SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P07284

- SYSC_YEAST

UniProt

P07284 - SYSC_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Serine--tRNA ligase, cytoplasmic
Gene
SES1, SERS, YDR023W, YD9813.01
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).1 Publication

Catalytic activityi

ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser).
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei295 – 2951ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei302 – 3021Serine By similarity
Binding sitei404 – 4041Serine By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi279 – 2813ATP By similarity
Nucleotide bindingi366 – 3694ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. serine-tRNA ligase activity Source: SGD

GO - Biological processi

  1. selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway
  2. seryl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29640-MONOMER.
UniPathwayiUPA00906; UER00895.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine--tRNA ligase, cytoplasmic (EC:6.1.1.11)
Alternative name(s):
Seryl-tRNA synthetase
Short name:
SerRS
Seryl-tRNA(Ser/Sec) synthetase
Gene namesi
Name:SES1
Synonyms:SERS
Ordered Locus Names:YDR023W
ORF Names:YD9813.01
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR023w.
SGDiS000002430. SES1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Serine--tRNA ligase, cytoplasmic
PRO_0000122199Add
BLAST

Proteomic databases

MaxQBiP07284.
PaxDbiP07284.
PeptideAtlasiP07284.
PRIDEiP07284.

Expressioni

Gene expression databases

GenevestigatoriP07284.

Interactioni

Subunit structurei

Homodimer. The tRNA molecule probably binds across the dimer.1 Publication

Protein-protein interaction databases

BioGridi32074. 46 interactions.
DIPiDIP-5181N.
IntActiP07284. 3 interactions.
MINTiMINT-513156.
STRINGi4932.YDR023W.

Structurei

3D structure databases

ProteinModelPortaliP07284.
SMRiP07284. Positions 1-451.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni246 – 2483Serine binding By similarity

Domaini

Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0172.
GeneTreeiENSGT00750000117703.
HOGENOMiHOG000035937.
KOiK01875.
OMAiINTRINQ.
OrthoDBiEOG7PVWZM.

Family and domain databases

Gene3Di1.10.287.40. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-ligase_type_1.
IPR015866. Ser-tRNA-synth_1_N.
IPR010978. tRNA-bd_arm.
[Graphical view]
PANTHERiPTHR11778. PTHR11778. 1 hit.
PfamiPF02403. Seryl_tRNA_N. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PIRSFiPIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTSiPR00981. TRNASYNTHSER.
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00414. serS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07284-1 [UniParc]FASTAAdd to Basket

« Hide

MLDINQFIED KGGNPELIRQ SQKARNASVE IVDEIISDYK DWVKTRFELD    50
ELNKKFNKLQ KDIGLKFKNK EDASGLLAEK EKLTQQKKEL TEKEQQEDKD 100
LKKKVFQVGN IVHPSVVVSN DEENNELVRT WKPEDLEAVG PIASVTGKPA 150
SLSHHEILLR LDGYDPDRGV KICGHRGYFF RNYGVFLNQA LINYGLQFLA 200
AKGYIPLQAP VMMNKELMSK TAQLSEFDEE LYKVIDGEDE KYLIATSEQP 250
ISAYHSGEWF EKPQEQLPIH YVGYSSCFRR EAGSHGKDAW GVFRVHAFEK 300
IEQFVITEPE KSWEEFEKMI SYSEEFYKSL KLPYRIVGIV SGELNNAAAK 350
KYDLEAWFPY QKEYKELVSC SNCTDYQSRN LEIRCGIKKM GDREKKYVHC 400
LNSTLAATQR ALCCILENYQ TEDGLVVPEV LRKYIPGEPE FLPFVNELPK 450
NSTSSKDKKK KN 462
Length:462
Mass (Da):53,310
Last modified:October 1, 1996 - v2
Checksum:iBBED6EA47E6F547B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti224 – 2241L → P in CAA28572. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04884 Genomic DNA. Translation: CAA28572.1.
Z47814 Genomic DNA. Translation: CAA87802.1.
X95966 Genomic DNA. Translation: CAA65216.1.
Z74319 Genomic DNA. Translation: CAA98844.1.
L15408 Genomic DNA. Translation: AAC37412.1.
BK006938 Genomic DNA. Translation: DAA11869.1.
PIRiS50930. YSBYC.
RefSeqiNP_010306.1. NM_001180331.1.

Genome annotation databases

EnsemblFungiiYDR023W; YDR023W; YDR023W.
GeneIDi851587.
KEGGisce:YDR023W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04884 Genomic DNA. Translation: CAA28572.1 .
Z47814 Genomic DNA. Translation: CAA87802.1 .
X95966 Genomic DNA. Translation: CAA65216.1 .
Z74319 Genomic DNA. Translation: CAA98844.1 .
L15408 Genomic DNA. Translation: AAC37412.1 .
BK006938 Genomic DNA. Translation: DAA11869.1 .
PIRi S50930. YSBYC.
RefSeqi NP_010306.1. NM_001180331.1.

3D structure databases

ProteinModelPortali P07284.
SMRi P07284. Positions 1-451.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32074. 46 interactions.
DIPi DIP-5181N.
IntActi P07284. 3 interactions.
MINTi MINT-513156.
STRINGi 4932.YDR023W.

Proteomic databases

MaxQBi P07284.
PaxDbi P07284.
PeptideAtlasi P07284.
PRIDEi P07284.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR023W ; YDR023W ; YDR023W .
GeneIDi 851587.
KEGGi sce:YDR023W.

Organism-specific databases

CYGDi YDR023w.
SGDi S000002430. SES1.

Phylogenomic databases

eggNOGi COG0172.
GeneTreei ENSGT00750000117703.
HOGENOMi HOG000035937.
KOi K01875.
OMAi INTRINQ.
OrthoDBi EOG7PVWZM.

Enzyme and pathway databases

UniPathwayi UPA00906 ; UER00895 .
BioCyci YEAST:G3O-29640-MONOMER.

Miscellaneous databases

NextBioi 969063.
PROi P07284.

Gene expression databases

Genevestigatori P07284.

Family and domain databases

Gene3Di 1.10.287.40. 1 hit.
InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-ligase_type_1.
IPR015866. Ser-tRNA-synth_1_N.
IPR010978. tRNA-bd_arm.
[Graphical view ]
PANTHERi PTHR11778. PTHR11778. 1 hit.
Pfami PF02403. Seryl_tRNA_N. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view ]
PIRSFi PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTSi PR00981. TRNASYNTHSER.
SUPFAMi SSF46589. SSF46589. 1 hit.
TIGRFAMsi TIGR00414. serS. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the gene coding for cytoplasmic seryl-tRNA synthetase from Saccharomyces cerevisiae."
    Weygand-Durasevic I., Johnson-Burke D., Soell D.
    Nucleic Acids Res. 15:1887-1904(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT.
  2. "Sequencing and analysis of a 35.4 kb region on the right arm of chromosome IV from Saccharomyces cerevisiae reveal 23 open reading frames."
    Eide L.G., Sander C., Prydz H.
    Yeast 12:1085-1090(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Reduced dosage of genes encoding ribosomal protein S18 suppresses a mitochondrial initiation codon mutation in Saccharomyces cerevisiae."
    Folley L.S., Fox T.D.
    Genetics 137:369-379(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 451-462.
    Strain: ATCC 204508 / S288c.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYSC_YEAST
AccessioniPrimary (citable) accession number: P07284
Secondary accession number(s): D6VS09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi