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P07283

- PMM_YEAST

UniProt

P07283 - PMM_YEAST

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Protein
Phosphomannomutase
Gene
SEC53, ALG4, YFL045C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

Catalytic activityi

Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei19 – 191Nucleophile By similarity
Active sitei21 – 211Proton donor/acceptor Reviewed prediction
Binding sitei28 – 281Substrate By similarity
Binding sitei130 – 1301Substrate By similarity
Binding sitei141 – 1411Substrate By similarity
Binding sitei148 – 1481Substrate By similarity
Binding sitei186 – 1861Substrate By similarity
Binding sitei188 – 1881Substrate By similarity

GO - Molecular functioni

  1. phosphomannomutase activity Source: SGD

GO - Biological processi

  1. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
  2. mannose biosynthetic process Source: InterPro
  3. protein targeting to ER Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciYEAST:YFL045C-MONOMER.
ReactomeiREACT_189212. Synthesis of GDP-mannose.
UniPathwayiUPA00126; UER00424.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphomannomutase (EC:5.4.2.8)
Short name:
PMM
Gene namesi
Name:SEC53
Synonyms:ALG4
Ordered Locus Names:YFL045C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFL045c.
SGDiS000001849. SEC53.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 254254Phosphomannomutase
PRO_0000199703Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei240 – 2401Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07283.
PaxDbiP07283.
PeptideAtlasiP07283.
PRIDEiP07283.

2D gel databases

SWISS-2DPAGEP07283.

Expressioni

Gene expression databases

GenevestigatoriP07283.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
APM4Q991861EBI-13538,EBI-2715

Protein-protein interaction databases

BioGridi31102. 56 interactions.
DIPiDIP-4312N.
IntActiP07283. 16 interactions.
MINTiMINT-526794.

Structurei

3D structure databases

ProteinModelPortaliP07283.
SMRiP07283. Positions 15-245.

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic PMM family.

Phylogenomic databases

eggNOGiCOG0561.
GeneTreeiENSGT00390000002918.
HOGENOMiHOG000181843.
KOiK17497.
OMAiTYCLQHV.
OrthoDBiEOG7X3R31.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR005002. PMM.
[Graphical view]
PANTHERiPTHR10466. PTHR10466. 1 hit.
PfamiPF03332. PMM. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.

Sequencei

Sequence statusi: Complete.

P07283-1 [UniParc]FASTAAdd to Basket

« Hide

MSIAEFAYKE KPETLVLFDV DGTLTPARLT VSEEVRKTLA KLRNKCCIGF    50
VGGSDLSKQL EQLGPNVLDE FDYSFSENGL TAYRLGKELA SQSFINWLGE 100
EKYNKLAVFI LRYLSEIDLP KRRGTFLEFR NGMINVSPIG RNASTEERNE 150
FERYDKEHQI RAKFVEALKK EFPDYGLTFS IGGQISFDVF PAGWDKTYCL 200
QHVEKDGFKE IHFFGDKTMV GGNDYEIFVD ERTIGHSVQS PDDTVKILTE 250
LFNL 254
Length:254
Mass (Da):29,063
Last modified:April 1, 1988 - v1
Checksum:iB58C88A746368779
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti234 – 2341I → T in strain: CLIB 556 haplotype Ha1. 1 Publication
Natural varianti249 – 2491T → I in strain: R12 haplotype Ha1. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03213 Genomic DNA. Translation: CAA26957.1.
AJ585720 Genomic DNA. Translation: CAE52240.1.
AJ585721 Genomic DNA. Translation: CAE52241.1.
AJ585722 Genomic DNA. Translation: CAE52242.1.
AJ585723 Genomic DNA. Translation: CAE52243.1.
AJ585724 Genomic DNA. Translation: CAE52244.1.
AJ585725 Genomic DNA. Translation: CAE52245.1.
AJ585726 Genomic DNA. Translation: CAE52246.1.
AJ585727 Genomic DNA. Translation: CAE52247.1.
AJ585728 Genomic DNA. Translation: CAE52248.1.
AJ585729 Genomic DNA. Translation: CAE52249.1.
AJ585730 Genomic DNA. Translation: CAE52250.1.
AJ585731 Genomic DNA. Translation: CAE52251.1.
AJ585732 Genomic DNA. Translation: CAE52252.1.
AJ585733 Genomic DNA. Translation: CAE52253.1.
AJ585734 Genomic DNA. Translation: CAE52254.1.
AJ585735 Genomic DNA. Translation: CAE52255.1.
D50617 Genomic DNA. Translation: BAA09196.1.
AY692959 Genomic DNA. Translation: AAT92978.1.
BK006940 Genomic DNA. Translation: DAA12395.1.
PIRiS05874. BVBY53.
RefSeqiNP_116609.1. NM_001179922.1.

Genome annotation databases

EnsemblFungiiYFL045C; YFL045C; YFL045C.
GeneIDi850499.
KEGGisce:YFL045C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03213 Genomic DNA. Translation: CAA26957.1 .
AJ585720 Genomic DNA. Translation: CAE52240.1 .
AJ585721 Genomic DNA. Translation: CAE52241.1 .
AJ585722 Genomic DNA. Translation: CAE52242.1 .
AJ585723 Genomic DNA. Translation: CAE52243.1 .
AJ585724 Genomic DNA. Translation: CAE52244.1 .
AJ585725 Genomic DNA. Translation: CAE52245.1 .
AJ585726 Genomic DNA. Translation: CAE52246.1 .
AJ585727 Genomic DNA. Translation: CAE52247.1 .
AJ585728 Genomic DNA. Translation: CAE52248.1 .
AJ585729 Genomic DNA. Translation: CAE52249.1 .
AJ585730 Genomic DNA. Translation: CAE52250.1 .
AJ585731 Genomic DNA. Translation: CAE52251.1 .
AJ585732 Genomic DNA. Translation: CAE52252.1 .
AJ585733 Genomic DNA. Translation: CAE52253.1 .
AJ585734 Genomic DNA. Translation: CAE52254.1 .
AJ585735 Genomic DNA. Translation: CAE52255.1 .
D50617 Genomic DNA. Translation: BAA09196.1 .
AY692959 Genomic DNA. Translation: AAT92978.1 .
BK006940 Genomic DNA. Translation: DAA12395.1 .
PIRi S05874. BVBY53.
RefSeqi NP_116609.1. NM_001179922.1.

3D structure databases

ProteinModelPortali P07283.
SMRi P07283. Positions 15-245.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31102. 56 interactions.
DIPi DIP-4312N.
IntActi P07283. 16 interactions.
MINTi MINT-526794.

2D gel databases

SWISS-2DPAGE P07283.

Proteomic databases

MaxQBi P07283.
PaxDbi P07283.
PeptideAtlasi P07283.
PRIDEi P07283.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFL045C ; YFL045C ; YFL045C .
GeneIDi 850499.
KEGGi sce:YFL045C.

Organism-specific databases

CYGDi YFL045c.
SGDi S000001849. SEC53.

Phylogenomic databases

eggNOGi COG0561.
GeneTreei ENSGT00390000002918.
HOGENOMi HOG000181843.
KOi K17497.
OMAi TYCLQHV.
OrthoDBi EOG7X3R31.

Enzyme and pathway databases

UniPathwayi UPA00126 ; UER00424 .
BioCyci YEAST:YFL045C-MONOMER.
Reactomei REACT_189212. Synthesis of GDP-mannose.

Miscellaneous databases

NextBioi 966191.

Gene expression databases

Genevestigatori P07283.

Family and domain databases

Gene3Di 3.40.50.1000. 2 hits.
InterProi IPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR005002. PMM.
[Graphical view ]
PANTHERi PTHR10466. PTHR10466. 1 hit.
Pfami PF03332. PMM. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01484. HAD-SF-IIB. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a gene product (Sec53p) required for protein assembly in the yeast endoplasmic reticulum."
    Bernstein M., Hoffmann W., Ammerer G., Schekman R.
    J. Cell Biol. 101:2374-2382(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Differential evolution of the Saccharomyces cerevisiae DUP240 paralogs and implication of recombination in phylogeny."
    Leh-Louis V., Wirth B., Despons L., Wain-Hobson S., Potier S., Souciet J.-L.
    Nucleic Acids Res. 32:2069-2078(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-234 AND ILE-249.
    Strain: CLIB 219, CLIB 382, CLIB 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, CLIB 95, K1, R12, R13, Sigma 1278B, YIIc12 and YIIc17.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "The yeast SEC53 gene encodes phosphomannomutase."
    Kepes F., Schekman R.
    J. Biol. Chem. 263:9155-9161(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-240, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-240, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPMM_YEAST
AccessioniPrimary (citable) accession number: P07283
Secondary accession number(s): D6VTI5, Q70D76, Q70D77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: September 3, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3500 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

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