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UniProtKB - P07276 (RAD2_YEAST)

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Protein

DNA repair protein RAD2

Gene

RAD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Single-stranded DNA endonuclease involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Essential for the incision step of excision-repair.1 Publication

Miscellaneous

Present with 846 molecules/cell in log phase SD medium.1 Publication

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi30Magnesium 1By similarity1
Metal bindingi77Magnesium 1By similarity1
Metal bindingi792Magnesium 1By similarity1
Metal bindingi794Magnesium 1By similarity1
Metal bindingi813Magnesium 2By similarity1
Metal bindingi815Magnesium 2By similarity1
Metal bindingi864Magnesium 2By similarity1

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

  • nucleotide-excision repair, DNA incision, 3'-to lesion Source: SGD
  • transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30928-MONOMER.
ReactomeiR-SCE-5696395. Formation of Incision Complex in GG-NER.
R-SCE-5696400. Dual Incision in GG-NER.
R-SCE-6782135. Dual incision in TC-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD2 (EC:3.1.-.-)
Gene namesi
Name:RAD2
Ordered Locus Names:YGR258C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR258C.
SGDiS000003490. RAD2.

Subcellular locationi

GO - Cellular componenti

  • nucleotide-excision repair factor 3 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001540351 – 1031DNA repair protein RAD2Add BLAST1031

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei118PhosphoserineCombined sources1
Modified residuei367PhosphoserineCombined sources1
Modified residuei583PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07276.
PRIDEiP07276.

PTM databases

iPTMnetiP07276.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SEN1Q004163EBI-14757,EBI-16945

Protein-protein interaction databases

BioGridi33510. 65 interactors.
DIPiDIP-5869N.
IntActiP07276. 1 interactor.
MINTiMINT-683829.

Structurei

Secondary structure

11031
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 10Combined sources5
Helixi11 – 13Combined sources3
Beta strandi15 – 18Combined sources4
Helixi19 – 22Combined sources4
Beta strandi26 – 30Combined sources5
Helixi34 – 41Combined sources8
Beta strandi45 – 49Combined sources5
Helixi53 – 67Combined sources15
Beta strandi71 – 76Combined sources6
Helixi82 – 96Combined sources15
Beta strandi667 – 669Combined sources3
Helixi769 – 781Combined sources13
Beta strandi786 – 788Combined sources3
Helixi793 – 802Combined sources10
Beta strandi807 – 810Combined sources4
Helixi815 – 818Combined sources4
Beta strandi823 – 827Combined sources5
Beta strandi830 – 839Combined sources10
Helixi840 – 847Combined sources8
Helixi851 – 861Combined sources11
Helixi874 – 884Combined sources11
Helixi887 – 899Combined sources13
Helixi901 – 904Combined sources4
Helixi909 – 920Combined sources12
Helixi932 – 939Combined sources8
Helixi957 – 968Combined sources12
Helixi972 – 982Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LOXNMR-B642-690[»]
4Q0RX-ray2.75A/B2-986[»]
4Q0WX-ray2.10A/B2-986[»]
4Q0ZX-ray2.40A/B/E/F2-986[»]
4Q10X-ray2.70A/B2-986[»]
ProteinModelPortaliP07276.
SMRiP07276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 95N-domainAdd BLAST95
Regioni756 – 884I-domainAdd BLAST129

Sequence similaritiesi

Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00640000091542.
HOGENOMiHOG000214817.
InParanoidiP07276.
KOiK10846.
OMAiAIDSSIW.
OrthoDBiEOG092C2ISI.

Family and domain databases

Gene3Di3.40.50.1010. 1 hit.
InterProiView protein in InterPro
IPR020045. 5-3_exonuclease_C.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR001044. XPG/Rad2_eukaryotes.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
PfamiView protein in Pfam
PF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
PRINTSiPR00853. XPGRADSUPER.
PR00066. XRODRMPGMNTG.
SMARTiView protein in SMART
SM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
SUPFAMiSSF47807. SSF47807. 2 hits.
SSF88723. SSF88723. 2 hits.
TIGRFAMsiTIGR00600. rad2. 1 hit.
PROSITEiView protein in PROSITE
PS00841. XPG_1. 1 hit.
PS00842. XPG_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P07276-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVHSFWDIA GPTARPVRLE SLEDKRMAVD ASIWIYQFLK AVRDQEGNAV 
        60         70         80         90        100
KNSHITGFFR RICKLLYFGI RPVFVFDGGV PVLKRETIRQ RKERRQGKRE 
       110        120        130        140        150
SAKSTARKLL ALQLQNGSND NVKNSTPSSG SSVQIFKPQD EWDLPDIPGF 
       160        170        180        190        200
KYDKEDARVN SNKTFEKLMN SINGDGLEDI DLDTINPASA EFEELPKATQ 
       210        220        230        240        250
YLILSSLRLK SRLRMGYSKE QLETIFPNSM DFSRFQIDMV KRRNFFTQKL 
       260        270        280        290        300
INTTGFQDGG ASKLNEEVIN RISGQKSKEY KLTKTNNGWI LGLGANDGSD 
       310        320        330        340        350
AQKAIVIDDK DAGALVKQLD SNAEDGDVLR WDDLEDNSLK IVRHESSNAT 
       360        370        380        390        400
TAPQKRSNRS EDEGCDSDEC EWEEVELKPK NVKFVEDFSL KAARLPYMGQ 
       410        420        430        440        450
SLNNAGSKSF LDKRHDQASP SKTTPTMRIS RISVEDDDED YLKQIEEIEM 
       460        470        480        490        500
MEAVQLSKME KKPEADDKSK IAKPVTSKGT EARPPIVQYG LLGAQPDSKQ 
       510        520        530        540        550
PYHVTNLNSK SESVIKRTSK TVLSEFRPPS QQEDKGAILT EGEQNLNFIS 
       560        570        580        590        600
HKIPQFDFNN ENSLLFQKNT ESNVSQEATK EKSPIPEMPS WFSSTASQQL 
       610        620        630        640        650
YNPYNTTNFV EDKNVRNEQE SGAETTNKGS SYELLTGLNA TEILERESEK 
       660        670        680        690        700
ESSNDENKDD DLEVLSEELF EDVPTKSQIS KEAEDNDSRK VESINKEHRK 
       710        720        730        740        750
PLIFDYDFSE DEEDNIVENM IKEQEEFDTF KNTTLSTSAE RNVAENAFVE 
       760        770        780        790        800
DELFEQQMKD KRDSDEVTMD MIKEVQELLS RFGIPYITAP MEAEAQCAEL 
       810        820        830        840        850
LQLNLVDGII TDDSDVFLFG GTKIYKNMFH EKNYVEFYDA ESILKLLGLD 
       860        870        880        890        900
RKNMIELAQL LGSDYTNGLK GMGPVSSIEV IAEFGNLKNF KDWYNNGQFD 
       910        920        930        940        950
KRKQETENKF EKDLRKKLVN NEIILDDDFP SVMVYDAYMR PEVDHDTTPF 
       960        970        980        990       1000
VWGVPDLDML RSFMKTQLGW PHEKSDEILI PLIRDVNKRK KKGKQKRINE 
      1010       1020       1030 
FFPREYISGD KKLNTSKRIS TATGKLKKRK M
Length:1,031
Mass (Da):117,838
Last modified:November 1, 1991 - v2
Checksum:i682D4AECFBD7F0F3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti782F → L in AAT92876 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10275 Genomic DNA. Translation: AAA66928.1.
Y07777 Genomic DNA. Translation: CAA69080.1.
Z73043 Genomic DNA. Translation: CAA97287.1.
BK006941 Genomic DNA. Translation: DAA08349.1.
AY692857 Genomic DNA. Translation: AAT92876.1.
PIRiA29839.
RefSeqiNP_011774.1. NM_001181387.1.

Genome annotation databases

EnsemblFungiiYGR258C; YGR258C; YGR258C.
GeneIDi853174.
KEGGisce:YGR258C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10275 Genomic DNA. Translation: AAA66928.1.
Y07777 Genomic DNA. Translation: CAA69080.1.
Z73043 Genomic DNA. Translation: CAA97287.1.
BK006941 Genomic DNA. Translation: DAA08349.1.
AY692857 Genomic DNA. Translation: AAT92876.1.
PIRiA29839.
RefSeqiNP_011774.1. NM_001181387.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LOXNMR-B642-690[»]
4Q0RX-ray2.75A/B2-986[»]
4Q0WX-ray2.10A/B2-986[»]
4Q0ZX-ray2.40A/B/E/F2-986[»]
4Q10X-ray2.70A/B2-986[»]
ProteinModelPortaliP07276.
SMRiP07276.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33510. 65 interactors.
DIPiDIP-5869N.
IntActiP07276. 1 interactor.
MINTiMINT-683829.

PTM databases

iPTMnetiP07276.

Proteomic databases

MaxQBiP07276.
PRIDEiP07276.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR258C; YGR258C; YGR258C.
GeneIDi853174.
KEGGisce:YGR258C.

Organism-specific databases

EuPathDBiFungiDB:YGR258C.
SGDiS000003490. RAD2.

Phylogenomic databases

GeneTreeiENSGT00640000091542.
HOGENOMiHOG000214817.
InParanoidiP07276.
KOiK10846.
OMAiAIDSSIW.
OrthoDBiEOG092C2ISI.

Enzyme and pathway databases

BioCyciYEAST:G3O-30928-MONOMER.
ReactomeiR-SCE-5696395. Formation of Incision Complex in GG-NER.
R-SCE-5696400. Dual Incision in GG-NER.
R-SCE-6782135. Dual incision in TC-NER.

Miscellaneous databases

PROiPR:P07276.

Family and domain databases

Gene3Di3.40.50.1010. 1 hit.
InterProiView protein in InterPro
IPR020045. 5-3_exonuclease_C.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR001044. XPG/Rad2_eukaryotes.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
PfamiView protein in Pfam
PF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
PRINTSiPR00853. XPGRADSUPER.
PR00066. XRODRMPGMNTG.
SMARTiView protein in SMART
SM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
SUPFAMiSSF47807. SSF47807. 2 hits.
SSF88723. SSF88723. 2 hits.
TIGRFAMsiTIGR00600. rad2. 1 hit.
PROSITEiView protein in PROSITE
PS00841. XPG_1. 1 hit.
PS00842. XPG_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRAD2_YEAST
AccessioniPrimary (citable) accession number: P07276
Secondary accession number(s): D6VV38, E9P8X9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1991
Last modified: June 7, 2017
This is version 172 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.