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P07286 (GPT_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
EC=2.7.8.15
Alternative name(s):
GlcNAc-1-P transferase
Short name=G1PT
Short name=GPT
N-acetylglucosamine-1-phosphate transferase
Tunicamycin resistance protein 1
Gene names
Name:ALG7
Synonyms:TUR1
Ordered Locus Names:YBR243C
ORF Names:YBR1628
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length448 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

Catalytic activity

UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

Enzyme regulation

Inhibited by tunicamycin.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the glycosyltransferase 4 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 448448UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
PRO_0000108765

Regions

Transmembrane24 – 4421Helical; Potential
Transmembrane72 – 9221Helical; Potential
Transmembrane129 – 14921Helical; Potential
Transmembrane157 – 17721Helical; Potential
Transmembrane202 – 22221Helical; Potential
Transmembrane231 – 25121Helical; Potential
Transmembrane256 – 27621Helical; Potential
Transmembrane283 – 30321Helical; Potential
Transmembrane309 – 32921Helical; Potential
Transmembrane387 – 40721Helical; Potential
Transmembrane419 – 43921Helical; Potential
Motif81 – 9313Dolichol recognition
Motif257 – 26913Dolichol recognition

Sequences

Sequence LengthMass (Da)Tools
P07286 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 00A617D831335637

FASTA44850,368
        10         20         30         40         50         60 
MLRLFSLALI TCLIYYSKNQ GPSALVAAVG FGIAGYLATD MLIPRVGKSF IKIGLFGKDL 

        70         80         90        100        110        120 
SKPGRPVLPE TIGAIPAAVY LFVMFIYIPF IFYKYMVITT SGGGHRDVSV VEDNGMNSNI 

       130        140        150        160        170        180 
FPHDKLSEYL SAILCLESTV LLGIADDLFD LRWRHKFFLP AIAAIPLLMV YYVDFGVTHV 

       190        200        210        220        230        240 
LIPGFMERWL KKTSVDLGLW YYVYMASMAI FCPNSINILA GVNGLEVGQC IVLAILALLN 

       250        260        270        280        290        300 
DLLYFSMGPL ATRDSHRFSA VLIIPFLGVS LALWKWNRWP ATVFVGDTYC YFAGMVFAVV 

       310        320        330        340        350        360 
GILGHFSKTM LLLFIPQIVN FIYSCPQLFK LVPCPRHRLP KFNEKDGLMY PSRANLKEEP 

       370        380        390        400        410        420 
PKSIFKPILK LLYCLHLIDL EFDENNEIIS TSNMTLINLT LVWFGPMRED KLCNTILKLQ 

       430        440 
FCIGILALLG RHAIGAIIFG HDNLWTVR

« Hide

References

« Hide 'large scale' references
[1]"Genomic sequence coding for tunicamycin resistance in yeast."
Hartog K.O., Bishop B.
Nucleic Acids Res. 15:3627-3627(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Y00126 Genomic DNA. Translation: CAA68324.1.
Z36112 Genomic DNA. Translation: CAA85206.1.
BK006936 Genomic DNA. Translation: DAA07359.1.
PIRA27522.
RefSeqNP_009802.3. NM_001178591.3.

3D structure databases

ProteinModelPortalP07286.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-5364N.
IntActP07286. 5 interactions.
MINTMINT-536736.
STRING4932.YBR243C.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR243C; YBR243C; YBR243C.
GeneID852545.
KEGGsce:YBR243C.
sce:YBR247C.

Organism-specific databases

CYGDYBR243c.
SGDS000000447. ALG7.

Phylogenomic databases

eggNOGCOG0472.
GeneTreeENSGT00390000011424.
HOGENOMHOG000163915.
KOK01001.
K14797.
OMAYLAFVHY.
OrthoDBEOG4BK8CS.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-7122.
UniPathwayUPA00378.

Gene expression databases

GenevestigatorP07286.
GermOnlineYBR243C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR000715. Glycosyl_transferase_4.
[Graphical view]
PfamPF00953. Glycos_transf_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio971622.

Entry information

Entry nameGPT_YEAST
AccessionPrimary (citable) accession number: P07286
Secondary accession number(s): D6VQN9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: April 3, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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