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Protein

Mevalonate kinase

Gene

ERG12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May contribute to the regulation of the isoprenoid and sterol pathway in living cells.
RAR (regulation of autonomous replication) is a protein whose activity increases the mitotic stability of plasmids.

Catalytic activityi

ATP + (R)-mevalonate = ADP + (R)-5-phosphomevalonate.

Enzyme regulationi

Farnesyl pyrophosphate and geranyl pyrophosphate inhibit mevalonate kinase by binding competitively at the ATP-binding site.

Pathwayi: isopentenyl diphosphate biosynthesis via mevalonate pathway

This protein is involved in step 1 of the subpathway that synthesizes isopentenyl diphosphate from (R)-mevalonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Mevalonate kinase (ERG12)
  2. Phosphomevalonate kinase (ERG8)
  3. Diphosphomevalonate decarboxylase (MVD1)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via mevalonate pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from (R)-mevalonate, the pathway isopentenyl diphosphate biosynthesis via mevalonate pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei138ATPBy similarity1
Active sitei202Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi141 – 151ATPBy similarityAdd BLAST11

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • mevalonate kinase activity Source: SGD

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD
  • farnesyl diphosphate biosynthetic process, mevalonate pathway Source: SGD
  • isopentenyl diphosphate biosynthetic process, mevalonate pathway Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YMR208W-MONOMER.
YEAST:YMR208W-MONOMER.
ReactomeiR-SCE-191273. Cholesterol biosynthesis.
UniPathwayiUPA00057; UER00098.

Names & Taxonomyi

Protein namesi
Recommended name:
Mevalonate kinase (EC:2.7.1.36)
Short name:
MK
Short name:
MvK
Alternative name(s):
Ergosterol biosynthesis protein 12
Regulation of autonomous replication protein 1
Gene namesi
Name:ERG12
Synonyms:RAR1
Ordered Locus Names:YMR208W
ORF Names:YM8261.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR208W.
SGDiS000004821. ERG12.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001566621 – 443Mevalonate kinaseAdd BLAST443

Proteomic databases

MaxQBiP07277.
PRIDEiP07277.

PTM databases

iPTMnetiP07277.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi35386. 49 interactors.
IntActiP07277. 3 interactors.
MINTiMINT-4498479.

Structurei

3D structure databases

ProteinModelPortaliP07277.
SMRiP07277.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000011860.
HOGENOMiHOG000188935.
InParanoidiP07277.
KOiK00869.
OMAiCMYALAP.
OrthoDBiEOG092C5126.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006205. Mev_gal_kin.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457. PTHR10457. 1 hit.
PTHR10457:SF4. PTHR10457:SF4. 1 hit.
PfamiPF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PRINTSiPR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00549. mevalon_kin. 1 hit.
PROSITEiPS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07277-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLPFLTSAP GKVIIFGEHS AVYNKPAVAA SVSALRTYLL ISESSAPDTI
60 70 80 90 100
ELDFPDISFN HKWSINDFNA ITEDQVNSQK LAKAQQATDG LSQELVSLLD
110 120 130 140 150
PLLAQLSESF HYHAAFCFLY MFVCLCPHAK NIKFSLKSTL PIGAGLGSSA
160 170 180 190 200
SISVSLALAM AYLGGLIGSN DLEKLSENDK HIVNQWAFIG EKCIHGTPSG
210 220 230 240 250
IDNAVATYGN ALLFEKDSHN GTINTNNFKF LDDFPAIPMI LTYTRIPRST
260 270 280 290 300
KDLVARVRVL VTEKFPEVMK PILDAMGECA LQGLEIMTKL SKCKGTDDEA
310 320 330 340 350
VETNNELYEQ LLELIRINHG LLVSIGVSHP GLELIKNLSD DLRIGSTKLT
360 370 380 390 400
GAGGGGCSLT LLRRDITQEQ IDSFKKKLQD DFSYETFETD LGGTGCCLLS
410 420 430 440
AKNLNKDLKI KSLVFQLFEN KTTTKQQIDD LLLPGNTNLP WTS
Length:443
Mass (Da):48,460
Last modified:April 1, 1988 - v1
Checksum:i8B24052A72C97280
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06114 Genomic DNA. Translation: CAA29487.1.
X55875 Genomic DNA. Translation: CAA39359.1.
Z49809 Genomic DNA. Translation: CAA89923.1.
D78165 Genomic DNA. Translation: BAA24409.1.
BK006946 Genomic DNA. Translation: DAA10107.1.
PIRiS05875. BVBYR1.
RefSeqiNP_013935.1. NM_001182715.1.

Genome annotation databases

EnsemblFungiiYMR208W; YMR208W; YMR208W.
GeneIDi855248.
KEGGisce:YMR208W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06114 Genomic DNA. Translation: CAA29487.1.
X55875 Genomic DNA. Translation: CAA39359.1.
Z49809 Genomic DNA. Translation: CAA89923.1.
D78165 Genomic DNA. Translation: BAA24409.1.
BK006946 Genomic DNA. Translation: DAA10107.1.
PIRiS05875. BVBYR1.
RefSeqiNP_013935.1. NM_001182715.1.

3D structure databases

ProteinModelPortaliP07277.
SMRiP07277.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35386. 49 interactors.
IntActiP07277. 3 interactors.
MINTiMINT-4498479.

PTM databases

iPTMnetiP07277.

Proteomic databases

MaxQBiP07277.
PRIDEiP07277.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR208W; YMR208W; YMR208W.
GeneIDi855248.
KEGGisce:YMR208W.

Organism-specific databases

EuPathDBiFungiDB:YMR208W.
SGDiS000004821. ERG12.

Phylogenomic databases

GeneTreeiENSGT00390000011860.
HOGENOMiHOG000188935.
InParanoidiP07277.
KOiK00869.
OMAiCMYALAP.
OrthoDBiEOG092C5126.

Enzyme and pathway databases

UniPathwayiUPA00057; UER00098.
BioCyciMetaCyc:YMR208W-MONOMER.
YEAST:YMR208W-MONOMER.
ReactomeiR-SCE-191273. Cholesterol biosynthesis.

Miscellaneous databases

PROiP07277.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006205. Mev_gal_kin.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457. PTHR10457. 1 hit.
PTHR10457:SF4. PTHR10457:SF4. 1 hit.
PfamiPF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PRINTSiPR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00549. mevalon_kin. 1 hit.
PROSITEiPS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKIME_YEAST
AccessioniPrimary (citable) accession number: P07277
Secondary accession number(s): D6W033
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 2, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.