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P07283 (PMM_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphomannomutase

Short name=PMM
EC=5.4.2.8
Gene names
Name:SEC53
Synonyms:ALG4
Ordered Locus Names:YFL045C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

Catalytic activity

Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.

Pathway

Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Miscellaneous

Present with 3500 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the eukaryotic PMM family.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionIsomerase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGDP-mannose biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

mannose biosynthetic process

Inferred from electronic annotation. Source: InterPro

protein targeting to ER

Inferred from mutant phenotype PubMed 6368572. Source: SGD

   Cellular_componentcytosol

Inferred from direct assay Ref.1. Source: SGD

   Molecular_functionphosphomannomutase activity

Inferred from direct assay Ref.6. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

APM4Q991861EBI-13538,EBI-2715

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 254254Phosphomannomutase
PRO_0000199703

Sites

Active site191Nucleophile By similarity
Active site211Proton donor/acceptor Potential
Binding site281Substrate By similarity
Binding site1301Substrate By similarity
Binding site1411Substrate By similarity
Binding site1481Substrate By similarity
Binding site1861Substrate By similarity
Binding site1881Substrate By similarity

Amino acid modifications

Modified residue2401Phosphoserine Ref.8 Ref.9

Natural variations

Natural variant2341I → T in strain: CLIB 556 haplotype Ha1. Ref.2
Natural variant2491T → I in strain: R12 haplotype Ha1. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P07283 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: B58C88A746368779

FASTA25429,063
        10         20         30         40         50         60 
MSIAEFAYKE KPETLVLFDV DGTLTPARLT VSEEVRKTLA KLRNKCCIGF VGGSDLSKQL 

        70         80         90        100        110        120 
EQLGPNVLDE FDYSFSENGL TAYRLGKELA SQSFINWLGE EKYNKLAVFI LRYLSEIDLP 

       130        140        150        160        170        180 
KRRGTFLEFR NGMINVSPIG RNASTEERNE FERYDKEHQI RAKFVEALKK EFPDYGLTFS 

       190        200        210        220        230        240 
IGGQISFDVF PAGWDKTYCL QHVEKDGFKE IHFFGDKTMV GGNDYEIFVD ERTIGHSVQS 

       250 
PDDTVKILTE LFNL 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of a gene product (Sec53p) required for protein assembly in the yeast endoplasmic reticulum."
Bernstein M., Hoffmann W., Ammerer G., Schekman R.
J. Cell Biol. 101:2374-2382(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Differential evolution of the Saccharomyces cerevisiae DUP240 paralogs and implication of recombination in phylogeny."
Leh-Louis V., Wirth B., Despons L., Wain-Hobson S., Potier S., Souciet J.-L.
Nucleic Acids Res. 32:2069-2078(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-234 AND ILE-249.
Strain: CLIB 219, CLIB 382, CLIB 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, CLIB 95, K1, R12, R13, Sigma 1278B, YIIc12 and YIIc17.
[3]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"The yeast SEC53 gene encodes phosphomannomutase."
Kepes F., Schekman R.
J. Biol. Chem. 263:9155-9161(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-240, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-240, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03213 Genomic DNA. Translation: CAA26957.1.
AJ585720 Genomic DNA. Translation: CAE52240.1.
AJ585721 Genomic DNA. Translation: CAE52241.1.
AJ585722 Genomic DNA. Translation: CAE52242.1.
AJ585723 Genomic DNA. Translation: CAE52243.1.
AJ585724 Genomic DNA. Translation: CAE52244.1.
AJ585725 Genomic DNA. Translation: CAE52245.1.
AJ585726 Genomic DNA. Translation: CAE52246.1.
AJ585727 Genomic DNA. Translation: CAE52247.1.
AJ585728 Genomic DNA. Translation: CAE52248.1.
AJ585729 Genomic DNA. Translation: CAE52249.1.
AJ585730 Genomic DNA. Translation: CAE52250.1.
AJ585731 Genomic DNA. Translation: CAE52251.1.
AJ585732 Genomic DNA. Translation: CAE52252.1.
AJ585733 Genomic DNA. Translation: CAE52253.1.
AJ585734 Genomic DNA. Translation: CAE52254.1.
AJ585735 Genomic DNA. Translation: CAE52255.1.
D50617 Genomic DNA. Translation: BAA09196.1.
AY692959 Genomic DNA. Translation: AAT92978.1.
BK006940 Genomic DNA. Translation: DAA12395.1.
PIRBVBY53. S05874.
RefSeqNP_116609.1. NM_001179922.1.

3D structure databases

ProteinModelPortalP07283.
SMRP07283. Positions 15-245.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31102. 56 interactions.
DIPDIP-4312N.
IntActP07283. 16 interactions.
MINTMINT-526794.

2D gel databases

SWISS-2DPAGEP07283.

Proteomic databases

MaxQBP07283.
PaxDbP07283.
PeptideAtlasP07283.
PRIDEP07283.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFL045C; YFL045C; YFL045C.
GeneID850499.
KEGGsce:YFL045C.

Organism-specific databases

CYGDYFL045c.
SGDS000001849. SEC53.

Phylogenomic databases

eggNOGCOG0561.
GeneTreeENSGT00390000002918.
HOGENOMHOG000181843.
KOK17497.
OMATYCLQHV.
OrthoDBEOG7X3R31.

Enzyme and pathway databases

BioCycYEAST:YFL045C-MONOMER.
UniPathwayUPA00126; UER00424.

Gene expression databases

GenevestigatorP07283.

Family and domain databases

Gene3D3.40.50.1000. 2 hits.
InterProIPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR005002. PMM.
[Graphical view]
PANTHERPTHR10466. PTHR10466. 1 hit.
PfamPF03332. PMM. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR01484. HAD-SF-IIB. 1 hit.
ProtoNetSearch...

Other

NextBio966191.

Entry information

Entry namePMM_YEAST
AccessionPrimary (citable) accession number: P07283
Secondary accession number(s): D6VTI5, Q70D76, Q70D77
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: May 14, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways