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Protein

40S ribosomal protein S19-A

Gene

RPS19A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for proper maturation of the small (40S) ribosomal subunit. Binds to 40s pre-ribosomal particles, probably required after association of NOC4 but before association of ENP1, TSR1 and RIO2 with 20/21S pre-rRNA.2 Publications

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • ribosomal small subunit assembly Source: GO_Central
  • ribosomal small subunit biogenesis Source: SGD
  • rRNA export from nucleus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Biological processi

Ribosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-33517-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S19-A
Alternative name(s):
RP55A
S16a
YP45
YS16A
Gene namesi
Name:RPS19A
Synonyms:RP55A, RPS16AA
Ordered Locus Names:YOL121C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL121C.
SGDiS000005481. RPS19A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Disruption of a single RPS19 gene reduces cell growth; a double disruption is lethal. Depletion experiments show the proteins are required for correct maturation of precursor rRNA to generate the 18S small rRNA. A specific site between the 18S and 5.8S precursors (site A2 in ETS1) is not cleaved in disruption mutants. Partially assembled ribosomes are retained in the nucleolus rather than being exported to the cytoplasm. All effects are exacerbated in the double disruption. Increases association of NOC4 with 20S/21S pre-rRNA, decreases association of ENP1, TSR1 and RIO2 with 20S/21S pre-rRNA.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15I → F: Partial loss of function; decreased 18S rRNA, decreases binding to 20S pre-rRNA complex, slow growth in double RPS19A/RPS19B mutant. 1 Publication1
Mutagenesisi57R → E or Q: Loss of mature 18S rRNA, protein doesn't bind 20S pre-RNA complex. Lethal in double RPS19A/RPS19B mutant. 1 Publication1
Mutagenesisi63R → E: Loss of mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. 1 Publication1
Mutagenesisi65I → P: Lethal in double RPS19A/RPS19B mutant, considerable decrease in 18S rRNA production. 2 Publications1
Mutagenesisi102R → E: Decreases mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. 1 Publication1
Mutagenesisi122R → E: Decreases mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001538352 – 14440S ribosomal protein S19-AAdd BLAST143

Proteomic databases

MaxQBiP07280.
PRIDEiP07280.
TopDownProteomicsiP07280.

PTM databases

iPTMnetiP07280.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi34280. 36 interactors.
IntActiP07280. 32 interactors.
MINTiMINT-4502132.

Structurei

Secondary structure

1144
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 8Combined sources3
Helixi11 – 25Combined sources15
Beta strandi34 – 36Combined sources3
Beta strandi39 – 42Combined sources4
Beta strandi51 – 53Combined sources3
Helixi54 – 66Combined sources13
Beta strandi68 – 71Combined sources4
Helixi73 – 80Combined sources8
Beta strandi83 – 86Combined sources4
Beta strandi89 – 93Combined sources5
Helixi98 – 110Combined sources13
Beta strandi113 – 115Combined sources3
Beta strandi120 – 124Combined sources5
Helixi126 – 141Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10191-144[»]
3J6Yelectron microscopy6.10191-144[»]
3J77electron microscopy6.20191-144[»]
3J78electron microscopy6.30191-144[»]
3V88X-ray3.00T1-144[»]
4U3MX-ray3.00C9/c92-144[»]
4U3NX-ray3.20C9/c92-144[»]
4U3UX-ray2.90C9/c92-144[»]
4U4NX-ray3.10C9/c92-144[»]
4U4OX-ray3.60C9/c92-144[»]
4U4QX-ray3.00C9/c92-144[»]
4U4RX-ray2.80C9/c92-144[»]
4U4UX-ray3.00C9/c92-144[»]
4U4YX-ray3.20C9/c92-144[»]
4U4ZX-ray3.10C9/c92-144[»]
4U50X-ray3.20C9/c92-144[»]
4U51X-ray3.20C9/c92-144[»]
4U52X-ray3.00C9/c92-144[»]
4U53X-ray3.30C9/c92-144[»]
4U55X-ray3.20C9/c92-144[»]
4U56X-ray3.45C9/c92-144[»]
4U6FX-ray3.10C9/c92-144[»]
4V6Ielectron microscopy8.80AS1-144[»]
4V7RX-ray4.00AM/CM1-144[»]
4V88X-ray3.00AT/CT1-144[»]
4V8Yelectron microscopy4.30AT1-144[»]
4V8Zelectron microscopy6.60AT1-144[»]
4V92electron microscopy3.70T3-144[»]
5DATX-ray3.15C9/c92-144[»]
5DC3X-ray3.25C9/c92-144[»]
5FCIX-ray3.40C9/c92-144[»]
5FCJX-ray3.10C9/c92-144[»]
5I4LX-ray3.10C9/c92-144[»]
5JUOelectron microscopy4.00QB1-144[»]
5JUPelectron microscopy3.50QB1-144[»]
5JUSelectron microscopy4.20QB1-144[»]
5JUTelectron microscopy4.00QB1-144[»]
5JUUelectron microscopy4.00QB1-144[»]
ProteinModelPortaliP07280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S19e family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000013102.
HOGENOMiHOG000230649.
InParanoidiP07280.
KOiK02966.
OMAiHIYLRKD.
OrthoDBiEOG092C5NFY.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001266. Ribosomal_S19e.
IPR018277. Ribosomal_S19e_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11710. PTHR11710. 1 hit.
PfamiPF01090. Ribosomal_S19e. 1 hit.
[Graphical view]
ProDomiPD003854. Ribosomal_S19e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM01413. Ribosomal_S19e. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00628. RIBOSOMAL_S19E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07280-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGVSVRDVA AQDFINAYAS FLQRQGKLEV PGYVDIVKTS SGNEMPPQDA
60 70 80 90 100
EGWFYKRAAS VARHIYMRKQ VGVGKLNKLY GGAKSRGVRP YKHIDASGSI
110 120 130 140
NRKVLQALEK IGIVEISPKG GRRISENGQR DLDRIAAQTL EEDE
Length:144
Mass (Da):15,917
Last modified:January 23, 2007 - v2
Checksum:iFE06D94B993B11B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02635 Genomic DNA. Translation: CAA26482.1.
X95258 Genomic DNA. Translation: CAA64549.1.
Z74863 Genomic DNA. Translation: CAA99140.1.
BK006948 Genomic DNA. Translation: DAA10663.1.
PIRiS05868. R3BY9E.
RefSeqiNP_014520.1. NM_001183375.1.

