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P07273

- TFS2_YEAST

UniProt

P07273 - TFS2_YEAST

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Protein

Transcription elongation factor S-II

Gene

DST1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Can promote the transfer of one strand of a double-stranded DNA molecule to a homologous single strand and thus may be involved in recombination.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri267 – 30741TFIIS-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. core RNA polymerase II recruiting transcription factor activity Source: SGD
  2. RNA polymerase II core binding Source: SGD
  3. RNA polymerase II regulatory region DNA binding Source: SGD
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter Source: SGD
  2. mRNA cleavage Source: SGD
  3. positive regulation of RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  4. positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  5. regulation of mRNA 3'-end processing Source: SGD
  6. RNA polymerase II transcriptional preinitiation complex assembly Source: GOC
  7. transcription antitermination Source: SGD
  8. transcription elongation from RNA polymerase II promoter Source: SGD
  9. transcription elongation from RNA polymerase I promoter Source: SGD
  10. transcription from RNA polymerase III promoter Source: SGD
  11. transcription initiation from RNA polymerase II promoter Source: SGD
  12. tRNA transcription from RNA polymerase III promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30554-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor S-II
Alternative name(s):
DNA strand transfer protein alpha
Short name:
STP-alpha
DNA strand transferase 1
Pyrimidine pathway regulatory protein 2
Gene namesi
Name:DST1
Synonyms:PPR2
Ordered Locus Names:YGL043W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL043w.
SGDiS000003011. DST1.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Transcription elongation factor S-IIPRO_0000121443Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei116 – 1161Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07273.
PaxDbiP07273.
PeptideAtlasiP07273.

Expressioni

Gene expression databases

GenevestigatoriP07273.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SPT8P389152EBI-19168,EBI-17964
SSN2P389312EBI-19168,EBI-18059

Protein-protein interaction databases

BioGridi33205. 274 interactions.
DIPiDIP-2307N.
IntActiP07273. 6 interactions.
MINTiMINT-1167083.
STRINGi4932.YGL043W.

Structurei

Secondary structure

1
309
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1614
Beta strandi18 – 203
Helixi21 – 3010
Turni31 – 333
Helixi40 – 5213
Beta strandi55 – 573
Helixi61 – 7616
Turni134 – 1374
Helixi147 – 15913
Beta strandi162 – 1654
Helixi170 – 18516
Beta strandi189 – 1924
Helixi194 – 20714
Beta strandi208 – 2103
Helixi213 – 2208
Helixi226 – 2294
Turni232 – 2354
Helixi240 – 25516
Beta strandi266 – 2694
Beta strandi272 – 2743
Beta strandi297 – 2993
Turni300 – 3023

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ENWNMR-A131-240[»]
1EO0NMR-A1-77[»]
1PQVX-ray3.80S1-309[»]
1Y1VX-ray3.80S131-309[»]
1Y1YX-ray4.00S131-309[»]
3GTMX-ray3.80S147-309[»]
3PO3X-ray3.30S132-309[»]
ProteinModelPortaliP07273.
SMRiP07273. Positions 1-77, 147-309.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07273.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 7975TFIIS N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini148 – 264117TFIIS centralPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TFS-II family.Curated
Contains 1 TFIIS central domain.PROSITE-ProRule annotation
Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation
Contains 1 TFIIS-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri267 – 30741TFIIS-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG1594.
GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
InParanoidiP07273.
KOiK03145.
OMAiVNIYSAV.
OrthoDBiEOG7DNP5M.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR017890. TFS2M.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07273-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDSKEVLVHV KNLEKNKSND AAVLEILHVL DKEFVPTEKL LRETKVGVEV
60 70 80 90 100
NKFKKSTNVE ISKLVKKMIS SWKDAINKNK RSRQAQQHHQ DHAPGNAEDK
110 120 130 140 150
TTVGESVNGV QQPASSQSDA MKQDKYVSTK PRNSKNDGVD TAIYHHKLRD
160 170 180 190 200
QVLKALYDVL AKESEHPPQS ILHTAKAIES EMNKVNNCDT NEAAYKARYR
210 220 230 240 250
IIYSNVISKN NPDLKHKIAN GDITPEFLAT CDAKDLAPAP LKQKIEEIAK
260 270 280 290 300
QNLYNAQGAT IERSVTDRFT CGKCKEKKVS YYQLQTRSAD EPLTTFCTCE

ACGNRWKFS
Length:309
Mass (Da):34,843
Last modified:October 1, 1996 - v4
Checksum:iD133938319C015FF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351V → F in AAA88734. (PubMed:1850099)Curated
Sequence conflicti35 – 351V → F in AAA34580. (PubMed:1850099)Curated
Sequence conflicti74 – 774DAIN → AQLI in AAA88734. (PubMed:1850099)Curated
Sequence conflicti74 – 774DAIN → AQLI in AAA34580. (PubMed:1850099)Curated
Sequence conflicti82 – 821S → C in AAA88734. (PubMed:1850099)Curated
Sequence conflicti82 – 821S → C in AAA34580. (PubMed:1850099)Curated
Sequence conflicti85 – 851A → P in AAA88734. (PubMed:1850099)Curated
Sequence conflicti85 – 851A → P in AAA34580. (PubMed:1850099)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36724 Genomic DNA. Translation: AAA88734.1.
D12478 Genomic DNA. Translation: BAA02046.1.
M60770 Genomic DNA. Translation: AAA34580.1.
Z72565 Genomic DNA. Translation: CAA96744.1.
X00047 Genomic DNA. Translation: CAA24928.1.
BK006941 Genomic DNA. Translation: DAA08057.1.
PIRiA42921.
RefSeqiNP_011472.1. NM_001180908.1.

Genome annotation databases

EnsemblFungiiYGL043W; YGL043W; YGL043W.
GeneIDi852839.
KEGGisce:YGL043W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36724 Genomic DNA. Translation: AAA88734.1 .
D12478 Genomic DNA. Translation: BAA02046.1 .
M60770 Genomic DNA. Translation: AAA34580.1 .
Z72565 Genomic DNA. Translation: CAA96744.1 .
X00047 Genomic DNA. Translation: CAA24928.1 .
BK006941 Genomic DNA. Translation: DAA08057.1 .
PIRi A42921.
RefSeqi NP_011472.1. NM_001180908.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ENW NMR - A 131-240 [» ]
1EO0 NMR - A 1-77 [» ]
1PQV X-ray 3.80 S 1-309 [» ]
1Y1V X-ray 3.80 S 131-309 [» ]
1Y1Y X-ray 4.00 S 131-309 [» ]
3GTM X-ray 3.80 S 147-309 [» ]
3PO3 X-ray 3.30 S 132-309 [» ]
ProteinModelPortali P07273.
SMRi P07273. Positions 1-77, 147-309.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33205. 274 interactions.
DIPi DIP-2307N.
IntActi P07273. 6 interactions.
MINTi MINT-1167083.
STRINGi 4932.YGL043W.

Proteomic databases

MaxQBi P07273.
PaxDbi P07273.
PeptideAtlasi P07273.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL043W ; YGL043W ; YGL043W .
GeneIDi 852839.
KEGGi sce:YGL043W.

Organism-specific databases

CYGDi YGL043w.
SGDi S000003011. DST1.

Phylogenomic databases

eggNOGi COG1594.
GeneTreei ENSGT00390000017794.
HOGENOMi HOG000195015.
InParanoidi P07273.
KOi K03145.
OMAi VNIYSAV.
OrthoDBi EOG7DNP5M.

Enzyme and pathway databases

BioCyci YEAST:G3O-30554-MONOMER.

Miscellaneous databases

EvolutionaryTracei P07273.
NextBioi 972417.
PROi P07273.

Gene expression databases

Genevestigatori P07273.

Family and domain databases

Gene3Di 1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProi IPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR017890. TFS2M.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view ]
Pfami PF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view ]
PIRSFi PIRSF006704. TF_IIS. 1 hit.
SMARTi SM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view ]
SUPFAMi SSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsi TIGR01385. TFSII. 1 hit.
PROSITEi PS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, DNA sequence, and regulation of a Saccharomyces cerevisiae gene that encodes DNA strand transfer protein alpha."
    Clark A.B., Dykstra C.C., Sugino A.
    Mol. Cell. Biol. 11:2576-2582(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 18-30.
  2. "Purification, gene cloning, and gene disruption of the transcription elongation factor S-II in Saccharomyces cerevisiae."
    Nakanishi T., Nakano A., Sekimizu K., Natori S.
    J. Biol. Chem. 267:13200-13204(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
    Feuermann M., de Montigny J., Potier S., Souciet J.-L.
    Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Complete sequence of a eukaryotic regulatory gene."
    Hubert J.-C., Guyonvarch A., Kammerer B., Exinger F., Liljelund P., Lacroute F.
    EMBO J. 2:2071-2073(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 182-309.
  7. Cited for: SIMILARITY TO S-II.
  8. "TFIIS and strand-transfer proteins."
    Kipling D., Kearsey S.E.
    Nature 353:509-509(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTITY OF PPR2 AND DST1.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTFS2_YEAST
AccessioniPrimary (citable) accession number: P07273
Secondary accession number(s): D6VU96, P24535
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 157 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

S-II binds to RNA-polymerase II in the absence of transcription.
Present with 6260 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3