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Protein

Voltage-dependent L-type calcium channel subunit alpha-1S

Gene

CACNA1S

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1S gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1S subunit play an important role in excitation-contraction coupling in skeletal muscle.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei292 – 2921Calcium ion selectivity and permeabilityBy similarity
Sitei614 – 6141Calcium ion selectivity and permeabilityBy similarity
Sitei1014 – 10141Calcium ion selectivity and permeabilityBy similarity
Sitei1323 – 13231Calcium ion selectivity and permeabilityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi1410 – 142112By similarityAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

TCDBi1.A.1.11.2. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent L-type calcium channel subunit alpha-1S
Alternative name(s):
Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle
Voltage-gated calcium channel subunit alpha Cav1.1
Gene namesi
Name:CACNA1S
Synonyms:CACH1, CACNL1A3
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5151CytoplasmicSequence analysisAdd
BLAST
Transmembranei52 – 7019Helical; Name=S1 of repeat ISequence analysisAdd
BLAST
Topological domaini71 – 8818ExtracellularSequence analysisAdd
BLAST
Transmembranei89 – 10820Helical; Name=S2 of repeat ISequence analysisAdd
BLAST
Topological domaini109 – 12012CytoplasmicSequence analysisAdd
BLAST
Transmembranei121 – 13919Helical; Name=S3 of repeat ISequence analysisAdd
BLAST
Topological domaini140 – 16021ExtracellularSequence analysisAdd
BLAST
Transmembranei161 – 17919Helical; Name=S4 of repeat ISequence analysisAdd
BLAST
Topological domaini180 – 19819CytoplasmicSequence analysisAdd
BLAST
Transmembranei199 – 21820Helical; Name=S5 of repeat ISequence analysisAdd
BLAST
Topological domaini219 – 30991ExtracellularSequence analysisAdd
BLAST
Transmembranei310 – 33425Helical; Name=S6 of repeat ISequence analysisAdd
BLAST
Topological domaini335 – 43298CytoplasmicSequence analysisAdd
BLAST
Transmembranei433 – 45119Helical; Name=S1 of repeat IISequence analysisAdd
BLAST
Topological domaini452 – 46615ExtracellularSequence analysisAdd
BLAST
Transmembranei467 – 48620Helical; Name=S2 of repeat IISequence analysisAdd
BLAST
Topological domaini487 – 4948CytoplasmicSequence analysis
Transmembranei495 – 51319Helical; Name=S3 of repeat IISequence analysisAdd
BLAST
Topological domaini514 – 52310ExtracellularSequence analysis
Transmembranei524 – 54219Helical; Name=S4 of repeat IISequence analysisAdd
BLAST
Topological domaini543 – 56119CytoplasmicSequence analysisAdd
BLAST
Transmembranei562 – 58120Helical; Name=S5 of repeat IISequence analysisAdd
BLAST
Topological domaini582 – 63655ExtracellularSequence analysisAdd
BLAST
Transmembranei637 – 66125Helical; Name=S6 of repeat IISequence analysisAdd
BLAST
Topological domaini662 – 799138CytoplasmicSequence analysisAdd
BLAST
Transmembranei800 – 81819Helical; Name=S1 of repeat IIISequence analysisAdd
BLAST
Topological domaini819 – 83416ExtracellularSequence analysisAdd
BLAST
Transmembranei835 – 85420Helical; Name=S2 of repeat IIISequence analysisAdd
BLAST
Topological domaini855 – 86612CytoplasmicSequence analysisAdd
BLAST
Transmembranei867 – 88519Helical; Name=S3 of repeat IIISequence analysisAdd
BLAST
Topological domaini886 – 8927ExtracellularSequence analysis
Transmembranei893 – 91119Helical; Name=S4 of repeat IIISequence analysisAdd
BLAST
Topological domaini912 – 93019CytoplasmicSequence analysisAdd
BLAST
Transmembranei931 – 95020Helical; Name=S5 of repeat IIISequence analysisAdd
BLAST
Topological domaini951 – 104090ExtracellularSequence analysisAdd
BLAST
Transmembranei1041 – 106525Helical; Name=S6 of repeat IIISequence analysisAdd
BLAST
Topological domaini1066 – 111853CytoplasmicSequence analysisAdd
BLAST
Transmembranei1119 – 113719Helical; Name=S1 of repeat IVSequence analysisAdd
BLAST
Topological domaini1138 – 115215ExtracellularSequence analysisAdd
BLAST
Transmembranei1153 – 117220Helical; Name=S2 of repeat IVSequence analysisAdd
BLAST
Topological domaini1173 – 11808CytoplasmicSequence analysis
Transmembranei1181 – 119919Helical; Name=S3 of repeat IVSequence analysisAdd
BLAST
Topological domaini1200 – 123132ExtracellularSequence analysisAdd
BLAST
Transmembranei1232 – 125019Helical; Name=S4 of repeat IVSequence analysisAdd
BLAST
Topological domaini1251 – 126919CytoplasmicSequence analysisAdd
BLAST
Transmembranei1270 – 128920Helical; Name=S5 of repeat IVSequence analysisAdd
BLAST
Topological domaini1290 – 135667ExtracellularSequence analysisAdd
BLAST
Transmembranei1357 – 138125Helical; Name=S6 of repeat IVSequence analysisAdd
BLAST
Topological domaini1382 – 1873492CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4169.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18731873Voltage-dependent L-type calcium channel subunit alpha-1SPRO_0000053945Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence analysis
Glycosylationi257 – 2571N-linked (GlcNAc...)Sequence analysis
Modified residuei393 – 3931PhosphoserineBy similarity
Modified residuei397 – 3971PhosphoserineBy similarity
Modified residuei687 – 6871Phosphoserine; by PKA1 Publication
Modified residuei1392 – 13921Phosphoserine; by PKASequence analysis
Modified residuei1575 – 15751PhosphoserineBy similarity
Modified residuei1579 – 15791PhosphothreonineBy similarity
Modified residuei1617 – 16171Phosphoserine; by PKA1 Publication

Post-translational modificationi

The alpha-1S subunit is found in two isoforms in the skeletal muscle: a minor form of 212 kDa containing the complete amino acid sequence, and a major form of 190 kDa derived from the full-length form by post-translational proteolysis close to Phe-1690.
Both the minor and major forms are phosphorylated in vitro by PKA. Phosphorylation by PKA activates the calcium channel.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei1690 – 16912CleavageCurated

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

PTM databases

iPTMnetiP07293.
SwissPalmiP07293.

Expressioni

Tissue specificityi

Skeletal muscle specific.

Interactioni

Subunit structurei

Multisubunit complex consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. An additional gamma subunit is present only in skeletal muscle L-type channel. Interacts with DYSF and JSRP1 (By similarity). Interacts with RYR1.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PASKQ96RG22EBI-8613624,EBI-1042651From a different organism.

Protein-protein interaction databases

DIPiDIP-61879N.
IntActiP07293. 4 interactions.
MINTiMINT-8146707.

Structurei

Secondary structure

1
1873
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi359 – 37214Combined sources
Helixi672 – 6776Combined sources
Turni678 – 6814Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DU1NMR-A671-690[»]
1JZPNMR-A671-690[»]
1T3LX-ray2.20B357-374[»]
3JBRelectron microscopy4.20A1-1395[»]
A1521-1873[»]
DisProtiDP00228.
ProteinModelPortaliP07293.
SMRiP07293. Positions 1517-1543.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07293.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati38 – 337300IAdd
BLAST
Repeati418 – 664247IIAdd
BLAST
Repeati786 – 1068283IIIAdd
BLAST
Repeati1105 – 1384280IVAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni357 – 37418Binding to the beta subunitAdd
BLAST
Regioni988 – 107790Dihydropyridine bindingAdd
BLAST
Regioni1337 – 140367Dihydropyridine bindingAdd
BLAST
Regioni1349 – 139143Phenylalkylamine bindingAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi562 – 5687Poly-Leu

Domaini

Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
The loop between repeats II and III interacts with the ryanodine receptor, and is therefore important for calcium release from the endoplasmic reticulum necessary for muscle contraction.

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000231529.
HOVERGENiHBG050763.
InParanoidiP07293.
KOiK04857.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR031688. CAC1F_C.
IPR027359. Channel_four-helix_dom.
IPR031649. GPHH_dom.
IPR005821. Ion_trans_dom.
IPR014873. VDCC_a1su_IQ.
IPR005450. VDCC_L_a1ssu.
IPR005446. VDCC_L_a1su.
IPR002077. VDCCAlpha1.
[Graphical view]
PANTHERiPTHR10037:SF190. PTHR10037:SF190. 2 hits.
PfamiPF08763. Ca_chan_IQ. 1 hit.
PF16885. CAC1F_C. 1 hit.
PF16905. GPHH. 1 hit.
PF00520. Ion_trans. 4 hits.
[Graphical view]
PRINTSiPR00167. CACHANNEL.
PR01630. LVDCCALPHA1.
PR01634. LVDCCALPHA1S.
SMARTiSM01062. Ca_chan_IQ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07293-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPSSPQDEG LRKKQPKKPL PEVLPRPPRA LFCLTLQNPL RKACISIVEW
60 70 80 90 100
KPFETIILLT IFANCVALAV YLPMPEDDNN SLNLGLEKLE YFFLTVFSIE
110 120 130 140 150
AAMKIIAYGF LFHQDAYLRS GWNVLDFIIV FLGVFTAILE QVNVIQSNTA
160 170 180 190 200
PMSSKGAGLD VKALRAFRVL RPLRLVSGVP SLQVVLNSIF KAMLPLFHIA
210 220 230 240 250
LLVLFMVIIY AIIGLELFKG KMHKTCYYIG TDIVATVENE KPSPCARTGS
260 270 280 290 300
GRPCTINGSE CRGGWPGPNH GITHFDNFGF SMLTVYQCIT MEGWTDVLYW
310 320 330 340 350
VNDAIGNEWP WIYFVTLILL GSFFILNLVL GVLSGEFTKE REKAKSRGTF
360 370 380 390 400
QKLREKQQLE EDLRGYMSWI TQGEVMDVED LREGKLSLEE GGSDTESLYE
410 420 430 440 450
IEGLNKIIQF IRHWRQWNRV FRWKCHDLVK SRVFYWLVIL IVALNTLSIA
460 470 480 490 500
SEHHNQPLWL THLQDIANRV LLSLFTIEML LKMYGLGLRQ YFMSIFNRFD
510 520 530 540 550
CFVVCSGILE LLLVESGAMT PLGISVLRCI RLLRLFKITK YWTSLSNLVA
560 570 580 590 600
SLLNSIRSIA SLLLLLFLFI IIFALLGMQL FGGRYDFEDT EVRRSNFDNF
610 620 630 640 650
PQALISVFQV LTGEDWNSVM YNGIMAYGGP SYPGVLVCIY FIILFVCGNY
660 670 680 690 700
ILLNVFLAIA VDNLAEAESL TSAQKAKAEE RKRRKMSRGL PDKTEEEKSV
710 720 730 740 750
MAKKLEQKPK GEGIPTTAKL KVDEFESNVN EVKDPYPSAD FPGDDEEDEP
760 770 780 790 800
EIPVSPRPRP LAELQLKEKA VPIPEASSFF IFSPTNKVRV LCHRIVNATW
810 820 830 840 850
FTNFILLFIL LSSAALAAED PIRAESVRNQ ILGYFDIAFT SVFTVEIVLK
860 870 880 890 900
MTTYGAFLHK GSFCRNYFNI LDLLVVAVSL ISMGLESSTI SVVKILRVLR
910 920 930 940 950
VLRPLRAINR AKGLKHVVQC VFVAIRTIGN IVLVTTLLQF MFACIGVQLF
960 970 980 990 1000
KGKFFSCNDL SKMTEEECRG YYYVYKDGDP TQMELRPRQW IHNDFHFDNV
1010 1020 1030 1040 1050
LSAMMSLFTV STFEGWPQLL YRAIDSNEED MGPVYNNRVE MAIFFIIYII
1060 1070 1080 1090 1100
LIAFFMMNIF VGFVIVTFQE QGETEYKNCE LDKNQRQCVQ YALKARPLRC
1110 1120 1130 1140 1150
YIPKNPYQYQ VWYVVTSSYF EYLMFALIML NTICLGMQHY HQSEEMNHIS
1160 1170 1180 1190 1200
DILNVAFTII FTLEMILKLL AFKARGYFGD PWNVFDFLIV IGSIIDVILS
1210 1220 1230 1240 1250
EIDTFLASSG GLYCLGGGCG NVDPDESARI SSAFFRLFRV MRLIKLLSRA
1260 1270 1280 1290 1300
EGVRTLLWTF IKSFQALPYV ALLIVMLFFI YAVIGMQMFG KIALVDGTQI
1310 1320 1330 1340 1350
NRNNNFQTFP QAVLLLFRCA TGEAWQEILL ACSYGKLCDP ESDYAPGEEY
1360 1370 1380 1390 1400
TCGTNFAYYY FISFYMLCAF LIINLFVAVI MDNFDYLTRD WSILGPHHLD
1410 1420 1430 1440 1450
EFKAIWAEYD PEAKGRIKHL DVVTLLRRIQ PPLGFGKFCP HRVACKRLVG
1460 1470 1480 1490 1500
MNMPLNSDGT VTFNATLFAL VRTALKIKTE GNFEQANEEL RAIIKKIWKR
1510 1520 1530 1540 1550
TSMKLLDQVI PPIGDDEVTV GKFYATFLIQ EHFRKFMKRQ EEYYGYRPKK
1560 1570 1580 1590 1600
DTVQIQAGLR TIEEEAAPEI RRTISGDLTA EEELERAMVE AAMEERIFRR
1610 1620 1630 1640 1650
TGGLFGQVDT FLERTNSLPP VMANQRPLQF AEIEMEELES PVFLEDFPQD
1660 1670 1680 1690 1700
ARTNPLARAN TNNANANVAY GNSNHSNNQM FSSVHCEREF PGEAETPAAG
1710 1720 1730 1740 1750
RGALSHSHRA LGPHSKPCAG KLNGQLVQPG MPINQAPPAP CQQPSTDPPE
1760 1770 1780 1790 1800
RGQRRTSLTG SLQDEAPQRR SSEGSTPRRP APATALLIQE ALVRGGLDTL
1810 1820 1830 1840 1850
AADAGFVTAT SQALADACQM EPEEVEVAAT ELLKARESVQ GMASVPGSLS
1860 1870
RRSSLGSLDQ VQGSQETLIP PRP
Length:1,873
Mass (Da):212,029
Last modified:April 1, 1988 - v1
Checksum:i047B10D1946B0796
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti694 – 6941T → R in AAA31159 (PubMed:2458626).Curated
Sequence conflicti1808 – 18081T → M in AAA31159 (PubMed:2458626).Curated
Sequence conflicti1815 – 18151A → V in AAA31159 (PubMed:2458626).Curated
Sequence conflicti1835 – 18351A → E in AAA31159 (PubMed:2458626).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti165 – 1651R → K.
Natural varianti258 – 2581G → D.
Natural varianti1870 – 18701P → L.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05921 mRNA. Translation: CAA29355.1.
M23919 mRNA. Translation: AAA31159.1.
PIRiA30063.
RefSeqiNP_001095190.1. NM_001101720.1.
UniGeneiOcu.1826.

