P07291 (MLE_AEQIR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Myosin essential light chain, striated adductor muscle Short name=E-LC Alternative name(s): Sulfhydryl light chain Short name=SHLC
Organism	Aequipecten irradians (Bay scallop) (Argopecten irradians)
Taxonomic identifier	31199 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Mollusca › Bivalvia › Pteriomorphia › Pectinoida › Pectinoidea › Pectinidae › Argopecten

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In molluscan muscle, calcium regulation is associated with myosin rather than with actin. Muscle myosin contains two types of light chains: the catalytic light chain, essential for ATPase activity, and the regulatory light chain, a calcium-binding protein responsible for Ca2+ dependent binding and Ca2+ dependent Mg-ATPase activity.
Sequence similarities	Contains 2 EF-hand domains.

Ontologies

Keywords
Domain	Repeat
Molecular function	Motor protein Muscle protein Myosin
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular component	myosin complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2

Chain

2 – 157

156

Myosin essential light chain, striated adductor muscle

PRO_0000198718

Regions

Domain

7 – 44

38

EF-hand 1

Domain

82 – 117

36

EF-hand 2

Secondary structure

1

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157

Helix Strand Turn

Details...

Helix

6 – 22

17

Beta strand

25 – 27

3

Helix

31 – 33

3

Helix

34 – 40

7

Helix

47 – 52

6

Beta strand

57 – 61

5

Helix

66 – 76

11

Helix

84 – 92

9

Beta strand

99 – 103

5

Helix

104 – 113

10

Beta strand

114 – 116

3

Helix

120 – 130

11

Beta strand

138 – 141

4

Helix

142 – 151

10

Sequences

	Sequence		Length	Mass (Da)
	P07291 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: C34640DDE2A27386	FASTA	157	17,748
	10 20 30 40 50 60 MPKLSQDEID DLKDVFELFD FWDGRDGAVD AFKLGDVCRC LGINPRNEDV FAVGGTHKMG 70 80 90 100 110 120 EKSLPFEEFL PAYEGLMDCE QGTFADYMEA FKTFDREGQG FISGAELRHV LTALGERLSD 130 140 150 EDVDEIIKLT DLQEDLEGNV KYEDFVKKVM AGPYPDK

References

[1]	"Cloning and characterization of the scallop essential and regulatory myosin light chain cDNAs." Goodwin E.B., Szent-Gyorgyi A.G., Leinwand L.A. J. Biol. Chem. 262:11052-11056(1987) [PubMed: 2440882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Amino acid sequence of myosin essential light chain from the scallop Aquipecten irradians." Collins J.H., Jakes R., Kendrick-Jones J., Leszyk J., Barouch W., Theibert J.L., Spiegel J., Szent-Gyorgyi A.G. Biochemistry 25:7651-7656(1986) [PubMed: 3801438] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-157.
[3]	"Structure of the regulatory domain of scallop myosin at 2.8-A resolution." Xie X., Harrison D.H., Schlichting I., Sweet R.M., Kalabokis V.N., Szent-Gyorgyi A.G., Cohen C. Nature 368:306-312(1994) [PubMed: 8127365] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
[4]	"Structure of the regulatory domain of scallop myosin at 2-A resolution: implications for regulation." Houdusse A., Cohen C. Structure 4:21-32(1996) [PubMed: 8805510] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M17201 mRNA. Translation: AAA27714.1.

PIR

A25183.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B7T	X-ray	2.50	Z	2-157	[»]
1DFK	X-ray	4.20	Z	4-155	[»]
1DFL	X-ray	4.20	X/Z	4-155	[»]
1KK7	X-ray	3.20	Z	2-156	[»]
1KK8	X-ray	2.30	C	2-154	[»]
1KQM	X-ray	3.00	C	2-156	[»]
1KWO	X-ray	3.80	C	2-156	[»]
1L2O	X-ray	2.80	C	2-156	[»]
1QVI	X-ray	2.54	Z	2-156	[»]
1S5G	X-ray	3.10	Z	2-156	[»]
1SCM	X-ray	2.80	C	5-153	[»]
1SR6	X-ray	2.75	C	2-156	[»]
1WDC	X-ray	2.00	C	2-157	[»]
2W4T	electron microscopy	35.00	Z	5-155	[»]
2W4V	electron microscopy	35.00	Z	5-155	[»]
2W4W	electron microscopy	35.00	Z	5-155	[»]
3JTD	X-ray	2.57	C	2-157	[»]
3JVT	X-ray	2.10	C	2-157	[»]

ProteinModelPortal

P07291.

SMR

P07291. Positions 2-156.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

InterPro

IPR011992. EF-hand-like_dom.
IPR018249. EF_HAND_2.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 2 hits.

PROSITE

PS50222. EF_HAND_2. 2 hits.
[Graphical view]

ProtoNet

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Entry information

Entry name

MLE_AEQIR

Accession

Primary (citable) accession number: P07291

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	June 28, 2011
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)