Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

rRNA adenine N-6-methyltransferase

Gene

ermE

Organism
Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This protein produces a dimethylation of the adenine residue at position 2085 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics.

Catalytic activityi

2 S-adenosyl-L-methionine + adenine(2085) in 23S rRNA = 2 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(2085) in 23S rRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei44 – 441S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Binding sitei69 – 691S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei90 – 901S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei115 – 1151S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei131 – 1311S-adenosyl-L-methioninePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17659.

Names & Taxonomyi

Protein namesi
Recommended name:
rRNA adenine N-6-methyltransferase (EC:2.1.1.184)
Short name:
NMT
Alternative name(s):
Erythromycin resistance protein
Macrolide-lincosamide-streptogramin B resistance protein
Gene namesi
Name:ermE
Ordered Locus Names:SACE_0733
OrganismiSaccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeSaccharopolyspora
Proteomesi
  • UP000006728 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 381381rRNA adenine N-6-methyltransferasePRO_0000101677Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi405948.SeryN2_010100032598.

Structurei

3D structure databases

ProteinModelPortaliP07287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi291 – 37989Arg/Gly-richAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108SM1. Bacteria.
COG0030. LUCA.
HOGENOMiHOG000075970.
KOiK00561.
OMAiYPRRRVE.
OrthoDBiEOG66F08Z.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07287-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSDEQPRP RRRNQDRQHP NQNRPVLGRT ERDRNRRQFG QNFLRDRKTI
60 70 80 90 100
ARIAETAELR PDLPVLEAGP GEGLLTRELA DRARQVTSYE IDPRLAKSLR
110 120 130 140 150
EKLSGHPNIE VVNADFLTAE PPPEPFAFVG AIPYGITSAI VDWCLEAPTI
160 170 180 190 200
ETATMVTQLE FARKRTGDYG RWSRLTVMTW PLFEWEFVEK VDRRLFKPVP
210 220 230 240 250
KVDSAIMRLR RRAEPLLEGA ALERYESMVE LCFTGVGGNI QASLLRKYPR
260 270 280 290 300
RRVEAALDHA GVGGGAVVAY VRPEQWLRLF ERLDQKNEPR GGQPQRGRRT
310 320 330 340 350
GGRDHGDRRT GGQDRGDRRT GGRDHRDRQA SGHGDRRSSG RNRDDGRTGE
360 370 380
REQGDQGGRR GPSGGGRTGG RPGRRGGPGQ R
Length:381
Mass (Da):43,001
Last modified:June 26, 2007 - v2
Checksum:i17A7C78CAD865FA9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711G → V in AAA26492 (PubMed:3934045).Curated
Sequence conflicti191 – 20111Missing in AAA26492 (PubMed:3934045).CuratedAdd
BLAST
Sequence conflicti257 – 2571L → F in AAA26492 (PubMed:3934045).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11200 Genomic DNA. Translation: AAA26492.1.
AM420293 Genomic DNA. Translation: CAM00074.1.
X51891 Genomic DNA. Translation: CAB60001.1.
PIRiA91534. XYSMRE.
RefSeqiWP_009950391.1. NZ_ABFV01000100.1.

Genome annotation databases

EnsemblBacteriaiCAM00074; CAM00074; SACE_0733.
KEGGiag:CAB60001.
sen:SACE_0733.
PATRICi23408854. VBISacEry28377_0747.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11200 Genomic DNA. Translation: AAA26492.1.
AM420293 Genomic DNA. Translation: CAM00074.1.
X51891 Genomic DNA. Translation: CAB60001.1.
PIRiA91534. XYSMRE.
RefSeqiWP_009950391.1. NZ_ABFV01000100.1.

3D structure databases

ProteinModelPortaliP07287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405948.SeryN2_010100032598.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM00074; CAM00074; SACE_0733.
KEGGiag:CAB60001.
sen:SACE_0733.
PATRICi23408854. VBISacEry28377_0747.

Phylogenomic databases

eggNOGiENOG4108SM1. Bacteria.
COG0030. LUCA.
HOGENOMiHOG000075970.
KOiK00561.
OMAiYPRRRVE.
OrthoDBiEOG66F08Z.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17659.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "N-methyl transferase of Streptomyces erythraeus that confers resistance to the macrolide-lincosamide-streptogramin B antibiotics: amino acid sequence and its homology to cognate R-factor enzymes from pathogenic bacilli and cocci."
    Uchiyama H., Weisblum B.
    Gene 38:103-110(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A repeated decapeptide motif in the C-terminal domain of the ribosomal RNA methyltransferase from the erythromycin producer Saccharopolyspora erythraea."
    Dhillon N., Leadlay P.F.
    FEBS Lett. 262:189-193(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
    Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
    Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338.
  4. "Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus."
    Bibb M.J., Janssen G.R., Ward J.M.
    Gene 38:215-226(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-133.

Entry informationi

Entry nameiERME_SACEN
AccessioniPrimary (citable) accession number: P07287
Secondary accession number(s): A4F7Q0, Q54094
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: June 26, 2007
Last modified: November 11, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.