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Protein

Phosphomannomutase

Gene

SEC53

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

Miscellaneous

Present with 3500 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.

Pathwayi: GDP-alpha-D-mannose biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Mannose-6-phosphate isomerase (PMI40)
  2. Phosphomannomutase (SEC53)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate, the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei19NucleophileBy similarity1
Active sitei21Proton donor/acceptorSequence analysis1
Binding sitei28SubstrateBy similarity1
Binding sitei130SubstrateBy similarity1
Binding sitei141SubstrateBy similarity1
Binding sitei148SubstrateBy similarity1
Binding sitei186SubstrateBy similarity1
Binding sitei188SubstrateBy similarity1

GO - Molecular functioni

  • phosphomannomutase activity Source: SGD

GO - Biological processi

  • GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
  • mannose metabolic process Source: GO_Central
  • protein N-linked glycosylation Source: GO_Central
  • protein targeting to ER Source: SGD

Keywordsi

Molecular functionIsomerase

Enzyme and pathway databases

BioCyciYEAST:YFL045C-MONOMER
ReactomeiR-SCE-446205 Synthesis of GDP-mannose
UniPathwayiUPA00126; UER00424

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphomannomutase (EC:5.4.2.8)
Short name:
PMM
Gene namesi
Name:SEC53
Synonyms:ALG4
Ordered Locus Names:YFL045C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL045C
SGDiS000001849 SEC53

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001997031 – 254PhosphomannomutaseAdd BLAST254

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei240PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07283
PaxDbiP07283
PRIDEiP07283

2D gel databases

SWISS-2DPAGEiP07283

PTM databases

iPTMnetiP07283

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi31102, 231 interactors
DIPiDIP-4312N
IntActiP07283, 28 interactors
MINTiP07283
STRINGi4932.YFL045C

Structurei

3D structure databases

ProteinModelPortaliP07283
SMRiP07283
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic PMM family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000002918
HOGENOMiHOG000181843
InParanoidiP07283
KOiK17497
OMAiTYCLQHL
OrthoDBiEOG092C4IV8

Family and domain databases

CDDicd02585 HAD_PMM, 1 hit
Gene3Di3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR036412 HAD-like_sf
IPR006379 HAD-SF_hydro_IIB
IPR023214 HAD_sf
IPR005002 PMM
PANTHERiPTHR10466 PTHR10466, 1 hit
PfamiView protein in Pfam
PF03332 PMM, 1 hit
SFLDiSFLDG01143 C2.B.3:_Phosphomannomutase_Lik, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01484 HAD-SF-IIB, 1 hit

Sequencei

Sequence statusi: Complete.

P07283-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIAEFAYKE KPETLVLFDV DGTLTPARLT VSEEVRKTLA KLRNKCCIGF
60 70 80 90 100
VGGSDLSKQL EQLGPNVLDE FDYSFSENGL TAYRLGKELA SQSFINWLGE
110 120 130 140 150
EKYNKLAVFI LRYLSEIDLP KRRGTFLEFR NGMINVSPIG RNASTEERNE
160 170 180 190 200
FERYDKEHQI RAKFVEALKK EFPDYGLTFS IGGQISFDVF PAGWDKTYCL
210 220 230 240 250
QHVEKDGFKE IHFFGDKTMV GGNDYEIFVD ERTIGHSVQS PDDTVKILTE

LFNL
Length:254
Mass (Da):29,063
Last modified:April 1, 1988 - v1
Checksum:iB58C88A746368779
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti234I → T in strain: CLIB 556 haplotype Ha1. 1 Publication1
Natural varianti249T → I in strain: R12 haplotype Ha1. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03213 Genomic DNA Translation: CAA26957.1
AJ585720 Genomic DNA Translation: CAE52240.1
AJ585721 Genomic DNA Translation: CAE52241.1
AJ585722 Genomic DNA Translation: CAE52242.1
AJ585723 Genomic DNA Translation: CAE52243.1
AJ585724 Genomic DNA Translation: CAE52244.1
AJ585725 Genomic DNA Translation: CAE52245.1
AJ585726 Genomic DNA Translation: CAE52246.1
AJ585727 Genomic DNA Translation: CAE52247.1
AJ585728 Genomic DNA Translation: CAE52248.1
AJ585729 Genomic DNA Translation: CAE52249.1
AJ585730 Genomic DNA Translation: CAE52250.1
AJ585731 Genomic DNA Translation: CAE52251.1
AJ585732 Genomic DNA Translation: CAE52252.1
AJ585733 Genomic DNA Translation: CAE52253.1
AJ585734 Genomic DNA Translation: CAE52254.1
AJ585735 Genomic DNA Translation: CAE52255.1
D50617 Genomic DNA Translation: BAA09196.1
AY692959 Genomic DNA Translation: AAT92978.1
BK006940 Genomic DNA Translation: DAA12395.1
PIRiS05874 BVBY53
RefSeqiNP_116609.1, NM_001179922.1

Genome annotation databases

EnsemblFungiiBAA09196; BAA09196; BAA09196
YFL045C; YFL045C; YFL045C
GeneIDi850499
KEGGisce:YFL045C

Similar proteinsi

Entry informationi

Entry nameiPMM_YEAST
AccessioniPrimary (citable) accession number: P07283
Secondary accession number(s): D6VTI5, Q70D76, Q70D77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: May 23, 2018
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

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