Reviewed, UniProtKB/Swiss-Prot P07279 (RL18_YEAST)

Names and origin

Protein names

Recommended name:
    60S ribosomal protein L18
Alternative name(s):
    RP28

Gene names

Name:	RPL18A
Synonyms:	RP28A
Ordered Locus Names:	YOL120C
AND
Name:	RPL18B
Synonyms:	RP28B
Ordered Locus Names:	YNL301C
ORF Names:	N0425

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with NAP1. Ref.6 Ref.11
Subcellular location	Cytoplasm Ref.7.
Miscellaneous	Present with 63700 molecules/cell in log phase SD medium. Ref.8 There are 2 genes for L18 in yeast.
Sequence similarities	Belongs to the ribosomal protein L18e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	translation Ref.6 Traceable author statement. Source: SGD
Cellular component	cytosolic large ribosomal subunit Ref.6 Traceable author statement. Source: SGD
Molecular function	structural constituent of ribosome Ref.6 Traceable author statement. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	60S ribosomal protein L18		PRO_0000132784
Amino acid modifications
	Modified residue	86	1	Phosphothreonine Ref.10
	Modified residue	167	1	Phosphoserine Ref.10 Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P07279-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: D097B187F369EACD	FASTA	186	20,563
	10 20 30 40 50 60 MGIDHTSKQH KRSGHRTAPK SDNVYLKLLV KLYTFLARRT DAPFNKVVLK ALFLSKINRP 70 80 90 100 110 120 PVSVSRIARA LKQEGAANKT VVVVGTVTDD ARIFEFPKTT VAALRFTAGA RAKIVKAGGE 130 140 150 160 170 180 CITLDQLAVR APKGQNTLIL RGPRNSREAV RHFGMGPHKG KAPRILSTGR KFERARGRRR SKGFKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and organization of two linked ribosomal protein genes in yeast." Molenaar C.M.T., Woudt L.P., Jansen A.E.M., Mager W.H., Planta R.J., Donovan D.M., Pearson N.J. Nucleic Acids Res. 12:7345-7358(1984) [PubMed: 6387623] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RPL18A AND RPL18B).
[2]	"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed: 8553702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RPL18B). Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (RPL18B). Strain: ATCC 96604 / S288c / FY1679.
[4]	"DNA sequence analysis of a 10 624 bp fragment of the left arm of chromosome XV from Saccharomyces cerevisiae reveals a RNA binding protein, a mitochondrial protein, two ribosomal proteins and two new open reading frames." Lafuente M.J., Gamo F.-J., Gancedo C. Yeast 12:1041-1045(1996) [PubMed: 8896268] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RPL18A). Strain: ATCC 96604 / S288c / FY1679.
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (RPL18A). Strain: ATCC 96604 / S288c / FY1679.
[6]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167, MASS SPECTROMETRY.
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-86 AND SER-167, MASS SPECTROMETRY.
[11]	"Phosphorylation by casein kinase 2 regulates Nap1 localization and function." Calvert M.E.K., Keck K.M., Ptak C., Shabanowitz J., Hunt D.F., Pemberton L.F. Mol. Cell. Biol. 28:1313-1325(2008) [PubMed: 18086883] [Abstract] Cited for: INTERACTION WITH NAP1, MASS SPECTROMETRY.
[12]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed: 11701127] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 22-141, ELECTRON MICROSCOPY.
[13]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed: 14976550] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 22-141, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X01099 Genomic DNA. Translation: CAA25573.1.
X02635 Genomic DNA. Translation: CAA26481.1.
X01100 Genomic DNA. Translation: CAA25574.1.
U23084 Genomic DNA. Translation: AAC49097.1.
Z71577 Genomic DNA. Translation: CAA96219.1.
X95258 Genomic DNA. Translation: CAA64550.1.
Z74862 Genomic DNA. Translation: CAA99139.1.

PIR

R5BY8E. S05867.

RefSeq

NP_014098.1.
NP_014521.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	O	22-141	[»]
1S1I	electron microscopy	11.70	O	22-141	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4554N.

IntAct

P07279. 73 interactions.

STRING

P07279.

Proteomic databases

PRIDE

P07279.

Genome annotation databases

Ensembl

YNL301C; YNL301C; YNL301C; Saccharomyces cerevisiae. [Genome view]
YOL120C; YOL120C; YOL120C; Saccharomyces cerevisiae. [Genome view]

GeneID

854029.
855415.

KEGG

sce:YNL301C.
sce:YOL120C.

Organism-specific databases

CYGD

YNL301c.
YOL120c.

SGD

S000005480. RPL18A.
S000005245. RPL18B.

Phylogenomic databases

HOGENOM

HBG499813.

OMA

DAPFNKV.

OrthoDB

EOG98SJ98.

Gene expression databases

ArrayExpress

P07279.

Genevestigator

P07279.

GermOnline

YNL301C. Saccharomyces cerevisiae.
YOL120C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000039. Ribosomal_L18e.
[Graphical view]

PANTHER

PTHR10934. Ribosomal_L18e. 1 hit.

Pfam

PF00828. Ribosomal_L18e. 1 hit.
[Graphical view]

PROSITE

PS01106. RIBOSOMAL_L18E. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

975577.

Entry information

Entry name

RL18_YEAST

Accession

Primary (citable) accession number: P07279

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	December 15, 2009
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)