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P07306 (ASGR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 146. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Asialoglycoprotein receptor 1

Short name=ASGP-R 1
Short name=ASGPR 1
Alternative name(s):
C-type lectin domain family 4 member H1
Hepatic lectin H1
Short name=HL-1
Gene names
Name:ASGR1
Synonyms:CLEC4H1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

Subunit structure

Interacts with LASS2. Ref.8

Subcellular location

Isoform H1a: Membrane; Single-pass type II membrane protein Ref.4.

Isoform H1b: Secreted Ref.4.

Tissue specificity

Expressed exclusively in hepatic parenchymal cells.

Post-translational modification

Phosphorylated on a cytoplasmic Ser residue.

Miscellaneous

Calcium is required for ligand binding.

Sequence similarities

Contains 1 C-type lectin domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CERS2Q96G233EBI-1172335,EBI-1057080

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform H1a (identifier: P07306-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform H1b (identifier: P07306-2)

Also known as: sASGPR;

The sequence of this isoform differs from the canonical sequence as follows:
     24-63: GPPPPQPLLQRLCSGPRLLLLSLGLSLLLLVVVCVIGSQN → D

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291Asialoglycoprotein receptor 1
PRO_0000046650

Regions

Topological domain1 – 4040Cytoplasmic Probable
Transmembrane41 – 6121Helical; Signal-anchor for type II membrane protein; Probable
Topological domain62 – 291230Extracellular Probable
Domain161 – 278118C-type lectin
Coiled coil61 – 12363 Potential
Motif5 – 84Endocytosis signal Potential

Sites

Metal binding1911Calcium 1; via carbonyl oxygen
Metal binding1971Calcium 1
Metal binding2161Calcium 2
Metal binding2401Calcium 3
Metal binding2421Calcium 3
Metal binding2431Calcium 2
Metal binding2531Calcium 2; via carbonyl oxygen
Metal binding2531Calcium 3
Metal binding2541Calcium 2
Metal binding2651Calcium 3
Metal binding2661Calcium 3
Metal binding2781Calcium 1
Binding site2401Carbohydrate
Binding site2441Carbohydrate
Binding site2531Carbohydrate

Amino acid modifications

Lipidation361S-palmitoyl cysteine Ref.7
Glycosylation791N-linked (GlcNAc...) Ref.9
Glycosylation1471N-linked (GlcNAc...) Ref.9
Disulfide bond154 ↔ 165
Disulfide bond182 ↔ 277
Disulfide bond255 ↔ 269

Natural variations

Alternative sequence24 – 6340GPPPP…IGSQN → D in isoform H1b.
VSP_044480

Secondary structure

................... 291
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform H1a [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 9B825527B2FC1FD0

FASTA29133,186
        10         20         30         40         50         60 
MTKEYQDLQH LDNEESDHHQ LRKGPPPPQP LLQRLCSGPR LLLLSLGLSL LLLVVVCVIG 

        70         80         90        100        110        120 
SQNSQLQEEL RGLRETFSNF TASTEAQVKG LSTQGGNVGR KMKSLESQLE KQQKDLSEDH 

       130        140        150        160        170        180 
SSLLLHVKQF VSDLRSLSCQ MAALQGNGSE RTCCPVNWVE HERSCYWFSR SGKAWADADN 

       190        200        210        220        230        240 
YCRLEDAHLV VVTSWEEQKF VQHHIGPVNT WMGLHDQNGP WKWVDGTDYE TGFKNWRPEQ 

       250        260        270        280        290 
PDDWYGHGLG GGEDCAHFTD DGRWNDDVCQ RPYRWVCETE LDKASQEPPL L 

« Hide

Isoform H1b (sASGPR) [UniParc].

