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P07286

- GPT_YEAST

UniProt

P07286 - GPT_YEAST

Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene

ALG7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

    Enzyme regulationi

    Inhibited by tunicamycin.

    Pathwayi

    GO - Molecular functioni

    1. phospho-N-acetylmuramoyl-pentapeptide-transferase activity Source: InterPro
    2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW
    3. UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity Source: SGD

    GO - Biological processi

    1. aerobic respiration Source: SGD
    2. protein N-linked glycosylation Source: SGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-7122.
    YEAST:YBR243C-MONOMER.
    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
    Alternative name(s):
    GlcNAc-1-P transferase
    Short name:
    G1PT
    Short name:
    GPT
    N-acetylglucosamine-1-phosphate transferase
    Tunicamycin resistance protein 1
    Gene namesi
    Name:ALG7
    Synonyms:TUR1
    Ordered Locus Names:YBR243C
    ORF Names:YBR1628
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR243c.
    SGDiS000000447. ALG7.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. UDP-N-acetylglucosamine transferase complex Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 448448UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferasePRO_0000108765Add
    BLAST

    Proteomic databases

    MaxQBiP07286.

    Expressioni

    Gene expression databases

    GenevestigatoriP07286.

    Interactioni

    Protein-protein interaction databases

    BioGridi32938. 43 interactions.
    DIPiDIP-5364N.
    IntActiP07286. 7 interactions.
    MINTiMINT-536736.
    STRINGi4932.YBR243C.

    Structurei

    3D structure databases

    ProteinModelPortaliP07286.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei24 – 4421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei72 – 9221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei129 – 14921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei157 – 17721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei202 – 22221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei231 – 25121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei256 – 27621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei283 – 30321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei309 – 32921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei387 – 40721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei419 – 43921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi81 – 9313Dolichol recognitionAdd
    BLAST
    Motifi257 – 26913Dolichol recognitionAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyltransferase 4 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0472.
    GeneTreeiENSGT00390000011424.
    HOGENOMiHOG000163915.
    KOiK01001.
    OMAiIHERNLT.
    OrthoDBiEOG7380DT.

    Family and domain databases

    InterProiIPR000715. Glycosyl_transferase_4.
    [Graphical view]
    PfamiPF00953. Glycos_transf_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07286-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRLFSLALI TCLIYYSKNQ GPSALVAAVG FGIAGYLATD MLIPRVGKSF    50
    IKIGLFGKDL SKPGRPVLPE TIGAIPAAVY LFVMFIYIPF IFYKYMVITT 100
    SGGGHRDVSV VEDNGMNSNI FPHDKLSEYL SAILCLESTV LLGIADDLFD 150
    LRWRHKFFLP AIAAIPLLMV YYVDFGVTHV LIPGFMERWL KKTSVDLGLW 200
    YYVYMASMAI FCPNSINILA GVNGLEVGQC IVLAILALLN DLLYFSMGPL 250
    ATRDSHRFSA VLIIPFLGVS LALWKWNRWP ATVFVGDTYC YFAGMVFAVV 300
    GILGHFSKTM LLLFIPQIVN FIYSCPQLFK LVPCPRHRLP KFNEKDGLMY 350
    PSRANLKEEP PKSIFKPILK LLYCLHLIDL EFDENNEIIS TSNMTLINLT 400
    LVWFGPMRED KLCNTILKLQ FCIGILALLG RHAIGAIIFG HDNLWTVR 448
    Length:448
    Mass (Da):50,368
    Last modified:April 1, 1988 - v1
    Checksum:i00A617D831335637
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00126 Genomic DNA. Translation: CAA68324.1.
    Z36112 Genomic DNA. Translation: CAA85206.1.
    BK006936 Genomic DNA. Translation: DAA07359.1.
    PIRiA27522.
    RefSeqiNP_009802.3. NM_001178591.3.

    Genome annotation databases

    EnsemblFungiiYBR243C; YBR243C; YBR243C.
    GeneIDi852545.
    KEGGisce:YBR243C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00126 Genomic DNA. Translation: CAA68324.1 .
    Z36112 Genomic DNA. Translation: CAA85206.1 .
    BK006936 Genomic DNA. Translation: DAA07359.1 .
    PIRi A27522.
    RefSeqi NP_009802.3. NM_001178591.3.

    3D structure databases

    ProteinModelPortali P07286.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32938. 43 interactions.
    DIPi DIP-5364N.
    IntActi P07286. 7 interactions.
    MINTi MINT-536736.
    STRINGi 4932.YBR243C.

    Proteomic databases

    MaxQBi P07286.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR243C ; YBR243C ; YBR243C .
    GeneIDi 852545.
    KEGGi sce:YBR243C.

    Organism-specific databases

    CYGDi YBR243c.
    SGDi S000000447. ALG7.

    Phylogenomic databases

    eggNOGi COG0472.
    GeneTreei ENSGT00390000011424.
    HOGENOMi HOG000163915.
    KOi K01001.
    OMAi IHERNLT.
    OrthoDBi EOG7380DT.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BioCyci MetaCyc:MONOMER-7122.
    YEAST:YBR243C-MONOMER.

    Miscellaneous databases

    NextBioi 971622.
    PROi P07286.

    Gene expression databases

    Genevestigatori P07286.

    Family and domain databases

    InterProi IPR000715. Glycosyl_transferase_4.
    [Graphical view ]
    Pfami PF00953. Glycos_transf_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequence coding for tunicamycin resistance in yeast."
      Hartog K.O., Bishop B.
      Nucleic Acids Res. 15:3627-3627(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.

    Entry informationi

    Entry nameiGPT_YEAST
    AccessioniPrimary (citable) accession number: P07286
    Secondary accession number(s): D6VQN9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3