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Protein

Serine--tRNA ligase, cytoplasmic

Gene

SES1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).1 Publication

Catalytic activityi

ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser).
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei295 – 2951ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei302 – 3021SerineBy similarity
Binding sitei404 – 4041SerineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi279 – 2813ATPBy similarity
Nucleotide bindingi366 – 3694ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. serine-tRNA ligase activity Source: SGD

GO - Biological processi

  1. selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway
  2. seryl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29640-MONOMER.
UniPathwayiUPA00906; UER00895.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine--tRNA ligase, cytoplasmic (EC:6.1.1.11)
Alternative name(s):
Seryl-tRNA synthetase
Short name:
SerRS
Seryl-tRNA(Ser/Sec) synthetase
Gene namesi
Name:SES1
Synonyms:SERS
Ordered Locus Names:YDR023W
ORF Names:YD9813.01
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

CYGDiYDR023w.
SGDiS000002430. SES1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Serine--tRNA ligase, cytoplasmicPRO_0000122199Add
BLAST

Proteomic databases

MaxQBiP07284.
PaxDbiP07284.
PeptideAtlasiP07284.
PRIDEiP07284.

Expressioni

Gene expression databases

GenevestigatoriP07284.

Interactioni

Subunit structurei

Homodimer. The tRNA molecule probably binds across the dimer.1 Publication

Protein-protein interaction databases

BioGridi32074. 47 interactions.
DIPiDIP-5181N.
IntActiP07284. 3 interactions.
MINTiMINT-513156.
STRINGi4932.YDR023W.

Structurei

3D structure databases

ProteinModelPortaliP07284.
SMRiP07284. Positions 1-451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni246 – 2483Serine bindingBy similarity

Domaini

Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0172.
GeneTreeiENSGT00780000121981.
HOGENOMiHOG000035937.
InParanoidiP07284.
KOiK01875.
OMAiVEAKMEY.
OrthoDBiEOG7PVWZM.

Family and domain databases

Gene3Di1.10.287.40. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-ligase_type_1.
IPR015866. Ser-tRNA-synth_1_N.
IPR010978. tRNA-bd_arm.
[Graphical view]
PANTHERiPTHR11778. PTHR11778. 1 hit.
PfamiPF02403. Seryl_tRNA_N. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PIRSFiPIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTSiPR00981. TRNASYNTHSER.
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00414. serS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07284-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDINQFIED KGGNPELIRQ SQKARNASVE IVDEIISDYK DWVKTRFELD
60 70 80 90 100
ELNKKFNKLQ KDIGLKFKNK EDASGLLAEK EKLTQQKKEL TEKEQQEDKD
110 120 130 140 150
LKKKVFQVGN IVHPSVVVSN DEENNELVRT WKPEDLEAVG PIASVTGKPA
160 170 180 190 200
SLSHHEILLR LDGYDPDRGV KICGHRGYFF RNYGVFLNQA LINYGLQFLA
210 220 230 240 250
AKGYIPLQAP VMMNKELMSK TAQLSEFDEE LYKVIDGEDE KYLIATSEQP
260 270 280 290 300
ISAYHSGEWF EKPQEQLPIH YVGYSSCFRR EAGSHGKDAW GVFRVHAFEK
310 320 330 340 350
IEQFVITEPE KSWEEFEKMI SYSEEFYKSL KLPYRIVGIV SGELNNAAAK
360 370 380 390 400
KYDLEAWFPY QKEYKELVSC SNCTDYQSRN LEIRCGIKKM GDREKKYVHC
410 420 430 440 450
LNSTLAATQR ALCCILENYQ TEDGLVVPEV LRKYIPGEPE FLPFVNELPK
460
NSTSSKDKKK KN
Length:462
Mass (Da):53,310
Last modified:October 1, 1996 - v2
Checksum:iBBED6EA47E6F547B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti224 – 2241L → P in CAA28572 (PubMed:3031581).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04884 Genomic DNA. Translation: CAA28572.1.
Z47814 Genomic DNA. Translation: CAA87802.1.
X95966 Genomic DNA. Translation: CAA65216.1.
Z74319 Genomic DNA. Translation: CAA98844.1.
L15408 Genomic DNA. Translation: AAC37412.1.
BK006938 Genomic DNA. Translation: DAA11869.1.
PIRiS50930. YSBYC.
RefSeqiNP_010306.1. NM_001180331.1.

Genome annotation databases

EnsemblFungiiYDR023W; YDR023W; YDR023W.
GeneIDi851587.
KEGGisce:YDR023W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04884 Genomic DNA. Translation: CAA28572.1.
Z47814 Genomic DNA. Translation: CAA87802.1.
X95966 Genomic DNA. Translation: CAA65216.1.
Z74319 Genomic DNA. Translation: CAA98844.1.
L15408 Genomic DNA. Translation: AAC37412.1.
BK006938 Genomic DNA. Translation: DAA11869.1.
PIRiS50930. YSBYC.
RefSeqiNP_010306.1. NM_001180331.1.

3D structure databases

ProteinModelPortaliP07284.
SMRiP07284. Positions 1-451.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32074. 47 interactions.
DIPiDIP-5181N.
IntActiP07284. 3 interactions.
MINTiMINT-513156.
STRINGi4932.YDR023W.

Proteomic databases

MaxQBiP07284.
PaxDbiP07284.
PeptideAtlasiP07284.
PRIDEiP07284.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR023W; YDR023W; YDR023W.
GeneIDi851587.
KEGGisce:YDR023W.

Organism-specific databases

CYGDiYDR023w.
SGDiS000002430. SES1.

Phylogenomic databases

eggNOGiCOG0172.
GeneTreeiENSGT00780000121981.
HOGENOMiHOG000035937.
InParanoidiP07284.
KOiK01875.
OMAiVEAKMEY.
OrthoDBiEOG7PVWZM.

Enzyme and pathway databases

UniPathwayiUPA00906; UER00895.
BioCyciYEAST:G3O-29640-MONOMER.

Miscellaneous databases

NextBioi969063.
PROiP07284.

Gene expression databases

GenevestigatoriP07284.

Family and domain databases

Gene3Di1.10.287.40. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-ligase_type_1.
IPR015866. Ser-tRNA-synth_1_N.
IPR010978. tRNA-bd_arm.
[Graphical view]
PANTHERiPTHR11778. PTHR11778. 1 hit.
PfamiPF02403. Seryl_tRNA_N. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PIRSFiPIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTSiPR00981. TRNASYNTHSER.
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00414. serS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the gene coding for cytoplasmic seryl-tRNA synthetase from Saccharomyces cerevisiae."
    Weygand-Durasevic I., Johnson-Burke D., Soell D.
    Nucleic Acids Res. 15:1887-1904(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT.
  2. "Sequencing and analysis of a 35.4 kb region on the right arm of chromosome IV from Saccharomyces cerevisiae reveal 23 open reading frames."
    Eide L.G., Sander C., Prydz H.
    Yeast 12:1085-1090(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Reduced dosage of genes encoding ribosomal protein S18 suppresses a mitochondrial initiation codon mutation in Saccharomyces cerevisiae."
    Folley L.S., Fox T.D.
    Genetics 137:369-379(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 451-462.
    Strain: ATCC 204508 / S288c.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYSC_YEAST
AccessioniPrimary (citable) accession number: P07284
Secondary accession number(s): D6VS09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: October 1, 1996
Last modified: February 4, 2015
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.