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P07277

- KIME_YEAST

UniProt

P07277 - KIME_YEAST

Protein

Mevalonate kinase

Gene

ERG12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    May contribute to the regulation of the isoprenoid and sterol pathway in living cells.
    RAR (regulation of autonomous replication) is a protein whose activity increases the mitotic stability of plasmids.

    Catalytic activityi

    ATP + (R)-mevalonate = ADP + (R)-5-phosphomevalonate.

    Enzyme regulationi

    Farnesyl pyrophosphate and geranyl pyrophosphate inhibit mevalonate kinase by binding competitively at the ATP-binding site.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei138 – 1381ATPBy similarity
    Active sitei202 – 2021Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi141 – 15111ATPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. mevalonate kinase activity Source: SGD

    GO - Biological processi

    1. ergosterol biosynthetic process Source: SGD
    2. farnesyl diphosphate biosynthetic process, mevalonate pathway Source: SGD
    3. isopentenyl diphosphate biosynthetic process, mevalonate pathway Source: SGD

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-651.
    YEAST:YMR208W-MONOMER.
    UniPathwayiUPA00057; UER00098.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mevalonate kinase (EC:2.7.1.36)
    Short name:
    MK
    Short name:
    MvK
    Alternative name(s):
    Ergosterol biosynthesis protein 12
    Regulation of autonomous replication protein 1
    Gene namesi
    Name:ERG12
    Synonyms:RAR1
    Ordered Locus Names:YMR208W
    ORF Names:YM8261.02
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR208w.
    SGDiS000004821. ERG12.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 443443Mevalonate kinasePRO_0000156662Add
    BLAST

    Proteomic databases

    MaxQBiP07277.
    PaxDbiP07277.
    PeptideAtlasiP07277.

    Expressioni

    Gene expression databases

    GenevestigatoriP07277.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi35386. 49 interactions.
    IntActiP07277. 3 interactions.
    MINTiMINT-4498479.
    STRINGi4932.YMR208W.

    Structurei

    3D structure databases

    ProteinModelPortaliP07277.
    SMRiP07277. Positions 1-409.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1577.
    GeneTreeiENSGT00390000011860.
    HOGENOMiHOG000188935.
    KOiK00869.
    OMAiGERMIHG.
    OrthoDBiEOG7C5MM9.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.70.890. 1 hit.
    InterProiIPR013750. GHMP_kinase_C_dom.
    IPR006204. GHMP_kinase_N_dom.
    IPR006203. GHMP_knse_ATP-bd_CS.
    IPR006205. Mev_gal_kin.
    IPR006206. Mevalonate/galactokinase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view]
    PANTHERiPTHR10457:SF4. PTHR10457:SF4. 1 hit.
    PfamiPF00288. GHMP_kinases_N. 1 hit.
    [Graphical view]
    PRINTSiPR00959. MEVGALKINASE.
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55060. SSF55060. 1 hit.
    TIGRFAMsiTIGR00549. mevalon_kin. 1 hit.
    PROSITEiPS00627. GHMP_KINASES_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07277-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLPFLTSAP GKVIIFGEHS AVYNKPAVAA SVSALRTYLL ISESSAPDTI    50
    ELDFPDISFN HKWSINDFNA ITEDQVNSQK LAKAQQATDG LSQELVSLLD 100
    PLLAQLSESF HYHAAFCFLY MFVCLCPHAK NIKFSLKSTL PIGAGLGSSA 150
    SISVSLALAM AYLGGLIGSN DLEKLSENDK HIVNQWAFIG EKCIHGTPSG 200
    IDNAVATYGN ALLFEKDSHN GTINTNNFKF LDDFPAIPMI LTYTRIPRST 250
    KDLVARVRVL VTEKFPEVMK PILDAMGECA LQGLEIMTKL SKCKGTDDEA 300
    VETNNELYEQ LLELIRINHG LLVSIGVSHP GLELIKNLSD DLRIGSTKLT 350
    GAGGGGCSLT LLRRDITQEQ IDSFKKKLQD DFSYETFETD LGGTGCCLLS 400
    AKNLNKDLKI KSLVFQLFEN KTTTKQQIDD LLLPGNTNLP WTS 443
    Length:443
    Mass (Da):48,460
    Last modified:April 1, 1988 - v1
    Checksum:i8B24052A72C97280
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06114 Genomic DNA. Translation: CAA29487.1.
    X55875 Genomic DNA. Translation: CAA39359.1.
    Z49809 Genomic DNA. Translation: CAA89923.1.
    D78165 Genomic DNA. Translation: BAA24409.1.
    BK006946 Genomic DNA. Translation: DAA10107.1.
    PIRiS05875. BVBYR1.
    RefSeqiNP_013935.1. NM_001182715.1.

