RAD2

Functionⁱ

Single-stranded DNA endonuclease involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Essential for the incision step of excision-repair.1 Publication

Miscellaneous

Present with 846 molecules/cell in log phase SD medium.1 Publication

Cofactorⁱ

Mg²⁺By similarityNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	30	Magnesium 1By similarity	1
Metal bindingⁱ	77	Magnesium 1By similarity	1
Metal bindingⁱ	792	Magnesium 1By similarity	1
Metal bindingⁱ	794	Magnesium 1By similarity	1
Metal bindingⁱ	813	Magnesium 2By similarity	1
Metal bindingⁱ	815	Magnesium 2By similarity	1
Metal bindingⁱ	864	Magnesium 2By similarity	1

GO - Molecular functionⁱ

metal ion binding Source: UniProtKB-KW
single-stranded DNA binding Source: GO_Central
single-stranded DNA endodeoxyribonuclease activity
Source: SGD
Inferred from direct assayⁱ
- 8247134

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Endonuclease, Hydrolase, Nuclease
Biological process	DNA damage, DNA repair
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-30928-MONOMER
Reactomeⁱ	R-SCE-5696395 Formation of Incision Complex in GG-NER R-SCE-5696400 Dual Incision in GG-NER R-SCE-6782135 Dual incision in TC-NER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: DNA repair protein RAD2 (EC:3.1.-.-)
Gene namesⁱ	Name:RAD2 Ordered Locus Names:YGR258C
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome VII

Organism-specific databases

EuPathDBⁱ	FungiDB:YGR258C
Saccharomyces Genome Database More...SGDⁱ	S000003490 RAD2

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000154035	1 – 1031	DNA repair protein RAD2Add BLAST		1031

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	118	PhosphoserineCombined sources	1
Modified residueⁱ	367	PhosphoserineCombined sources	1
Modified residueⁱ	583	PhosphoserineCombined sources	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P07276
PaxDbⁱ	P07276
PRIDEⁱ	P07276

PTM databases

iPTMnetⁱ	P07276

iPTMnetⁱ

P07276

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
HSP60	P19882	2	EBI-14757,EBI-8586
SEN1	Q00416	3	EBI-14757,EBI-16945

Show more details

Protein-protein interaction databases

BioGridⁱ	33510, 73 interactors
Database of interacting proteins More...DIPⁱ	DIP-5869N
ELMⁱ	P07276
IntActⁱ	P07276, 7 interactors
STRINGⁱ	4932.YGR258C

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	667 – 669	Combined sources	3
Helixⁱ	769 – 781	Combined sources	13
Beta strandⁱ	786 – 788	Combined sources	3
Helixⁱ	793 – 802	Combined sources	10
Beta strandⁱ	807 – 810	Combined sources	4
Helixⁱ	815 – 818	Combined sources	4
Beta strandⁱ	823 – 827	Combined sources	5
Beta strandⁱ	830 – 839	Combined sources	10
Helixⁱ	840 – 847	Combined sources	8
Helixⁱ	851 – 861	Combined sources	11
Helixⁱ	874 – 884	Combined sources	11
Helixⁱ	887 – 899	Combined sources	13
Helixⁱ	901 – 904	Combined sources	4
Helixⁱ	909 – 920	Combined sources	12
Helixⁱ	932 – 939	Combined sources	8
Helixⁱ	957 – 968	Combined sources	12
Helixⁱ	972 – 982	Combined sources	11

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2LOX	NMR	-	B	642-690	[»]
	4Q0R	X-ray	2.75	A/B	1-111	[»]
				A/B	732-986	[»]
	4Q0W	X-ray	2.10	A/B	2-111	[»]
				A/B	732-986	[»]
	4Q0Z	X-ray	2.40	A/B/E/F	2-111	[»]
				A/B/E/F	732-986	[»]
	4Q10	X-ray	2.70	A/B	2-111	[»]
				A/B	732-986	[»]
ProteinModelPortalⁱ	P07276
SMRⁱ	P07276
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	1 – 95	N-domainAdd BLAST		95
Regionⁱ	756 – 884	I-domainAdd BLAST		129

Sequence similaritiesⁱ

Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.Curated

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00640000091542
HOGENOMⁱ	HOG000214817
InParanoidⁱ	P07276
KEGG Orthology (KO) More...KOⁱ	K10846
OMAⁱ	AIDSSIW
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C2ISI

Family and domain databases

InterProⁱ	View protein in InterPro IPR036279 5-3_exonuclease_C_sf IPR008918 HhH2 IPR029060 PIN-like_dom_sf IPR006086 XPG-I_dom IPR006084 XPG/Rad2 IPR001044 XPG/Rad2_eukaryotes IPR019974 XPG_CS IPR006085 XPG_DNA_repair_N
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00867 XPG_I, 1 hit PF00752 XPG_N, 1 hit
PRINTSⁱ	PR00853 XPGRADSUPER PR00066 XRODRMPGMNTG
SMARTⁱ	View protein in SMART SM00279 HhH2, 1 hit SM00484 XPGI, 1 hit SM00485 XPGN, 1 hit
SUPFAMⁱ	SSF47807 SSF47807, 2 hits SSF88723 SSF88723, 2 hits
TIGRFAMsⁱ	TIGR00600 rad2, 1 hit
PROSITEⁱ	View protein in PROSITE PS00841 XPG_1, 1 hit PS00842 XPG_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P07276-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MGVHSFWDIA GPTARPVRLE SLEDKRMAVD ASIWIYQFLK AVRDQEGNAV 
        60         70         80         90        100
KNSHITGFFR RICKLLYFGI RPVFVFDGGV PVLKRETIRQ RKERRQGKRE 
       110        120        130        140        150
SAKSTARKLL ALQLQNGSND NVKNSTPSSG SSVQIFKPQD EWDLPDIPGF 
       160        170        180        190        200
KYDKEDARVN SNKTFEKLMN SINGDGLEDI DLDTINPASA EFEELPKATQ 
       210        220        230        240        250
YLILSSLRLK SRLRMGYSKE QLETIFPNSM DFSRFQIDMV KRRNFFTQKL 
       260        270        280        290        300
INTTGFQDGG ASKLNEEVIN RISGQKSKEY KLTKTNNGWI LGLGANDGSD 
       310        320        330        340        350
AQKAIVIDDK DAGALVKQLD SNAEDGDVLR WDDLEDNSLK IVRHESSNAT 
       360        370        380        390        400
TAPQKRSNRS EDEGCDSDEC EWEEVELKPK NVKFVEDFSL KAARLPYMGQ 
       410        420        430        440        450
SLNNAGSKSF LDKRHDQASP SKTTPTMRIS RISVEDDDED YLKQIEEIEM 
       460        470        480        490        500
MEAVQLSKME KKPEADDKSK IAKPVTSKGT EARPPIVQYG LLGAQPDSKQ 
       510        520        530        540        550
PYHVTNLNSK SESVIKRTSK TVLSEFRPPS QQEDKGAILT EGEQNLNFIS 
       560        570        580        590        600
HKIPQFDFNN ENSLLFQKNT ESNVSQEATK EKSPIPEMPS WFSSTASQQL 
       610        620        630        640        650
YNPYNTTNFV EDKNVRNEQE SGAETTNKGS SYELLTGLNA TEILERESEK 
       660        670        680        690        700
ESSNDENKDD DLEVLSEELF EDVPTKSQIS KEAEDNDSRK VESINKEHRK 
       710        720        730        740        750
PLIFDYDFSE DEEDNIVENM IKEQEEFDTF KNTTLSTSAE RNVAENAFVE 
       760        770        780        790        800
DELFEQQMKD KRDSDEVTMD MIKEVQELLS RFGIPYITAP MEAEAQCAEL 
       810        820        830        840        850
LQLNLVDGII TDDSDVFLFG GTKIYKNMFH EKNYVEFYDA ESILKLLGLD 
       860        870        880        890        900
RKNMIELAQL LGSDYTNGLK GMGPVSSIEV IAEFGNLKNF KDWYNNGQFD 
       910        920        930        940        950
KRKQETENKF EKDLRKKLVN NEIILDDDFP SVMVYDAYMR PEVDHDTTPF 
       960        970        980        990       1000
VWGVPDLDML RSFMKTQLGW PHEKSDEILI PLIRDVNKRK KKGKQKRINE 
      1010       1020       1030 
FFPREYISGD KKLNTSKRIS TATGKLKKRK M

Length:1,031

Mass (Da):117,838

Last modified:November 1, 1991 - v2

Checksum:ⁱ682D4AECFBD7F0F3

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	782	F → L in AAT92876 (PubMed:17322287).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M10275 Genomic DNA Translation: AAA66928.1 Y07777 Genomic DNA Translation: CAA69080.1 Z73043 Genomic DNA Translation: CAA97287.1 BK006941 Genomic DNA Translation: DAA08349.1 AY692857 Genomic DNA Translation: AAT92876.1
PIRⁱ	A29839
NCBI Reference Sequences More...RefSeqⁱ	NP_011774.1, NM_001181387.1

Genome annotation databases

EnsemblFungiⁱ	YGR258C; YGR258C; YGR258C
GeneIDⁱ	853174
KEGGⁱ	sce:YGR258C

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
P07276	Rad2p		YEASC		1031
K7_Rad2p		YEASK		1031
Rad2p		YEAS8		1031
Rad2p		SACCK		1031
RAD2p Single-stranded DNA endonuclease		Saccharomyces sp. 'boulardii'		1031
+4

Protein	Similar proteins		Species	Score	Length
P07276	Rad2p		YEASC		1031
RAD2p Single-stranded DNA endonuclease		Saccharomyces sp. 'boulardii'		1031
Rad2p		YEAS2		1031
K7_Rad2p		YEASK		1031
Rad2p		SACCK		1031
+6

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M10275 Genomic DNA Translation: AAA66928.1 Y07777 Genomic DNA Translation: CAA69080.1 Z73043 Genomic DNA Translation: CAA97287.1 BK006941 Genomic DNA Translation: DAA08349.1 AY692857 Genomic DNA Translation: AAT92876.1
PIRⁱ	A29839
NCBI Reference Sequences More...RefSeqⁱ	NP_011774.1, NM_001181387.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2LOX	NMR	-	B	642-690	[»]
	4Q0R	X-ray	2.75	A/B	1-111	[»]
				A/B	732-986	[»]
	4Q0W	X-ray	2.10	A/B	2-111	[»]
				A/B	732-986	[»]
	4Q0Z	X-ray	2.40	A/B/E/F	2-111	[»]
				A/B/E/F	732-986	[»]
	4Q10	X-ray	2.70	A/B	2-111	[»]
				A/B	732-986	[»]
ProteinModelPortalⁱ	P07276
SMRⁱ	P07276
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	33510, 73 interactors
Database of interacting proteins More...DIPⁱ	DIP-5869N
ELMⁱ	P07276
IntActⁱ	P07276, 7 interactors
STRINGⁱ	4932.YGR258C

PTM databases

iPTMnetⁱ	P07276

iPTMnetⁱ

P07276

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P07276
PaxDbⁱ	P07276
PRIDEⁱ	P07276

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YGR258C; YGR258C; YGR258C
GeneIDⁱ	853174
KEGGⁱ	sce:YGR258C

Organism-specific databases

EuPathDBⁱ	FungiDB:YGR258C
Saccharomyces Genome Database More...SGDⁱ	S000003490 RAD2

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00640000091542
HOGENOMⁱ	HOG000214817
InParanoidⁱ	P07276
KEGG Orthology (KO) More...KOⁱ	K10846
OMAⁱ	AIDSSIW
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C2ISI

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-30928-MONOMER
Reactomeⁱ	R-SCE-5696395 Formation of Incision Complex in GG-NER R-SCE-5696400 Dual Incision in GG-NER R-SCE-6782135 Dual incision in TC-NER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P07276

PROⁱ

PR:P07276

Family and domain databases

InterProⁱ	View protein in InterPro IPR036279 5-3_exonuclease_C_sf IPR008918 HhH2 IPR029060 PIN-like_dom_sf IPR006086 XPG-I_dom IPR006084 XPG/Rad2 IPR001044 XPG/Rad2_eukaryotes IPR019974 XPG_CS IPR006085 XPG_DNA_repair_N
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00867 XPG_I, 1 hit PF00752 XPG_N, 1 hit
PRINTSⁱ	PR00853 XPGRADSUPER PR00066 XRODRMPGMNTG
SMARTⁱ	View protein in SMART SM00279 HhH2, 1 hit SM00484 XPGI, 1 hit SM00485 XPGN, 1 hit
SUPFAMⁱ	SSF47807 SSF47807, 2 hits SSF88723 SSF88723, 2 hits
TIGRFAMsⁱ	TIGR00600 rad2, 1 hit
PROSITEⁱ	View protein in PROSITE PS00841 XPG_1, 1 hit PS00842 XPG_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RAD2_YEAST
Accessionⁱ	P07276Primary (citable) accession number: P07276 Secondary accession number(s): D6VV38, E9P8X9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
	Last sequence update:	November 1, 1991
	Last modified:	May 23, 2018
	This is version 182 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

UniProtKB

UniParc

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UniRef

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Supporting data

UniProtKB - P07276 (RAD2_YEAST)

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DNA repair protein RAD2

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