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Protein

Phosphate system positive regulatory protein PHO4

Gene

PHO4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that regulates the expression of repressible phosphatase under phosphate starvation conditions. Binds to the upstream activating sequence (UAS) of several phosphatase encoding PHO genes. Inhibited by the cyclin-CDK PHO80-PHO85 under high-phosphate conditions.

GO - Molecular functioni

  • sequence-specific DNA binding Source: SGD
  • transcription factor activity, sequence-specific DNA binding Source: SGD

GO - Biological processi

  • cellular response to phosphate starvation Source: SGD
  • chromatin remodeling Source: SGD
  • positive regulation of phosphate metabolic process Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30481-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphate system positive regulatory protein PHO4
Gene namesi
Name:PHO4
Ordered Locus Names:YFR034C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR034C.
SGDiS000001930. PHO4.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus PROSITE-ProRule annotation1 Publication

  • Note: Predominantly cytoplasmic under high-phosphate conditions and localized to the nucleus upon phosphate starvation.

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001274221 – 312Phosphate system positive regulatory protein PHO4Add BLAST312

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei100Phosphoserine; by PHO852 Publications1
Modified residuei114Phosphoserine; by PHO852 Publications1
Modified residuei128Phosphoserine; by PHO852 Publications1
Modified residuei152Phosphoserine; by PHO852 Publications1
Modified residuei204PhosphoserineCombined sources1
Modified residuei223Phosphoserine; by PHO85Combined sources2 Publications1
Modified residuei242PhosphoserineCombined sources1
Modified residuei243PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by the cyclin-CDK PHO80-PHO85 at five residues under high-phosphate conditions, preventing PHO4 from activating the structural PHO genes. Phosphorylation of Ser-114 and Ser-128 promotes nuclear export. Phosphorylation of Ser-152 decreases nuclear import. Phosphorylation of Ser-223 decreases the binding affinity for PHO2.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07270.
PRIDEiP07270.

PTM databases

iPTMnetiP07270.

Interactioni

Subunit structurei

Binds DNA as a homodimer. Interacts with transcription factor PHO2 and binds cooperatively to PHO5 UAS. Interacts with the cyclin-CDK PHO80-PHO85 and the CDK inhibitor (CKI) PHO81.2 Publications

Protein-protein interaction databases

BioGridi31191. 192 interactors.
DIPiDIP-911N.
IntActiP07270. 113 interactors.
MINTiMINT-2779852.

Structurei

Secondary structure

1312
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi254 – 258Combined sources5
Helixi260 – 275Combined sources16
Helixi279 – 282Combined sources4
Helixi294 – 306Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A0AX-ray2.80A/B251-312[»]
3W3XX-ray2.90B140-166[»]
ProteinModelPortaliP07270.
SMRiP07270.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07270.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini250 – 306bHLHPROSITE-ProRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 31Interaction with PHO80Add BLAST31
Regioni75 – 99Transcription activation domainAdd BLAST25
Regioni156 – 200Interaction with PHO80Add BLAST45
Regioni201 – 218Interaction with PHO21 PublicationAdd BLAST18
Regioni203 – 227Involved in oligomerizationAdd BLAST25

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi75 – 839aaTAD9
Motifi140 – 166Nuclear localization signalAdd BLAST27

Domaini

the 9aaTAD motif (residues 75 to 83) is a transactivation domain present in a large number of yeast and animal transcription factors.2 Publications

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP07270.
KOiK06658.
OrthoDBiEOG092C39NU.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07270-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRTTSEGIH GFVDDLEPKS SILDKVGDFI TVNTKRHDGR EDFNEQNDEL
60 70 80 90 100
NSQENHNSSE NGNENENEQD SLALDDLDRA FELVEGMDMD WMMPSHAHHS
110 120 130 140 150
PATTATIKPR LLYSPLIHTQ SAVPVTISPN LVATATSTTS ANKVTKNKSN
160 170 180 190 200
SSPYLNKRRG KPGPDSATSL FELPDSVIPT PKPKPKPKQY PKVILPSNST
210 220 230 240 250
RRVSPVTAKT SSSAEGVVVA SESPVIAPHG SSHSRSLSKR RSSGALVDDD
260 270 280 290 300
KRESHKHAEQ ARRNRLAVAL HELASLIPAE WKQQNVSAAP SKATTVEAAC
310
RYIRHLQQNV ST
Length:312
Mass (Da):34,089
Last modified:January 9, 2007 - v4
Checksum:i6DB93C0DDDB4D741
GO

Sequence cautioni

The sequence CAA27345 differs from that shown. Reason: Frameshift at position 290.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti269A → P in CAA27345 (PubMed:3008105).Curated1
Sequence conflicti269A → P (PubMed:2505053).Curated1
Sequence conflicti310V → G (PubMed:2505053).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03719 Genomic DNA. Translation: CAA27345.1. Frameshift.
D50617 Genomic DNA. Translation: BAA09273.1.
AY692776 Genomic DNA. Translation: AAT92795.1.
BK006940 Genomic DNA. Translation: DAA12477.1.
PIRiS56289.
RefSeqiNP_116692.1. NM_001179999.1.

Genome annotation databases

EnsemblFungiiBAA09273; BAA09273; BAA09273.
YFR034C; YFR034C; YFR034C.
GeneIDi850594.
KEGGisce:YFR034C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03719 Genomic DNA. Translation: CAA27345.1. Frameshift.
D50617 Genomic DNA. Translation: BAA09273.1.
AY692776 Genomic DNA. Translation: AAT92795.1.
BK006940 Genomic DNA. Translation: DAA12477.1.
PIRiS56289.
RefSeqiNP_116692.1. NM_001179999.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A0AX-ray2.80A/B251-312[»]
3W3XX-ray2.90B140-166[»]
ProteinModelPortaliP07270.
SMRiP07270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31191. 192 interactors.
DIPiDIP-911N.
IntActiP07270. 113 interactors.
MINTiMINT-2779852.

PTM databases

iPTMnetiP07270.

Proteomic databases

MaxQBiP07270.
PRIDEiP07270.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09273; BAA09273; BAA09273.
YFR034C; YFR034C; YFR034C.
GeneIDi850594.
KEGGisce:YFR034C.

Organism-specific databases

EuPathDBiFungiDB:YFR034C.
SGDiS000001930. PHO4.

Phylogenomic databases

InParanoidiP07270.
KOiK06658.
OrthoDBiEOG092C39NU.

Enzyme and pathway databases

BioCyciYEAST:G3O-30481-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP07270.
PROiP07270.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHO4_YEAST
AccessioniPrimary (citable) accession number: P07270
Secondary accession number(s): D6VTR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 171 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.