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Protein

Transcription elongation factor S-II

Gene

DST1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Can promote the transfer of one strand of a double-stranded DNA molecule to a homologous single strand and thus may be involved in recombination.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri267 – 30741TFIIS-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. core RNA polymerase II recruiting transcription factor activity Source: SGD
  2. RNA polymerase II core binding Source: SGD
  3. RNA polymerase II regulatory region DNA binding Source: SGD
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter Source: SGD
  2. mRNA cleavage Source: SGD
  3. positive regulation of RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  4. positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  5. regulation of mRNA 3'-end processing Source: SGD
  6. RNA polymerase II transcriptional preinitiation complex assembly Source: GOC
  7. transcription antitermination Source: SGD
  8. transcription elongation from RNA polymerase II promoter Source: SGD
  9. transcription elongation from RNA polymerase I promoter Source: SGD
  10. transcription from RNA polymerase III promoter Source: SGD
  11. transcription initiation from RNA polymerase II promoter Source: SGD
  12. tRNA transcription from RNA polymerase III promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30554-MONOMER.
ReactomeiREACT_235070. Dual incision reaction in TC-NER.
REACT_247354. Formation of transcription-coupled NER (TC-NER) repair complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor S-II
Alternative name(s):
DNA strand transfer protein alpha
Short name:
STP-alpha
DNA strand transferase 1
Pyrimidine pathway regulatory protein 2
Gene namesi
Name:DST1
Synonyms:PPR2
Ordered Locus Names:YGL043W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL043w.
SGDiS000003011. DST1.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Transcription elongation factor S-IIPRO_0000121443Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei116 – 1161Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07273.
PaxDbiP07273.
PeptideAtlasiP07273.

Expressioni

Gene expression databases

GenevestigatoriP07273.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SPT8P389152EBI-19168,EBI-17964
SSN2P389312EBI-19168,EBI-18059

Protein-protein interaction databases

BioGridi33205. 274 interactions.
DIPiDIP-2307N.
IntActiP07273. 6 interactions.
MINTiMINT-1167083.
STRINGi4932.YGL043W.

Structurei

Secondary structure

1
309
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1614Combined sources
Beta strandi18 – 203Combined sources
Helixi21 – 3010Combined sources
Turni31 – 333Combined sources
Helixi40 – 5213Combined sources
Beta strandi55 – 573Combined sources
Helixi61 – 7616Combined sources
Turni134 – 1374Combined sources
Helixi147 – 15913Combined sources
Beta strandi162 – 1654Combined sources
Helixi170 – 18516Combined sources
Beta strandi189 – 1924Combined sources
Helixi194 – 20714Combined sources
Beta strandi208 – 2103Combined sources
Helixi213 – 2208Combined sources
Helixi226 – 2294Combined sources
Turni232 – 2354Combined sources
Helixi240 – 25516Combined sources
Beta strandi266 – 2694Combined sources
Beta strandi272 – 2743Combined sources
Beta strandi297 – 2993Combined sources
Turni300 – 3023Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ENWNMR-A131-240[»]
1EO0NMR-A1-77[»]
1PQVX-ray3.80S1-309[»]
1Y1VX-ray3.80S131-309[»]
1Y1YX-ray4.00S131-309[»]
3GTMX-ray3.80S147-309[»]
3PO3X-ray3.30S132-309[»]
ProteinModelPortaliP07273.
SMRiP07273. Positions 1-77, 147-309.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07273.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 7975TFIIS N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini148 – 264117TFIIS centralPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TFS-II family.Curated
Contains 1 TFIIS central domain.PROSITE-ProRule annotation
Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation
Contains 1 TFIIS-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri267 – 30741TFIIS-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG1594.
GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
InParanoidiP07273.
KOiK03145.
OMAiESEHPPQ.
OrthoDBiEOG7DNP5M.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR017890. TFS2M.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07273-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDSKEVLVHV KNLEKNKSND AAVLEILHVL DKEFVPTEKL LRETKVGVEV
60 70 80 90 100
NKFKKSTNVE ISKLVKKMIS SWKDAINKNK RSRQAQQHHQ DHAPGNAEDK
110 120 130 140 150
TTVGESVNGV QQPASSQSDA MKQDKYVSTK PRNSKNDGVD TAIYHHKLRD
160 170 180 190 200
QVLKALYDVL AKESEHPPQS ILHTAKAIES EMNKVNNCDT NEAAYKARYR
210 220 230 240 250
IIYSNVISKN NPDLKHKIAN GDITPEFLAT CDAKDLAPAP LKQKIEEIAK
260 270 280 290 300
QNLYNAQGAT IERSVTDRFT CGKCKEKKVS YYQLQTRSAD EPLTTFCTCE

ACGNRWKFS
Length:309
Mass (Da):34,843
Last modified:October 1, 1996 - v4
Checksum:iD133938319C015FF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351V → F in AAA88734. (PubMed:1850099)Curated
Sequence conflicti35 – 351V → F in AAA34580. (PubMed:1850099)Curated
Sequence conflicti74 – 774DAIN → AQLI in AAA88734. (PubMed:1850099)Curated
Sequence conflicti74 – 774DAIN → AQLI in AAA34580. (PubMed:1850099)Curated
Sequence conflicti82 – 821S → C in AAA88734. (PubMed:1850099)Curated
Sequence conflicti82 – 821S → C in AAA34580. (PubMed:1850099)Curated
Sequence conflicti85 – 851A → P in AAA88734. (PubMed:1850099)Curated
Sequence conflicti85 – 851A → P in AAA34580. (PubMed:1850099)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36724 Genomic DNA. Translation: AAA88734.1.
D12478 Genomic DNA. Translation: BAA02046.1.
M60770 Genomic DNA. Translation: AAA34580.1.
Z72565 Genomic DNA. Translation: CAA96744.1.
X00047 Genomic DNA. Translation: CAA24928.1.
BK006941 Genomic DNA. Translation: DAA08057.1.
PIRiA42921.
RefSeqiNP_011472.1. NM_001180908.1.

Genome annotation databases

EnsemblFungiiYGL043W; YGL043W; YGL043W.
GeneIDi852839.
KEGGisce:YGL043W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36724 Genomic DNA. Translation: AAA88734.1.
D12478 Genomic DNA. Translation: BAA02046.1.
M60770 Genomic DNA. Translation: AAA34580.1.
Z72565 Genomic DNA. Translation: CAA96744.1.
X00047 Genomic DNA. Translation: CAA24928.1.
BK006941 Genomic DNA. Translation: DAA08057.1.
PIRiA42921.
RefSeqiNP_011472.1. NM_001180908.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ENWNMR-A131-240[»]
1EO0NMR-A1-77[»]
1PQVX-ray3.80S1-309[»]
1Y1VX-ray3.80S131-309[»]
1Y1YX-ray4.00S131-309[»]
3GTMX-ray3.80S147-309[»]
3PO3X-ray3.30S132-309[»]
ProteinModelPortaliP07273.
SMRiP07273. Positions 1-77, 147-309.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33205. 274 interactions.
DIPiDIP-2307N.
IntActiP07273. 6 interactions.
MINTiMINT-1167083.
STRINGi4932.YGL043W.

Proteomic databases

MaxQBiP07273.
PaxDbiP07273.
PeptideAtlasiP07273.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL043W; YGL043W; YGL043W.
GeneIDi852839.
KEGGisce:YGL043W.

Organism-specific databases

CYGDiYGL043w.
SGDiS000003011. DST1.

Phylogenomic databases

eggNOGiCOG1594.
GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
InParanoidiP07273.
KOiK03145.
OMAiESEHPPQ.
OrthoDBiEOG7DNP5M.

Enzyme and pathway databases

BioCyciYEAST:G3O-30554-MONOMER.
ReactomeiREACT_235070. Dual incision reaction in TC-NER.
REACT_247354. Formation of transcription-coupled NER (TC-NER) repair complex.

Miscellaneous databases

EvolutionaryTraceiP07273.
NextBioi972417.
PROiP07273.

Gene expression databases

GenevestigatoriP07273.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR017890. TFS2M.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, DNA sequence, and regulation of a Saccharomyces cerevisiae gene that encodes DNA strand transfer protein alpha."
    Clark A.B., Dykstra C.C., Sugino A.
    Mol. Cell. Biol. 11:2576-2582(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 18-30.
  2. "Purification, gene cloning, and gene disruption of the transcription elongation factor S-II in Saccharomyces cerevisiae."
    Nakanishi T., Nakano A., Sekimizu K., Natori S.
    J. Biol. Chem. 267:13200-13204(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
    Feuermann M., de Montigny J., Potier S., Souciet J.-L.
    Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Complete sequence of a eukaryotic regulatory gene."
    Hubert J.-C., Guyonvarch A., Kammerer B., Exinger F., Liljelund P., Lacroute F.
    EMBO J. 2:2071-2073(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 182-309.
  7. Cited for: SIMILARITY TO S-II.
  8. "TFIIS and strand-transfer proteins."
    Kipling D., Kearsey S.E.
    Nature 353:509-509(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTITY OF PPR2 AND DST1.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTFS2_YEAST
AccessioniPrimary (citable) accession number: P07273
Secondary accession number(s): D6VU96, P24535
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 159 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

S-II binds to RNA-polymerase II in the absence of transcription.
Present with 6260 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.