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Protein

DNA repair protein RAD2

Gene

RAD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Single-stranded DNA endonuclease involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Essential for the incision step of excision-repair.1 Publication

Miscellaneous

Present with 846 molecules/cell in log phase SD medium.1 Publication

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi30Magnesium 1By similarity1
Metal bindingi77Magnesium 1By similarity1
Metal bindingi792Magnesium 1By similarity1
Metal bindingi794Magnesium 1By similarity1
Metal bindingi813Magnesium 2By similarity1
Metal bindingi815Magnesium 2By similarity1
Metal bindingi864Magnesium 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • nucleotide-excision repair, DNA incision, 3'-to lesion Source: SGD
  • transcription by RNA polymerase II Source: SGD

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30928-MONOMER
ReactomeiR-SCE-5696395 Formation of Incision Complex in GG-NER
R-SCE-5696400 Dual Incision in GG-NER
R-SCE-6782135 Dual incision in TC-NER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD2 (EC:3.1.-.-)
Gene namesi
Name:RAD2
Ordered Locus Names:YGR258C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR258C
SGDiS000003490 RAD2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001540351 – 1031DNA repair protein RAD2Add BLAST1031

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei118PhosphoserineCombined sources1
Modified residuei367PhosphoserineCombined sources1
Modified residuei583PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07276
PaxDbiP07276
PRIDEiP07276

PTM databases

iPTMnetiP07276

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33510, 73 interactors
DIPiDIP-5869N
ELMiP07276
IntActiP07276, 7 interactors
STRINGi4932.YGR258C

Structurei

Secondary structure

11031
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi667 – 669Combined sources3
Helixi769 – 781Combined sources13
Beta strandi786 – 788Combined sources3
Helixi793 – 802Combined sources10
Beta strandi807 – 810Combined sources4
Helixi815 – 818Combined sources4
Beta strandi823 – 827Combined sources5
Beta strandi830 – 839Combined sources10
Helixi840 – 847Combined sources8
Helixi851 – 861Combined sources11
Helixi874 – 884Combined sources11
Helixi887 – 899Combined sources13
Helixi901 – 904Combined sources4
Helixi909 – 920Combined sources12
Helixi932 – 939Combined sources8
Helixi957 – 968Combined sources12
Helixi972 – 982Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LOXNMR-B642-690[»]
4Q0RX-ray2.75A/B1-111[»]
A/B732-986[»]
4Q0WX-ray2.10A/B2-111[»]
A/B732-986[»]
4Q0ZX-ray2.40A/B/E/F2-111[»]
A/B/E/F732-986[»]
4Q10X-ray2.70A/B2-111[»]
A/B732-986[»]
ProteinModelPortaliP07276
SMRiP07276
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 95N-domainAdd BLAST95
Regioni756 – 884I-domainAdd BLAST129

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00640000091542
HOGENOMiHOG000214817
InParanoidiP07276
KOiK10846
OMAiAIDSSIW
OrthoDBiEOG092C2ISI

Family and domain databases

InterProiView protein in InterPro
IPR036279 5-3_exonuclease_C_sf
IPR008918 HhH2
IPR029060 PIN-like_dom_sf
IPR006086 XPG-I_dom
IPR006084 XPG/Rad2
IPR001044 XPG/Rad2_eukaryotes
IPR019974 XPG_CS
IPR006085 XPG_DNA_repair_N
PfamiView protein in Pfam
PF00867 XPG_I, 1 hit
PF00752 XPG_N, 1 hit
PRINTSiPR00853 XPGRADSUPER
PR00066 XRODRMPGMNTG
SMARTiView protein in SMART
SM00279 HhH2, 1 hit
SM00484 XPGI, 1 hit
SM00485 XPGN, 1 hit
SUPFAMiSSF47807 SSF47807, 2 hits
SSF88723 SSF88723, 2 hits
TIGRFAMsiTIGR00600 rad2, 1 hit
PROSITEiView protein in PROSITE
PS00841 XPG_1, 1 hit
PS00842 XPG_2, 1 hit

Sequencei

Sequence statusi: Complete.

P07276-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVHSFWDIA GPTARPVRLE SLEDKRMAVD ASIWIYQFLK AVRDQEGNAV
60 70 80 90 100
KNSHITGFFR RICKLLYFGI RPVFVFDGGV PVLKRETIRQ RKERRQGKRE
110 120 130 140 150
SAKSTARKLL ALQLQNGSND NVKNSTPSSG SSVQIFKPQD EWDLPDIPGF
160 170 180 190 200
KYDKEDARVN SNKTFEKLMN SINGDGLEDI DLDTINPASA EFEELPKATQ
210 220 230 240 250
YLILSSLRLK SRLRMGYSKE QLETIFPNSM DFSRFQIDMV KRRNFFTQKL
260 270 280 290 300
INTTGFQDGG ASKLNEEVIN RISGQKSKEY KLTKTNNGWI LGLGANDGSD
310 320 330 340 350
AQKAIVIDDK DAGALVKQLD SNAEDGDVLR WDDLEDNSLK IVRHESSNAT
360 370 380 390 400
TAPQKRSNRS EDEGCDSDEC EWEEVELKPK NVKFVEDFSL KAARLPYMGQ
410 420 430 440 450
SLNNAGSKSF LDKRHDQASP SKTTPTMRIS RISVEDDDED YLKQIEEIEM
460 470 480 490 500
MEAVQLSKME KKPEADDKSK IAKPVTSKGT EARPPIVQYG LLGAQPDSKQ
510 520 530 540 550
PYHVTNLNSK SESVIKRTSK TVLSEFRPPS QQEDKGAILT EGEQNLNFIS
560 570 580 590 600
HKIPQFDFNN ENSLLFQKNT ESNVSQEATK EKSPIPEMPS WFSSTASQQL
610 620 630 640 650
YNPYNTTNFV EDKNVRNEQE SGAETTNKGS SYELLTGLNA TEILERESEK
660 670 680 690 700
ESSNDENKDD DLEVLSEELF EDVPTKSQIS KEAEDNDSRK VESINKEHRK
710 720 730 740 750
PLIFDYDFSE DEEDNIVENM IKEQEEFDTF KNTTLSTSAE RNVAENAFVE
760 770 780 790 800
DELFEQQMKD KRDSDEVTMD MIKEVQELLS RFGIPYITAP MEAEAQCAEL
810 820 830 840 850
LQLNLVDGII TDDSDVFLFG GTKIYKNMFH EKNYVEFYDA ESILKLLGLD
860 870 880 890 900
RKNMIELAQL LGSDYTNGLK GMGPVSSIEV IAEFGNLKNF KDWYNNGQFD
910 920 930 940 950
KRKQETENKF EKDLRKKLVN NEIILDDDFP SVMVYDAYMR PEVDHDTTPF
960 970 980 990 1000
VWGVPDLDML RSFMKTQLGW PHEKSDEILI PLIRDVNKRK KKGKQKRINE
1010 1020 1030
FFPREYISGD KKLNTSKRIS TATGKLKKRK M
Length:1,031
Mass (Da):117,838
Last modified:November 1, 1991 - v2
Checksum:i682D4AECFBD7F0F3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti782F → L in AAT92876 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10275 Genomic DNA Translation: AAA66928.1
Y07777 Genomic DNA Translation: CAA69080.1
Z73043 Genomic DNA Translation: CAA97287.1
BK006941 Genomic DNA Translation: DAA08349.1
AY692857 Genomic DNA Translation: AAT92876.1
PIRiA29839
RefSeqiNP_011774.1, NM_001181387.1

Genome annotation databases

EnsemblFungiiYGR258C; YGR258C; YGR258C
GeneIDi853174
KEGGisce:YGR258C

Similar proteinsi

Entry informationi

Entry nameiRAD2_YEAST
AccessioniPrimary (citable) accession number: P07276
Secondary accession number(s): D6VV38, E9P8X9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1991
Last modified: May 23, 2018
This is version 182 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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