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P07276

- RAD2_YEAST

UniProt

P07276 - RAD2_YEAST

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Protein

DNA repair protein RAD2

Gene

RAD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Single-stranded DNA endonuclease involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Essential for the incision step of excision-repair.1 Publication

Cofactori

Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi30 – 301Magnesium 1By similarity
Metal bindingi77 – 771Magnesium 1By similarity
Metal bindingi792 – 7921Magnesium 1By similarity
Metal bindingi794 – 7941Magnesium 1By similarity
Metal bindingi813 – 8131Magnesium 2By similarity
Metal bindingi815 – 8151Magnesium 2By similarity
Metal bindingi864 – 8641Magnesium 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. single-stranded DNA binding Source: InterPro
  3. single-stranded DNA endodeoxyribonuclease activity Source: SGD

GO - Biological processi

  1. DNA catabolic process, endonucleolytic Source: GOC
  2. nucleotide-excision repair, DNA incision, 3'-to lesion Source: SGD
  3. transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30928-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD2 (EC:3.1.-.-)
Gene namesi
Name:RAD2
Ordered Locus Names:YGR258C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR258c.
SGDiS000003490. RAD2.

Subcellular locationi

GO - Cellular componenti

  1. nucleotide-excision repair factor 3 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10311031DNA repair protein RAD2PRO_0000154035Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei118 – 1181Phosphoserine1 Publication
Modified residuei367 – 3671Phosphoserine1 Publication
Modified residuei583 – 5831Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07276.
PaxDbiP07276.
PeptideAtlasiP07276.
PRIDEiP07276.

Expressioni

Gene expression databases

GenevestigatoriP07276.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SEN1Q004163EBI-14757,EBI-16945

Protein-protein interaction databases

BioGridi33510. 45 interactions.
DIPiDIP-5869N.
IntActiP07276. 1 interaction.
MINTiMINT-683829.
STRINGi4932.YGR258C.

Structurei

Secondary structure

1
1031
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi667 – 6693

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LOXNMR-B642-690[»]
4Q0RX-ray2.75A/B2-986[»]
4Q0WX-ray2.10A/B2-986[»]
4Q0ZX-ray2.40A/B/E/F2-986[»]
4Q10X-ray2.70A/B2-986[»]
ProteinModelPortaliP07276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9595N-domainAdd
BLAST
Regioni756 – 884129I-domainAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0258.
GeneTreeiENSGT00640000091542.
HOGENOMiHOG000214817.
InParanoidiP07276.
KOiK10846.
OMAiFQATMRD.
OrthoDBiEOG7QK0MK.

Family and domain databases

Gene3Di3.40.50.1010. 2 hits.
InterProiIPR020045. 5-3_exonuclease_C.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR001044. XPG/Rad2_eukaryotes.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PANTHERiPTHR11081:SF1. PTHR11081:SF1. 1 hit.
PfamiPF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
PRINTSiPR00066. XRODRMPGMNTG.
SMARTiSM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 2 hits.
SSF88723. SSF88723. 2 hits.
TIGRFAMsiTIGR00600. rad2. 1 hit.
PROSITEiPS00841. XPG_1. 1 hit.
PS00842. XPG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07276-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGVHSFWDIA GPTARPVRLE SLEDKRMAVD ASIWIYQFLK AVRDQEGNAV
60 70 80 90 100
KNSHITGFFR RICKLLYFGI RPVFVFDGGV PVLKRETIRQ RKERRQGKRE
110 120 130 140 150
SAKSTARKLL ALQLQNGSND NVKNSTPSSG SSVQIFKPQD EWDLPDIPGF
160 170 180 190 200
KYDKEDARVN SNKTFEKLMN SINGDGLEDI DLDTINPASA EFEELPKATQ
210 220 230 240 250
YLILSSLRLK SRLRMGYSKE QLETIFPNSM DFSRFQIDMV KRRNFFTQKL
260 270 280 290 300
INTTGFQDGG ASKLNEEVIN RISGQKSKEY KLTKTNNGWI LGLGANDGSD
310 320 330 340 350
AQKAIVIDDK DAGALVKQLD SNAEDGDVLR WDDLEDNSLK IVRHESSNAT
360 370 380 390 400
TAPQKRSNRS EDEGCDSDEC EWEEVELKPK NVKFVEDFSL KAARLPYMGQ
410 420 430 440 450
SLNNAGSKSF LDKRHDQASP SKTTPTMRIS RISVEDDDED YLKQIEEIEM
460 470 480 490 500
MEAVQLSKME KKPEADDKSK IAKPVTSKGT EARPPIVQYG LLGAQPDSKQ
510 520 530 540 550
PYHVTNLNSK SESVIKRTSK TVLSEFRPPS QQEDKGAILT EGEQNLNFIS
560 570 580 590 600
HKIPQFDFNN ENSLLFQKNT ESNVSQEATK EKSPIPEMPS WFSSTASQQL
610 620 630 640 650
YNPYNTTNFV EDKNVRNEQE SGAETTNKGS SYELLTGLNA TEILERESEK
660 670 680 690 700
ESSNDENKDD DLEVLSEELF EDVPTKSQIS KEAEDNDSRK VESINKEHRK
710 720 730 740 750
PLIFDYDFSE DEEDNIVENM IKEQEEFDTF KNTTLSTSAE RNVAENAFVE
760 770 780 790 800
DELFEQQMKD KRDSDEVTMD MIKEVQELLS RFGIPYITAP MEAEAQCAEL
810 820 830 840 850
LQLNLVDGII TDDSDVFLFG GTKIYKNMFH EKNYVEFYDA ESILKLLGLD
860 870 880 890 900
RKNMIELAQL LGSDYTNGLK GMGPVSSIEV IAEFGNLKNF KDWYNNGQFD
910 920 930 940 950
KRKQETENKF EKDLRKKLVN NEIILDDDFP SVMVYDAYMR PEVDHDTTPF
960 970 980 990 1000
VWGVPDLDML RSFMKTQLGW PHEKSDEILI PLIRDVNKRK KKGKQKRINE
1010 1020 1030
FFPREYISGD KKLNTSKRIS TATGKLKKRK M
Length:1,031
Mass (Da):117,838
Last modified:November 1, 1991 - v2
Checksum:i682D4AECFBD7F0F3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti782 – 7821F → L in AAT92876. (PubMed:17322287)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10275 Genomic DNA. Translation: AAA66928.1.
Y07777 Genomic DNA. Translation: CAA69080.1.
Z73043 Genomic DNA. Translation: CAA97287.1.
BK006941 Genomic DNA. Translation: DAA08349.1.
AY692857 Genomic DNA. Translation: AAT92876.1.
PIRiA29839.
RefSeqiNP_011774.1. NM_001181387.1.

Genome annotation databases

EnsemblFungiiYGR258C; YGR258C; YGR258C.
GeneIDi853174.
KEGGisce:YGR258C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10275 Genomic DNA. Translation: AAA66928.1 .
Y07777 Genomic DNA. Translation: CAA69080.1 .
Z73043 Genomic DNA. Translation: CAA97287.1 .
BK006941 Genomic DNA. Translation: DAA08349.1 .
AY692857 Genomic DNA. Translation: AAT92876.1 .
PIRi A29839.
RefSeqi NP_011774.1. NM_001181387.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2LOX NMR - B 642-690 [» ]
4Q0R X-ray 2.75 A/B 2-986 [» ]
4Q0W X-ray 2.10 A/B 2-986 [» ]
4Q0Z X-ray 2.40 A/B/E/F 2-986 [» ]
4Q10 X-ray 2.70 A/B 2-986 [» ]
ProteinModelPortali P07276.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33510. 45 interactions.
DIPi DIP-5869N.
IntActi P07276. 1 interaction.
MINTi MINT-683829.
STRINGi 4932.YGR258C.

Proteomic databases

MaxQBi P07276.
PaxDbi P07276.
PeptideAtlasi P07276.
PRIDEi P07276.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR258C ; YGR258C ; YGR258C .
GeneIDi 853174.
KEGGi sce:YGR258C.

Organism-specific databases

CYGDi YGR258c.
SGDi S000003490. RAD2.

Phylogenomic databases

eggNOGi COG0258.
GeneTreei ENSGT00640000091542.
HOGENOMi HOG000214817.
InParanoidi P07276.
KOi K10846.
OMAi FQATMRD.
OrthoDBi EOG7QK0MK.

Enzyme and pathway databases

BioCyci YEAST:G3O-30928-MONOMER.

Miscellaneous databases

NextBioi 973302.
PROi P07276.

Gene expression databases

Genevestigatori P07276.

Family and domain databases

Gene3Di 3.40.50.1010. 2 hits.
InterProi IPR020045. 5-3_exonuclease_C.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR001044. XPG/Rad2_eukaryotes.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
[Graphical view ]
PANTHERi PTHR11081:SF1. PTHR11081:SF1. 1 hit.
Pfami PF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view ]
PRINTSi PR00066. XRODRMPGMNTG.
SMARTi SM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view ]
SUPFAMi SSF47807. SSF47807. 2 hits.
SSF88723. SSF88723. 2 hits.
TIGRFAMsi TIGR00600. rad2. 1 hit.
PROSITEi PS00841. XPG_1. 1 hit.
PS00842. XPG_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The RAD2 gene of Saccharomyces cerevisiae: nucleotide sequence and transcript mapping."
    Nicolet C.M., Chenevert J.M., Friedberg E.C.
    Gene 36:225-234(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence, transcript mapping, and regulation of the RAD2 gene of Saccharomyces cerevisiae."
    Madura K., Prakash S.
    J. Bacteriol. 166:914-923(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Analysis of an 11.6 kb region from the right arm of chromosome VII of Saccharomyces cerevisiae between the RAD2 and the MES1 genes reveals the presence of three new genes."
    Clemente M.L., Sartori G., Cardazzo B., Carignani G.
    Yeast 13:287-290(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-51.
    Strain: ATCC 96604 / S288c / FY1679.
  7. "Yeast excision repair gene RAD2 encodes a single-stranded DNA endonuclease."
    Habraken Y., Sung P., Prakash L., Prakash S.
    Nature 366:365-368(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-583, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-118 AND SER-367, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRAD2_YEAST
AccessioniPrimary (citable) accession number: P07276
Secondary accession number(s): D6VV38, E9P8X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1991
Last modified: October 29, 2014
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 846 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3