Phosphomannomutase - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P07283 (PMM_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 128. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phosphomannomutase
Short name=PMM
EC=5.4.2.8

Gene names

Name:	SEC53
Synonyms:	ALG4
Ordered Locus Names:	YFL045C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.
Catalytic activity	Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 3500 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the eukaryotic PMM family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Isomerase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GDP-mannose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway mannose biosynthetic process Inferred from electronic annotation. Source: InterPro protein targeting to ER Inferred from mutant phenotype PubMed 6368572. Source: SGD
Cellular_component	cytosol Inferred from direct assay Ref.1. Source: SGD
Molecular_function	phosphomannomutase activity Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APM4	Q99186	1	EBI-13538,EBI-2715

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 254

254

Phosphomannomutase

PRO_0000199703

Sites

Active site

Nucleophile By similarity

Active site

By similarity

Active site

By similarity

Active site

196

By similarity

Binding site

Substrate By similarity

Binding site

130

Substrate By similarity

Binding site

141

Substrate By similarity

Binding site

148

Substrate By similarity

Binding site

186

Substrate By similarity

Binding site

188

Substrate By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.10

Modified residue

Phosphoserine Ref.10

Modified residue

240

Phosphoserine Ref.8 Ref.9 Ref.10

Natural variations

Natural variant

234

I → T in strain: CLIB 556 haplotype Ha1. Ref.2

Natural variant

249

T → I in strain: R12 haplotype Ha1. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P07283 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: B58C88A746368779
Show »

FASTA

254

29,063

        10         20         30         40         50         60 
MSIAEFAYKE KPETLVLFDV DGTLTPARLT VSEEVRKTLA KLRNKCCIGF VGGSDLSKQL 

        70         80         90        100        110        120 
EQLGPNVLDE FDYSFSENGL TAYRLGKELA SQSFINWLGE EKYNKLAVFI LRYLSEIDLP 

       130        140        150        160        170        180 
KRRGTFLEFR NGMINVSPIG RNASTEERNE FERYDKEHQI RAKFVEALKK EFPDYGLTFS 

       190        200        210        220        230        240 
IGGQISFDVF PAGWDKTYCL QHVEKDGFKE IHFFGDKTMV GGNDYEIFVD ERTIGHSVQS 

       250 
PDDTVKILTE LFNL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a gene product (Sec53p) required for protein assembly in the yeast endoplasmic reticulum." Bernstein M., Hoffmann W., Ammerer G., Schekman R. J. Cell Biol. 101:2374-2382(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Differential evolution of the Saccharomyces cerevisiae DUP240 paralogs and implication of recombination in phylogeny." Leh-Louis V., Wirth B., Despons L., Wain-Hobson S., Potier S., Souciet J.-L. Nucleic Acids Res. 32:2069-2078(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-234 AND ILE-249. Strain: CLIB 219, CLIB 382, CLIB 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, CLIB 95, K1, R12, R13, Sigma 1278B, YIIc12 and YIIc17.
[3]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"The yeast SEC53 gene encodes phosphomannomutase." Kepes F., Schekman R. J. Biol. Chem. 263:9155-9161(1988) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-240, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-240, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; SER-54 AND SER-240, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03213 Genomic DNA. Translation: CAA26957.1. AJ585720 Genomic DNA. Translation: CAE52240.1. AJ585721 Genomic DNA. Translation: CAE52241.1. AJ585722 Genomic DNA. Translation: CAE52242.1. AJ585723 Genomic DNA. Translation: CAE52243.1. AJ585724 Genomic DNA. Translation: CAE52244.1. AJ585725 Genomic DNA. Translation: CAE52245.1. AJ585726 Genomic DNA. Translation: CAE52246.1. AJ585727 Genomic DNA. Translation: CAE52247.1. AJ585728 Genomic DNA. Translation: CAE52248.1. AJ585729 Genomic DNA. Translation: CAE52249.1. AJ585730 Genomic DNA. Translation: CAE52250.1. AJ585731 Genomic DNA. Translation: CAE52251.1. AJ585732 Genomic DNA. Translation: CAE52252.1. AJ585733 Genomic DNA. Translation: CAE52253.1. AJ585734 Genomic DNA. Translation: CAE52254.1. AJ585735 Genomic DNA. Translation: CAE52255.1. D50617 Genomic DNA. Translation: BAA09196.1. AY692959 Genomic DNA. Translation: AAT92978.1. BK006940 Genomic DNA. Translation: DAA12395.1.
PIR	BVBY53. S05874.
RefSeq	NP_116609.1. NM_001179922.1.
3D structure databases
ProteinModelPortal	P07283.
SMR	P07283. Positions 15-245.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4312N.
IntAct	P07283. 26 interactions.
MINT	MINT-526794.
2D gel databases
SWISS-2DPAGE	P07283.
Proteomic databases
PaxDb	P07283.
PeptideAtlas	P07283.
PRIDE	P07283.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL045C; YFL045C; YFL045C.
GeneID	850499.
KEGG	sce:YFL045C.
Organism-specific databases
CYGD	YFL045c.
SGD	S000001849. SEC53.
Phylogenomic databases
eggNOG	COG0561.
GeneTree	ENSGT00390000002918.
HOGENOM	HOG000181843.
KO	K01840.
OMA	EFERYDK.
OrthoDB	EOG4VMJQC.
Enzyme and pathway databases
UniPathway	UPA00126; UER00424.
Gene expression databases
Genevestigator	P07283.
GermOnline	YFL045C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.1000. 2 hits.
InterPro	IPR023214. HAD-like_dom. IPR006379. HAD-SF_hydro_IIB. IPR005002. PMM. [Graphical view]
PANTHER	PTHR10466. PTHR10466. 1 hit.
Pfam	PF03332. PMM. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01484. HAD-SF-IIB. 1 hit.
ProtoNet	Search...
Other
NextBio	966191.

Entry information

Entry name

PMM_YEAST

Accession

Primary (citable) accession number: P07283
Secondary accession number(s): D6VTI5, Q70D76, Q70D77

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	April 3, 2013
This is version 128 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents