Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P07275 (PUT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 136. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
Short name=P5C dehydrogenase
EC=1.5.1.12

Gene names

Name:	PUT2
Ordered Locus Names:	YHR037W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)⁺ + 2 H₂O = L-glutamate + NAD(P)H.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.
Subcellular location	Mitochondrion inner membrane Ref.5.
Induction	By proline and is regulated by a common control element encoded by the PUT3 gene.
Miscellaneous	Present with 17200 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Proline metabolism
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamate biosynthetic process Inferred from direct assay PubMed 4145181. Source: SGD proline biosynthetic process Inferred from electronic annotation. Source: InterPro proline catabolic process to glutamate Inferred from mutant phenotype PubMed 387737. Source: SGD
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial matrix Inferred from direct assay PubMed 3025596. Source: SGD
Molecular_function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from direct assay PubMed 4145181. Source: SGD oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Mitochondrion Potential

Chain

? – 575

Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial

PRO_0000007177

Regions

Nucleotide binding

297 – 302

NAD By similarity

Sites

Active site

317

Proton acceptor By similarity

Active site

351

Nucleophile By similarity

Site

212

Transition state stabilizer By similarity

Experimental info

Sequence conflict

187 – 188

ES → SR in AAA34924. Ref.1

Sequence conflict

264

P → L in AAA34924. Ref.1

Sequence conflict

541

D → G in AAA34924. Ref.1

Sequence conflict

561

N → S in AAA34924. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P07275 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 5A610D67985291B0
Show »

FASTA

575

64,435

        10         20         30         40         50         60 
MLSARCLKSI YFKRSFSQLG HIKPPKHIRN EPVKPFRNID LKDWDLLRAS LMKFKSSSLE 

        70         80         90        100        110        120 
VPLVINGERI YDNNERALFP QTNPANHQQV LANVTQATEK DVMNAVKAAK DAKKDWYNLP 

       130        140        150        160        170        180 
FYDRSAIFLK AADLISTKYR YDMLAATMLG QGKNVYQAEI DCITELSDFF RYYVKYASDL 

       190        200        210        220        230        240 
YAQQPVESAD GTWNKAEYRP LEGFVYAVSP FNFTAIAANL IGAPALMGNT VVWKPSQTAA 

       250        260        270        280        290        300 
LSNYLLMTVL EEAGLPKGVI NFIPGDPVQV TDQVLADKDF GALHFTGSTN VFKSLYGKIQ 

       310        320        330        340        350        360 
SGVVEGKYRD YPRIIGETGG KNFHLVHPSA NISHAVLSTI RGTFEFQGQK CSAASRLYLP 

       370        380        390        400        410        420 
ESKSEEFLSD MFGILQSQNV VPMNTSASPI SGGNLRGFMG PVIHEQSFDK LVKVIEDAKK 

       430        440        450        460        470        480 
DPELEILYGG QYDKSQGWFV GPTVIKAKRP DHPYMSTEFF GPILTVYEYP DTEFNEICDI 

       490        500        510        520        530        540 
IDNTSQYALT GAIFAKDRKA IEYADEKLKF SAGNFYINDK CTGAVVSQQW FGGARMSGTD 

       550        560        570 
DKAGGPNILS RFVSIRNTKE NFYELTDFKY PSNYE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of the nuclear PUT2 gene involved in the mitochondrial pathway for proline utilization in Saccharomyces cerevisiae." Krzywicki K.A., Brandriss M.C. Mol. Cell. Biol. 4:2837-2842(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A regulatory region responsible for proline-specific induction of the yeast PUT2 gene is adjacent to its TATA box." Siddiqui A.H., Brandriss M.C. Mol. Cell. Biol. 8:4634-4641(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3.
[5]	"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex." Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M. Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M10029 Genomic DNA. Translation: AAA34924.1. M22785 Genomic DNA. No translation available. U00062 Genomic DNA. Translation: AAB68907.1. BK006934 Genomic DNA. Translation: DAA06728.1.
PIR	RDBYC. S46738.
RefSeq	NP_011902.1. NM_001179167.1.
3D structure databases
ProteinModelPortal	P07275.
SMR	P07275. Positions 28-570.
ModBase	Search...
Protein-protein interaction databases
IntAct	P07275. 2 interactions.
MINT	MINT-640995.
STRING	4932.YHR037W.
Proteomic databases
PaxDb	P07275.
PeptideAtlas	P07275.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR037W; YHR037W; YHR037W.
GeneID	856432.
KEGG	sce:YHR037W.
Organism-specific databases
CYGD	YHR037w.
SGD	S000001079. PUT2.
Phylogenomic databases
eggNOG	COG1012.
GeneTree	ENSGT00560000077335.
HOGENOM	HOG000271511.
KO	K00294.
OMA	NAWQAEI.
OrthoDB	EOG4350FG.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-646.
UniPathway	UPA00261; UER00374.
Gene expression databases
Genevestigator	P07275.
GermOnline	YHR037W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.309.10. 1 hit. 3.40.605.10. 1 hit.
InterPro	IPR005931. 1-pyrroline-5-COlate_DH. IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. [Graphical view]
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR01236. D1pyr5carbox1. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982024.

Entry information

Entry name

PUT2_YEAST

Accession

Primary (citable) accession number: P07275
Secondary accession number(s): D3DKY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 136 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents