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P07277 (KIME_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mevalonate kinase

Short name=MK
Short name=MvK
EC=2.7.1.36
Alternative name(s):
Ergosterol biosynthesis protein 12
Regulation of autonomous replication protein 1
Gene names
Name:ERG12
Synonyms:RAR1
Ordered Locus Names:YMR208W
ORF Names:YM8261.02
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length443 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May contribute to the regulation of the isoprenoid and sterol pathway in living cells.

RAR (regulation of autonomous replication) is a protein whose activity increases the mitotic stability of plasmids.

Catalytic activity

ATP + (R)-mevalonate = ADP + (R)-5-phosphomevalonate.

Enzyme regulation

Farnesyl pyrophosphate and geranyl pyrophosphate inhibit mevalonate kinase by binding competitively at the ATP-binding site.

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Miscellaneous

Present with 3300 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the GHMP kinase family. Mevalonate kinase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 443443Mevalonate kinase
PRO_0000156662

Regions

Nucleotide binding141 – 15111ATP By similarity

Sites

Active site2021Proton acceptor By similarity
Binding site1381ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P07277 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 8B24052A72C97280

FASTA44348,460
        10         20         30         40         50         60 
MSLPFLTSAP GKVIIFGEHS AVYNKPAVAA SVSALRTYLL ISESSAPDTI ELDFPDISFN 

        70         80         90        100        110        120 
HKWSINDFNA ITEDQVNSQK LAKAQQATDG LSQELVSLLD PLLAQLSESF HYHAAFCFLY 

       130        140        150        160        170        180 
MFVCLCPHAK NIKFSLKSTL PIGAGLGSSA SISVSLALAM AYLGGLIGSN DLEKLSENDK 

       190        200        210        220        230        240 
HIVNQWAFIG EKCIHGTPSG IDNAVATYGN ALLFEKDSHN GTINTNNFKF LDDFPAIPMI 

       250        260        270        280        290        300 
LTYTRIPRST KDLVARVRVL VTEKFPEVMK PILDAMGECA LQGLEIMTKL SKCKGTDDEA 

       310        320        330        340        350        360 
VETNNELYEQ LLELIRINHG LLVSIGVSHP GLELIKNLSD DLRIGSTKLT GAGGGGCSLT 

       370        380        390        400        410        420 
LLRRDITQEQ IDSFKKKLQD DFSYETFETD LGGTGCCLLS AKNLNKDLKI KSLVFQLFEN 

       430        440 
KTTTKQQIDD LLLPGNTNLP WTS 

« Hide

References

« Hide 'large scale' references
[1]"Mutations that increase the mitotic stability of minichromosomes in yeast: characterization of RAR1."
Kearsey S.E., Edwards J.
Mol. Gen. Genet. 210:509-517(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Nucleotide sequence of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase."
Oulmouden A., Karst F.
Curr. Genet. 19:9-14(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]Saito A., Kazuta Y., Kondo H., Tanabe T.
Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-246.
Strain: SP1.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X06114 Genomic DNA. Translation: CAA29487.1.
X55875 Genomic DNA. Translation: CAA39359.1.
Z49809 Genomic DNA. Translation: CAA89923.1.
D78165 Genomic DNA. Translation: BAA24409.1.
BK006946 Genomic DNA. Translation: DAA10107.1.
PIRBVBYR1. S05875.
RefSeqNP_013935.1. NM_001182715.1.

3D structure databases

ProteinModelPortalP07277.
SMRP07277. Positions 1-409.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35386. 49 interactions.
IntActP07277. 3 interactions.
MINTMINT-4498479.
STRING4932.YMR208W.

Proteomic databases

MaxQBP07277.
PaxDbP07277.
PeptideAtlasP07277.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR208W; YMR208W; YMR208W.
GeneID855248.
KEGGsce:YMR208W.

Organism-specific databases

CYGDYMR208w.
SGDS000004821. ERG12.

Phylogenomic databases

eggNOGCOG1577.
GeneTreeENSGT00390000011860.
HOGENOMHOG000188935.
KOK00869.
OMAGERMIHG.
OrthoDBEOG7C5MM9.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-651.
YEAST:YMR208W-MONOMER.
UniPathwayUPA00057; UER00098.

Gene expression databases

GenevestigatorP07277.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
InterProIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006205. Mev_gal_kin.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERPTHR10457:SF4. PTHR10457:SF4. 1 hit.
PfamPF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PRINTSPR00959. MEVGALKINASE.
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsTIGR00549. mevalon_kin. 1 hit.
PROSITEPS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978816.
PROP07277.

Entry information

Entry nameKIME_YEAST
AccessionPrimary (citable) accession number: P07277
Secondary accession number(s): D6W033
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: June 11, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways