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Protein

Phosphomannomutase

Gene

SEC53

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

Catalytic activityi

Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.

Pathway:iGDP-alpha-D-mannose biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Mannose-6-phosphate isomerase (PMI40)
  2. Phosphomannomutase (SEC53)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate, the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei19 – 191NucleophileBy similarity
Active sitei21 – 211Proton donor/acceptorSequence Analysis
Binding sitei28 – 281SubstrateBy similarity
Binding sitei130 – 1301SubstrateBy similarity
Binding sitei141 – 1411SubstrateBy similarity
Binding sitei148 – 1481SubstrateBy similarity
Binding sitei186 – 1861SubstrateBy similarity
Binding sitei188 – 1881SubstrateBy similarity

GO - Molecular functioni

  • phosphomannomutase activity Source: SGD

GO - Biological processi

  • GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
  • mannose biosynthetic process Source: InterPro
  • protein targeting to ER Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciYEAST:YFL045C-MONOMER.
ReactomeiREACT_328451. Synthesis of GDP-mannose.
UniPathwayiUPA00126; UER00424.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphomannomutase (EC:5.4.2.8)
Short name:
PMM
Gene namesi
Name:SEC53
Synonyms:ALG4
Ordered Locus Names:YFL045C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VI

Organism-specific databases

CYGDiYFL045c.
EuPathDBiFungiDB:YFL045C.
SGDiS000001849. SEC53.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 254254PhosphomannomutasePRO_0000199703Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei240 – 2401Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07283.
PaxDbiP07283.
PeptideAtlasiP07283.
PRIDEiP07283.

2D gel databases

SWISS-2DPAGEP07283.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi31102. 57 interactions.
DIPiDIP-4312N.
IntActiP07283. 16 interactions.
MINTiMINT-526794.

Structurei

3D structure databases

ProteinModelPortaliP07283.
SMRiP07283. Positions 15-245.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic PMM family.Curated

Phylogenomic databases

eggNOGiCOG0561.
GeneTreeiENSGT00390000002918.
HOGENOMiHOG000181843.
InParanoidiP07283.
KOiK17497.
OMAiSIXGGND.
OrthoDBiEOG7X3R31.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR005002. PMM.
[Graphical view]
PANTHERiPTHR10466. PTHR10466. 1 hit.
PfamiPF03332. PMM. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.

Sequencei

Sequence statusi: Complete.

P07283-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIAEFAYKE KPETLVLFDV DGTLTPARLT VSEEVRKTLA KLRNKCCIGF
60 70 80 90 100
VGGSDLSKQL EQLGPNVLDE FDYSFSENGL TAYRLGKELA SQSFINWLGE
110 120 130 140 150
EKYNKLAVFI LRYLSEIDLP KRRGTFLEFR NGMINVSPIG RNASTEERNE
160 170 180 190 200
FERYDKEHQI RAKFVEALKK EFPDYGLTFS IGGQISFDVF PAGWDKTYCL
210 220 230 240 250
QHVEKDGFKE IHFFGDKTMV GGNDYEIFVD ERTIGHSVQS PDDTVKILTE

LFNL
Length:254
Mass (Da):29,063
Last modified:April 1, 1988 - v1
Checksum:iB58C88A746368779
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti234 – 2341I → T in strain: CLIB 556 haplotype Ha1. 1 Publication
Natural varianti249 – 2491T → I in strain: R12 haplotype Ha1. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03213 Genomic DNA. Translation: CAA26957.1.
AJ585720 Genomic DNA. Translation: CAE52240.1.
AJ585721 Genomic DNA. Translation: CAE52241.1.
AJ585722 Genomic DNA. Translation: CAE52242.1.
AJ585723 Genomic DNA. Translation: CAE52243.1.
AJ585724 Genomic DNA. Translation: CAE52244.1.
AJ585725 Genomic DNA. Translation: CAE52245.1.
AJ585726 Genomic DNA. Translation: CAE52246.1.
AJ585727 Genomic DNA. Translation: CAE52247.1.
AJ585728 Genomic DNA. Translation: CAE52248.1.
AJ585729 Genomic DNA. Translation: CAE52249.1.
AJ585730 Genomic DNA. Translation: CAE52250.1.
AJ585731 Genomic DNA. Translation: CAE52251.1.
AJ585732 Genomic DNA. Translation: CAE52252.1.
AJ585733 Genomic DNA. Translation: CAE52253.1.
AJ585734 Genomic DNA. Translation: CAE52254.1.
AJ585735 Genomic DNA. Translation: CAE52255.1.
D50617 Genomic DNA. Translation: BAA09196.1.
AY692959 Genomic DNA. Translation: AAT92978.1.
BK006940 Genomic DNA. Translation: DAA12395.1.
PIRiS05874. BVBY53.
RefSeqiNP_116609.1. NM_001179922.1.

Genome annotation databases

EnsemblFungiiYFL045C; YFL045C; YFL045C.
GeneIDi850499.
KEGGisce:YFL045C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03213 Genomic DNA. Translation: CAA26957.1.
AJ585720 Genomic DNA. Translation: CAE52240.1.
AJ585721 Genomic DNA. Translation: CAE52241.1.
AJ585722 Genomic DNA. Translation: CAE52242.1.
AJ585723 Genomic DNA. Translation: CAE52243.1.
AJ585724 Genomic DNA. Translation: CAE52244.1.
AJ585725 Genomic DNA. Translation: CAE52245.1.
AJ585726 Genomic DNA. Translation: CAE52246.1.
AJ585727 Genomic DNA. Translation: CAE52247.1.
AJ585728 Genomic DNA. Translation: CAE52248.1.
AJ585729 Genomic DNA. Translation: CAE52249.1.
AJ585730 Genomic DNA. Translation: CAE52250.1.
AJ585731 Genomic DNA. Translation: CAE52251.1.
AJ585732 Genomic DNA. Translation: CAE52252.1.
AJ585733 Genomic DNA. Translation: CAE52253.1.
AJ585734 Genomic DNA. Translation: CAE52254.1.
AJ585735 Genomic DNA. Translation: CAE52255.1.
D50617 Genomic DNA. Translation: BAA09196.1.
AY692959 Genomic DNA. Translation: AAT92978.1.
BK006940 Genomic DNA. Translation: DAA12395.1.
PIRiS05874. BVBY53.
RefSeqiNP_116609.1. NM_001179922.1.

3D structure databases

ProteinModelPortaliP07283.
SMRiP07283. Positions 15-245.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31102. 57 interactions.
DIPiDIP-4312N.
IntActiP07283. 16 interactions.
MINTiMINT-526794.

2D gel databases

SWISS-2DPAGEP07283.

Proteomic databases

MaxQBiP07283.
PaxDbiP07283.
PeptideAtlasiP07283.
PRIDEiP07283.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYFL045C; YFL045C; YFL045C.
GeneIDi850499.
KEGGisce:YFL045C.

Organism-specific databases

CYGDiYFL045c.
EuPathDBiFungiDB:YFL045C.
SGDiS000001849. SEC53.

Phylogenomic databases

eggNOGiCOG0561.
GeneTreeiENSGT00390000002918.
HOGENOMiHOG000181843.
InParanoidiP07283.
KOiK17497.
OMAiSIXGGND.
OrthoDBiEOG7X3R31.

Enzyme and pathway databases

UniPathwayiUPA00126; UER00424.
BioCyciYEAST:YFL045C-MONOMER.
ReactomeiREACT_328451. Synthesis of GDP-mannose.

Miscellaneous databases

NextBioi966191.
PROiP07283.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR005002. PMM.
[Graphical view]
PANTHERiPTHR10466. PTHR10466. 1 hit.
PfamiPF03332. PMM. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a gene product (Sec53p) required for protein assembly in the yeast endoplasmic reticulum."
    Bernstein M., Hoffmann W., Ammerer G., Schekman R.
    J. Cell Biol. 101:2374-2382(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Differential evolution of the Saccharomyces cerevisiae DUP240 paralogs and implication of recombination in phylogeny."
    Leh-Louis V., Wirth B., Despons L., Wain-Hobson S., Potier S., Souciet J.-L.
    Nucleic Acids Res. 32:2069-2078(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-234 AND ILE-249.
    Strain: CLIB 219, CLIB 382, CLIB 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, CLIB 95, K1, R12, R13, Sigma 1278B, YIIc12 and YIIc17.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "The yeast SEC53 gene encodes phosphomannomutase."
    Kepes F., Schekman R.
    J. Biol. Chem. 263:9155-9161(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-240, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-240, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPMM_YEAST
AccessioniPrimary (citable) accession number: P07283
Secondary accession number(s): D6VTI5, Q70D76, Q70D77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: July 22, 2015
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.