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P07273

- TFS2_YEAST

UniProt

P07273 - TFS2_YEAST

Protein

Transcription elongation factor S-II

Gene

DST1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 156 (01 Oct 2014)
      Sequence version 4 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
    Can promote the transfer of one strand of a double-stranded DNA molecule to a homologous single strand and thus may be involved in recombination.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri267 – 30741TFIIS-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. core RNA polymerase II recruiting transcription factor activity Source: SGD
    2. protein binding Source: IntAct
    3. RNA polymerase II core binding Source: SGD
    4. RNA polymerase II regulatory region DNA binding Source: SGD
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter Source: SGD
    2. mRNA cleavage Source: SGD
    3. positive regulation of RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
    4. positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
    5. regulation of mRNA 3'-end processing Source: SGD
    6. RNA polymerase II transcriptional preinitiation complex assembly Source: GOC
    7. transcription antitermination Source: SGD
    8. transcription elongation from RNA polymerase II promoter Source: SGD
    9. transcription elongation from RNA polymerase I promoter Source: SGD
    10. transcription from RNA polymerase III promoter Source: SGD
    11. transcription initiation from RNA polymerase II promoter Source: SGD
    12. tRNA transcription from RNA polymerase III promoter Source: SGD

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30554-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription elongation factor S-II
    Alternative name(s):
    DNA strand transfer protein alpha
    Short name:
    STP-alpha
    DNA strand transferase 1
    Pyrimidine pathway regulatory protein 2
    Gene namesi
    Name:DST1
    Synonyms:PPR2
    Ordered Locus Names:YGL043W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL043w.
    SGDiS000003011. DST1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309Transcription elongation factor S-IIPRO_0000121443Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei116 – 1161Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP07273.
    PaxDbiP07273.
    PeptideAtlasiP07273.

    Expressioni

    Gene expression databases

    GenevestigatoriP07273.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SPT8P389152EBI-19168,EBI-17964
    SSN2P389312EBI-19168,EBI-18059

    Protein-protein interaction databases

    BioGridi33205. 273 interactions.
    DIPiDIP-2307N.
    IntActiP07273. 6 interactions.
    MINTiMINT-1167083.
    STRINGi4932.YGL043W.

    Structurei

    Secondary structure

    1
    309
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 1614
    Beta strandi18 – 203
    Helixi21 – 3010
    Turni31 – 333
    Helixi40 – 5213
    Beta strandi55 – 573
    Helixi61 – 7616
    Turni134 – 1374
    Helixi147 – 15913
    Beta strandi162 – 1654
    Helixi170 – 18516
    Beta strandi189 – 1924
    Helixi194 – 20714
    Beta strandi208 – 2103
    Helixi213 – 2208
    Helixi226 – 2294
    Turni232 – 2354
    Helixi240 – 25516
    Beta strandi266 – 2694
    Beta strandi272 – 2743
    Beta strandi297 – 2993
    Turni300 – 3023

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ENWNMR-A131-240[»]
    1EO0NMR-A1-77[»]
    1PQVX-ray3.80S1-309[»]
    1Y1VX-ray3.80S131-309[»]
    1Y1YX-ray4.00S131-309[»]
    3GTMX-ray3.80S147-309[»]
    3PO3X-ray3.30S132-309[»]
    ProteinModelPortaliP07273.
    SMRiP07273. Positions 1-77, 147-309.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07273.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 7975TFIIS N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini148 – 264117TFIIS centralPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TFS-II family.Curated
    Contains 1 TFIIS central domain.PROSITE-ProRule annotation
    Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation
    Contains 1 TFIIS-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri267 – 30741TFIIS-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG1594.
    GeneTreeiENSGT00390000017794.
    HOGENOMiHOG000195015.
    KOiK03145.
    OMAiVNIYSAV.
    OrthoDBiEOG7DNP5M.

    Family and domain databases

    Gene3Di1.10.472.30. 1 hit.
    1.20.930.10. 1 hit.
    InterProiIPR016492. TF_IIS-rel.
    IPR003617. TFIIS/CRSP70_N_sub.
    IPR003618. TFIIS_cen_dom.
    IPR017923. TFIIS_N.
    IPR017890. TFS2M.
    IPR006289. TFSII.
    IPR001222. Znf_TFIIS.
    [Graphical view]
    PfamiPF08711. Med26. 1 hit.
    PF01096. TFIIS_C. 1 hit.
    PF07500. TFIIS_M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006704. TF_IIS. 1 hit.
    SMARTiSM00510. TFS2M. 1 hit.
    SM00509. TFS2N. 1 hit.
    SM00440. ZnF_C2C2. 1 hit.
    [Graphical view]
    SUPFAMiSSF46942. SSF46942. 1 hit.
    SSF47676. SSF47676. 1 hit.
    TIGRFAMsiTIGR01385. TFSII. 1 hit.
    PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
    PS51319. TFIIS_N. 1 hit.
    PS00466. ZF_TFIIS_1. 1 hit.
    PS51133. ZF_TFIIS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07273-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSKEVLVHV KNLEKNKSND AAVLEILHVL DKEFVPTEKL LRETKVGVEV    50
    NKFKKSTNVE ISKLVKKMIS SWKDAINKNK RSRQAQQHHQ DHAPGNAEDK 100
    TTVGESVNGV QQPASSQSDA MKQDKYVSTK PRNSKNDGVD TAIYHHKLRD 150
    QVLKALYDVL AKESEHPPQS ILHTAKAIES EMNKVNNCDT NEAAYKARYR 200
    IIYSNVISKN NPDLKHKIAN GDITPEFLAT CDAKDLAPAP LKQKIEEIAK 250
    QNLYNAQGAT IERSVTDRFT CGKCKEKKVS YYQLQTRSAD EPLTTFCTCE 300
    ACGNRWKFS 309
    Length:309
    Mass (Da):34,843
    Last modified:October 1, 1996 - v4
    Checksum:iD133938319C015FF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti35 – 351V → F in AAA88734. (PubMed:1850099)Curated
    Sequence conflicti35 – 351V → F in AAA34580. (PubMed:1850099)Curated
    Sequence conflicti74 – 774DAIN → AQLI in AAA88734. (PubMed:1850099)Curated
    Sequence conflicti74 – 774DAIN → AQLI in AAA34580. (PubMed:1850099)Curated
    Sequence conflicti82 – 821S → C in AAA88734. (PubMed:1850099)Curated
    Sequence conflicti82 – 821S → C in AAA34580. (PubMed:1850099)Curated
    Sequence conflicti85 – 851A → P in AAA88734. (PubMed:1850099)Curated
    Sequence conflicti85 – 851A → P in AAA34580. (PubMed:1850099)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36724 Genomic DNA. Translation: AAA88734.1.
    D12478 Genomic DNA. Translation: BAA02046.1.
    M60770 Genomic DNA. Translation: AAA34580.1.
    Z72565 Genomic DNA. Translation: CAA96744.1.
    X00047 Genomic DNA. Translation: CAA24928.1.
    BK006941 Genomic DNA. Translation: DAA08057.1.
    PIRiA42921.
    RefSeqiNP_011472.1. NM_001180908.1.