Genome annotation databases

EnsemblFungiiYOL121C; YOL121C; YOL121C.
GeneIDi854028.
KEGGisce:YOL121C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02635 Genomic DNA. Translation: CAA26482.1.
X95258 Genomic DNA. Translation: CAA64549.1.
Z74863 Genomic DNA. Translation: CAA99140.1.
BK006948 Genomic DNA. Translation: DAA10663.1.
PIRiS05868. R3BY9E.
RefSeqiNP_014520.1. NM_001183375.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10191-144[»]
3J6Yelectron microscopy6.10191-144[»]
3J77electron microscopy6.20191-144[»]
3J78electron microscopy6.30191-144[»]
3V88X-ray3.00T1-144[»]
4U3MX-ray3.00C9/c92-144[»]
4U3NX-ray3.20C9/c92-144[»]
4U3UX-ray2.90C9/c92-144[»]
4U4NX-ray3.10C9/c92-144[»]
4U4OX-ray3.60C9/c92-144[»]
4U4QX-ray3.00C9/c92-144[»]
4U4RX-ray2.80C9/c92-144[»]
4U4UX-ray3.00C9/c92-144[»]
4U4YX-ray3.20C9/c92-144[»]
4U4ZX-ray3.10C9/c92-144[»]
4U50X-ray3.20C9/c92-144[»]
4U51X-ray3.20C9/c92-144[»]
4U52X-ray3.00C9/c92-144[»]
4U53X-ray3.30C9/c92-144[»]
4U55X-ray3.20C9/c92-144[»]
4U56X-ray3.45C9/c92-144[»]
4U6FX-ray3.10C9/c92-144[»]
4V6Ielectron microscopy8.80AS1-144[»]
4V7RX-ray4.00AM/CM1-144[»]
4V88X-ray3.00AT/CT1-144[»]
4V8Yelectron microscopy4.30AT1-144[»]
4V8Zelectron microscopy6.60AT1-144[»]
4V92electron microscopy3.70T3-144[»]
5DATX-ray3.15C9/c92-144[»]
5DC3X-ray3.25C9/c92-144[»]
5FCIX-ray3.40C9/c92-144[»]
5FCJX-ray3.10C9/c92-144[»]
5I4LX-ray3.10C9/c92-144[»]
5JUOelectron microscopy4.00QB1-144[»]
5JUPelectron microscopy3.50QB1-144[»]
5JUSelectron microscopy4.20QB1-144[»]
5JUTelectron microscopy4.00QB1-144[»]
5JUUelectron microscopy4.00QB1-144[»]
ProteinModelPortaliP07280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34280. 36 interactors.
IntActiP07280. 32 interactors.
MINTiMINT-4502132.

PTM databases

iPTMnetiP07280.

Proteomic databases

MaxQBiP07280.
PRIDEiP07280.
TopDownProteomicsiP07280.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL121C; YOL121C; YOL121C.
GeneIDi854028.
KEGGisce:YOL121C.

Organism-specific databases

EuPathDBiFungiDB:YOL121C.
SGDiS000005481. RPS19A.

Phylogenomic databases

GeneTreeiENSGT00390000013102.
HOGENOMiHOG000230649.
InParanoidiP07280.
KOiK02966.
OMAiHIYLRKD.
OrthoDBiEOG092C5NFY.

Enzyme and pathway databases

BioCyciYEAST:G3O-33517-MONOMER.

Miscellaneous databases

PROiP07280.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001266. Ribosomal_S19e.
IPR018277. Ribosomal_S19e_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11710. PTHR11710. 1 hit.
PfamiPF01090. Ribosomal_S19e. 1 hit.
[Graphical view]
ProDomiPD003854. Ribosomal_S19e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM01413. Ribosomal_S19e. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00628. RIBOSOMAL_S19E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRS19A_YEAST
AccessioniPrimary (citable) accession number: P07280
Secondary accession number(s): D6W1U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 29000 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S19 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.