Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine--tRNA ligase, cytoplasmic

Gene

SES1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).1 Publication

Catalytic activityi

ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser).
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).

Pathwayi: selenocysteinyl-tRNA(Sec) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec).
Proteins known to be involved in this subpathway in this organism are:
  1. Serine--tRNA ligase, cytoplasmic (SES1), Serine--tRNA ligase, mitochondrial (DIA4)
This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei295ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei302SerineBy similarity1
Binding sitei404SerineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi279 – 281ATPBy similarity3
Nucleotide bindingi366 – 369ATPBy similarity4

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • serine-tRNA ligase activity Source: SGD

GO - Biological processi

  • selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway
  • seryl-tRNA aminoacylation Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29640-MONOMER
UniPathwayiUPA00906; UER00895

Names & Taxonomyi

Protein namesi
Recommended name:
Serine--tRNA ligase, cytoplasmic (EC:6.1.1.11)
Alternative name(s):
Seryl-tRNA synthetase
Short name:
SerRS
Seryl-tRNA(Ser/Sec) synthetase
Gene namesi
Name:SES1
Synonyms:SERS
Ordered Locus Names:YDR023W
ORF Names:YD9813.01
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR023W
SGDiS000002430 SES1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001221991 – 462Serine--tRNA ligase, cytoplasmicAdd BLAST462

Proteomic databases

MaxQBiP07284
PaxDbiP07284
PRIDEiP07284

PTM databases

CarbonylDBiP07284
iPTMnetiP07284

Interactioni

Subunit structurei

Homodimer. The tRNA molecule probably binds across the dimer.1 Publication

Protein-protein interaction databases

BioGridi32074, 53 interactors
DIPiDIP-5181N
IntActiP07284, 11 interactors
MINTiP07284
STRINGi4932.YDR023W

Structurei

3D structure databases

ProteinModelPortaliP07284
SMRiP07284
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni246 – 248Serine bindingBy similarity3

Domaini

Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.By similarity

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00790000123098
HOGENOMiHOG000035937
InParanoidiP07284
KOiK01875
OMAiSPCFRRE
OrthoDBiEOG092C513S

Family and domain databases

CDDicd00770 SerRS_core, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR002317 Ser-tRNA-ligase_type_1
IPR015866 Ser-tRNA-synth_1_N
IPR033729 SerRS_core
IPR010978 tRNA-bd_arm
PANTHERiPTHR11778 PTHR11778, 1 hit
PfamiView protein in Pfam
PF02403 Seryl_tRNA_N, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PIRSFiPIRSF001529 Ser-tRNA-synth_IIa, 1 hit
PRINTSiPR00981 TRNASYNTHSER
SUPFAMiSSF46589 SSF46589, 1 hit
TIGRFAMsiTIGR00414 serS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

P07284-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDINQFIED KGGNPELIRQ SQKARNASVE IVDEIISDYK DWVKTRFELD
60 70 80 90 100
ELNKKFNKLQ KDIGLKFKNK EDASGLLAEK EKLTQQKKEL TEKEQQEDKD
110 120 130 140 150
LKKKVFQVGN IVHPSVVVSN DEENNELVRT WKPEDLEAVG PIASVTGKPA
160 170 180 190 200
SLSHHEILLR LDGYDPDRGV KICGHRGYFF RNYGVFLNQA LINYGLQFLA
210 220 230 240 250
AKGYIPLQAP VMMNKELMSK TAQLSEFDEE LYKVIDGEDE KYLIATSEQP
260 270 280 290 300
ISAYHSGEWF EKPQEQLPIH YVGYSSCFRR EAGSHGKDAW GVFRVHAFEK
310 320 330 340 350
IEQFVITEPE KSWEEFEKMI SYSEEFYKSL KLPYRIVGIV SGELNNAAAK
360 370 380 390 400
KYDLEAWFPY QKEYKELVSC SNCTDYQSRN LEIRCGIKKM GDREKKYVHC
410 420 430 440 450
LNSTLAATQR ALCCILENYQ TEDGLVVPEV LRKYIPGEPE FLPFVNELPK
460
NSTSSKDKKK KN
Length:462
Mass (Da):53,310
Last modified:October 1, 1996 - v2
Checksum:iBBED6EA47E6F547B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti224L → P in CAA28572 (PubMed:3031581).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04884 Genomic DNA Translation: CAA28572.1
Z47814 Genomic DNA Translation: CAA87802.1
X95966 Genomic DNA Translation: CAA65216.1
Z74319 Genomic DNA Translation: CAA98844.1
L15408 Genomic DNA Translation: AAC37412.1
BK006938 Genomic DNA Translation: DAA11869.1
PIRiS50930 YSBYC
RefSeqiNP_010306.1, NM_001180331.1

Genome annotation databases

EnsemblFungiiYDR023W; YDR023W; YDR023W
GeneIDi851587
KEGGisce:YDR023W

Similar proteinsi

Entry informationi

Entry nameiSYSC_YEAST
AccessioniPrimary (citable) accession number: P07284
Secondary accession number(s): D6VS09
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 173 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health