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Protein

40S ribosomal protein S19-A

Gene

RPS19A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for proper maturation of the small (40S) ribosomal subunit. Binds to 40s pre-ribosomal particles, probably required after association of NOC4 but before association of ENP1, TSR1 and RIO2 with 20/21S pre-rRNA.2 Publications

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
  2. ribosomal small subunit biogenesis Source: SGD
  3. rRNA export from nucleus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Biological processi

Ribosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-33517-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_248935. Ribosomal scanning and start codon recognition.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257608. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_257951. Peptide chain elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S19-A
Alternative name(s):
RP55A
S16a
YP45
YS16A
Gene namesi
Name:RPS19A
Synonyms:RP55A, RPS16AA
Ordered Locus Names:YOL121C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

SGDiS000005481. RPS19A.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Disruption of a single RPS19 gene reduces cell growth; a double disruption is lethal. Depletion experiments show the proteins are required for correct maturation of precursor rRNA to generate the 18S small rRNA. A specific site between the 18S and 5.8S precursors (site A2 in ETS1) is not cleaved in disruption mutants. Partially assembled ribosomes are retained in the nucleolus rather than being exported to the cytoplasm. All effects are exacerbated in the double disruption. Increases association of NOC4 with 20S/21S pre-rRNA, decreases association of ENP1, TSR1 and RIO2 with 20S/21S pre-rRNA.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi15 – 151I → F: Partial loss of function; decreased 18S rRNA, decreases binding to 20S pre-rRNA complex, slow growth in double RPS19A/RPS19B mutant. 1 Publication
Mutagenesisi57 – 571R → E or Q: Loss of mature 18S rRNA, protein doesn't bind 20S pre-RNA complex. Lethal in double RPS19A/RPS19B mutant. 1 Publication
Mutagenesisi63 – 631R → E: Loss of mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. 1 Publication
Mutagenesisi65 – 651I → P: Lethal in double RPS19A/RPS19B mutant, considerable decrease in 18S rRNA production. 2 Publications
Mutagenesisi102 – 1021R → E: Decreases mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. 1 Publication
Mutagenesisi122 – 1221R → E: Decreases mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 14414340S ribosomal protein S19-APRO_0000153835Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP07280.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi34280. 36 interactions.
IntActiP07280. 31 interactions.
MINTiMINT-4502132.
STRINGi4932.YOL121C.

Structurei

Secondary structure

1
144
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 83Combined sources
Helixi11 – 2515Combined sources
Beta strandi34 – 363Combined sources
Beta strandi39 – 424Combined sources
Beta strandi51 – 533Combined sources
Helixi54 – 6613Combined sources
Beta strandi68 – 714Combined sources
Helixi73 – 808Combined sources
Beta strandi83 – 864Combined sources
Beta strandi89 – 935Combined sources
Helixi98 – 11013Combined sources
Beta strandi113 – 1153Combined sources
Beta strandi120 – 1245Combined sources
Helixi126 – 14116Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10U1-144[»]
3J6Yelectron microscopy6.10U1-144[»]
3J77electron microscopy6.20T1-144[»]
3J78electron microscopy6.30T1-144[»]
3V88X-ray3.00T1-144[»]
4U3MX-ray3.00U/x2-144[»]
4U3NX-ray3.20U/x2-144[»]
4U3UX-ray2.90U/x2-144[»]
4U4NX-ray3.10U/x2-144[»]
4U4OX-ray3.60U/x2-144[»]
4U4QX-ray3.00U/x2-144[»]
4U4RX-ray2.80U/x2-144[»]
4U4UX-ray3.00U/x2-144[»]
4U4YX-ray3.20U/x2-144[»]
4U4ZX-ray3.10U/x2-144[»]
4U50X-ray3.20U/x2-144[»]
4U51X-ray3.20U/x2-144[»]
4U52X-ray3.00U/x2-144[»]
4U53X-ray3.30U/x2-144[»]
4U55X-ray3.20U/x2-144[»]
4U56X-ray3.45U/x2-144[»]
4U6FX-ray3.10U/x2-144[»]
4V6Ielectron microscopy-S1-144[»]
4V7RX-ray4.00M1-144[»]
4V88X-ray3.00T1-144[»]
4V8Yelectron microscopy4.30T1-144[»]
4V8Zelectron microscopy6.60T1-144[»]
4V92electron microscopy3.70T3-144[»]
ProteinModelPortaliP07280.
SMRiP07280. Positions 2-144.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S19e family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000013102.
HOGENOMiHOG000230649.
InParanoidiP07280.
KOiK02966.
OMAiWAPFVKT.
OrthoDBiEOG71CFZT.

Family and domain databases

InterProiIPR001266. Ribosomal_S19e.
IPR018277. Ribosomal_S19e_CS.
[Graphical view]
PANTHERiPTHR11710. PTHR11710. 1 hit.
PfamiPF01090. Ribosomal_S19e. 1 hit.
[Graphical view]
ProDomiPD003854. Ribosomal_S19e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00628. RIBOSOMAL_S19E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07280-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPGVSVRDVA AQDFINAYAS FLQRQGKLEV PGYVDIVKTS SGNEMPPQDA
60 70 80 90 100
EGWFYKRAAS VARHIYMRKQ VGVGKLNKLY GGAKSRGVRP YKHIDASGSI
110 120 130 140
NRKVLQALEK IGIVEISPKG GRRISENGQR DLDRIAAQTL EEDE
Length:144
Mass (Da):15,917
Last modified:January 23, 2007 - v2
Checksum:iFE06D94B993B11B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02635 Genomic DNA. Translation: CAA26482.1.
X95258 Genomic DNA. Translation: CAA64549.1.
Z74863 Genomic DNA. Translation: CAA99140.1.
BK006948 Genomic DNA. Translation: DAA10663.1.
PIRiS05868. R3BY9E.
RefSeqiNP_014520.1. NM_001183375.1.

Genome annotation databases

EnsemblFungiiYOL121C; YOL121C; YOL121C.
GeneIDi854028.
KEGGisce:YOL121C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02635 Genomic DNA. Translation: CAA26482.1.
X95258 Genomic DNA. Translation: CAA64549.1.
Z74863 Genomic DNA. Translation: CAA99140.1.
BK006948 Genomic DNA. Translation: DAA10663.1.
PIRiS05868. R3BY9E.
RefSeqiNP_014520.1. NM_001183375.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10U1-144[»]
3J6Yelectron microscopy6.10U1-144[»]
3J77electron microscopy6.20T1-144[»]
3J78electron microscopy6.30T1-144[»]
3V88X-ray3.00T1-144[»]
4U3MX-ray3.00U/x2-144[»]
4U3NX-ray3.20U/x2-144[»]
4U3UX-ray2.90U/x2-144[»]
4U4NX-ray3.10U/x2-144[»]
4U4OX-ray3.60U/x2-144[»]
4U4QX-ray3.00U/x2-144[»]
4U4RX-ray2.80U/x2-144[»]
4U4UX-ray3.00U/x2-144[»]
4U4YX-ray3.20U/x2-144[»]
4U4ZX-ray3.10U/x2-144[»]
4U50X-ray3.20U/x2-144[»]
4U51X-ray3.20U/x2-144[»]
4U52X-ray3.00U/x2-144[»]
4U53X-ray3.30U/x2-144[»]
4U55X-ray3.20U/x2-144[»]
4U56X-ray3.45U/x2-144[»]
4U6FX-ray3.10U/x2-144[»]
4V6Ielectron microscopy-S1-144[»]
4V7RX-ray4.00M1-144[»]
4V88X-ray3.00T1-144[»]
4V8Yelectron microscopy4.30T1-144[»]
4V8Zelectron microscopy6.60T1-144[»]
4V92electron microscopy3.70T3-144[»]
ProteinModelPortaliP07280.
SMRiP07280. Positions 2-144.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34280. 36 interactions.
IntActiP07280. 31 interactions.
MINTiMINT-4502132.
STRINGi4932.YOL121C.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL121C; YOL121C; YOL121C.
GeneIDi854028.
KEGGisce:YOL121C.

Organism-specific databases

SGDiS000005481. RPS19A.

Phylogenomic databases

GeneTreeiENSGT00390000013102.
HOGENOMiHOG000230649.
InParanoidiP07280.
KOiK02966.
OMAiWAPFVKT.
OrthoDBiEOG71CFZT.

Enzyme and pathway databases

BioCyciYEAST:G3O-33517-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_248935. Ribosomal scanning and start codon recognition.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257608. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_257951. Peptide chain elongation.

Miscellaneous databases

NextBioi975574.

Gene expression databases

GenevestigatoriP07280.

Family and domain databases

InterProiIPR001266. Ribosomal_S19e.
IPR018277. Ribosomal_S19e_CS.
[Graphical view]
PANTHERiPTHR11710. PTHR11710. 1 hit.
PfamiPF01090. Ribosomal_S19e. 1 hit.
[Graphical view]
ProDomiPD003854. Ribosomal_S19e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00628. RIBOSOMAL_S19E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and organization of two linked ribosomal protein genes in yeast."
    Molenaar C.M.T., Woudt L.P., Jansen A.E.M., Mager W.H., Planta R.J., Donovan D.M., Pearson N.J.
    Nucleic Acids Res. 12:7345-7358(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "DNA sequence analysis of a 10 624 bp fragment of the left arm of chromosome XV from Saccharomyces cerevisiae reveals a RNA binding protein, a mitochondrial protein, two ribosomal proteins and two new open reading frames."
    Lafuente M.J., Gamo F.-J., Gancedo C.
    Yeast 12:1041-1045(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast."
    Otaka E., Higo K., Osawa S.
    Biochemistry 21:4545-4550(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-50.
  6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Specific role for yeast homologs of the Diamond Blackfan anemia-associated Rps19 protein in ribosome synthesis."
    Leger-Silvestre I., Caffrey J.M., Dawaliby R., Alvarez-Arias D.A., Gas N., Bertolone S.J., Gleizes P.E., Ellis S.R.
    J. Biol. Chem. 280:38177-38185(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN RIBOSOME SYNTHESIS, MUTAGENESIS OF ILE-65, DISRUPTION PHENOTYPE.
  10. "Molecular basis of Diamond-Blackfan anemia: structure and function analysis of RPS19."
    Gregory L.A., Aguissa-Toure A.H., Pinaud N., Legrand P., Gleizes P.E., Fribourg S.
    Nucleic Acids Res. 35:5913-5921(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ILE-15; ARG-57; ARG-63; ILE-65; ARG-102 AND ARG-122.

Entry informationi

Entry nameiRS19A_YEAST
AccessioniPrimary (citable) accession number: P07280
Secondary accession number(s): D6W1U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: February 4, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 29000 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S19 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.