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Protein

Mevalonate kinase

Gene

ERG12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May contribute to the regulation of the isoprenoid and sterol pathway in living cells.
RAR (regulation of autonomous replication) is a protein whose activity increases the mitotic stability of plasmids.

Miscellaneous

Present with 3300 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + (R)-mevalonate = ADP + (R)-5-phosphomevalonate.

Enzyme regulationi

Farnesyl pyrophosphate and geranyl pyrophosphate inhibit mevalonate kinase by binding competitively at the ATP-binding site.

Pathwayi: isopentenyl diphosphate biosynthesis via mevalonate pathway

This protein is involved in step 1 of the subpathway that synthesizes isopentenyl diphosphate from (R)-mevalonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Mevalonate kinase (ERG12)
  2. Phosphomevalonate kinase (ERG8)
  3. Diphosphomevalonate decarboxylase (MVD1)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via mevalonate pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from (R)-mevalonate, the pathway isopentenyl diphosphate biosynthesis via mevalonate pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei138ATPBy similarity1
Active sitei202Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi141 – 151ATPBy similarityAdd BLAST11

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • mevalonate kinase activity Source: SGD

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD
  • farnesyl diphosphate biosynthetic process, mevalonate pathway Source: SGD
  • isopentenyl diphosphate biosynthetic process, mevalonate pathway Source: SGD

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YMR208W-MONOMER
YEAST:YMR208W-MONOMER
ReactomeiR-SCE-191273 Cholesterol biosynthesis
UniPathwayiUPA00057; UER00098

Names & Taxonomyi

Protein namesi
Recommended name:
Mevalonate kinase (EC:2.7.1.36)
Short name:
MK
Short name:
MvK
Alternative name(s):
Ergosterol biosynthesis protein 12
Regulation of autonomous replication protein 1
Gene namesi
Name:ERG12
Synonyms:RAR1
Ordered Locus Names:YMR208W
ORF Names:YM8261.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR208W
SGDiS000004821 ERG12

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001566621 – 443Mevalonate kinaseAdd BLAST443

Proteomic databases

MaxQBiP07277
PaxDbiP07277
PRIDEiP07277

PTM databases

iPTMnetiP07277

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi35386, 96 interactors
IntActiP07277, 4 interactors
STRINGi4932.YMR208W

Structurei

3D structure databases

ProteinModelPortaliP07277
SMRiP07277
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000011860
HOGENOMiHOG000188935
InParanoidiP07277
KOiK00869
OMAiEGWKFWR
OrthoDBiEOG092C5126

Family and domain databases

Gene3Di3.30.230.10, 2 hits
3.30.70.890, 1 hit
InterProiView protein in InterPro
IPR036554 GHMP_kinase_C_sf
IPR006204 GHMP_kinase_N_dom
IPR006203 GHMP_knse_ATP-bd_CS
IPR006205 Mev_gal_kin
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
PfamiView protein in Pfam
PF00288 GHMP_kinases_N, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55060 SSF55060, 1 hit
TIGRFAMsiTIGR00549 mevalon_kin, 1 hit
PROSITEiView protein in PROSITE
PS00627 GHMP_KINASES_ATP, 1 hit

Sequencei

Sequence statusi: Complete.

P07277-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLPFLTSAP GKVIIFGEHS AVYNKPAVAA SVSALRTYLL ISESSAPDTI
60 70 80 90 100
ELDFPDISFN HKWSINDFNA ITEDQVNSQK LAKAQQATDG LSQELVSLLD
110 120 130 140 150
PLLAQLSESF HYHAAFCFLY MFVCLCPHAK NIKFSLKSTL PIGAGLGSSA
160 170 180 190 200
SISVSLALAM AYLGGLIGSN DLEKLSENDK HIVNQWAFIG EKCIHGTPSG
210 220 230 240 250
IDNAVATYGN ALLFEKDSHN GTINTNNFKF LDDFPAIPMI LTYTRIPRST
260 270 280 290 300
KDLVARVRVL VTEKFPEVMK PILDAMGECA LQGLEIMTKL SKCKGTDDEA
310 320 330 340 350
VETNNELYEQ LLELIRINHG LLVSIGVSHP GLELIKNLSD DLRIGSTKLT
360 370 380 390 400
GAGGGGCSLT LLRRDITQEQ IDSFKKKLQD DFSYETFETD LGGTGCCLLS
410 420 430 440
AKNLNKDLKI KSLVFQLFEN KTTTKQQIDD LLLPGNTNLP WTS
Length:443
Mass (Da):48,460
Last modified:April 1, 1988 - v1
Checksum:i8B24052A72C97280
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06114 Genomic DNA Translation: CAA29487.1
X55875 Genomic DNA Translation: CAA39359.1
Z49809 Genomic DNA Translation: CAA89923.1
D78165 Genomic DNA Translation: BAA24409.1
BK006946 Genomic DNA Translation: DAA10107.1
PIRiS05875 BVBYR1
RefSeqiNP_013935.1, NM_001182715.1

Genome annotation databases

EnsemblFungiiYMR208W; YMR208W; YMR208W
GeneIDi855248
KEGGisce:YMR208W

Similar proteinsi

Entry informationi

Entry nameiKIME_YEAST
AccessioniPrimary (citable) accession number: P07277
Secondary accession number(s): D6W033
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: March 28, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health