    Genome annotation databases

    EnsemblFungiiYMR208W; YMR208W; YMR208W.
    GeneIDi855248.
    KEGGisce:YMR208W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06114 Genomic DNA. Translation: CAA29487.1 .
    X55875 Genomic DNA. Translation: CAA39359.1 .
    Z49809 Genomic DNA. Translation: CAA89923.1 .
    D78165 Genomic DNA. Translation: BAA24409.1 .
    BK006946 Genomic DNA. Translation: DAA10107.1 .
    PIRi S05875. BVBYR1.
    RefSeqi NP_013935.1. NM_001182715.1.

    3D structure databases

    ProteinModelPortali P07277.
    SMRi P07277. Positions 1-409.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35386. 49 interactions.
    IntActi P07277. 3 interactions.
    MINTi MINT-4498479.
    STRINGi 4932.YMR208W.

    Proteomic databases

    MaxQBi P07277.
    PaxDbi P07277.
    PeptideAtlasi P07277.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR208W ; YMR208W ; YMR208W .
    GeneIDi 855248.
    KEGGi sce:YMR208W.

    Organism-specific databases

    CYGDi YMR208w.
    SGDi S000004821. ERG12.

    Phylogenomic databases

    eggNOGi COG1577.
    GeneTreei ENSGT00390000011860.
    HOGENOMi HOG000188935.
    KOi K00869.
    OMAi GERMIHG.
    OrthoDBi EOG7C5MM9.

    Enzyme and pathway databases

    UniPathwayi UPA00057 ; UER00098 .
    BioCyci MetaCyc:MONOMER-651.
    YEAST:YMR208W-MONOMER.

    Miscellaneous databases

    NextBioi 978816.
    PROi P07277.

    Gene expression databases

    Genevestigatori P07277.

    Family and domain databases

    Gene3Di 3.30.230.10. 1 hit.
    3.30.70.890. 1 hit.
    InterProi IPR013750. GHMP_kinase_C_dom.
    IPR006204. GHMP_kinase_N_dom.
    IPR006203. GHMP_knse_ATP-bd_CS.
    IPR006205. Mev_gal_kin.
    IPR006206. Mevalonate/galactokinase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view ]
    PANTHERi PTHR10457:SF4. PTHR10457:SF4. 1 hit.
    Pfami PF00288. GHMP_kinases_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00959. MEVGALKINASE.
    SUPFAMi SSF54211. SSF54211. 1 hit.
    SSF55060. SSF55060. 1 hit.
    TIGRFAMsi TIGR00549. mevalon_kin. 1 hit.
    PROSITEi PS00627. GHMP_KINASES_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mutations that increase the mitotic stability of minichromosomes in yeast: characterization of RAR1."
      Kearsey S.E., Edwards J.
      Mol. Gen. Genet. 210:509-517(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Nucleotide sequence of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase."
      Oulmouden A., Karst F.
      Curr. Genet. 19:9-14(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Saito A., Kazuta Y., Kondo H., Tanabe T.
      Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-246.
      Strain: SP1.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiKIME_YEAST
    AccessioniPrimary (citable) accession number: P07277
    Secondary accession number(s): D6W033
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 138 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3