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P07280

- RS19A_YEAST

UniProt

P07280 - RS19A_YEAST

Protein

40S ribosomal protein S19-A

Gene

RPS19A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Required for proper maturation of the small (40S) ribosomal subunit. Binds to 40s pre-ribosomal particles, probably required after association of NOC4 but before association of ENP1, TSR1 and RIO2 with 20/21S pre-rRNA.2 Publications

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. ribosomal small subunit biogenesis Source: SGD
    3. rRNA export from nucleus Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Biological processi

    Ribosome biogenesis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33517-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    40S ribosomal protein S19-A
    Alternative name(s):
    RP55A
    S16a
    YP45
    YS16A
    Gene namesi
    Name:RPS19A
    Synonyms:RP55A, RPS16AA
    Ordered Locus Names:YOL121C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    SGDiS000005481. RPS19A.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosolic small ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Disruption of a single RPS19 gene reduces cell growth; a double disruption is lethal. Depletion experiments show the proteins are required for correct maturation of precursor rRNA to generate the 18S small rRNA. A specific site between the 18S and 5.8S precursors (site A2 in ETS1) is not cleaved in disruption mutants. Partially assembled ribosomes are retained in the nucleolus rather than being exported to the cytoplasm. All effects are exacerbated in the double disruption. Increases association of NOC4 with 20S/21S pre-rRNA, decreases association of ENP1, TSR1 and RIO2 with 20S/21S pre-rRNA.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi15 – 151I → F: Partial loss of function; decreased 18S rRNA, decreases binding to 20S pre-rRNA complex, slow growth in double RPS19A/RPS19B mutant. 1 Publication
    Mutagenesisi57 – 571R → E or Q: Loss of mature 18S rRNA, protein doesn't bind 20S pre-RNA complex. Lethal in double RPS19A/RPS19B mutant. 1 Publication
    Mutagenesisi63 – 631R → E: Loss of mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. 1 Publication
    Mutagenesisi65 – 651I → P: Lethal in double RPS19A/RPS19B mutant, considerable decrease in 18S rRNA production. 2 Publications
    Mutagenesisi102 – 1021R → E: Decreases mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. 1 Publication
    Mutagenesisi122 – 1221R → E: Decreases mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 14414340S ribosomal protein S19-APRO_0000153835Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP07280.

    Interactioni

    Subunit structurei

    Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi34280. 35 interactions.
    IntActiP07280. 31 interactions.
    MINTiMINT-4502132.
    STRINGi4932.YOL121C.

    Structurei

    Secondary structure

    1
    144
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 83
    Helixi11 – 2515
    Turni31 – 355
    Beta strandi51 – 533
    Helixi54 – 6613
    Beta strandi68 – 703
    Helixi73 – 808
    Beta strandi83 – 864
    Beta strandi89 – 935
    Helixi98 – 11013
    Beta strandi113 – 1153
    Beta strandi118 – 1214
    Helixi126 – 14217

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VW9electron microscopy6.10U1-144[»]
    1VWVelectron microscopy6.10U1-144[»]
    3IZBelectron microscopy-S1-144[»]
    3O2ZX-ray4.00M1-144[»]
    3O30X-ray4.00M1-144[»]
    3U5CX-ray3.00T1-144[»]
    3U5GX-ray3.00T1-144[»]
    4BYLelectron microscopy4.30T1-144[»]
    4BYTelectron microscopy6.60T1-144[»]
    4CUYelectron microscopy3.70T3-144[»]
    ProteinModelPortaliP07280.
    SMRiP07280. Positions 2-144.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein S19e family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00390000013102.
    HOGENOMiHOG000230649.
    KOiK02966.
    OMAiHIYLRKD.
    OrthoDBiEOG71CFZT.

    Family and domain databases

    InterProiIPR001266. Ribosomal_S19e.
    IPR018277. Ribosomal_S19e_CS.
    [Graphical view]
    PANTHERiPTHR11710. PTHR11710. 1 hit.
    PfamiPF01090. Ribosomal_S19e. 1 hit.
    [Graphical view]
    ProDomiPD003854. Ribosomal_S19e. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    PROSITEiPS00628. RIBOSOMAL_S19E. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07280-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGVSVRDVA AQDFINAYAS FLQRQGKLEV PGYVDIVKTS SGNEMPPQDA    50
    EGWFYKRAAS VARHIYMRKQ VGVGKLNKLY GGAKSRGVRP YKHIDASGSI 100
    NRKVLQALEK IGIVEISPKG GRRISENGQR DLDRIAAQTL EEDE 144
    Length:144
    Mass (Da):15,917
    Last modified:January 23, 2007 - v2
    Checksum:iFE06D94B993B11B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02635 Genomic DNA. Translation: CAA26482.1.
    X95258 Genomic DNA. Translation: CAA64549.1.
    Z74863 Genomic DNA. Translation: CAA99140.1.
    BK006948 Genomic DNA. Translation: DAA10663.1.
    PIRiS05868. R3BY9E.
    RefSeqiNP_014520.1. NM_001183375.1.

