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P07306

- ASGR1_HUMAN

UniProt

P07306 - ASGR1_HUMAN

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Protein
Asialoglycoprotein receptor 1
Gene
ASGR1, CLEC4H1
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi191 – 1911Calcium 1; via carbonyl oxygen
Metal bindingi197 – 1971Calcium 1
Metal bindingi216 – 2161Calcium 2
Metal bindingi240 – 2401Calcium 3
Binding sitei240 – 2401Carbohydrate
Metal bindingi242 – 2421Calcium 3
Metal bindingi243 – 2431Calcium 2
Binding sitei244 – 2441Carbohydrate
Metal bindingi253 – 2531Calcium 2; via carbonyl oxygen
Metal bindingi253 – 2531Calcium 3
Binding sitei253 – 2531Carbohydrate
Metal bindingi254 – 2541Calcium 2
Metal bindingi265 – 2651Calcium 3
Metal bindingi266 – 2661Calcium 3
Metal bindingi278 – 2781Calcium 1

GO - Molecular functioni

  1. asialoglycoprotein receptor activity Source: ProtInc
  2. carbohydrate binding Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. protein binding Source: IntAct

GO - Biological processi

  1. cellular response to extracellular stimulus Source: Ensembl
  2. receptor-mediated endocytosis Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Calcium, Lectin, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Asialoglycoprotein receptor 1
Short name:
ASGP-R 1
Short name:
ASGPR 1
Alternative name(s):
C-type lectin domain family 4 member H1
Hepatic lectin H1
Short name:
HL-1
Gene namesi
Name:ASGR1
Synonyms:CLEC4H1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:742. ASGR1.

Subcellular locationi

Isoform H1a : Membrane; Single-pass type II membrane protein 1 Publication
Isoform H1b : Secreted 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4040Cytoplasmic Inferred
Add
BLAST
Transmembranei41 – 6121Helical; Signal-anchor for type II membrane protein; Inferred
Add
BLAST
Topological domaini62 – 291230Extracellular Inferred
Add
BLAST

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. integral component of plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25042.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Asialoglycoprotein receptor 1
PRO_0000046650Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi36 – 361S-palmitoyl cysteine1 Publication
Glycosylationi79 – 791N-linked (GlcNAc...)1 Publication
Glycosylationi147 – 1471N-linked (GlcNAc...)1 Publication
Disulfide bondi154 ↔ 165
Disulfide bondi182 ↔ 277
Disulfide bondi255 ↔ 269

Post-translational modificationi

Phosphorylated on a cytoplasmic Ser residue.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP07306.
PaxDbiP07306.
PRIDEiP07306.

PTM databases

PhosphoSiteiP07306.

Expressioni

Tissue specificityi

Expressed exclusively in hepatic parenchymal cells.

Gene expression databases

ArrayExpressiP07306.
BgeeiP07306.
CleanExiHS_ASGR1.
GenevestigatoriP07306.

Organism-specific databases

HPAiHPA011954.
HPA012852.

Interactioni

Subunit structurei

Interacts with LASS2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CERS2Q96G233EBI-1172335,EBI-1057080

Protein-protein interaction databases

BioGridi106924. 12 interactions.
IntActiP07306. 5 interactions.
STRINGi9606.ENSP00000269299.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi159 – 1613
Beta strandi164 – 1685
Helixi175 – 18410
Helixi195 – 20511
Beta strandi210 – 2156
Beta strandi248 – 2514
Beta strandi255 – 2584
Beta strandi264 – 2674
Beta strandi273 – 2808

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DV8X-ray2.30A154-281[»]
ProteinModelPortaliP07306.
SMRiP07306. Positions 154-281.

Miscellaneous databases

EvolutionaryTraceiP07306.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini161 – 278118C-type lectin
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili61 – 12363 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi5 – 84Endocytosis signal Reviewed prediction

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG278574.
HOGENOMiHOG000034093.
HOVERGENiHBG000270.
InParanoidiP07306.
KOiK10063.
OMAiQHHMGPV.
OrthoDBiEOG7XDBGR.
PhylomeDBiP07306.
TreeFamiTF352155.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR005640. Lectin_N.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
PF03954. Lectin_N. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform H1a (identifier: P07306-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTKEYQDLQH LDNEESDHHQ LRKGPPPPQP LLQRLCSGPR LLLLSLGLSL    50
LLLVVVCVIG SQNSQLQEEL RGLRETFSNF TASTEAQVKG LSTQGGNVGR 100
KMKSLESQLE KQQKDLSEDH SSLLLHVKQF VSDLRSLSCQ MAALQGNGSE 150
RTCCPVNWVE HERSCYWFSR SGKAWADADN YCRLEDAHLV VVTSWEEQKF 200
VQHHIGPVNT WMGLHDQNGP WKWVDGTDYE TGFKNWRPEQ PDDWYGHGLG 250
GGEDCAHFTD DGRWNDDVCQ RPYRWVCETE LDKASQEPPL L 291
Length:291
Mass (Da):33,186
Last modified:January 23, 2007 - v2
Checksum:i9B825527B2FC1FD0
GO
Isoform H1b (identifier: P07306-2) [UniParc]FASTAAdd to Basket

Also known as: sASGPR

The sequence of this isoform differs from the canonical sequence as follows:
     24-63: GPPPPQPLLQRLCSGPRLLLLSLGLSLLLLVVVCVIGSQN → D

Show »
Length:252
Mass (Da):29,144
Checksum:iB0F8C6FA80E1CCF2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei24 – 6340GPPPP…IGSQN → D in isoform H1b.
VSP_044480Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10058 mRNA. Translation: AAA51785.1.
AB070933 mRNA. Translation: BAB83508.1.
FJ410292 mRNA. Translation: ACQ90237.1.
AC120057 Genomic DNA. No translation available.
BC032130 mRNA. Translation: AAH32130.1.
CCDSiCCDS11089.1. [P07306-1]
CCDS56017.1. [P07306-2]
PIRiA22509. LNHU1.
RefSeqiNP_001184145.1. NM_001197216.2. [P07306-2]
NP_001662.1. NM_001671.4. [P07306-1]
UniGeneiHs.12056.
Hs.731669.

Genome annotation databases

EnsembliENST00000269299; ENSP00000269299; ENSG00000141505. [P07306-1]
ENST00000574388; ENSP00000459169; ENSG00000141505. [P07306-2]
GeneIDi432.
KEGGihsa:432.
UCSCiuc002ges.4. human. [P07306-1]
uc021toy.1. human. [P07306-2]

Polymorphism databases

DMDMi126132.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Hepatic asialoglycoprotein receptor subunit 1

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10058 mRNA. Translation: AAA51785.1 .
AB070933 mRNA. Translation: BAB83508.1 .
FJ410292 mRNA. Translation: ACQ90237.1 .
AC120057 Genomic DNA. No translation available.
BC032130 mRNA. Translation: AAH32130.1 .
CCDSi CCDS11089.1. [P07306-1 ]
CCDS56017.1. [P07306-2 ]
PIRi A22509. LNHU1.
RefSeqi NP_001184145.1. NM_001197216.2. [P07306-2 ]
NP_001662.1. NM_001671.4. [P07306-1 ]
UniGenei Hs.12056.
Hs.731669.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DV8 X-ray 2.30 A 154-281 [» ]
ProteinModelPortali P07306.
SMRi P07306. Positions 154-281.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 106924. 12 interactions.
IntActi P07306. 5 interactions.
STRINGi 9606.ENSP00000269299.

Chemistry

BindingDBi P07306.
ChEMBLi CHEMBL2084.

PTM databases

PhosphoSitei P07306.

Polymorphism databases

DMDMi 126132.

Proteomic databases

MaxQBi P07306.
PaxDbi P07306.
PRIDEi P07306.

Protocols and materials databases

DNASUi 432.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000269299 ; ENSP00000269299 ; ENSG00000141505 . [P07306-1 ]
ENST00000574388 ; ENSP00000459169 ; ENSG00000141505 . [P07306-2 ]
GeneIDi 432.
KEGGi hsa:432.
UCSCi uc002ges.4. human. [P07306-1 ]
uc021toy.1. human. [P07306-2 ]

Organism-specific databases

CTDi 432.
GeneCardsi GC17M007017.
HGNCi HGNC:742. ASGR1.
HPAi HPA011954.
HPA012852.
MIMi 108360. gene.
neXtProti NX_P07306.
PharmGKBi PA25042.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG278574.
HOGENOMi HOG000034093.
HOVERGENi HBG000270.
InParanoidi P07306.
KOi K10063.
OMAi QHHMGPV.
OrthoDBi EOG7XDBGR.
PhylomeDBi P07306.
TreeFami TF352155.

Miscellaneous databases

EvolutionaryTracei P07306.
GenomeRNAii 432.
NextBioi 1803.
PROi P07306.
SOURCEi Search...

Gene expression databases

ArrayExpressi P07306.
Bgeei P07306.
CleanExi HS_ASGR1.
Genevestigatori P07306.

Family and domain databases

Gene3Di 3.10.100.10. 1 hit.
InterProi IPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR005640. Lectin_N.
[Graphical view ]
Pfami PF00059. Lectin_C. 1 hit.
PF03954. Lectin_N. 1 hit.
[Graphical view ]
SMARTi SM00034. CLECT. 1 hit.
[Graphical view ]
SUPFAMi SSF56436. SSF56436. 1 hit.
PROSITEi PS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of human asialoglycoprotein receptor cDNA. An internal signal sequence for membrane insertion."
    Spiess M., Schwartz A.L., Lodish H.F.
    J. Biol. Chem. 260:1979-1982(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM H1A).
  2. "An internal signal sequence: the asialoglycoprotein receptor membrane anchor."
    Spiess M., Lodish H.F.
    Cell 44:177-185(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM H1A).
  3. "Human asialoglycoprotein receptor 1 gene is expressed in SH-SY5Y human neuroblastoma cells."
    Wang H., Gao X., Li L., Lou H., Huang Y., Wang B., Han J.
    Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM H1A).
  4. "A new splice variant of the major subunit of human asialoglycoprotein receptor encodes a secreted form in hepatocytes."
    Liu J., Hu B., Yang Y., Ma Z., Yu Y., Liu S., Wang B., Zhao X., Lu M., Yang D.
    PLoS ONE 5:E12934-E12934(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM H1B), ALTERNATIVE SPLICING, SUBCELLULAR LOCATION (ISOFORM H1B).
    Tissue: Liver.
  5. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM H1A).
    Tissue: Liver.
  7. "Fatty acylation of the rat and human asialoglycoprotein receptors. A conserved cytoplasmic cysteine residue is acylated in all receptor subunits."
    Zeng F.Y., Weigel P.H.
    J. Biol. Chem. 271:32454-32460(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-36.
  8. "Cloning, mapping, and characterization of a human homologue of the yeast longevity assurance gene LAG1."
    Pan H., Qin W.-X., Huo K.-K., Wan D.-F., Yu Y., Xu Z.-G., Hu Q.-D., Gu K.T., Zhou X.-M., Jiang H.-Q., Zhang P.-P., Huang Y., Li Y.-Y., Gu J.-R.
    Genomics 77:58-64(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LASS2.
  9. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-79 AND ASN-147.
    Tissue: Liver.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Crystal structure of the carbohydrate recognition domain of the H1 subunit of the asialoglycoprotein receptor."
    Meier M., Bider M.D., Malashkevich V.N., Spiess M., Burkhard P.
    J. Mol. Biol. 300:857-865(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 154-281.

Entry informationi

Entry nameiASGR1_HUMAN
AccessioniPrimary (citable) accession number: P07306
Secondary accession number(s): I3L1X1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Calcium is required for ligand binding.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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