P07300 (VNCS_MUMIM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-capsid protein NS-1

Alternative name(s):
NCVP1
Non-structural protein NS1

Gene names

Name:

NS1

Organism

Murine minute virus (strain MVMi) (MVM) (Murine parvovirus) [Complete proteome]

Taxonomic identifier

10795 [NCBI]

Taxonomic lineage

Viruses › ssDNA viruses › Parvoviridae › Parvovirinae › Parvovirus

Virus host

Mus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length	672 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Seems necessary for viral DNA replication.
Subunit structure	Forms homooligomers. Interacts with human SYNCRIP By similarity.
Domain	The N-terminus (residues 1-275) possess a negative effect on transactivation By similarity. The C-terminus (residues 543-672) possess an activation domain By similarity.
Sequence similarities	Belongs to the parvoviruses non-capsid protein family. Contains 1 SF3 helicase domain.

Ontologies

Keywords
Biological process	DNA replication Host-virus interaction Initiation of viral infection Viral penetration into host cytoplasm Viral penetration via permeabilization of host organellar membrane
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 672	672	Non-capsid protein NS-1		PRO_0000222465
Regions
	Domain	373 – 528	156	SF3 helicase
	Nucleotide binding	399 – 406	8	ATP Potential
	Region	1 – 276	276	Interacts with SYNCRIP By similarity
Experimental info
	Sequence conflict	597	1	I → L Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P07300 [UniParc]. Last modified November 1, 1991. Version 2. Checksum: 25F025FE328B4DF0	FASTA	672	76,140
	10 20 30 40 50 60 MAGNAYSDEV LGTTNWLKEK SNQEVFSFVF KTEDVQLNGK DIGWNNYKKE LQEDELKSLQ 70 80 90 100 110 120 RGAETTWDQS EDMEWESTVD EMTKKQVFIY DSLVKKCLFE VLSTKNIAPA DVTWFVQHEW 130 140 150 160 170 180 GKDQGWHCHV LIGGKDFSQA QGKWWRRQLN VYWSRWLVTA CNVQLTPAER IKLREIAEDS 190 200 210 220 230 240 EWVTLLTYKH KQTKKDYTKC VLFGNMIAYY FLTKKKISTS PPRDGGYFLS SDSGWKTNFL 250 260 270 280 290 300 KEGERHLVSK LYTDDMRPET VETTVTTAQE TKRGRIQTKK EVSIKTTLKE LVHKRVTSPE 310 320 330 340 350 360 DWMMMQPDSY IEMMAQPGGE NLLKNTLEIC TLTLARTKTA FDLILEKAET SKLTNFSLPD 370 380 390 400 410 420 TRTCKIFAFH GWNYVKVCHA ICCVLNRQGG KRNTVLFHGP ASTGKSIIAQ AIAQAVGNVG 430 440 450 460 470 480 CYNAANVNFP FNDCTNKNLI WVEEAGNFGQ QVNQFKAICS GQTIRIDQKG KGSKQIEPTP 490 500 510 520 530 540 VIMTTNENIT VVRIGCEERP EHTQPIRDRM LNIHLTHTLP GDFGLVDKNE WPMICAWLVK 550 560 570 580 590 600 NGYQSTMASY CAKWGKVPDW SENWAEPKVP TPINSLGSAR SPFTTPKSTP LSQNYAITPL 610 620 630 640 650 660 ASDLEDLALE PWSTPNTPVA GTAETQNTGE AGSKACQDGQ LSPTWSEIEE DLRACFGAEP 670 LKRDFSEPLN LD

References

[1]	"DNA sequence of the lymphotropic variant of minute virus of mice, MVM(i), and comparison with the DNA sequence of the fibrotropic prototype strain." Astell C.R., Gardiner E.M., Tattersall P. J. Virol. 57:656-669(1986) [PubMed: 3502703] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"DNA sequence comparison between two tissue-specific variants of the autonomous parvovirus, minute virus of mice." Sahli R., McMaster G.K., Hirt B. Nucleic Acids Res. 13:3617-3633(1985) [PubMed: 3855242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02481 Genomic DNA. No translation available. M12032 Genomic DNA. Translation: AAA69567.1.
3D structure databases
ProteinModelPortal	P07300.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR014015. Helicase_SF3_DNA-vir. IPR021972. Parvovirus_NS1_C. IPR001257. Parvovirus_NS1_helicase. IPR021076. Parvovirus_NS1_N. [Graphical view]
Pfam	PF12117. DUF3580. 1 hit. PF01057. Parvo_NS1. 1 hit. PF12433. PV_NSP1. 1 hit. [Graphical view]
PROSITE	PS51206. SF3_HELICASE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VNCS_MUMIM

Accession

Primary (citable) accession number: P07300
Secondary accession number(s): P10837

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	November 1, 1991
Last modified:	September 21, 2011
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program