UniProtKB - P07276 (RAD2_YEAST)
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Protein
DNA repair protein RAD2
Gene
RAD2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Single-stranded DNA endonuclease involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Essential for the incision step of excision-repair.1 Publication
Miscellaneous
Present with 846 molecules/cell in log phase SD medium.1 Publication
Cofactori
Mg2+By similarityNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 30 | Magnesium 1By similarity | 1 | |
Metal bindingi | 77 | Magnesium 1By similarity | 1 | |
Metal bindingi | 792 | Magnesium 1By similarity | 1 | |
Metal bindingi | 794 | Magnesium 1By similarity | 1 | |
Metal bindingi | 813 | Magnesium 2By similarity | 1 | |
Metal bindingi | 815 | Magnesium 2By similarity | 1 | |
Metal bindingi | 864 | Magnesium 2By similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- single-stranded DNA binding Source: GO_Central
- single-stranded DNA endodeoxyribonuclease activity Source: SGD
GO - Biological processi
- nucleotide-excision repair, DNA incision, 3'-to lesion Source: SGD
- transcription by RNA polymerase II Source: SGD
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease |
Biological process | DNA damage, DNA repair |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | YEAST:G3O-30928-MONOMER |
Reactomei | R-SCE-5696395 Formation of Incision Complex in GG-NER R-SCE-5696400 Dual Incision in GG-NER R-SCE-6782135 Dual incision in TC-NER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:RAD2 Ordered Locus Names:YGR258C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YGR258C |
SGDi | S000003490 RAD2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000154035 | 1 – 1031 | DNA repair protein RAD2Add BLAST | 1031 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 118 | PhosphoserineCombined sources | 1 | |
Modified residuei | 367 | PhosphoserineCombined sources | 1 | |
Modified residuei | 583 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P07276 |
PaxDbi | P07276 |
PRIDEi | P07276 |
PTM databases
iPTMneti | P07276 |
Interactioni
Binary interactionsi
Protein-protein interaction databases
BioGridi | 3351073 interactors. |
DIPi | DIP-5869N |
ELMi | P07276 |
IntActi | P07276 12 interactors. |
STRINGi | 4932.YGR258C |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Beta strandi | 667 – 669 | Combined sources | 3 | |
Helixi | 769 – 781 | Combined sources | 13 | |
Beta strandi | 786 – 788 | Combined sources | 3 | |
Helixi | 793 – 802 | Combined sources | 10 | |
Beta strandi | 807 – 810 | Combined sources | 4 | |
Helixi | 815 – 818 | Combined sources | 4 | |
Beta strandi | 823 – 827 | Combined sources | 5 | |
Beta strandi | 830 – 839 | Combined sources | 10 | |
Helixi | 840 – 847 | Combined sources | 8 | |
Helixi | 851 – 861 | Combined sources | 11 | |
Helixi | 874 – 884 | Combined sources | 11 | |
Helixi | 887 – 899 | Combined sources | 13 | |
Helixi | 901 – 904 | Combined sources | 4 | |
Helixi | 909 – 920 | Combined sources | 12 | |
Helixi | 932 – 939 | Combined sources | 8 | |
Helixi | 957 – 968 | Combined sources | 12 | |
Helixi | 972 – 982 | Combined sources | 11 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2LOX | NMR | - | B | 642-690 | [»] | |
4Q0R | X-ray | 2.75 | A/B | 1-111 | [»] | |
A/B | 732-986 | [»] | ||||
4Q0W | X-ray | 2.10 | A/B | 2-111 | [»] | |
A/B | 732-986 | [»] | ||||
4Q0Z | X-ray | 2.40 | A/B/E/F | 2-111 | [»] | |
A/B/E/F | 732-986 | [»] | ||||
4Q10 | X-ray | 2.70 | A/B | 2-111 | [»] | |
A/B | 732-986 | [»] | ||||