    Genome annotation databases

    EnsemblFungiiYGL043W; YGL043W; YGL043W.
    GeneIDi852839.
    KEGGisce:YGL043W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36724 Genomic DNA. Translation: AAA88734.1 .
    D12478 Genomic DNA. Translation: BAA02046.1 .
    M60770 Genomic DNA. Translation: AAA34580.1 .
    Z72565 Genomic DNA. Translation: CAA96744.1 .
    X00047 Genomic DNA. Translation: CAA24928.1 .
    BK006941 Genomic DNA. Translation: DAA08057.1 .
    PIRi A42921.
    RefSeqi NP_011472.1. NM_001180908.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ENW NMR - A 131-240 [» ]
    1EO0 NMR - A 1-77 [» ]
    1PQV X-ray 3.80 S 1-309 [» ]
    1Y1V X-ray 3.80 S 131-309 [» ]
    1Y1Y X-ray 4.00 S 131-309 [» ]
    3GTM X-ray 3.80 S 147-309 [» ]
    3PO3 X-ray 3.30 S 132-309 [» ]
    ProteinModelPortali P07273.
    SMRi P07273. Positions 1-77, 147-309.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33205. 273 interactions.
    DIPi DIP-2307N.
    IntActi P07273. 6 interactions.
    MINTi MINT-1167083.
    STRINGi 4932.YGL043W.

    Proteomic databases

    MaxQBi P07273.
    PaxDbi P07273.
    PeptideAtlasi P07273.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL043W ; YGL043W ; YGL043W .
    GeneIDi 852839.
    KEGGi sce:YGL043W.

    Organism-specific databases

    CYGDi YGL043w.
    SGDi S000003011. DST1.

    Phylogenomic databases

    eggNOGi COG1594.
    GeneTreei ENSGT00390000017794.
    HOGENOMi HOG000195015.
    KOi K03145.
    OMAi VNIYSAV.
    OrthoDBi EOG7DNP5M.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30554-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P07273.
    NextBioi 972417.
    PROi P07273.

    Gene expression databases

    Genevestigatori P07273.

    Family and domain databases

    Gene3Di 1.10.472.30. 1 hit.
    1.20.930.10. 1 hit.
    InterProi IPR016492. TF_IIS-rel.
    IPR003617. TFIIS/CRSP70_N_sub.
    IPR003618. TFIIS_cen_dom.
    IPR017923. TFIIS_N.
    IPR017890. TFS2M.
    IPR006289. TFSII.
    IPR001222. Znf_TFIIS.
    [Graphical view ]
    Pfami PF08711. Med26. 1 hit.
    PF01096. TFIIS_C. 1 hit.
    PF07500. TFIIS_M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006704. TF_IIS. 1 hit.
    SMARTi SM00510. TFS2M. 1 hit.
    SM00509. TFS2N. 1 hit.
    SM00440. ZnF_C2C2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46942. SSF46942. 1 hit.
    SSF47676. SSF47676. 1 hit.
    TIGRFAMsi TIGR01385. TFSII. 1 hit.
    PROSITEi PS51321. TFIIS_CENTRAL. 1 hit.
    PS51319. TFIIS_N. 1 hit.
    PS00466. ZF_TFIIS_1. 1 hit.
    PS51133. ZF_TFIIS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, DNA sequence, and regulation of a Saccharomyces cerevisiae gene that encodes DNA strand transfer protein alpha."
      Clark A.B., Dykstra C.C., Sugino A.
      Mol. Cell. Biol. 11:2576-2582(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 18-30.
    2. "Purification, gene cloning, and gene disruption of the transcription elongation factor S-II in Saccharomyces cerevisiae."
      Nakanishi T., Nakano A., Sekimizu K., Natori S.
      J. Biol. Chem. 267:13200-13204(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
      Feuermann M., de Montigny J., Potier S., Souciet J.-L.
      Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Complete sequence of a eukaryotic regulatory gene."
      Hubert J.-C., Guyonvarch A., Kammerer B., Exinger F., Liljelund P., Lacroute F.
      EMBO J. 2:2071-2073(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 182-309.
    7. Cited for: SIMILARITY TO S-II.
    8. "TFIIS and strand-transfer proteins."
      Kipling D., Kearsey S.E.
      Nature 353:509-509(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTITY OF PPR2 AND DST1.
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTFS2_YEAST
    AccessioniPrimary (citable) accession number: P07273
    Secondary accession number(s): D6VU96, P24535
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 156 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    S-II binds to RNA-polymerase II in the absence of transcription.
    Present with 6260 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3