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Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene

ALG7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

Enzyme regulationi

Inhibited by tunicamycin.

Pathway: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • aerobic respiration Source: SGD
  • protein N-linked glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-7122.
YEAST:YBR243C-MONOMER.
ReactomeiREACT_299009. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
Alternative name(s):
GlcNAc-1-P transferase
Short name:
G1PT
Short name:
GPT
N-acetylglucosamine-1-phosphate transferase
Tunicamycin resistance protein 1
Gene namesi
Name:ALG7
Synonyms:TUR1
Ordered Locus Names:YBR243C
ORF Names:YBR1628
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome II

Organism-specific databases

CYGDiYBR243c.
EuPathDBiFungiDB:YBR243C.
SGDiS000000447. ALG7.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei24 – 4421HelicalSequence AnalysisAdd
BLAST
Transmembranei72 – 9221HelicalSequence AnalysisAdd
BLAST
Transmembranei129 – 14921HelicalSequence AnalysisAdd
BLAST
Transmembranei157 – 17721HelicalSequence AnalysisAdd
BLAST
Transmembranei202 – 22221HelicalSequence AnalysisAdd
BLAST
Transmembranei231 – 25121HelicalSequence AnalysisAdd
BLAST
Transmembranei256 – 27621HelicalSequence AnalysisAdd
BLAST
Transmembranei283 – 30321HelicalSequence AnalysisAdd
BLAST
Transmembranei309 – 32921HelicalSequence AnalysisAdd
BLAST
Transmembranei387 – 40721HelicalSequence AnalysisAdd
BLAST
Transmembranei419 – 43921HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • UDP-N-acetylglucosamine transferase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 448448UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferasePRO_0000108765Add
BLAST

Proteomic databases

MaxQBiP07286.

Interactioni

Protein-protein interaction databases

BioGridi32938. 43 interactions.
DIPiDIP-5364N.
IntActiP07286. 7 interactions.
MINTiMINT-536736.
STRINGi4932.YBR243C.

Structurei

3D structure databases

ProteinModelPortaliP07286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi81 – 9313Dolichol recognitionAdd
BLAST
Motifi257 – 26913Dolichol recognitionAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0472.
GeneTreeiENSGT00390000011424.
HOGENOMiHOG000163915.
InParanoidiP07286.
KOiK01001.
OMAiIHERNLT.
OrthoDBiEOG7380DT.

Family and domain databases

InterProiIPR000715. Glycosyl_transferase_4.
[Graphical view]
PfamiPF00953. Glycos_transf_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07286-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRLFSLALI TCLIYYSKNQ GPSALVAAVG FGIAGYLATD MLIPRVGKSF
60 70 80 90 100
IKIGLFGKDL SKPGRPVLPE TIGAIPAAVY LFVMFIYIPF IFYKYMVITT
110 120 130 140 150
SGGGHRDVSV VEDNGMNSNI FPHDKLSEYL SAILCLESTV LLGIADDLFD
160 170 180 190 200
LRWRHKFFLP AIAAIPLLMV YYVDFGVTHV LIPGFMERWL KKTSVDLGLW
210 220 230 240 250
YYVYMASMAI FCPNSINILA GVNGLEVGQC IVLAILALLN DLLYFSMGPL
260 270 280 290 300
ATRDSHRFSA VLIIPFLGVS LALWKWNRWP ATVFVGDTYC YFAGMVFAVV
310 320 330 340 350
GILGHFSKTM LLLFIPQIVN FIYSCPQLFK LVPCPRHRLP KFNEKDGLMY
360 370 380 390 400
PSRANLKEEP PKSIFKPILK LLYCLHLIDL EFDENNEIIS TSNMTLINLT
410 420 430 440
LVWFGPMRED KLCNTILKLQ FCIGILALLG RHAIGAIIFG HDNLWTVR
Length:448
Mass (Da):50,368
Last modified:April 1, 1988 - v1
Checksum:i00A617D831335637
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00126 Genomic DNA. Translation: CAA68324.1.
Z36112 Genomic DNA. Translation: CAA85206.1.
BK006936 Genomic DNA. Translation: DAA07359.1.
PIRiA27522.
RefSeqiNP_009802.3. NM_001178591.3.

Genome annotation databases

EnsemblFungiiYBR243C; YBR243C; YBR243C.
GeneIDi852545.
KEGGisce:YBR243C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00126 Genomic DNA. Translation: CAA68324.1.
Z36112 Genomic DNA. Translation: CAA85206.1.
BK006936 Genomic DNA. Translation: DAA07359.1.
PIRiA27522.
RefSeqiNP_009802.3. NM_001178591.3.

3D structure databases

ProteinModelPortaliP07286.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32938. 43 interactions.
DIPiDIP-5364N.
IntActiP07286. 7 interactions.
MINTiMINT-536736.
STRINGi4932.YBR243C.

Proteomic databases

MaxQBiP07286.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR243C; YBR243C; YBR243C.
GeneIDi852545.
KEGGisce:YBR243C.

Organism-specific databases

CYGDiYBR243c.
EuPathDBiFungiDB:YBR243C.
SGDiS000000447. ALG7.

Phylogenomic databases

eggNOGiCOG0472.
GeneTreeiENSGT00390000011424.
HOGENOMiHOG000163915.
InParanoidiP07286.
KOiK01001.
OMAiIHERNLT.
OrthoDBiEOG7380DT.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:MONOMER-7122.
YEAST:YBR243C-MONOMER.
ReactomeiREACT_299009. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

NextBioi971622.
PROiP07286.

Family and domain databases

InterProiIPR000715. Glycosyl_transferase_4.
[Graphical view]
PfamiPF00953. Glycos_transf_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic sequence coding for tunicamycin resistance in yeast."
    Hartog K.O., Bishop B.
    Nucleic Acids Res. 15:3627-3627(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiGPT_YEAST
AccessioniPrimary (citable) accession number: P07286
Secondary accession number(s): D6VQN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: June 24, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.