ProteinModelPortali | P07276 | |||||
SMRi | P07276 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 95 | N-domainAdd BLAST | 95 | |
Regioni | 756 – 884 | I-domainAdd BLAST | 129 |
Sequence similaritiesi
Phylogenomic databases
GeneTreei | ENSGT00640000091542 |
HOGENOMi | HOG000214817 |
InParanoidi | P07276 |
KOi | K10846 |
OMAi | AIDSSIW |
OrthoDBi | EOG092C2ISI |
Family and domain databases
InterProi | View protein in InterPro IPR036279 5-3_exonuclease_C_sf IPR008918 HhH2 IPR029060 PIN-like_dom_sf IPR006086 XPG-I_dom IPR006084 XPG/Rad2 IPR001044 XPG/Rad2_eukaryotes IPR019974 XPG_CS IPR006085 XPG_DNA_repair_N |
Pfami | View protein in Pfam PF00867 XPG_I, 1 hit PF00752 XPG_N, 1 hit |
PRINTSi | PR00853 XPGRADSUPER PR00066 XRODRMPGMNTG |
SMARTi | View protein in SMART SM00279 HhH2, 1 hit SM00484 XPGI, 1 hit SM00485 XPGN, 1 hit |
SUPFAMi | SSF47807 SSF47807, 2 hits SSF88723 SSF88723, 2 hits |
TIGRFAMsi | TIGR00600 rad2, 1 hit |
PROSITEi | View protein in PROSITE PS00841 XPG_1, 1 hit PS00842 XPG_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P07276-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGVHSFWDIA GPTARPVRLE SLEDKRMAVD ASIWIYQFLK AVRDQEGNAV
60 70 80 90 100
KNSHITGFFR RICKLLYFGI RPVFVFDGGV PVLKRETIRQ RKERRQGKRE
110 120 130 140 150
SAKSTARKLL ALQLQNGSND NVKNSTPSSG SSVQIFKPQD EWDLPDIPGF
160 170 180 190 200
KYDKEDARVN SNKTFEKLMN SINGDGLEDI DLDTINPASA EFEELPKATQ
210 220 230 240 250
YLILSSLRLK SRLRMGYSKE QLETIFPNSM DFSRFQIDMV KRRNFFTQKL
260 270 280 290 300
INTTGFQDGG ASKLNEEVIN RISGQKSKEY KLTKTNNGWI LGLGANDGSD
310 320 330 340 350
AQKAIVIDDK DAGALVKQLD SNAEDGDVLR WDDLEDNSLK IVRHESSNAT
360 370 380 390 400
TAPQKRSNRS EDEGCDSDEC EWEEVELKPK NVKFVEDFSL KAARLPYMGQ
410 420 430 440 450
SLNNAGSKSF LDKRHDQASP SKTTPTMRIS RISVEDDDED YLKQIEEIEM
460 470 480 490 500
MEAVQLSKME KKPEADDKSK IAKPVTSKGT EARPPIVQYG LLGAQPDSKQ
510 520 530 540 550
PYHVTNLNSK SESVIKRTSK TVLSEFRPPS QQEDKGAILT EGEQNLNFIS
560 570 580 590 600
HKIPQFDFNN ENSLLFQKNT ESNVSQEATK EKSPIPEMPS WFSSTASQQL
610 620 630 640 650
YNPYNTTNFV EDKNVRNEQE SGAETTNKGS SYELLTGLNA TEILERESEK
660 670 680 690 700
ESSNDENKDD DLEVLSEELF EDVPTKSQIS KEAEDNDSRK VESINKEHRK
710 720 730 740 750
PLIFDYDFSE DEEDNIVENM IKEQEEFDTF KNTTLSTSAE RNVAENAFVE
760 770 780 790 800
DELFEQQMKD KRDSDEVTMD MIKEVQELLS RFGIPYITAP MEAEAQCAEL
810 820 830 840 850
LQLNLVDGII TDDSDVFLFG GTKIYKNMFH EKNYVEFYDA ESILKLLGLD
860 870 880 890 900
RKNMIELAQL LGSDYTNGLK GMGPVSSIEV IAEFGNLKNF KDWYNNGQFD
910 920 930 940 950
KRKQETENKF EKDLRKKLVN NEIILDDDFP SVMVYDAYMR PEVDHDTTPF
960 970 980 990 1000
VWGVPDLDML RSFMKTQLGW PHEKSDEILI PLIRDVNKRK KKGKQKRINE
1010 1020 1030
FFPREYISGD KKLNTSKRIS TATGKLKKRK M
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 782 | F → L in AAT92876 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M10275 Genomic DNA Translation: AAA66928.1 Y07777 Genomic DNA Translation: CAA69080.1 Z73043 Genomic DNA Translation: CAA97287.1 BK006941 Genomic DNA Translation: DAA08349.1 AY692857 Genomic DNA Translation: AAT92876.1 |
PIRi | A29839 |
RefSeqi | NP_011774.1, NM_001181387.1 |
Genome annotation databases
EnsemblFungii | YGR258C; YGR258C; YGR258C |
GeneIDi | 853174 |
KEGGi | sce:YGR258C |
Similar proteinsi
Entry informationi
Entry namei | RAD2_YEAST | |
Accessioni | P07276Primary (citable) accession number: P07276 Secondary accession number(s): D6VV38, E9P8X9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | November 1, 1991 | |
Last modified: | March 28, 2018 | |
This is version 181 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |