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Protein

Mevalonate kinase

Gene

ERG12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May contribute to the regulation of the isoprenoid and sterol pathway in living cells.
RAR (regulation of autonomous replication) is a protein whose activity increases the mitotic stability of plasmids.

Catalytic activityi

ATP + (R)-mevalonate = ADP + (R)-5-phosphomevalonate.

Enzyme regulationi

Farnesyl pyrophosphate and geranyl pyrophosphate inhibit mevalonate kinase by binding competitively at the ATP-binding site.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei138 – 1381ATPBy similarity
Active sitei202 – 2021Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi141 – 15111ATPBy similarityAdd
BLAST

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • mevalonate kinase activity Source: SGD

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD
  • farnesyl diphosphate biosynthetic process, mevalonate pathway Source: SGD
  • isopentenyl diphosphate biosynthetic process, mevalonate pathway Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-651.
YEAST:YMR208W-MONOMER.
ReactomeiREACT_283157. Cholesterol biosynthesis.
UniPathwayiUPA00057; UER00098.

Names & Taxonomyi

Protein namesi
Recommended name:
Mevalonate kinase (EC:2.7.1.36)
Short name:
MK
Short name:
MvK
Alternative name(s):
Ergosterol biosynthesis protein 12
Regulation of autonomous replication protein 1
Gene namesi
Name:ERG12
Synonyms:RAR1
Ordered Locus Names:YMR208W
ORF Names:YM8261.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYMR208w.
EuPathDBiFungiDB:YMR208W.
SGDiS000004821. ERG12.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443Mevalonate kinasePRO_0000156662Add
BLAST

Proteomic databases

MaxQBiP07277.
PaxDbiP07277.
PeptideAtlasiP07277.

Expressioni

Gene expression databases

GenevestigatoriP07277.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi35386. 50 interactions.
IntActiP07277. 3 interactions.
MINTiMINT-4498479.
STRINGi4932.YMR208W.

Structurei

3D structure databases

ProteinModelPortaliP07277.
SMRiP07277. Positions 1-409.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1577.
GeneTreeiENSGT00390000011860.
HOGENOMiHOG000188935.
InParanoidiP07277.
KOiK00869.
OMAiGERMIHG.
OrthoDBiEOG7C5MM9.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006205. Mev_gal_kin.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457:SF4. PTHR10457:SF4. 1 hit.
PfamiPF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PRINTSiPR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00549. mevalon_kin. 1 hit.
PROSITEiPS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07277-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLPFLTSAP GKVIIFGEHS AVYNKPAVAA SVSALRTYLL ISESSAPDTI
60 70 80 90 100
ELDFPDISFN HKWSINDFNA ITEDQVNSQK LAKAQQATDG LSQELVSLLD
110 120 130 140 150
PLLAQLSESF HYHAAFCFLY MFVCLCPHAK NIKFSLKSTL PIGAGLGSSA
160 170 180 190 200
SISVSLALAM AYLGGLIGSN DLEKLSENDK HIVNQWAFIG EKCIHGTPSG
210 220 230 240 250
IDNAVATYGN ALLFEKDSHN GTINTNNFKF LDDFPAIPMI LTYTRIPRST
260 270 280 290 300
KDLVARVRVL VTEKFPEVMK PILDAMGECA LQGLEIMTKL SKCKGTDDEA
310 320 330 340 350
VETNNELYEQ LLELIRINHG LLVSIGVSHP GLELIKNLSD DLRIGSTKLT
360 370 380 390 400
GAGGGGCSLT LLRRDITQEQ IDSFKKKLQD DFSYETFETD LGGTGCCLLS
410 420 430 440
AKNLNKDLKI KSLVFQLFEN KTTTKQQIDD LLLPGNTNLP WTS
Length:443
Mass (Da):48,460
Last modified:April 1, 1988 - v1
Checksum:i8B24052A72C97280
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06114 Genomic DNA. Translation: CAA29487.1.
X55875 Genomic DNA. Translation: CAA39359.1.
Z49809 Genomic DNA. Translation: CAA89923.1.
D78165 Genomic DNA. Translation: BAA24409.1.
BK006946 Genomic DNA. Translation: DAA10107.1.
PIRiS05875. BVBYR1.
RefSeqiNP_013935.1. NM_001182715.1.

Genome annotation databases

EnsemblFungiiYMR208W; YMR208W; YMR208W.
GeneIDi855248.
KEGGisce:YMR208W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06114 Genomic DNA. Translation: CAA29487.1.
X55875 Genomic DNA. Translation: CAA39359.1.
Z49809 Genomic DNA. Translation: CAA89923.1.
D78165 Genomic DNA. Translation: BAA24409.1.
BK006946 Genomic DNA. Translation: DAA10107.1.
PIRiS05875. BVBYR1.
RefSeqiNP_013935.1. NM_001182715.1.

3D structure databases

ProteinModelPortaliP07277.
SMRiP07277. Positions 1-409.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35386. 50 interactions.
IntActiP07277. 3 interactions.
MINTiMINT-4498479.
STRINGi4932.YMR208W.

Proteomic databases

MaxQBiP07277.
PaxDbiP07277.
PeptideAtlasiP07277.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR208W; YMR208W; YMR208W.
GeneIDi855248.
KEGGisce:YMR208W.

Organism-specific databases

CYGDiYMR208w.
EuPathDBiFungiDB:YMR208W.
SGDiS000004821. ERG12.

Phylogenomic databases

eggNOGiCOG1577.
GeneTreeiENSGT00390000011860.
HOGENOMiHOG000188935.
InParanoidiP07277.
KOiK00869.
OMAiGERMIHG.
OrthoDBiEOG7C5MM9.

Enzyme and pathway databases

UniPathwayiUPA00057; UER00098.
BioCyciMetaCyc:MONOMER-651.
YEAST:YMR208W-MONOMER.
ReactomeiREACT_283157. Cholesterol biosynthesis.

Miscellaneous databases

NextBioi978816.
PROiP07277.

Gene expression databases

GenevestigatoriP07277.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006205. Mev_gal_kin.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457:SF4. PTHR10457:SF4. 1 hit.
PfamiPF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PRINTSiPR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00549. mevalon_kin. 1 hit.
PROSITEiPS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mutations that increase the mitotic stability of minichromosomes in yeast: characterization of RAR1."
    Kearsey S.E., Edwards J.
    Mol. Gen. Genet. 210:509-517(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase."
    Oulmouden A., Karst F.
    Curr. Genet. 19:9-14(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Saito A., Kazuta Y., Kondo H., Tanabe T.
    Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-246.
    Strain: SP1.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKIME_YEAST
AccessioniPrimary (citable) accession number: P07277
Secondary accession number(s): D6W033
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: April 29, 2015
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.