Checksum: B0F8C6FA80E1CCF2
Show »

FASTA25229,144

References

« Hide 'large scale' references
[1]"Sequence of human asialoglycoprotein receptor cDNA. An internal signal sequence for membrane insertion."
Spiess M., Schwartz A.L., Lodish H.F.
J. Biol. Chem. 260:1979-1982(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM H1A).
[2]"An internal signal sequence: the asialoglycoprotein receptor membrane anchor."
Spiess M., Lodish H.F.
Cell 44:177-185(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM H1A).
[3]"Human asialoglycoprotein receptor 1 gene is expressed in SH-SY5Y human neuroblastoma cells."
Wang H., Gao X., Li L., Lou H., Huang Y., Wang B., Han J.
Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM H1A).
[4]"A new splice variant of the major subunit of human asialoglycoprotein receptor encodes a secreted form in hepatocytes."
Liu J., Hu B., Yang Y., Ma Z., Yu Y., Liu S., Wang B., Zhao X., Lu M., Yang D.
PLoS ONE 5:E12934-E12934(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM H1B), ALTERNATIVE SPLICING, SUBCELLULAR LOCATION (ISOFORM H1B).
Tissue: Liver.
[5]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM H1A).
Tissue: Liver.
[7]"Fatty acylation of the rat and human asialoglycoprotein receptors. A conserved cytoplasmic cysteine residue is acylated in all receptor subunits."
Zeng F.Y., Weigel P.H.
J. Biol. Chem. 271:32454-32460(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PALMITOYLATION AT CYS-36.
[8]"Cloning, mapping, and characterization of a human homologue of the yeast longevity assurance gene LAG1."
Pan H., Qin W.-X., Huo K.-K., Wan D.-F., Yu Y., Xu Z.-G., Hu Q.-D., Gu K.T., Zhou X.-M., Jiang H.-Q., Zhang P.-P., Huang Y., Li Y.-Y., Gu J.-R.
Genomics 77:58-64(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LASS2.
[9]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-79 AND ASN-147.
Tissue: Liver.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Crystal structure of the carbohydrate recognition domain of the H1 subunit of the asialoglycoprotein receptor."
Meier M., Bider M.D., Malashkevich V.N., Spiess M., Burkhard P.
J. Mol. Biol. 300:857-865(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 154-281.
+Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

Hepatic asialoglycoprotein receptor subunit 1

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M10058 mRNA. Translation: AAA51785.1.
AB070933 mRNA. Translation: BAB83508.1.
FJ410292 mRNA. Translation: ACQ90237.1.
AC120057 Genomic DNA. No translation available.
BC032130 mRNA. Translation: AAH32130.1.
PIRLNHU1. A22509.
RefSeqNP_001184145.1. NM_001197216.2.
NP_001662.1. NM_001671.4.
UniGeneHs.12056.
Hs.731669.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1DV8X-ray2.30A154-281[»]
ProteinModelPortalP07306.
SMRP07306. Positions 154-281.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid106924. 12 interactions.
IntActP07306. 5 interactions.
STRING9606.ENSP00000269299.

Chemistry

BindingDBP07306.
ChEMBLCHEMBL2084.

PTM databases

PhosphoSiteP07306.

Polymorphism databases

DMDM126132.

Proteomic databases

PaxDbP07306.
PRIDEP07306.

Protocols and materials databases

DNASU432.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000269299; ENSP00000269299; ENSG00000141505. [P07306-1]
ENST00000574388; ENSP00000459169; ENSG00000141505. [P07306-2]
GeneID432.
KEGGhsa:432.
UCSCuc002ges.4. human. [P07306-1]
uc021toy.1. human. [P07306-2]

Organism-specific databases

CTD432.
GeneCardsGC17M007017.
HGNCHGNC:742. ASGR1.
HPAHPA011954.
HPA012852.
MIM108360. gene.
neXtProtNX_P07306.
PharmGKBPA25042.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG278574.
HOGENOMHOG000034093.
HOVERGENHBG000270.
InParanoidP07306.
KOK10063.
OMAQHHMGPV.
OrthoDBEOG7XDBGR.
PhylomeDBP07306.
TreeFamTF352155.

Gene expression databases

ArrayExpressP07306.
BgeeP07306.
CleanExHS_ASGR1.
GenevestigatorP07306.

Family and domain databases

Gene3D3.10.100.10. 1 hit.
InterProIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR005640. Lectin_N.
[Graphical view]
PfamPF00059. Lectin_C. 1 hit.
PF03954. Lectin_N. 1 hit.
[Graphical view]
SMARTSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMSSF56436. SSF56436. 1 hit.
PROSITEPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP07306.
GenomeRNAi432.
NextBio1803.
PROP07306.
SOURCESearch...

Entry information

Entry nameASGR1_HUMAN
AccessionPrimary (citable) accession number: P07306
Secondary accession number(s): I3L1X1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM