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P07280 (RS19A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
40S ribosomal protein S19-A
Alternative name(s):
RP55A
S16a
YP45
YS16A
Gene names
Name:RPS19A
Synonyms:RP55A, RPS16AA
Ordered Locus Names:YOL121C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length144 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for proper maturation of the small (40S) ribosomal subunit. Binds to 40s pre-ribosomal particles, probably required after association of NOC4 but before association of ENP1, TSR1 and RIO2 with 20/21S pre-rRNA. Ref.9 Ref.10

Subunit structure

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6

Subcellular location

Cytoplasm Ref.7.

Disruption phenotype

Disruption of a single RPS19 gene reduces cell growth; a double disruption is lethal. Depletion experiments show the proteins are required for correct maturation of precursor rRNA to generate the 18S small rRNA. A specific site between the 18S and 5.8S precursors (site A2 in ETS1) is not cleaved in disruption mutants. Partially assembled ribosomes are retained in the nucleolus rather than being exported to the cytoplasm. All effects are exacerbated in the double disruption. Increases association of NOC4 with 20S/21S pre-rRNA, decreases association of ENP1, TSR1 and RIO2 with 20S/21S pre-rRNA. Ref.9 Ref.10

Miscellaneous

Present with 29000 molecules/cell in log phase SD medium.

There are 2 genes for S19 in yeast.

Sequence similarities

Belongs to the ribosomal protein S19e family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 14414340S ribosomal protein S19-A
PRO_0000153835

Experimental info

Mutagenesis151I → F: Partial loss of function; decreased 18S rRNA, decreases binding to 20S pre-rRNA complex, slow growth in double RPS19A/RPS19B mutant. Ref.10
Mutagenesis571R → E or Q: Loss of mature 18S rRNA, protein doesn't bind 20S pre-RNA complex. Lethal in double RPS19A/RPS19B mutant. Ref.10
Mutagenesis631R → E: Loss of mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. Ref.10
Mutagenesis651I → P: Lethal in double RPS19A/RPS19B mutant, considerable decrease in 18S rRNA production. Ref.9 Ref.10
Mutagenesis1021R → E: Decreases mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. Ref.10
Mutagenesis1221R → E: Decreases mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. Ref.10

Secondary structure

.......................... 144
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07280 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: FE06D94B993B11B2

FASTA14415,917
        10         20         30         40         50         60 
MPGVSVRDVA AQDFINAYAS FLQRQGKLEV PGYVDIVKTS SGNEMPPQDA EGWFYKRAAS 

        70         80         90        100        110        120 
VARHIYMRKQ VGVGKLNKLY GGAKSRGVRP YKHIDASGSI NRKVLQALEK IGIVEISPKG 

       130        140 
GRRISENGQR DLDRIAAQTL EEDE 

« Hide

References

« Hide 'large scale' references
[1]"Structure and organization of two linked ribosomal protein genes in yeast."
Molenaar C.M.T., Woudt L.P., Jansen A.E.M., Mager W.H., Planta R.J., Donovan D.M., Pearson N.J.
Nucleic Acids Res. 12:7345-7358(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"DNA sequence analysis of a 10 624 bp fragment of the left arm of chromosome XV from Saccharomyces cerevisiae reveals a RNA binding protein, a mitochondrial protein, two ribosomal proteins and two new open reading frames."
Lafuente M.J., Gamo F.-J., Gancedo C.
Yeast 12:1041-1045(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast."
Otaka E., Higo K., Osawa S.
Biochemistry 21:4545-4550(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-50.
[6]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Specific role for yeast homologs of the Diamond Blackfan anemia-associated Rps19 protein in ribosome synthesis."
Leger-Silvestre I., Caffrey J.M., Dawaliby R., Alvarez-Arias D.A., Gas N., Bertolone S.J., Gleizes P.E., Ellis S.R.
J. Biol. Chem. 280:38177-38185(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN RIBOSOME SYNTHESIS, MUTAGENESIS OF ILE-65, DISRUPTION PHENOTYPE.
[10]"Molecular basis of Diamond-Blackfan anemia: structure and function analysis of RPS19."
Gregory L.A., Aguissa-Toure A.H., Pinaud N., Legrand P., Gleizes P.E., Fribourg S.
Nucleic Acids Res. 35:5913-5921(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ILE-15; ARG-57; ARG-63; ILE-65; ARG-102 AND ARG-122.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X02635 Genomic DNA. Translation: CAA26482.1.
X95258 Genomic DNA. Translation: CAA64549.1.
Z74863 Genomic DNA. Translation: CAA99140.1.
BK006948 Genomic DNA. Translation: DAA10663.1.
PIRR3BY9E. S05868.
RefSeqNP_014520.1. NM_001183375.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3IZBelectron microscopy-S1-144[»]
3O2ZX-ray4.00M1-144[»]
3O30X-ray4.00M1-144[»]
3U5CX-ray3.00T1-144[»]
3U5GX-ray3.00T1-144[»]
4BYLelectron microscopy4.30T1-144[»]
4BYTelectron microscopy6.60T1-144[»]
ProteinModelPortalP07280.
SMRP07280. Positions 2-144.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34280. 35 interactions.
IntActP07280. 31 interactions.
MINTMINT-4502132.
STRING4932.YOL121C.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOL121C; YOL121C; YOL121C.
GeneID854028.
KEGGsce:YOL121C.

Organism-specific databases

SGDS000005481. RPS19A.

Phylogenomic databases

GeneTreeENSGT00390000013102.
HOGENOMHOG000230649.
KOK02966.
OMACQSLEKI.
OrthoDBEOG71CFZT.

Enzyme and pathway databases

BioCycYEAST:G3O-33517-MONOMER.

Gene expression databases

GenevestigatorP07280.

Family and domain databases

InterProIPR001266. Ribosomal_S19e.
IPR018277. Ribosomal_S19e_CS.
[Graphical view]
PANTHERPTHR11710. PTHR11710. 1 hit.
PfamPF01090. Ribosomal_S19e. 1 hit.
[Graphical view]
ProDomPD003854. Ribosomal_S19e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00628. RIBOSOMAL_S19E. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975574.

Entry information

Entry nameRS19A_YEAST
AccessionPrimary (citable) accession number: P07280
Secondary accession number(s): D6W1U7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references