASGR1.
    Genevestigatori P07306.

    Family and domain databases

    Gene3Di 3.10.100.10. 1 hit.
    InterProi IPR001304. C-type_lectin.
    IPR016186. C-type_lectin-like.
    IPR018378. C-type_lectin_CS.
    IPR016187. C-type_lectin_fold.
    IPR005640. Lectin_N.
    [Graphical view ]
    Pfami PF00059. Lectin_C. 1 hit.
    PF03954. Lectin_N. 1 hit.
    [Graphical view ]
    SMARTi SM00034. CLECT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56436. SSF56436. 1 hit.
    PROSITEi PS00615. C_TYPE_LECTIN_1. 1 hit.
    PS50041. C_TYPE_LECTIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of human asialoglycoprotein receptor cDNA. An internal signal sequence for membrane insertion."
      Spiess M., Schwartz A.L., Lodish H.F.
      J. Biol. Chem. 260:1979-1982(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM H1A).
    2. "An internal signal sequence: the asialoglycoprotein receptor membrane anchor."
      Spiess M., Lodish H.F.
      Cell 44:177-185(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM H1A).
    3. "Human asialoglycoprotein receptor 1 gene is expressed in SH-SY5Y human neuroblastoma cells."
      Wang H., Gao X., Li L., Lou H., Huang Y., Wang B., Han J.
      Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM H1A).
    4. "A new splice variant of the major subunit of human asialoglycoprotein receptor encodes a secreted form in hepatocytes."
      Liu J., Hu B., Yang Y., Ma Z., Yu Y., Liu S., Wang B., Zhao X., Lu M., Yang D.
      PLoS ONE 5:E12934-E12934(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM H1B), ALTERNATIVE SPLICING, SUBCELLULAR LOCATION (ISOFORM H1B).
      Tissue: Liver.
    5. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
      Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
      , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
      Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM H1A).
      Tissue: Liver.
    7. "Fatty acylation of the rat and human asialoglycoprotein receptors. A conserved cytoplasmic cysteine residue is acylated in all receptor subunits."
      Zeng F.Y., Weigel P.H.
      J. Biol. Chem. 271:32454-32460(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PALMITOYLATION AT CYS-36.
    8. "Cloning, mapping, and characterization of a human homologue of the yeast longevity assurance gene LAG1."
      Pan H., Qin W.-X., Huo K.-K., Wan D.-F., Yu Y., Xu Z.-G., Hu Q.-D., Gu K.T., Zhou X.-M., Jiang H.-Q., Zhang P.-P., Huang Y., Li Y.-Y., Gu J.-R.
      Genomics 77:58-64(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LASS2.
    9. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
      Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
      J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-79 AND ASN-147.
      Tissue: Liver.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Crystal structure of the carbohydrate recognition domain of the H1 subunit of the asialoglycoprotein receptor."
      Meier M., Bider M.D., Malashkevich V.N., Spiess M., Burkhard P.
      J. Mol. Biol. 300:857-865(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 154-281.

    Entry informationi

    Entry nameiASGR1_HUMAN
    AccessioniPrimary (citable) accession number: P07306
    Secondary accession number(s): I3L1X1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 150 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Calcium is required for ligand binding.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3