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Reviewed, UniProtKB/Swiss-Prot P07273 (TFS2_YEAST)

Last modified December 15, 2009. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Transcription elongation factor S-II
Alternative name(s):
    DNA strand transfer protein alpha
      Short name=STP-alpha
    DNA strand transferase 1
    Pyrimidine pathway regulatory protein 2
Gene names
Name: DST1
Synonyms: PPR2
Ordered Locus Names: YGL043W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

Can promote the transfer of one strand of a double-stranded DNA molecule to a homologous single strand and thus may be involved in recombination.

Subcellular location

Nucleus.

Miscellaneous

S-II binds to RNA-polymerase II in the absence of transcription.

Present with 6260 molecules/cell in log phase SD medium. Ref.8

Sequence similarities

Belongs to the TFS-II family.

Contains 1 TFIIS central domain.

Contains 1 TFIIS N-terminal domain.

Contains 1 TFIIS-type zinc finger.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ATG26Q063211EBI-19168,EBI-37737
RPB9P279991EBI-19168,EBI-15798

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Transcription elongation factor S-II
PRO_0000121443

Regions

Domain5 – 7975TFIIS N-terminal
Domain148 – 264117TFIIS central
Zinc finger267 – 30741TFIIS-type

Amino acid modifications

Modified residue1151Phosphoserine Ref.9
Modified residue1161Phosphoserine Ref.9
Modified residue1181Phosphoserine Ref.9

Experimental info

Sequence conflict351V → F in AAA88734. Ref.1
Sequence conflict351V → F in AAA34580. Ref.1
Sequence conflict74 – 774DAIN → AQLI in AAA88734. Ref.1
Sequence conflict74 – 774DAIN → AQLI in AAA34580. Ref.1
Sequence conflict821S → C in AAA88734. Ref.1
Sequence conflict821S → C in AAA34580. Ref.1
Sequence conflict851A → P in AAA88734. Ref.1
Sequence conflict851A → P in AAA34580. Ref.1

Secondary structure

.............................. 309
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07273-1 [UniParc].

Last modified October 1, 1996. Version 4.
Checksum: D133938319C015FF

FASTA30934,843
        10         20         30         40         50         60 
MDSKEVLVHV KNLEKNKSND AAVLEILHVL DKEFVPTEKL LRETKVGVEV NKFKKSTNVE 

        70         80         90        100        110        120 
ISKLVKKMIS SWKDAINKNK RSRQAQQHHQ DHAPGNAEDK TTVGESVNGV QQPASSQSDA 

       130        140        150        160        170        180 
MKQDKYVSTK PRNSKNDGVD TAIYHHKLRD QVLKALYDVL AKESEHPPQS ILHTAKAIES 

       190        200        210        220        230        240 
EMNKVNNCDT NEAAYKARYR IIYSNVISKN NPDLKHKIAN GDITPEFLAT CDAKDLAPAP 

       250        260        270        280        290        300 
LKQKIEEIAK QNLYNAQGAT IERSVTDRFT CGKCKEKKVS YYQLQTRSAD EPLTTFCTCE 


ACGNRWKFS 

« Hide

References

« Hide 'large scale' references
[1]"Isolation, DNA sequence, and regulation of a Saccharomyces cerevisiae gene that encodes DNA strand transfer protein alpha."
Clark A.B., Dykstra C.C., Sugino A.
Mol. Cell. Biol. 11:2576-2582(1991) [PubMed: 1850099] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 18-30.
[2]"Purification, gene cloning, and gene disruption of the transcription elongation factor S-II in Saccharomyces cerevisiae."
Nakanishi T., Nakano A., Sekimizu K., Natori S.
J. Biol. Chem. 267:13200-13204(1992) [PubMed: 1618824] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
Feuermann M., de Montigny J., Potier S., Souciet J.-L.
Yeast 13:861-869(1997) [PubMed: 9234674] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed: 9169869] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[5]"Complete sequence of a eukaryotic regulatory gene."
Hubert J.-C., Guyonvarch A., Kammerer B., Exinger F., Liljelund P., Lacroute F.
EMBO J. 2:2071-2073(1983) [PubMed: 6139279] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 182-309.
[6]"Homologue of TFIIS in yeast."
Davies C.J., Trgovchich J., Hutchison C.A. III
Nature 345:298-298(1990) [PubMed: 1971423] [Abstract]
Cited for: SIMILARITY TO S-II.
[7]"TFIIS and strand-transfer proteins."
Kipling D., Kearsey S.E.
Nature 353:509-509(1991) [PubMed: 1922360] [Abstract]
Cited for: IDENTITY OF PPR2 AND DST1.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-115; SER-116 AND SER-118, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

M36724 Genomic DNA. Translation: AAA88734.1.
D12478 Genomic DNA. Translation: BAA02046.1.
M60770 Genomic DNA. Translation: AAA34580.1.
Z72565 Genomic DNA. Translation: CAA96744.1.
X00047 Genomic DNA. Translation: CAA24928.1.
PIRA42921.
RefSeqNP_011472.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1ENWNMR-A131-240[»]
1EO0NMR-A1-77[»]
1PQVX-ray3.80S1-309[»]
1Y1VX-ray3.80S131-309[»]
1Y1YX-ray4.00S131-309[»]
3GTMX-ray3.80S145-309[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-2307N.
IntActP07273. 2 interactions.
STRINGP07273.

Proteomic databases

PeptideAtlasP07273.

Genome annotation databases

EnsemblYGL043W; YGL043W; YGL043W; Saccharomyces cerevisiae. [Genome view]
GeneID852839.
KEGGsce:YGL043W.
NMPDRfig|4932.3.peg.2577.

Organism-specific databases

CYGDYGL043w.
SGDS000003011. DST1.

Phylogenomic databases

HOGENOMHBG716813.
OrthoDBEOG9D80PG.

Gene expression databases

ArrayExpressP07273.
GenevestigatorP07273.
GermOnlineYGL043W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR016492. TF_IIS.
IPR003617. TFIIS/CRSP70_N_sub.
IPR010990. TFIIS/elonginA/CRSP70_N.
IPR003618. TFIIS_centre.
IPR017923. TFIIS_N.
IPR014754. TFIIS_N_fun-typ.
IPR017890. TFS2M.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
Gene3DG3DSA:1.10.472.30. TFIIS_centre. 1 hit.
G3DSA:1.20.930.10. TFIIS_N_fun-typ. 1 hit.
PfamPF08711. TFIIS. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFPIRSF006704. TF_IIS. 1 hit.
SMARTSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
PROSITEPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio972417.

Entry information

Entry nameTFS2_YEAST
AccessionPrimary (citable) accession number: P07273
Secondary accession number(s): P24535
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: October 1, 1996
Last modified: December 15, 2009
This is version 110 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents