P07277 (KIME_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mevalonate kinase
Short name=MK
Short name=MvK
EC=2.7.1.36

Alternative name(s):
Ergosterol biosynthesis protein 12
Regulation of autonomous replication protein 1

Gene names

Name:	ERG12
Synonyms:	RAR1
Ordered Locus Names:	YMR208W
ORF Names:	YM8261.02

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May contribute to the regulation of the isoprenoid and sterol pathway in living cells. RAR (regulation of autonomous replication) is a protein whose activity increases the mitotic stability of plasmids.
Catalytic activity	ATP + (R)-mevalonate = ADP + (R)-5-phosphomevalonate.
Enzyme regulation	Farnesyl pyrophosphate and geranyl pyrophosphate inhibit mevalonate kinase by binding competitively at the ATP-binding site.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 3300 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the GHMP kinase family. Mevalonate kinase subfamily.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ergosterol biosynthetic process Inferred from mutant phenotype PubMed 200835. Source: SGD farnesyl diphosphate biosynthetic process, mevalonate pathway Inferred from mutant phenotype PubMed 200835. Source: SGD isopentenyl diphosphate biosynthetic process, mevalonate pathway Inferred from mutant phenotype PubMed 200835. Source: SGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW mevalonate kinase activity Inferred from direct assay PubMed 16477420. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Mevalonate kinase		PRO_0000156662
Regions
	Nucleotide binding	141 – 151	11	ATP By similarity
Sites
	Binding site	138	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07277 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 8B24052A72C97280	FASTA	443	48,460
	10 20 30 40 50 60 MSLPFLTSAP GKVIIFGEHS AVYNKPAVAA SVSALRTYLL ISESSAPDTI ELDFPDISFN 70 80 90 100 110 120 HKWSINDFNA ITEDQVNSQK LAKAQQATDG LSQELVSLLD PLLAQLSESF HYHAAFCFLY 130 140 150 160 170 180 MFVCLCPHAK NIKFSLKSTL PIGAGLGSSA SISVSLALAM AYLGGLIGSN DLEKLSENDK 190 200 210 220 230 240 HIVNQWAFIG EKCIHGTPSG IDNAVATYGN ALLFEKDSHN GTINTNNFKF LDDFPAIPMI 250 260 270 280 290 300 LTYTRIPRST KDLVARVRVL VTEKFPEVMK PILDAMGECA LQGLEIMTKL SKCKGTDDEA 310 320 330 340 350 360 VETNNELYEQ LLELIRINHG LLVSIGVSHP GLELIKNLSD DLRIGSTKLT GAGGGGCSLT 370 380 390 400 410 420 LLRRDITQEQ IDSFKKKLQD DFSYETFETD LGGTGCCLLS AKNLNKDLKI KSLVFQLFEN 430 440 KTTTKQQIDD LLLPGNTNLP WTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mutations that increase the mitotic stability of minichromosomes in yeast: characterization of RAR1." Kearsey S.E., Edwards J. Mol. Gen. Genet. 210:509-517(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase." Oulmouden A., Karst F. Curr. Genet. 19:9-14(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	Saito A., Kazuta Y., Kondo H., Tanabe T. Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-246. Strain: SP1.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06114 Genomic DNA. Translation: CAA29487.1. X55875 Genomic DNA. Translation: CAA39359.1. Z49809 Genomic DNA. Translation: CAA89923.1. D78165 Genomic DNA. Translation: BAA24409.1. BK006946 Genomic DNA. Translation: DAA10107.1.
PIR	BVBYR1. S05875.
RefSeq	NP_013935.1. NM_001182715.1.
3D structure databases
ProteinModelPortal	P07277.
SMR	P07277. Positions 1-409.
ModBase	Search...
Protein-protein interaction databases
IntAct	P07277. 3 interactions.
STRING	4932.YMR208W.
Proteomic databases
PaxDb	P07277.
PeptideAtlas	P07277.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR208W; YMR208W; YMR208W.
GeneID	855248.
KEGG	sce:YMR208W.
Organism-specific databases
CYGD	YMR208w.
SGD	S000004821. ERG12.
Phylogenomic databases
eggNOG	COG1577.
GeneTree	ENSGT00390000011860.
HOGENOM	HOG000188935.
KO	K00869.
OMA	ISCTCEK.
OrthoDB	EOG4WHCVG.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-651.
UniPathway	UPA00057; UER00098.
Gene expression databases
Genevestigator	P07277.
GermOnline	YMR208W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
InterPro	IPR006204. GHMP_kinase_N_dom. IPR006203. GHMP_knse_ATP-bd_CS. IPR006205. Mev_gal_kin. IPR006206. Mevalonate/galactokinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
PANTHER	PTHR10457:SF4. PTHR10457:SF4. 1 hit.
Pfam	PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PRINTS	PR00959. MEVGALKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00549. mevalon_kin. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978816.

Entry information

Entry name

KIME_YEAST

Accession

Primary (citable) accession number: P07277
Secondary accession number(s): D6W033

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	May 1, 2013
This is version 128 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program