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Protein

Transcription elongation factor S-II

Gene

DST1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Can promote the transfer of one strand of a double-stranded DNA molecule to a homologous single strand and thus may be involved in recombination.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri267 – 307TFIIS-typePROSITE-ProRule annotationAdd BLAST41

GO - Molecular functioni

  • RNA polymerase II core binding Source: SGD
  • RNA polymerase II regulatory region DNA binding Source: SGD
  • transcription factor activity, core RNA polymerase II recruiting Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter Source: SGD
  • positive regulation of RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • regulation of mRNA 3'-end processing Source: SGD
  • transcription antitermination Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: SGD
  • transcription elongation from RNA polymerase I promoter Source: SGD
  • transcription from RNA polymerase III promoter Source: SGD
  • transcription initiation from RNA polymerase II promoter Source: SGD
  • tRNA transcription from RNA polymerase III promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30554-MONOMER.
ReactomeiR-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor S-II
Alternative name(s):
DNA strand transfer protein alpha
Short name:
STP-alpha
DNA strand transferase 1
Pyrimidine pathway regulatory protein 2
Gene namesi
Name:DST1
Synonyms:PPR2
Ordered Locus Names:YGL043W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL043W.
SGDiS000003011. DST1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001214431 – 309Transcription elongation factor S-IIAdd BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei116PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07273.
PRIDEiP07273.

PTM databases

iPTMnetiP07273.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SPT8P389152EBI-19168,EBI-17964
SSN2P389312EBI-19168,EBI-18059

GO - Molecular functioni

  • RNA polymerase II core binding Source: SGD

Protein-protein interaction databases

BioGridi33205. 274 interactors.
DIPiDIP-2307N.
IntActiP07273. 6 interactors.
MINTiMINT-1167083.

Structurei

Secondary structure

1309
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 16Combined sources14
Beta strandi18 – 20Combined sources3
Helixi21 – 30Combined sources10
Turni31 – 33Combined sources3
Helixi40 – 52Combined sources13
Beta strandi55 – 57Combined sources3
Helixi61 – 76Combined sources16
Turni134 – 137Combined sources4
Helixi147 – 159Combined sources13
Beta strandi162 – 165Combined sources4
Helixi170 – 185Combined sources16
Beta strandi189 – 192Combined sources4
Helixi194 – 207Combined sources14
Beta strandi208 – 210Combined sources3
Helixi213 – 220Combined sources8
Helixi226 – 229Combined sources4
Turni232 – 235Combined sources4
Helixi240 – 255Combined sources16
Beta strandi266 – 269Combined sources4
Beta strandi272 – 274Combined sources3
Beta strandi297 – 299Combined sources3
Turni300 – 302Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ENWNMR-A131-240[»]
1EO0NMR-A1-77[»]
1PQVX-ray3.80S1-309[»]
1Y1VX-ray3.80S131-309[»]
1Y1YX-ray4.00S131-309[»]
3GTMX-ray3.80S147-309[»]
3PO3X-ray3.30S132-309[»]
5FMFelectron microscopy6.002136-309[»]
ProteinModelPortaliP07273.
SMRiP07273.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07273.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 79TFIIS N-terminalPROSITE-ProRule annotationAdd BLAST75
Domaini148 – 264TFIIS centralPROSITE-ProRule annotationAdd BLAST117

Sequence similaritiesi

Belongs to the TFS-II family.Curated
Contains 1 TFIIS central domain.PROSITE-ProRule annotation
Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation
Contains 1 TFIIS-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri267 – 307TFIIS-typePROSITE-ProRule annotationAdd BLAST41

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
InParanoidiP07273.
KOiK03145.
OMAiKLRTFVM.
OrthoDBiEOG092C495C.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07273-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSKEVLVHV KNLEKNKSND AAVLEILHVL DKEFVPTEKL LRETKVGVEV
60 70 80 90 100
NKFKKSTNVE ISKLVKKMIS SWKDAINKNK RSRQAQQHHQ DHAPGNAEDK
110 120 130 140 150
TTVGESVNGV QQPASSQSDA MKQDKYVSTK PRNSKNDGVD TAIYHHKLRD
160 170 180 190 200
QVLKALYDVL AKESEHPPQS ILHTAKAIES EMNKVNNCDT NEAAYKARYR
210 220 230 240 250
IIYSNVISKN NPDLKHKIAN GDITPEFLAT CDAKDLAPAP LKQKIEEIAK
260 270 280 290 300
QNLYNAQGAT IERSVTDRFT CGKCKEKKVS YYQLQTRSAD EPLTTFCTCE

ACGNRWKFS
Length:309
Mass (Da):34,843
Last modified:October 1, 1996 - v4
Checksum:iD133938319C015FF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35V → F in AAA88734 (PubMed:1850099).Curated1
Sequence conflicti35V → F in AAA34580 (PubMed:1850099).Curated1
Sequence conflicti74 – 77DAIN → AQLI in AAA88734 (PubMed:1850099).Curated4
Sequence conflicti74 – 77DAIN → AQLI in AAA34580 (PubMed:1850099).Curated4
Sequence conflicti82S → C in AAA88734 (PubMed:1850099).Curated1
Sequence conflicti82S → C in AAA34580 (PubMed:1850099).Curated1
Sequence conflicti85A → P in AAA88734 (PubMed:1850099).Curated1
Sequence conflicti85A → P in AAA34580 (PubMed:1850099).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36724 Genomic DNA. Translation: AAA88734.1.
D12478 Genomic DNA. Translation: BAA02046.1.
M60770 Genomic DNA. Translation: AAA34580.1.
Z72565 Genomic DNA. Translation: CAA96744.1.
X00047 Genomic DNA. Translation: CAA24928.1.
BK006941 Genomic DNA. Translation: DAA08057.1.
PIRiA42921.
RefSeqiNP_011472.1. NM_001180908.1.

Genome annotation databases

EnsemblFungiiYGL043W; YGL043W; YGL043W.
GeneIDi852839.
KEGGisce:YGL043W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36724 Genomic DNA. Translation: AAA88734.1.
D12478 Genomic DNA. Translation: BAA02046.1.
M60770 Genomic DNA. Translation: AAA34580.1.
Z72565 Genomic DNA. Translation: CAA96744.1.
X00047 Genomic DNA. Translation: CAA24928.1.
BK006941 Genomic DNA. Translation: DAA08057.1.
PIRiA42921.
RefSeqiNP_011472.1. NM_001180908.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ENWNMR-A131-240[»]
1EO0NMR-A1-77[»]
1PQVX-ray3.80S1-309[»]
1Y1VX-ray3.80S131-309[»]
1Y1YX-ray4.00S131-309[»]
3GTMX-ray3.80S147-309[»]
3PO3X-ray3.30S132-309[»]
5FMFelectron microscopy6.002136-309[»]
ProteinModelPortaliP07273.
SMRiP07273.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33205. 274 interactors.
DIPiDIP-2307N.
IntActiP07273. 6 interactors.
MINTiMINT-1167083.

PTM databases

iPTMnetiP07273.

Proteomic databases

MaxQBiP07273.
PRIDEiP07273.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL043W; YGL043W; YGL043W.
GeneIDi852839.
KEGGisce:YGL043W.

Organism-specific databases

EuPathDBiFungiDB:YGL043W.
SGDiS000003011. DST1.

Phylogenomic databases

GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
InParanoidiP07273.
KOiK03145.
OMAiKLRTFVM.
OrthoDBiEOG092C495C.

Enzyme and pathway databases

BioCyciYEAST:G3O-30554-MONOMER.
ReactomeiR-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.

Miscellaneous databases

EvolutionaryTraceiP07273.
PROiP07273.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTFS2_YEAST
AccessioniPrimary (citable) accession number: P07273
Secondary accession number(s): D6VU96, P24535
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 177 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

S-II binds to RNA-polymerase II in the absence of transcription.
Present with 6260 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.