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P07283

- PMM_YEAST

UniProt

P07283 - PMM_YEAST

Protein

Phosphomannomutase

Gene

SEC53

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

    Catalytic activityi

    Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei19 – 191NucleophileBy similarity
    Active sitei21 – 211Proton donor/acceptorSequence Analysis
    Binding sitei28 – 281SubstrateBy similarity
    Binding sitei130 – 1301SubstrateBy similarity
    Binding sitei141 – 1411SubstrateBy similarity
    Binding sitei148 – 1481SubstrateBy similarity
    Binding sitei186 – 1861SubstrateBy similarity
    Binding sitei188 – 1881SubstrateBy similarity

    GO - Molecular functioni

    1. phosphomannomutase activity Source: SGD

    GO - Biological processi

    1. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
    2. mannose biosynthetic process Source: InterPro
    3. protein targeting to ER Source: SGD

    Keywords - Molecular functioni

    Isomerase

    Enzyme and pathway databases

    BioCyciYEAST:YFL045C-MONOMER.
    ReactomeiREACT_189212. Synthesis of GDP-mannose.
    UniPathwayiUPA00126; UER00424.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphomannomutase (EC:5.4.2.8)
    Short name:
    PMM
    Gene namesi
    Name:SEC53
    Synonyms:ALG4
    Ordered Locus Names:YFL045C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFL045c.
    SGDiS000001849. SEC53.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 254254PhosphomannomutasePRO_0000199703Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei240 – 2401Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP07283.
    PaxDbiP07283.
    PeptideAtlasiP07283.
    PRIDEiP07283.

    2D gel databases

    SWISS-2DPAGEP07283.

    Expressioni

    Gene expression databases

    GenevestigatoriP07283.

    Interactioni

    Subunit structurei

    Homodimer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    APM4Q991861EBI-13538,EBI-2715

    Protein-protein interaction databases

    BioGridi31102. 56 interactions.
    DIPiDIP-4312N.
    IntActiP07283. 16 interactions.
    MINTiMINT-526794.

    Structurei

    3D structure databases

    ProteinModelPortaliP07283.
    SMRiP07283. Positions 15-245.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the eukaryotic PMM family.Curated

    Phylogenomic databases

    eggNOGiCOG0561.
    GeneTreeiENSGT00390000002918.
    HOGENOMiHOG000181843.
    KOiK17497.
    OMAiTYCLQHV.
    OrthoDBiEOG7X3R31.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    InterProiIPR023214. HAD-like_dom.
    IPR006379. HAD-SF_hydro_IIB.
    IPR005002. PMM.
    [Graphical view]
    PANTHERiPTHR10466. PTHR10466. 1 hit.
    PfamiPF03332. PMM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P07283-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSIAEFAYKE KPETLVLFDV DGTLTPARLT VSEEVRKTLA KLRNKCCIGF    50
    VGGSDLSKQL EQLGPNVLDE FDYSFSENGL TAYRLGKELA SQSFINWLGE 100
    EKYNKLAVFI LRYLSEIDLP KRRGTFLEFR NGMINVSPIG RNASTEERNE 150
    FERYDKEHQI RAKFVEALKK EFPDYGLTFS IGGQISFDVF PAGWDKTYCL 200
    QHVEKDGFKE IHFFGDKTMV GGNDYEIFVD ERTIGHSVQS PDDTVKILTE 250
    LFNL 254
    Length:254
    Mass (Da):29,063
    Last modified:April 1, 1988 - v1
    Checksum:iB58C88A746368779
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti234 – 2341I → T in strain: CLIB 556 haplotype Ha1. 1 Publication
    Natural varianti249 – 2491T → I in strain: R12 haplotype Ha1. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03213 Genomic DNA. Translation: CAA26957.1.
    AJ585720 Genomic DNA. Translation: CAE52240.1.
    AJ585721 Genomic DNA. Translation: CAE52241.1.
    AJ585722 Genomic DNA. Translation: CAE52242.1.
    AJ585723 Genomic DNA. Translation: CAE52243.1.
    AJ585724 Genomic DNA. Translation: CAE52244.1.
    AJ585725 Genomic DNA. Translation: CAE52245.1.
    AJ585726 Genomic DNA. Translation: CAE52246.1.
    AJ585727 Genomic DNA. Translation: CAE52247.1.
    AJ585728 Genomic DNA. Translation: CAE52248.1.
    AJ585729 Genomic DNA. Translation: CAE52249.1.
    AJ585730 Genomic DNA. Translation: CAE52250.1.
    AJ585731 Genomic DNA. Translation: CAE52251.1.
    AJ585732 Genomic DNA. Translation: CAE52252.1.
    AJ585733 Genomic DNA. Translation: CAE52253.1.
    AJ585734 Genomic DNA. Translation: CAE52254.1.
    AJ585735 Genomic DNA. Translation: CAE52255.1.
    D50617 Genomic DNA. Translation: BAA09196.1.
    AY692959 Genomic DNA. Translation: AAT92978.1.
    BK006940 Genomic DNA. Translation: DAA12395.1.
    PIRiS05874. BVBY53.
    RefSeqiNP_116609.1. NM_001179922.1.

    Genome annotation databases

    EnsemblFungiiYFL045C; YFL045C; YFL045C.
    GeneIDi850499.
    KEGGisce:YFL045C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03213 Genomic DNA. Translation: CAA26957.1 .
    AJ585720 Genomic DNA. Translation: CAE52240.1 .
    AJ585721 Genomic DNA. Translation: CAE52241.1 .
    AJ585722 Genomic DNA. Translation: CAE52242.1 .
    AJ585723 Genomic DNA. Translation: CAE52243.1 .
    AJ585724 Genomic DNA. Translation: CAE52244.1 .
    AJ585725 Genomic DNA. Translation: CAE52245.1 .
    AJ585726 Genomic DNA. Translation: CAE52246.1 .
    AJ585727 Genomic DNA. Translation: CAE52247.1 .
    AJ585728 Genomic DNA. Translation: CAE52248.1 .
    AJ585729 Genomic DNA. Translation: CAE52249.1 .
    AJ585730 Genomic DNA. Translation: CAE52250.1 .
    AJ585731 Genomic DNA. Translation: CAE52251.1 .
    AJ585732 Genomic DNA. Translation: CAE52252.1 .
    AJ585733 Genomic DNA. Translation: CAE52253.1 .
    AJ585734 Genomic DNA. Translation: CAE52254.1 .
    AJ585735 Genomic DNA. Translation: CAE52255.1 .
    D50617 Genomic DNA. Translation: BAA09196.1 .
    AY692959 Genomic DNA. Translation: AAT92978.1 .
    BK006940 Genomic DNA. Translation: DAA12395.1 .
    PIRi S05874. BVBY53.
    RefSeqi NP_116609.1. NM_001179922.1.

    3D structure databases

    ProteinModelPortali P07283.
    SMRi P07283. Positions 15-245.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31102. 56 interactions.
    DIPi DIP-4312N.
    IntActi P07283. 16 interactions.
    MINTi MINT-526794.

    2D gel databases

    SWISS-2DPAGE P07283.

    Proteomic databases

    MaxQBi P07283.
    PaxDbi P07283.
    PeptideAtlasi P07283.
    PRIDEi P07283.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFL045C ; YFL045C ; YFL045C .
    GeneIDi 850499.
    KEGGi sce:YFL045C.

    Organism-specific databases

    CYGDi YFL045c.
    SGDi S000001849. SEC53.

    Phylogenomic databases

    eggNOGi COG0561.
    GeneTreei ENSGT00390000002918.
    HOGENOMi HOG000181843.
    KOi K17497.
    OMAi TYCLQHV.
    OrthoDBi EOG7X3R31.

    Enzyme and pathway databases

    UniPathwayi UPA00126 ; UER00424 .
    BioCyci YEAST:YFL045C-MONOMER.
    Reactomei REACT_189212. Synthesis of GDP-mannose.

    Miscellaneous databases

    NextBioi 966191.

    Gene expression databases

    Genevestigatori P07283.

    Family and domain databases

    Gene3Di 3.40.50.1000. 2 hits.
    InterProi IPR023214. HAD-like_dom.
    IPR006379. HAD-SF_hydro_IIB.
    IPR005002. PMM.
    [Graphical view ]
    PANTHERi PTHR10466. PTHR10466. 1 hit.
    Pfami PF03332. PMM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 1 hit.
    TIGRFAMsi TIGR01484. HAD-SF-IIB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a gene product (Sec53p) required for protein assembly in the yeast endoplasmic reticulum."
      Bernstein M., Hoffmann W., Ammerer G., Schekman R.
      J. Cell Biol. 101:2374-2382(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Differential evolution of the Saccharomyces cerevisiae DUP240 paralogs and implication of recombination in phylogeny."
      Leh-Louis V., Wirth B., Despons L., Wain-Hobson S., Potier S., Souciet J.-L.
      Nucleic Acids Res. 32:2069-2078(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-234 AND ILE-249.
      Strain: CLIB 219, CLIB 382, CLIB 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, CLIB 95, K1, R12, R13, Sigma 1278B, YIIc12 and YIIc17.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "The yeast SEC53 gene encodes phosphomannomutase."
      Kepes F., Schekman R.
      J. Biol. Chem. 263:9155-9161(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-240, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-240, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPMM_YEAST
    AccessioniPrimary (citable) accession number: P07283
    Secondary accession number(s): D6VTI5, Q70D76, Q70D77
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3500 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3