Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

40S ribosomal protein S19-A

Gene

RPS19A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (PubMed:22096102). eS19 is required for proper maturation of the small (40S) ribosomal subunit. Binds to 40S pre-ribosomal particles, probably required after association of NOC4 but before association of ENP1, TSR1 and RIO2 with 20/21S pre-rRNA (PubMed:16159874, PubMed:17726054).1 Publication2 Publications

Miscellaneous

Present with 29000 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eS19 in yeast.Curated

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • ribosomal small subunit assembly Source: GO_Central
  • ribosomal small subunit biogenesis Source: SGD
  • rRNA export from nucleus Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein
Biological processRibosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-33517-MONOMER
ReactomeiR-SCE-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane
R-SCE-72689 Formation of a pool of free 40S subunits
R-SCE-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-SCE-72702 Ribosomal scanning and start codon recognition
R-SCE-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S19-A1 Publication
Alternative name(s):
RP55A
S16a
Small ribosomal subunit protein eS19-A1 Publication
YP45
YS16A
Gene namesi
Name:RPS19A1 Publication
Synonyms:RP55A, RPS16AA
Ordered Locus Names:YOL121C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL121C
SGDiS000005481 RPS19A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Disruption of a single RPS19 gene reduces cell growth; a double disruption is lethal. Depletion experiments show the proteins are required for correct maturation of precursor rRNA to generate the 18S small rRNA. A specific site between the 18S and 5.8S precursors (site A2 in ETS1) is not cleaved in disruption mutants. Partially assembled ribosomes are retained in the nucleolus rather than being exported to the cytoplasm. All effects are exacerbated in the double disruption. Increases association of NOC4 with 20S/21S pre-rRNA, decreases association of ENP1, TSR1 and RIO2 with 20S/21S pre-rRNA.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15I → F: Partial loss of function; decreased 18S rRNA, decreases binding to 20S pre-rRNA complex, slow growth in double RPS19A/RPS19B mutant. 1 Publication1
Mutagenesisi57R → E or Q: Loss of mature 18S rRNA, protein doesn't bind 20S pre-RNA complex. Lethal in double RPS19A/RPS19B mutant. 1 Publication1
Mutagenesisi63R → E: Loss of mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. 1 Publication1
Mutagenesisi65I → P: Lethal in double RPS19A/RPS19B mutant, considerable decrease in 18S rRNA production. 2 Publications1
Mutagenesisi102R → E: Decreases mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. 1 Publication1
Mutagenesisi122R → E: Decreases mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001538352 – 14440S ribosomal protein S19-AAdd BLAST143

Proteomic databases

MaxQBiP07280
PaxDbiP07280
PRIDEiP07280
TopDownProteomicsiP07280

PTM databases

iPTMnetiP07280

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi34280, 182 interactors
IntActiP07280, 33 interactors
MINTiP07280
STRINGi4932.YOL121C

Structurei

Secondary structure

1144
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 8Combined sources3
Helixi11 – 25Combined sources15
Turni32 – 36Combined sources5
Turni50 – 52Combined sources3
Helixi54 – 66Combined sources13
Beta strandi68 – 71Combined sources4
Helixi73 – 80Combined sources8
Beta strandi82 – 86Combined sources5
Beta strandi89 – 94Combined sources6
Helixi98 – 110Combined sources13
Beta strandi113 – 117Combined sources5
Turni118 – 120Combined sources3
Beta strandi121 – 124Combined sources4
Helixi126 – 142Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10191-144[»]
3J6Yelectron microscopy6.10191-144[»]
3J77electron microscopy6.20191-144[»]
3J78electron microscopy6.30191-144[»]
4U3MX-ray3.00C9/c92-144[»]
4U3NX-ray3.20C9/c92-144[»]
4U3UX-ray2.90C9/c92-144[»]
4U4NX-ray3.10C9/c92-144[»]
4U4OX-ray3.60C9/c92-144[»]
4U4QX-ray3.00C9/c92-144[»]
4U4RX-ray2.80C9/c92-144[»]
4U4UX-ray3.00C9/c92-144[»]
4U4YX-ray3.20C9/c92-144[»]
4U4ZX-ray3.10C9/c92-144[»]
4U50X-ray3.20C9/c92-144[»]
4U51X-ray3.20C9/c92-144[»]
4U52X-ray3.00C9/c92-144[»]
4U53X-ray3.30C9/c92-144[»]
4U55X-ray3.20C9/c92-144[»]
4U56X-ray3.45C9/c92-144[»]
4U6FX-ray3.10C9/c92-144[»]
4V6Ielectron microscopy8.80AS1-144[»]
4V7RX-ray4.00AM/CM1-144[»]
4V88X-ray3.00AT/CT1-144[»]
4V8Yelectron microscopy4.30AT1-144[»]
4V8Zelectron microscopy6.60AT1-144[»]
4V92electron microscopy3.70T3-144[»]
5DATX-ray3.15C9/c92-144[»]
5DC3X-ray3.25C9/c92-144[»]
5DGEX-ray3.45C9/c92-144[»]
5DGFX-ray3.30C9/c92-144[»]
5DGVX-ray3.10C9/c92-144[»]
5FCIX-ray3.40C9/c92-144[»]
5FCJX-ray3.10C9/c92-144[»]
5I4LX-ray3.10C9/c92-144[»]
5JUOelectron microscopy4.00QB1-144[»]
5JUPelectron microscopy3.50QB1-144[»]
5JUSelectron microscopy4.20QB1-144[»]
5JUTelectron microscopy4.00QB1-144[»]
5JUUelectron microscopy4.00QB1-144[»]
5LYBX-ray3.25C9/c92-144[»]
5M1Jelectron microscopy3.30T22-144[»]
5MC6electron microscopy3.80I1-144[»]
5MEIX-ray3.50U/c92-144[»]
5NDGX-ray3.70C9/c92-144[»]
5NDVX-ray3.30C9/c92-144[»]
5NDWX-ray3.70C9/c92-144[»]
5OBMX-ray3.40C9/c92-144[»]
5ON6X-ray3.10U/c92-144[»]
5TBWX-ray3.00U/c92-144[»]
5TGAX-ray3.30C9/c92-144[»]
5TGMX-ray3.50C9/c92-144[»]
6EMLelectron microscopy3.60I1-144[»]
6FAIelectron microscopy3.40T1-144[»]
ProteinModelPortaliP07280
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000013102
HOGENOMiHOG000230649
InParanoidiP07280
KOiK02966
OMAiYIDGPVG
OrthoDBiEOG092C5NFY

Family and domain databases

Gene3Di1.10.10.2700, 1 hit
InterProiView protein in InterPro
IPR001266 Ribosomal_S19e
IPR018277 Ribosomal_S19e_CS
IPR038111 Ribosomal_S19e_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR11710 PTHR11710, 1 hit
PfamiView protein in Pfam
PF01090 Ribosomal_S19e, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD003854 Ribosomal_S19e, 1 hit
SMARTiView protein in SMART
SM01413 Ribosomal_S19e, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00628 RIBOSOMAL_S19E, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07280-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGVSVRDVA AQDFINAYAS FLQRQGKLEV PGYVDIVKTS SGNEMPPQDA
60 70 80 90 100
EGWFYKRAAS VARHIYMRKQ VGVGKLNKLY GGAKSRGVRP YKHIDASGSI
110 120 130 140
NRKVLQALEK IGIVEISPKG GRRISENGQR DLDRIAAQTL EEDE
Length:144
Mass (Da):15,917
Last modified:January 23, 2007 - v2
Checksum:iFE06D94B993B11B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02635 Genomic DNA Translation: CAA26482.1
X95258 Genomic DNA Translation: CAA64549.1
Z74863 Genomic DNA Translation: CAA99140.1
BK006948 Genomic DNA Translation: DAA10663.1
PIRiS05868 R3BY9E
RefSeqiNP_014520.1, NM_001183375.1

Genome annotation databases

EnsemblFungiiYOL121C; YOL121C; YOL121C
GeneIDi854028
KEGGisce:YOL121C

Similar proteinsi

Entry informationi

Entry nameiRS19A_YEAST
AccessioniPrimary (citable) accession number: P07280
Secondary accession number(s): D6W1U7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health