    Genome annotation databases

    EnsemblFungiiYOL121C; YOL121C; YOL121C.
    GeneIDi854028.
    KEGGisce:YOL121C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02635 Genomic DNA. Translation: CAA26482.1 .
    X95258 Genomic DNA. Translation: CAA64549.1 .
    Z74863 Genomic DNA. Translation: CAA99140.1 .
    BK006948 Genomic DNA. Translation: DAA10663.1 .
    PIRi S05868. R3BY9E.
    RefSeqi NP_014520.1. NM_001183375.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VW9 electron microscopy 6.10 U 1-144 [» ]
    1VWV electron microscopy 6.10 U 1-144 [» ]
    3IZB electron microscopy - S 1-144 [» ]
    3O2Z X-ray 4.00 M 1-144 [» ]
    3O30 X-ray 4.00 M 1-144 [» ]
    3U5C X-ray 3.00 T 1-144 [» ]
    3U5G X-ray 3.00 T 1-144 [» ]
    4BYL electron microscopy 4.30 T 1-144 [» ]
    4BYT electron microscopy 6.60 T 1-144 [» ]
    4CUY electron microscopy 3.70 T 3-144 [» ]
    ProteinModelPortali P07280.
    SMRi P07280. Positions 2-144.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34280. 35 interactions.
    IntActi P07280. 31 interactions.
    MINTi MINT-4502132.
    STRINGi 4932.YOL121C.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOL121C ; YOL121C ; YOL121C .
    GeneIDi 854028.
    KEGGi sce:YOL121C.

    Organism-specific databases

    SGDi S000005481. RPS19A.

    Phylogenomic databases

    GeneTreei ENSGT00390000013102.
    HOGENOMi HOG000230649.
    KOi K02966.
    OMAi HIYLRKD.
    OrthoDBi EOG71CFZT.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33517-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    NextBioi 975574.

    Gene expression databases

    Genevestigatori P07280.

    Family and domain databases

    InterProi IPR001266. Ribosomal_S19e.
    IPR018277. Ribosomal_S19e_CS.
    [Graphical view ]
    PANTHERi PTHR11710. PTHR11710. 1 hit.
    Pfami PF01090. Ribosomal_S19e. 1 hit.
    [Graphical view ]
    ProDomi PD003854. Ribosomal_S19e. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    PROSITEi PS00628. RIBOSOMAL_S19E. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and organization of two linked ribosomal protein genes in yeast."
      Molenaar C.M.T., Woudt L.P., Jansen A.E.M., Mager W.H., Planta R.J., Donovan D.M., Pearson N.J.
      Nucleic Acids Res. 12:7345-7358(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "DNA sequence analysis of a 10 624 bp fragment of the left arm of chromosome XV from Saccharomyces cerevisiae reveals a RNA binding protein, a mitochondrial protein, two ribosomal proteins and two new open reading frames."
      Lafuente M.J., Gamo F.-J., Gancedo C.
      Yeast 12:1041-1045(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast."
      Otaka E., Higo K., Osawa S.
      Biochemistry 21:4545-4550(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-50.
    6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Specific role for yeast homologs of the Diamond Blackfan anemia-associated Rps19 protein in ribosome synthesis."
      Leger-Silvestre I., Caffrey J.M., Dawaliby R., Alvarez-Arias D.A., Gas N., Bertolone S.J., Gleizes P.E., Ellis S.R.
      J. Biol. Chem. 280:38177-38185(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN RIBOSOME SYNTHESIS, MUTAGENESIS OF ILE-65, DISRUPTION PHENOTYPE.
    10. "Molecular basis of Diamond-Blackfan anemia: structure and function analysis of RPS19."
      Gregory L.A., Aguissa-Toure A.H., Pinaud N., Legrand P., Gleizes P.E., Fribourg S.
      Nucleic Acids Res. 35:5913-5921(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ILE-15; ARG-57; ARG-63; ILE-65; ARG-102 AND ARG-122.

    Entry informationi

    Entry nameiRS19A_YEAST
    AccessioniPrimary (citable) accession number: P07280
    Secondary accession number(s): D6W1U7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 29000 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for S19 in yeast.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3