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P07284

- SYSC_YEAST

UniProt

P07284 - SYSC_YEAST

Protein

Serine--tRNA ligase, cytoplasmic

Gene

SES1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).1 Publication

    Catalytic activityi

    ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser).
    ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei295 – 2951ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei302 – 3021SerineBy similarity
    Binding sitei404 – 4041SerineBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi279 – 2813ATPBy similarity
    Nucleotide bindingi366 – 3694ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. serine-tRNA ligase activity Source: SGD

    GO - Biological processi

    1. selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway
    2. seryl-tRNA aminoacylation Source: SGD

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29640-MONOMER.
    UniPathwayiUPA00906; UER00895.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine--tRNA ligase, cytoplasmic (EC:6.1.1.11)
    Alternative name(s):
    Seryl-tRNA synthetase
    Short name:
    SerRS
    Seryl-tRNA(Ser/Sec) synthetase
    Gene namesi
    Name:SES1
    Synonyms:SERS
    Ordered Locus Names:YDR023W
    ORF Names:YD9813.01
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR023w.
    SGDiS000002430. SES1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 462462Serine--tRNA ligase, cytoplasmicPRO_0000122199Add
    BLAST

    Proteomic databases

    MaxQBiP07284.
    PaxDbiP07284.
    PeptideAtlasiP07284.
    PRIDEiP07284.

    Expressioni

    Gene expression databases

    GenevestigatoriP07284.

    Interactioni

    Subunit structurei

    Homodimer. The tRNA molecule probably binds across the dimer.1 Publication

    Protein-protein interaction databases

    BioGridi32074. 46 interactions.
    DIPiDIP-5181N.
    IntActiP07284. 3 interactions.
    MINTiMINT-513156.
    STRINGi4932.YDR023W.

    Structurei

    3D structure databases

    ProteinModelPortaliP07284.
    SMRiP07284. Positions 1-451.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni246 – 2483Serine bindingBy similarity

    Domaini

    Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0172.
    GeneTreeiENSGT00750000117703.
    HOGENOMiHOG000035937.
    KOiK01875.
    OMAiINTRINQ.
    OrthoDBiEOG7PVWZM.

    Family and domain databases

    Gene3Di1.10.287.40. 1 hit.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR002317. Ser-tRNA-ligase_type_1.
    IPR015866. Ser-tRNA-synth_1_N.
    IPR010978. tRNA-bd_arm.
    [Graphical view]
    PANTHERiPTHR11778. PTHR11778. 1 hit.
    PfamiPF02403. Seryl_tRNA_N. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
    PRINTSiPR00981. TRNASYNTHSER.
    SUPFAMiSSF46589. SSF46589. 1 hit.
    TIGRFAMsiTIGR00414. serS. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07284-1 [UniParc]FASTAAdd to Basket

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    MLDINQFIED KGGNPELIRQ SQKARNASVE IVDEIISDYK DWVKTRFELD    50
    ELNKKFNKLQ KDIGLKFKNK EDASGLLAEK EKLTQQKKEL TEKEQQEDKD 100
    LKKKVFQVGN IVHPSVVVSN DEENNELVRT WKPEDLEAVG PIASVTGKPA 150
    SLSHHEILLR LDGYDPDRGV KICGHRGYFF RNYGVFLNQA LINYGLQFLA 200
    AKGYIPLQAP VMMNKELMSK TAQLSEFDEE LYKVIDGEDE KYLIATSEQP 250
    ISAYHSGEWF EKPQEQLPIH YVGYSSCFRR EAGSHGKDAW GVFRVHAFEK 300
    IEQFVITEPE KSWEEFEKMI SYSEEFYKSL KLPYRIVGIV SGELNNAAAK 350
    KYDLEAWFPY QKEYKELVSC SNCTDYQSRN LEIRCGIKKM GDREKKYVHC 400
    LNSTLAATQR ALCCILENYQ TEDGLVVPEV LRKYIPGEPE FLPFVNELPK 450
    NSTSSKDKKK KN 462
    Length:462
    Mass (Da):53,310
    Last modified:October 1, 1996 - v2
    Checksum:iBBED6EA47E6F547B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti224 – 2241L → P in CAA28572. (PubMed:3031581)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04884 Genomic DNA. Translation: CAA28572.1.
    Z47814 Genomic DNA. Translation: CAA87802.1.
    X95966 Genomic DNA. Translation: CAA65216.1.
    Z74319 Genomic DNA. Translation: CAA98844.1.
    L15408 Genomic DNA. Translation: AAC37412.1.
    BK006938 Genomic DNA. Translation: DAA11869.1.
    PIRiS50930. YSBYC.
    RefSeqiNP_010306.1. NM_001180331.1.

    Genome annotation databases

    EnsemblFungiiYDR023W; YDR023W; YDR023W.
    GeneIDi851587.
    KEGGisce:YDR023W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04884 Genomic DNA. Translation: CAA28572.1 .
    Z47814 Genomic DNA. Translation: CAA87802.1 .
    X95966 Genomic DNA. Translation: CAA65216.1 .
    Z74319 Genomic DNA. Translation: CAA98844.1 .
    L15408 Genomic DNA. Translation: AAC37412.1 .
    BK006938 Genomic DNA. Translation: DAA11869.1 .
    PIRi S50930. YSBYC.
    RefSeqi NP_010306.1. NM_001180331.1.

    3D structure databases

    ProteinModelPortali P07284.
    SMRi P07284. Positions 1-451.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32074. 46 interactions.
    DIPi DIP-5181N.
    IntActi P07284. 3 interactions.
    MINTi MINT-513156.
    STRINGi 4932.YDR023W.

    Proteomic databases

    MaxQBi P07284.
    PaxDbi P07284.
    PeptideAtlasi P07284.
    PRIDEi P07284.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR023W ; YDR023W ; YDR023W .
    GeneIDi 851587.
    KEGGi sce:YDR023W.

    Organism-specific databases

    CYGDi YDR023w.
    SGDi S000002430. SES1.

    Phylogenomic databases

    eggNOGi COG0172.
    GeneTreei ENSGT00750000117703.
    HOGENOMi HOG000035937.
    KOi K01875.
    OMAi INTRINQ.
    OrthoDBi EOG7PVWZM.

    Enzyme and pathway databases

    UniPathwayi UPA00906 ; UER00895 .
    BioCyci YEAST:G3O-29640-MONOMER.

    Miscellaneous databases

    NextBioi 969063.
    PROi P07284.

    Gene expression databases

    Genevestigatori P07284.

    Family and domain databases

    Gene3Di 1.10.287.40. 1 hit.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR002317. Ser-tRNA-ligase_type_1.
    IPR015866. Ser-tRNA-synth_1_N.
    IPR010978. tRNA-bd_arm.
    [Graphical view ]
    PANTHERi PTHR11778. PTHR11778. 1 hit.
    Pfami PF02403. Seryl_tRNA_N. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
    PRINTSi PR00981. TRNASYNTHSER.
    SUPFAMi SSF46589. SSF46589. 1 hit.
    TIGRFAMsi TIGR00414. serS. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the gene coding for cytoplasmic seryl-tRNA synthetase from Saccharomyces cerevisiae."
      Weygand-Durasevic I., Johnson-Burke D., Soell D.
      Nucleic Acids Res. 15:1887-1904(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT.
    2. "Sequencing and analysis of a 35.4 kb region on the right arm of chromosome IV from Saccharomyces cerevisiae reveal 23 open reading frames."
      Eide L.G., Sander C., Prydz H.
      Yeast 12:1085-1090(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Reduced dosage of genes encoding ribosomal protein S18 suppresses a mitochondrial initiation codon mutation in Saccharomyces cerevisiae."
      Folley L.S., Fox T.D.
      Genetics 137:369-379(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 451-462.
      Strain: ATCC 204508 / S288c.
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYSC_YEAST
    AccessioniPrimary (citable) accession number: P07284
    Secondary accession number(s): D6VS09
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 142 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3