Reviewed, UniProtKB/Swiss-Prot P07287 (ERME_SACEN)

Names and origin

Protein names

Recommended name:
    rRNA adenine N-6-methyltransferase
      Short name=NMT
    EC=2.1.1.48
Alternative name(s):
    Macrolide-lincosamide-streptogramin B resistance protein
    Erythromycin resistance protein

Gene names

Name:	ermE
Ordered Locus Names:	SACE_0733

Organism

Saccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]

Taxonomic identifier

405948 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Saccharopolyspora

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein produces a dimethylation of the adenine residue at position 2058 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine.
Sequence similarities	Belongs to the rRNA adenine N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA modification Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA (adenine-N6,N6-)-dimethyltransferase activity Inferred from electronic annotation. Source: InterPro rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	rRNA adenine N-6-methyltransferase		PRO_0000101677
Regions
	Compositional bias	291 – 379	89	Arg/Gly-rich
Experimental info
	Sequence conflict	71	1	G → V in AAA26492. Ref.1
	Sequence conflict	191 – 201	11	Missing in AAA26492. Ref.1
	Sequence conflict	257	1	L → F in AAA26492. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P07287-1 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: 17A7C78CAD865FA9	FASTA	381	43,001
	10 20 30 40 50 60 MSSSDEQPRP RRRNQDRQHP NQNRPVLGRT ERDRNRRQFG QNFLRDRKTI ARIAETAELR 70 80 90 100 110 120 PDLPVLEAGP GEGLLTRELA DRARQVTSYE IDPRLAKSLR EKLSGHPNIE VVNADFLTAE 130 140 150 160 170 180 PPPEPFAFVG AIPYGITSAI VDWCLEAPTI ETATMVTQLE FARKRTGDYG RWSRLTVMTW 190 200 210 220 230 240 PLFEWEFVEK VDRRLFKPVP KVDSAIMRLR RRAEPLLEGA ALERYESMVE LCFTGVGGNI 250 260 270 280 290 300 QASLLRKYPR RRVEAALDHA GVGGGAVVAY VRPEQWLRLF ERLDQKNEPR GGQPQRGRRT 310 320 330 340 350 360 GGRDHGDRRT GGQDRGDRRT GGRDHRDRQA SGHGDRRSSG RNRDDGRTGE REQGDQGGRR 370 380 GPSGGGRTGG RPGRRGGPGQ R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"N-methyl transferase of Streptomyces erythraeus that confers resistance to the macrolide-lincosamide-streptogramin B antibiotics: amino acid sequence and its homology to cognate R-factor enzymes from pathogenic bacilli and cocci." Uchiyama H., Weisblum B. Gene 38:103-110(1985) [PubMed: 3934045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A repeated decapeptide motif in the C-terminal domain of the ribosomal RNA methyltransferase from the erythromycin producer Saccharopolyspora erythraea." Dhillon N., Leadlay P.F. FEBS Lett. 262:189-193(1990) [PubMed: 2335200] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338." Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F. Nat. Biotechnol. 25:447-453(2007) [PubMed: 17369815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus." Bibb M.J., Janssen G.R., Ward J.M. Gene 38:215-226(1985) [PubMed: 2998943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-133.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M11200 Genomic DNA. Translation: AAA26492.1. AM420293 Genomic DNA. Translation: CAM00074.1. X51891 Genomic DNA. Translation: CAB60001.1.
PIR	XYSMRE. A91534.
RefSeq	YP_001103000.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4940594.
GenomeReviews	Gene locus SACE_0733 in contig AM420293_GR.
KEGG	sen:SACE_0733.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	P07287. IERRREP.
Family and domain databases
InterPro	IPR001737. RRNA_Ade_Mease_Trfase. [Graphical view]
Pfam	PF00398. RrnaAD. 1 hit. [Graphical view]
PROSITE	PS01131. RRNA_A_DIMETH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERME_SACEN

Accession

Primary (citable) accession number: P07287
Secondary accession number(s): A4F7Q0, Q54094

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	June 26, 2007
Last modified:	May 5, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)