Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P07276

- RAD2_YEAST

UniProt

P07276 - RAD2_YEAST

Protein

DNA repair protein RAD2

Gene

RAD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 149 (01 Oct 2014)
      Sequence version 2 (01 Nov 1991)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Single-stranded DNA endonuclease involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Essential for the incision step of excision-repair.1 Publication

    Cofactori

    Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi30 – 301Magnesium 1By similarity
    Metal bindingi77 – 771Magnesium 1By similarity
    Metal bindingi792 – 7921Magnesium 1By similarity
    Metal bindingi794 – 7941Magnesium 1By similarity
    Metal bindingi813 – 8131Magnesium 2By similarity
    Metal bindingi815 – 8151Magnesium 2By similarity
    Metal bindingi864 – 8641Magnesium 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. single-stranded DNA binding Source: InterPro
    4. single-stranded DNA endodeoxyribonuclease activity Source: SGD

    GO - Biological processi

    1. DNA catabolic process, endonucleolytic Source: GOC
    2. nucleotide-excision repair, DNA incision, 3'-to lesion Source: SGD
    3. transcription from RNA polymerase II promoter Source: SGD

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30928-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA repair protein RAD2 (EC:3.1.-.-)
    Gene namesi
    Name:RAD2
    Ordered Locus Names:YGR258C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR258c.
    SGDiS000003490. RAD2.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleotide-excision repair factor 3 complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10311031DNA repair protein RAD2PRO_0000154035Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei118 – 1181Phosphoserine1 Publication
    Modified residuei367 – 3671Phosphoserine1 Publication
    Modified residuei583 – 5831Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP07276.
    PaxDbiP07276.
    PeptideAtlasiP07276.
    PRIDEiP07276.

    Expressioni

    Gene expression databases

    GenevestigatoriP07276.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SEN1Q004163EBI-14757,EBI-16945

    Protein-protein interaction databases

    BioGridi33510. 45 interactions.
    DIPiDIP-5869N.
    IntActiP07276. 1 interaction.
    MINTiMINT-683829.
    STRINGi4932.YGR258C.

    Structurei

    Secondary structure

    1
    1031
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi667 – 6693

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LOXNMR-B642-690[»]
    ProteinModelPortaliP07276.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 9595N-domainAdd
    BLAST
    Regioni756 – 884129I-domainAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0258.
    GeneTreeiENSGT00640000091542.
    HOGENOMiHOG000214817.
    KOiK10846.
    OMAiFQATMRD.
    OrthoDBiEOG7QK0MK.

    Family and domain databases

    Gene3Di3.40.50.1010. 2 hits.
    InterProiIPR020045. 5-3_exonuclease_C.
    IPR008918. HhH2.
    IPR029060. PIN_domain-like.
    IPR006086. XPG-I_dom.
    IPR001044. XPG/Rad2_eukaryotes.
    IPR019974. XPG_CS.
    IPR006085. XPG_DNA_repair_N.
    [Graphical view]
    PANTHERiPTHR11081:SF1. PTHR11081:SF1. 1 hit.
    PfamiPF00867. XPG_I. 1 hit.
    PF00752. XPG_N. 1 hit.
    [Graphical view]
    PRINTSiPR00066. XRODRMPGMNTG.
    SMARTiSM00279. HhH2. 1 hit.
    SM00484. XPGI. 1 hit.
    SM00485. XPGN. 1 hit.
    [Graphical view]
    SUPFAMiSSF47807. SSF47807. 2 hits.
    SSF88723. SSF88723. 2 hits.
    TIGRFAMsiTIGR00600. rad2. 1 hit.
    PROSITEiPS00841. XPG_1. 1 hit.
    PS00842. XPG_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07276-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGVHSFWDIA GPTARPVRLE SLEDKRMAVD ASIWIYQFLK AVRDQEGNAV     50
    KNSHITGFFR RICKLLYFGI RPVFVFDGGV PVLKRETIRQ RKERRQGKRE 100
    SAKSTARKLL ALQLQNGSND NVKNSTPSSG SSVQIFKPQD EWDLPDIPGF 150
    KYDKEDARVN SNKTFEKLMN SINGDGLEDI DLDTINPASA EFEELPKATQ 200
    YLILSSLRLK SRLRMGYSKE QLETIFPNSM DFSRFQIDMV KRRNFFTQKL 250
    INTTGFQDGG ASKLNEEVIN RISGQKSKEY KLTKTNNGWI LGLGANDGSD 300
    AQKAIVIDDK DAGALVKQLD SNAEDGDVLR WDDLEDNSLK IVRHESSNAT 350
    TAPQKRSNRS EDEGCDSDEC EWEEVELKPK NVKFVEDFSL KAARLPYMGQ 400
    SLNNAGSKSF LDKRHDQASP SKTTPTMRIS RISVEDDDED YLKQIEEIEM 450
    MEAVQLSKME KKPEADDKSK IAKPVTSKGT EARPPIVQYG LLGAQPDSKQ 500
    PYHVTNLNSK SESVIKRTSK TVLSEFRPPS QQEDKGAILT EGEQNLNFIS 550
    HKIPQFDFNN ENSLLFQKNT ESNVSQEATK EKSPIPEMPS WFSSTASQQL 600
    YNPYNTTNFV EDKNVRNEQE SGAETTNKGS SYELLTGLNA TEILERESEK 650
    ESSNDENKDD DLEVLSEELF EDVPTKSQIS KEAEDNDSRK VESINKEHRK 700
    PLIFDYDFSE DEEDNIVENM IKEQEEFDTF KNTTLSTSAE RNVAENAFVE 750
    DELFEQQMKD KRDSDEVTMD MIKEVQELLS RFGIPYITAP MEAEAQCAEL 800
    LQLNLVDGII TDDSDVFLFG GTKIYKNMFH EKNYVEFYDA ESILKLLGLD 850
    RKNMIELAQL LGSDYTNGLK GMGPVSSIEV IAEFGNLKNF KDWYNNGQFD 900
    KRKQETENKF EKDLRKKLVN NEIILDDDFP SVMVYDAYMR PEVDHDTTPF 950
    VWGVPDLDML RSFMKTQLGW PHEKSDEILI PLIRDVNKRK KKGKQKRINE 1000
    FFPREYISGD KKLNTSKRIS TATGKLKKRK M 1031
    Length:1,031
    Mass (Da):117,838
    Last modified:November 1, 1991 - v2
    Checksum:i682D4AECFBD7F0F3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti782 – 7821F → L in AAT92876. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10275 Genomic DNA. Translation: AAA66928.1.
    Y07777 Genomic DNA. Translation: CAA69080.1.
    Z73043 Genomic DNA. Translation: CAA97287.1.
    BK006941 Genomic DNA. Translation: DAA08349.1.
    AY692857 Genomic DNA. Translation: AAT92876.1.
    PIRiA29839.
    RefSeqiNP_011774.1. NM_001181387.1.

    Genome annotation databases

    EnsemblFungiiYGR258C; YGR258C; YGR258C.
    GeneIDi853174.
    KEGGisce:YGR258C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10275 Genomic DNA. Translation: AAA66928.1 .
    Y07777 Genomic DNA. Translation: CAA69080.1 .
    Z73043 Genomic DNA. Translation: CAA97287.1 .
    BK006941 Genomic DNA. Translation: DAA08349.1 .
    AY692857 Genomic DNA. Translation: AAT92876.1 .
    PIRi A29839.
    RefSeqi NP_011774.1. NM_001181387.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LOX NMR - B 642-690 [» ]
    ProteinModelPortali P07276.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33510. 45 interactions.
    DIPi DIP-5869N.
    IntActi P07276. 1 interaction.
    MINTi MINT-683829.
    STRINGi 4932.YGR258C.

    Proteomic databases

    MaxQBi P07276.
    PaxDbi P07276.
    PeptideAtlasi P07276.
    PRIDEi P07276.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR258C ; YGR258C ; YGR258C .
    GeneIDi 853174.
    KEGGi sce:YGR258C.

    Organism-specific databases

    CYGDi YGR258c.
    SGDi S000003490. RAD2.

    Phylogenomic databases

    eggNOGi COG0258.
    GeneTreei ENSGT00640000091542.
    HOGENOMi HOG000214817.
    KOi K10846.
    OMAi FQATMRD.
    OrthoDBi EOG7QK0MK.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30928-MONOMER.

    Miscellaneous databases

    NextBioi 973302.
    PROi P07276.

    Gene expression databases

    Genevestigatori P07276.

    Family and domain databases

    Gene3Di 3.40.50.1010. 2 hits.
    InterProi IPR020045. 5-3_exonuclease_C.
    IPR008918. HhH2.
    IPR029060. PIN_domain-like.
    IPR006086. XPG-I_dom.
    IPR001044. XPG/Rad2_eukaryotes.
    IPR019974. XPG_CS.
    IPR006085. XPG_DNA_repair_N.
    [Graphical view ]
    PANTHERi PTHR11081:SF1. PTHR11081:SF1. 1 hit.
    Pfami PF00867. XPG_I. 1 hit.
    PF00752. XPG_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00066. XRODRMPGMNTG.
    SMARTi SM00279. HhH2. 1 hit.
    SM00484. XPGI. 1 hit.
    SM00485. XPGN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47807. SSF47807. 2 hits.
    SSF88723. SSF88723. 2 hits.
    TIGRFAMsi TIGR00600. rad2. 1 hit.
    PROSITEi PS00841. XPG_1. 1 hit.
    PS00842. XPG_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The RAD2 gene of Saccharomyces cerevisiae: nucleotide sequence and transcript mapping."
      Nicolet C.M., Chenevert J.M., Friedberg E.C.
      Gene 36:225-234(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Nucleotide sequence, transcript mapping, and regulation of the RAD2 gene of Saccharomyces cerevisiae."
      Madura K., Prakash S.
      J. Bacteriol. 166:914-923(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Analysis of an 11.6 kb region from the right arm of chromosome VII of Saccharomyces cerevisiae between the RAD2 and the MES1 genes reveals the presence of three new genes."
      Clemente M.L., Sartori G., Cardazzo B., Carignani G.
      Yeast 13:287-290(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-51.
      Strain: ATCC 96604 / S288c / FY1679.
    7. "Yeast excision repair gene RAD2 encodes a single-stranded DNA endonuclease."
      Habraken Y., Sung P., Prakash L., Prakash S.
      Nature 366:365-368(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-583, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-118 AND SER-367, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRAD2_YEAST
    AccessioniPrimary (citable) accession number: P07276
    Secondary accession number(s): D6VV38, E9P8X9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 149 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 846 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3