P07280 (RS19A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S19-A

Alternative name(s):
RP55A
S16a
YP45
YS16A

Gene names

Name:	RPS19A
Synonyms:	RP55A, RPS16AA
Ordered Locus Names:	YOL121C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for proper maturation of the small (40S) ribosomal subunit. Binds to 40s pre-ribosomal particles, probably required after association of NOC4 but before association of ENP1, TSR1 and RIO2 with 20/21S pre-rRNA. Ref.9 Ref.10
Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6
Subcellular location	Cytoplasm Ref.7.
Disruption phenotype	Disruption of a single RPS19 gene reduces cell growth; a double disruption is lethal. Depletion experiments show the proteins are required for correct maturation of precursor rRNA to generate the 18S small rRNA. A specific site between the 18S and 5.8S precursors (site A2 in ETS1) is not cleaved in disruption mutants. Partially assembled ribosomes are retained in the nucleolus rather than being exported to the cytoplasm. All effects are exacerbated in the double disruption. Increases association of NOC4 with 20S/21S pre-rRNA, decreases association of ENP1, TSR1 and RIO2 with 20S/21S pre-rRNA. Ref.9 Ref.10
Miscellaneous	Present with 29000 molecules/cell in log phase SD medium. There are 2 genes for S19 in yeast.
Sequence similarities	Belongs to the ribosomal protein S19e family.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Inferred by curator Ref.5. Source: SGD rRNA export from nucleus Inferred from mutant phenotype Ref.9. Source: SGD ribosomal small subunit biogenesis Inferred from mutant phenotype Ref.9. Source: SGD
Cellular_component	cytosolic small ribosomal subunit Inferred from direct assay Ref.5. Source: SGD
Molecular_function	structural constituent of ribosome Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.5

Chain

2 – 144

143

40S ribosomal protein S19-A

PRO_0000153835

Experimental info

Mutagenesis

15

1

I → F: Partial loss of function; decreased 18S rRNA, decreases binding to 20S pre-rRNA complex, slow growth in double RPS19A/RPS19B mutant. Ref.10

Mutagenesis

57

1

R → E or Q: Loss of mature 18S rRNA, protein doesn't bind 20S pre-RNA complex. Lethal in double RPS19A/RPS19B mutant. Ref.10

Mutagenesis

63

1

R → E: Loss of mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. Ref.10

Mutagenesis

65

1

I → P: Lethal in double RPS19A/RPS19B mutant, considerable decrease in 18S rRNA production. Ref.9 Ref.10

Mutagenesis

102

1

R → E: Decreases mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. Ref.10

Mutagenesis

122

1

R → E: Decreases mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. Ref.10

Secondary structure

1

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144

Helix Strand Turn

Details...

Helix

6 – 8

3

Helix

11 – 25

15

Turn

31 – 35

5

Beta strand

51 – 53

3

Helix

54 – 66

13

Beta strand

68 – 70

3

Helix

73 – 80

8

Beta strand

83 – 86

4

Beta strand

89 – 93

5

Helix

98 – 110

13

Beta strand

113 – 115

3

Beta strand

118 – 121

4

Helix

126 – 142

17

Sequences

	Sequence		Length	Mass (Da)
	P07280 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: FE06D94B993B11B2	FASTA	144	15,917
	10 20 30 40 50 60 MPGVSVRDVA AQDFINAYAS FLQRQGKLEV PGYVDIVKTS SGNEMPPQDA EGWFYKRAAS 70 80 90 100 110 120 VARHIYMRKQ VGVGKLNKLY GGAKSRGVRP YKHIDASGSI NRKVLQALEK IGIVEISPKG 130 140 GRRISENGQR DLDRIAAQTL EEDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and organization of two linked ribosomal protein genes in yeast." Molenaar C.M.T., Woudt L.P., Jansen A.E.M., Mager W.H., Planta R.J., Donovan D.M., Pearson N.J. Nucleic Acids Res. 12:7345-7358(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"DNA sequence analysis of a 10 624 bp fragment of the left arm of chromosome XV from Saccharomyces cerevisiae reveals a RNA binding protein, a mitochondrial protein, two ribosomal proteins and two new open reading frames." Lafuente M.J., Gamo F.-J., Gancedo C. Yeast 12:1041-1045(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast." Otaka E., Higo K., Osawa S. Biochemistry 21:4545-4550(1982) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-50.
[6]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Specific role for yeast homologs of the Diamond Blackfan anemia-associated Rps19 protein in ribosome synthesis." Leger-Silvestre I., Caffrey J.M., Dawaliby R., Alvarez-Arias D.A., Gas N., Bertolone S.J., Gleizes P.E., Ellis S.R. J. Biol. Chem. 280:38177-38185(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN RIBOSOME SYNTHESIS, MUTAGENESIS OF ILE-65, DISRUPTION PHENOTYPE.
[10]	"Molecular basis of Diamond-Blackfan anemia: structure and function analysis of RPS19." Gregory L.A., Aguissa-Toure A.H., Pinaud N., Legrand P., Gleizes P.E., Fribourg S. Nucleic Acids Res. 35:5913-5921(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ILE-15; ARG-57; ARG-63; ILE-65; ARG-102 AND ARG-122.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X02635 Genomic DNA. Translation: CAA26482.1.
X95258 Genomic DNA. Translation: CAA64549.1.
Z74863 Genomic DNA. Translation: CAA99140.1.
BK006948 Genomic DNA. Translation: DAA10663.1.

PIR

R3BY9E. S05868.

RefSeq

NP_014520.1. NM_001183375.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IZB	electron microscopy	-	S	1-144	[»]
3O2Z	X-ray	4.00	M	1-144	[»]
3O30	X-ray	4.00	M	1-144	[»]
3U5C	X-ray	3.00	T	1-144	[»]
3U5G	X-ray	3.00	T	1-144	[»]

ProteinModelPortal

P07280.

SMR

P07280. Positions 2-144.

ModBase

Search...

Protein-protein interaction databases

IntAct

P07280. 29 interactions.

STRING

4932.YOL121C.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YOL121C; YOL121C; YOL121C.

GeneID

854028.

KEGG

sce:YOL121C.

Organism-specific databases

SGD

S000005481. RPS19A.

Phylogenomic databases

GeneTree

ENSGT00390000013102.

HOGENOM

HOG000230649.

KO

K02966.

OMA

WAPFVKT.

OrthoDB

EOG490BKG.

Gene expression databases

Genevestigator

P07280.

GermOnline

YOL121C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001266. Ribosomal_S19e.
IPR018277. Ribosomal_S19e_CS.
[Graphical view]

PANTHER

PTHR11710. PTHR11710. 1 hit.

Pfam

PF01090. Ribosomal_S19e. 1 hit.
[Graphical view]

ProDom

PD003854. Ribosomal_S19e. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00628. RIBOSOMAL_S19E. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

975574.

Entry information

Entry name

RS19A_YEAST

Accession

Primary (citable) accession number: P07280
Secondary accession number(s): D6W1U7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program