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Protein

Profilin

Gene

PFY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.

GO - Molecular functioni

  • actin monomer binding Source: SGD
  • phosphatidylinositol-4,5-bisphosphate binding Source: SGD
  • proline-rich region binding Source: SGD

GO - Biological processi

  • intracellular transport Source: SGD
  • positive regulation of formin-nucleated actin cable assembly Source: SGD
  • sequestering of actin monomers Source: SGD

Keywordsi

Molecular functionActin-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33649-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Profilin
Gene namesi
Name:PFY1
Synonyms:PFY, PRF1
Ordered Locus Names:YOR122C
ORF Names:O3275, YOR3275C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR122C.
SGDiS000005648. PFY1.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: GO_Central
  • cell cortex Source: GO_Central
  • cytosol Source: SGD
  • extrinsic component of plasma membrane Source: SGD

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001996081 – 126ProfilinAdd BLAST126

Proteomic databases

MaxQBiP07274.
PRIDEiP07274.

PTM databases

iPTMnetiP07274.

Interactioni

Subunit structurei

Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio.

Binary interactionsi

WithEntry#Exp.IntActNotes
Bin1Q9VEX93EBI-13892,EBI-129424From Drosophila melanogaster.

GO - Molecular functioni

  • actin monomer binding Source: SGD
  • proline-rich region binding Source: SGD

Protein-protein interaction databases

BioGridi34517. 730 interactors.
DIPiDIP-46N.
IntActiP07274. 4 interactors.
MINTiMINT-575006.
STRINGi4932.YOR122C.

Structurei

Secondary structure

1126
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 12Combined sources10
Beta strandi17 – 23Combined sources7
Beta strandi29 – 34Combined sources6
Helixi40 – 49Combined sources10
Helixi54 – 59Combined sources6
Beta strandi61 – 63Combined sources3
Beta strandi66 – 73Combined sources8
Beta strandi75 – 82Combined sources8
Beta strandi85 – 91Combined sources7
Beta strandi93 – 101Combined sources9
Helixi107 – 123Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K0KX-ray2.35A2-126[»]
1YPRX-ray2.30A/B2-126[»]
ProteinModelPortaliP07274.
SMRiP07274.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07274.

Family & Domainsi

Sequence similaritiesi

Belongs to the profilin family.Curated

Phylogenomic databases

GeneTreeiENSGT00730000112841.
HOGENOMiHOG000171591.
InParanoidiP07274.
KOiK05759.
OMAiILIAHYP.
OrthoDBiEOG092C53JE.

Family and domain databases

CDDicd00148. PROF. 1 hit.
InterProiView protein in InterPro
IPR005455. PFN.
IPR027310. Profilin_CS.
PANTHERiPTHR11604. PTHR11604. 1 hit.
PfamiView protein in Pfam
PF00235. Profilin. 1 hit.
PRINTSiPR00392. PROFILIN.
PR01640. PROFILINPLNT.
SMARTiView protein in SMART
SM00392. PROF. 1 hit.
SUPFAMiSSF55770. SSF55770. 1 hit.
PROSITEiView protein in PROSITE
PS00414. PROFILIN. 1 hit.

Sequencei

Sequence statusi: Complete.

P07274-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWQAYTDNL IGTGKVDKAV IYSRAGDAVW ATSGGLSLQP NEIGEIVQGF
60 70 80 90 100
DNPAGLQSNG LHIQGQKFML LRADDRSIYG RHDAEGVVCV RTKQTVIIAH
110 120
YPPTVQAGEA TKIVEQLADY LIGVQY
Length:126
Mass (Da):13,677
Last modified:November 1, 1990 - v2
Checksum:i2485E413AB30DEAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23369 Genomic DNA. Translation: AAA34861.1.
Y00469 mRNA. Translation: CAA68532.1.
X90518 Genomic DNA. Translation: CAA62128.1.
X94335 Genomic DNA. Translation: CAA64041.1.
Z75030 Genomic DNA. Translation: CAA99321.1.
BK006948 Genomic DNA. Translation: DAA10896.1.
PIRiA31360.
RefSeqiNP_014765.3. NM_001183541.3.

Genome annotation databases

EnsemblFungiiYOR122C; YOR122C; YOR122C.
GeneIDi854289.
KEGGisce:YOR122C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPROF_YEAST
AccessioniPrimary (citable) accession number: P07274
Secondary accession number(s): D6W2I0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1990
Last modified: July 5, 2017
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names