Genome annotation databases

GeneIDi100009585.
KEGGiocu:100009585.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05921 mRNA. Translation: CAA29355.1.
M23919 mRNA. Translation: AAA31159.1.
PIRiA30063.
RefSeqiNP_001095190.1. NM_001101720.1.
UniGeneiOcu.1826.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DU1NMR-A671-690[»]
1JZPNMR-A671-690[»]
1T3LX-ray2.20B357-374[»]
3JBRelectron microscopy4.20A1-1395[»]
A1521-1873[»]
DisProtiDP00228.
ProteinModelPortaliP07293.
SMRiP07293. Positions 1517-1543.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61879N.
IntActiP07293. 4 interactions.
MINTiMINT-8146707.

Chemistry

ChEMBLiCHEMBL4169.

Protein family/group databases

TCDBi1.A.1.11.2. the voltage-gated ion channel (vic) superfamily.

PTM databases

iPTMnetiP07293.
SwissPalmiP07293.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100009585.
KEGGiocu:100009585.

Organism-specific databases

CTDi779.

Phylogenomic databases

HOGENOMiHOG000231529.
HOVERGENiHBG050763.
InParanoidiP07293.
KOiK04857.

Miscellaneous databases

EvolutionaryTraceiP07293.
PROiP07293.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR031688. CAC1F_C.
IPR027359. Channel_four-helix_dom.
IPR031649. GPHH_dom.
IPR005821. Ion_trans_dom.
IPR014873. VDCC_a1su_IQ.
IPR005450. VDCC_L_a1ssu.
IPR005446. VDCC_L_a1su.
IPR002077. VDCCAlpha1.
[Graphical view]
PANTHERiPTHR10037:SF190. PTHR10037:SF190. 2 hits.
PfamiPF08763. Ca_chan_IQ. 1 hit.
PF16885. CAC1F_C. 1 hit.
PF16905. GPHH. 1 hit.
PF00520. Ion_trans. 4 hits.
[Graphical view]
PRINTSiPR00167. CACHANNEL.
PR01630. LVDCCALPHA1.
PR01634. LVDCCALPHA1S.
SMARTiSM01062. Ca_chan_IQ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAC1S_RABIT
AccessioniPrimary (citable) accession number: P07293
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: July 6, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.