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Reviewed, UniProtKB/Swiss-Prot P07275 (PUT2_YEAST)

Last modified November 3, 2009. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
      Short name=P5C dehydrogenase
    EC=1.5.1.12
Gene names
Name: PUT2
Ordered Locus Names: YHR037W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length575 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H.

Pathway

Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.

Subcellular location

Mitochondrion inner membrane. Ref.4

Induction

By proline and is regulated by a common control element encoded by the PUT3 gene.

Miscellaneous

Present with 17200 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-14303,EBI-14303
SSA2P105921EBI-14303,EBI-8603

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 575Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrialPRO_0000007177

Regions

Nucleotide binding297 – 3026NAD By similarity

Sites

Active site3171Proton acceptor By similarity
Active site3511Nucleophile By similarity
Site2121Transition state stabilizer By similarity

Experimental info

Sequence conflict187 – 1882ES → SR in AAA34924. Ref.1
Sequence conflict2641P → L in AAA34924. Ref.1
Sequence conflict5411D → G in AAA34924. Ref.1
Sequence conflict5611N → S in AAA34924. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P07275-1 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 5A610D67985291B0

FASTA57564,435
        10         20         30         40         50         60 
MLSARCLKSI YFKRSFSQLG HIKPPKHIRN EPVKPFRNID LKDWDLLRAS LMKFKSSSLE 

        70         80         90        100        110        120 
VPLVINGERI YDNNERALFP QTNPANHQQV LANVTQATEK DVMNAVKAAK DAKKDWYNLP 

       130        140        150        160        170        180 
FYDRSAIFLK AADLISTKYR YDMLAATMLG QGKNVYQAEI DCITELSDFF RYYVKYASDL 

       190        200        210        220        230        240 
YAQQPVESAD GTWNKAEYRP LEGFVYAVSP FNFTAIAANL IGAPALMGNT VVWKPSQTAA 

       250        260        270        280        290        300 
LSNYLLMTVL EEAGLPKGVI NFIPGDPVQV TDQVLADKDF GALHFTGSTN VFKSLYGKIQ 

       310        320        330        340        350        360 
SGVVEGKYRD YPRIIGETGG KNFHLVHPSA NISHAVLSTI RGTFEFQGQK CSAASRLYLP 

       370        380        390        400        410        420 
ESKSEEFLSD MFGILQSQNV VPMNTSASPI SGGNLRGFMG PVIHEQSFDK LVKVIEDAKK 

       430        440        450        460        470        480 
DPELEILYGG QYDKSQGWFV GPTVIKAKRP DHPYMSTEFF GPILTVYEYP DTEFNEICDI 

       490        500        510        520        530        540 
IDNTSQYALT GAIFAKDRKA IEYADEKLKF SAGNFYINDK CTGAVVSQQW FGGARMSGTD 

       550        560        570 
DKAGGPNILS RFVSIRNTKE NFYELTDFKY PSNYE 

« Hide

References

« Hide 'large scale' references
[1]"Primary structure of the nuclear PUT2 gene involved in the mitochondrial pathway for proline utilization in Saccharomyces cerevisiae."
Krzywicki K.A., Brandriss M.C.
Mol. Cell. Biol. 4:2837-2842(1984) [PubMed: 6098824] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"A regulatory region responsible for proline-specific induction of the yeast PUT2 gene is adjacent to its TATA box."
Siddiqui A.H., Brandriss M.C.
Mol. Cell. Biol. 8:4634-4641(1988) [PubMed: 3062363] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3.
[4]"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
Biochemistry 40:9758-9769(2001) [PubMed: 11502169] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

M10029 Genomic DNA. Translation: AAA34924.1.
M22785 Genomic DNA. No translation available.
U00062 Genomic DNA. Translation: AAB68907.1.
PIRRDBYC. S46738.
RefSeqNP_011902.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActP07275. 3 interactions.
STRINGP07275.

Proteomic databases

PeptideAtlasP07275.

Genome annotation databases

EnsemblYHR037W; YHR037W; YHR037W; Saccharomyces cerevisiae. [Genome view]
GeneID856432.
GenomeReviewsGene locus YHR037W in contig U00093_GR.
KEGGsce:YHR037W.
NMPDRfig|4932.3.peg.3051.

Organism-specific databases

CYGDYHR037w.
SGDS000001079. PUT2.

Phylogenomic databases

HOGENOMP07275.
OMARYAAGNY.

Enzyme and pathway databases

BioCycMetaCyc:MON-646.
BRENDA1.5.1.12. 250.

Gene expression databases

ArrayExpressP07275.
GenevestigatorP07275.
GermOnlineYHR037W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH.
IPR005931. d-1-pyrroline-5-COlate_DH-1.
[Graphical view]
Gene3DG3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHERPTHR11699. Aldehyde_dehyd. 1 hit.
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
TIGRFAMsTIGR01236. D1pyr5carbox1. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio982024.

Entry information

Entry namePUT2_YEAST
AccessionPrimary (citable) accession number: P07275
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1995
Last modified: November 3, 2009
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents