Reviewed, UniProtKB/Swiss-Prot P07277 (KIME_YEAST)

Names and origin

Protein names

Recommended name:
    Mevalonate kinase
      Short name=MvK
      Short name=MK
    EC=2.7.1.36

Gene names

Name:	RAR1
Synonyms:	ERG12
Ordered Locus Names:	YMR208W
ORF Names:	YM8261.02

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May contribute to the regulation of the isoprenoid and sterol pathway in living cells. RAR (regulation of autonomous replication) is a protein whose activity increases the mitotic stability of plasmids.
Catalytic activity	ATP + (R)-mevalonate = ADP + (R)-5-phosphomevalonate.
Enzyme regulation	Farnesyl pyrophosphate and geranyl pyrophosphate inhibit mevalonate kinase by binding competitively at the ATP-binding site.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 3300 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the GHMP kinase family. Mevalonate kinase subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro phosphorylation Inferred from electronic annotation. Source: InterPro sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW mevalonate kinase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Mevalonate kinase		PRO_0000156662
Regions
	Nucleotide binding	141 – 151	11	ATP By similarity
Sites
	Binding site	138	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07277-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 8B24052A72C97280	FASTA	443	48,460
	10 20 30 40 50 60 MSLPFLTSAP GKVIIFGEHS AVYNKPAVAA SVSALRTYLL ISESSAPDTI ELDFPDISFN 70 80 90 100 110 120 HKWSINDFNA ITEDQVNSQK LAKAQQATDG LSQELVSLLD PLLAQLSESF HYHAAFCFLY 130 140 150 160 170 180 MFVCLCPHAK NIKFSLKSTL PIGAGLGSSA SISVSLALAM AYLGGLIGSN DLEKLSENDK 190 200 210 220 230 240 HIVNQWAFIG EKCIHGTPSG IDNAVATYGN ALLFEKDSHN GTINTNNFKF LDDFPAIPMI 250 260 270 280 290 300 LTYTRIPRST KDLVARVRVL VTEKFPEVMK PILDAMGECA LQGLEIMTKL SKCKGTDDEA 310 320 330 340 350 360 VETNNELYEQ LLELIRINHG LLVSIGVSHP GLELIKNLSD DLRIGSTKLT GAGGGGCSLT 370 380 390 400 410 420 LLRRDITQEQ IDSFKKKLQD DFSYETFETD LGGTGCCLLS AKNLNKDLKI KSLVFQLFEN 430 440 KTTTKQQIDD LLLPGNTNLP WTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mutations that increase the mitotic stability of minichromosomes in yeast: characterization of RAR1." Kearsey S.E., Edwards J. Mol. Gen. Genet. 210:509-517(1987) [PubMed: 3323847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase." Oulmouden A., Karst F. Curr. Genet. 19:9-14(1991) [PubMed: 1645230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saito A., Kazuta Y., Kondo H., Tanabe T. Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-246. Strain: SP1.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X06114 Genomic DNA. Translation: CAA29487.1. X55875 Genomic DNA. Translation: CAA39359.1. Z49809 Genomic DNA. Translation: CAA89923.1. D78165 Genomic DNA. Translation: BAA24409.1.
PIR	BVBYR1. S05875.
RefSeq	NP_013935.1.
3D structure databases
HSSP	HSSP built from PDB template 1KVK based on UniProtKB P17256.
ModBase	Search...
Protein-protein interaction databases
IntAct	P07277. 4 interactions.
Proteomic databases
PeptideAtlas	P07277.
PRIDE	P07277.
Genome annotation databases
Ensembl	YMR208W. Saccharomyces cerevisiae. [Contig view]
GeneID	855248.
GenomeReviews	Gene locus YMR208W in contig Z71257_GR.
KEGG	sce:YMR208W.
NMPDR	fig|4932.3.peg.4988.
Organism-specific databases
CYGD	YMR208w.
SGD	S000004821. ERG12.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P07277.
OMA	P07277. AYESERV.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-651.
BRENDA	2.7.1.36. 250.
Gene expression databases
ArrayExpress	P07277.
GermOnline	YMR208W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP_bd_CS. IPR006205. Mev_gal_kin. IPR006206. Mev_galkinase. IPR014721. Ribosomal_S5_D2-type_fold. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
PANTHER	PTHR10457:SF4. Mev_gal_kin. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PRINTS	PR00959. MEVGALKINASE.
TIGRFAMs	TIGR00549. mevalon_kin. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	978816.

Entry information

Entry name

KIME_YEAST

Accession

Primary (citable) accession number: P07277

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	June 16, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)