Reviewed, UniProtKB/Swiss-Prot P07281 (RS19B_YEAST)

Names and origin

Protein names

Recommended name:
    40S ribosomal protein S19-B
Alternative name(s):
    S16a
    RP55B
    YS16B

Gene names

Name:	RPS19B
Synonyms:	RP55B, RPS16AB
Ordered Locus Names:	YNL302C
ORF Names:	N0422

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.5
Subcellular location	Cytoplasm Ref.7.
Miscellaneous	Present with 3440 molecules/cell in log phase SD medium. Ref.8 There are 2 genes for RP55 in yeast.
Sequence similarities	Belongs to the ribosomal protein S19e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	rRNA export from nucleus Inferred from genetic interaction. Source: SGD ribosomal small subunit biogenesis Inferred from genetic interaction. Source: SGD translation Ref.4 Inferred by curator. Source: SGD
Cellular component	cytosolic small ribosomal subunit Ref.4 Inferred from direct assay. Source: SGD
Molecular function	structural constituent of ribosome Ref.4 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4 Ref.6
	Chain	2 – 144	143	40S ribosomal protein S19-B		PRO_0000153836
Amino acid modifications
	Modified residue	20	1	Phosphoserine Ref.10
	Modified residue	60	1	Phosphoserine Ref.9
	Modified residue	117	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P07281-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: A36AA94B855768CB	FASTA	144	15,891
	10 20 30 40 50 60 MAGVSVRDVA AQDFINAYAS FLQRQGKLEV PGYVDIVKTS SGNEMPPQDA EGWFYKRAAS 70 80 90 100 110 120 VARHIYMRKQ VGVGKLNKLY GGAKSRGVRP YKHIDASGSI NRKVLQALEK IGIVEISPKG 130 140 GRRISENGQR DLDRIAAQTL EEDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and organization of two linked ribosomal protein genes in yeast." Molenaar C.M.T., Woudt L.P., Jansen A.E.M., Mager W.H., Planta R.J., Donovan D.M., Pearson N.J. Nucleic Acids Res. 12:7345-7358(1984) [PubMed: 6387623] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed: 8553702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast." Otaka E., Higo K., Osawa S. Biochemistry 21:4545-4550(1982) [PubMed: 6814480] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-50.
[5]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[6]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed: 10601260] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20 AND SER-117, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X01100 Genomic DNA. Translation: CAA25575.1. U23084 Genomic DNA. Translation: AAC49096.1. Z71578 Genomic DNA. Translation: CAA96220.1.
PIR	S60398.
RefSeq	NP_014097.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P07281.
Proteomic databases
PRIDE	P07281.
Genome annotation databases
Ensembl	YNL302C; YNL302C; YNL302C; Saccharomyces cerevisiae. [Genome view]
GeneID	855414.
KEGG	sce:YNL302C.
NMPDR	fig|4932.3.peg.5159.
Organism-specific databases
CYGD	YNL302c.
SGD	S000005246. RPS19B.
Phylogenomic databases
HOGENOM	HBG594417.
OMA	IYLKENV.
OrthoDB	EOG98D2BX.
Gene expression databases
ArrayExpress	P07281.
Genevestigator	P07281.
GermOnline	YNL302C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001266. Ribosomal_S19e. IPR018277. Ribosomal_S19e_CS. [Graphical view]
PANTHER	PTHR11710. Ribosomal_S19E. 1 hit.
Pfam	PF01090. Ribosomal_S19e. 1 hit. [Graphical view]
ProDom	PD003854. Ribosomal_S19e. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00628. RIBOSOMAL_S19E. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979262.

Entry information

Entry name

RS19B_YEAST

Accession

Primary (citable) accession number: P07281

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	December 15, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)