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Protein

Saccharopepsin

Gene

PEP4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Aspartyl protease implicated in the post-translational regulation of S.cerevisiae vacuolar proteinases. Acts on YSCB, on YSCY and on itself.

Catalytic activityi

Hydrolysis of proteins with broad specificity for peptide bonds. Cleaves -Leu-Leu-|-Val-Tyr- bond in a synthetic substrate. Does not act on esters of Tyr or Arg.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei109PROSITE-ProRule annotation1
Active sitei294PROSITE-ProRule annotation1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: GO_Central
  • peptidase activity Source: SGD

GO - Biological processi

  • autophagy Source: SGD
  • cellular response to starvation Source: SGD
  • CVT pathway Source: SGD
  • lysosomal microautophagy Source: SGD
  • proteolysis involved in cellular protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BioCyciYEAST:YPL154C-MONOMER.
BRENDAi3.4.23.25. 984.
ReactomeiR-SCE-2132295. MHC class II antigen presentation.
R-SCE-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiA01.018.

Names & Taxonomyi

Protein namesi
Recommended name:
Saccharopepsin (EC:3.4.23.25)
Alternative name(s):
Aspartate protease
Short name:
PrA
Short name:
Proteinase A
Carboxypeptidase Y-deficient protein 4
Proteinase YSCA
Gene namesi
Name:PEP4
Synonyms:PHO9, PRA1
Ordered Locus Names:YPL154C
ORF Names:P2585
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL154C.
SGDiS000006075. PEP4.

Subcellular locationi

  • Vacuole

  • Note: Lysosome-like vacuoles.

GO - Cellular componenti

  • fungal-type vacuole Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi294D → A: Inactivation. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL4451.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
PropeptideiPRO_000002587323 – 76Activation peptide1 PublicationAdd BLAST54
ChainiPRO_000002587477 – 405SaccharopepsinAdd BLAST329

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi122 ↔ 127
Glycosylationi144N-linked (GlcNAc...)1
Disulfide bondi328 ↔ 361
Glycosylationi345N-linked (GlcNAc...)1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP07267.
PRIDEiP07267.

2D gel databases

UCD-2DPAGEP07267.

Miscellaneous databases

PMAP-CutDBP07267.

Interactioni

Protein-protein interaction databases

BioGridi36029. 50 interactors.
DIPiDIP-4442N.
IntActiP07267. 8 interactors.
MINTiMINT-382859.

Chemistry databases

BindingDBiP07267.

Structurei

Secondary structure

1405
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi79 – 82Combined sources4
Beta strandi84 – 86Combined sources3
Turni87 – 89Combined sources3
Beta strandi90 – 97Combined sources8
Turni98 – 101Combined sources4
Beta strandi102 – 109Combined sources8
Beta strandi115 – 119Combined sources5
Helixi125 – 128Combined sources4
Helixi135 – 137Combined sources3
Beta strandi142 – 152Combined sources11
Beta strandi155 – 168Combined sources14
Beta strandi171 – 183Combined sources13
Helixi186 – 189Combined sources4
Beta strandi195 – 199Combined sources5
Helixi203 – 205Combined sources3
Helixi207 – 209Combined sources3
Helixi213 – 219Combined sources7
Beta strandi224 – 232Combined sources9
Helixi235 – 237Combined sources3
Beta strandi240 – 249Combined sources10
Helixi252 – 254Combined sources3
Beta strandi255 – 263Combined sources9
Turni267 – 270Combined sources4
Beta strandi271 – 279Combined sources9
Beta strandi282 – 285Combined sources4
Beta strandi290 – 293Combined sources4
Beta strandi300 – 302Combined sources3
Helixi304 – 314Combined sources11
Beta strandi317 – 319Combined sources3
Beta strandi322 – 326Combined sources5
Helixi328 – 333Combined sources6
Beta strandi337 – 341Combined sources5
Beta strandi344 – 348Combined sources5
Turni350 – 352Combined sources3
Beta strandi353 – 357Combined sources5
Beta strandi360 – 363Combined sources4
Beta strandi365 – 367Combined sources3
Turni372 – 374Combined sources3
Beta strandi376 – 380Combined sources5
Helixi382 – 385Combined sources4
Beta strandi388 – 393Combined sources6
Turni394 – 397Combined sources4
Beta strandi398 – 404Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DP5X-ray2.20A77-405[»]
1DPJX-ray1.80A77-405[»]
1FMUX-ray2.70A77-405[»]
1FMXX-ray2.61A/B77-405[»]
1FQ4X-ray2.70A77-405[»]
1FQ5X-ray2.40A77-405[»]
1FQ6X-ray2.70A77-405[»]
1FQ7X-ray2.80A77-405[»]
1FQ8X-ray2.80A77-405[»]
1G0VX-ray2.00A77-405[»]
2JXRX-ray2.40A77-405[»]
ProteinModelPortaliP07267.
SMRiP07267.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07267.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini91 – 402Peptidase A1PROSITE-ProRule annotationAdd BLAST312

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
InParanoidiP07267.
KOiK01381.
OMAiYTHNIDA.
OrthoDBiEOG092C33N0.

Family and domain databases

CDDicd05488. Proteinase_A_fungi. 1 hit.
Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR033819. Saccharopepsin.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 2 hits.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 2 hits.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07267-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSLKALLPL ALLLVSANQV AAKVHKAKIY KHELSDEMKE VTFEQHLAHL
60 70 80 90 100
GQKYLTQFEK ANPEVVFSRE HPFFTEGGHD VPLTNYLNAQ YYTDITLGTP
110 120 130 140 150
PQNFKVILDT GSSNLWVPSN ECGSLACFLH SKYDHEASSS YKANGTEFAI
160 170 180 190 200
QYGTGSLEGY ISQDTLSIGD LTIPKQDFAE ATSEPGLTFA FGKFDGILGL
210 220 230 240 250
GYDTISVDKV VPPFYNAIQQ DLLDEKRFAF YLGDTSKDTE NGGEATFGGI
260 270 280 290 300
DESKFKGDIT WLPVRRKAYW EVKFEGIGLG DEYAELESHG AAIDTGTSLI
310 320 330 340 350
TLPSGLAEMI NAEIGAKKGW TGQYTLDCNT RDNLPDLIFN FNGYNFTIGP
360 370 380 390 400
YDYTLEVSGS CISAITPMDF PEPVGPLAIV GDAFLRKYYS IYDLGNNAVG

LAKAI
Length:405
Mass (Da):44,499
Last modified:April 1, 1988 - v1
Checksum:iB0AA36BA098D2BF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13358 Genomic DNA. Translation: AAB63975.1.
X96770 Genomic DNA. Translation: CAA65567.1.
Z73510 Genomic DNA. Translation: CAA97859.1.
Z11963 Genomic DNA. Translation: CAA78020.1.
BK006949 Genomic DNA. Translation: DAA11280.1.
PIRiA25379.
RefSeqiNP_015171.1. NM_001183968.1.

Genome annotation databases

EnsemblFungiiYPL154C; YPL154C; YPL154C.
GeneIDi855949.
KEGGisce:YPL154C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13358 Genomic DNA. Translation: AAB63975.1.
X96770 Genomic DNA. Translation: CAA65567.1.
Z73510 Genomic DNA. Translation: CAA97859.1.
Z11963 Genomic DNA. Translation: CAA78020.1.
BK006949 Genomic DNA. Translation: DAA11280.1.
PIRiA25379.
RefSeqiNP_015171.1. NM_001183968.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DP5X-ray2.20A77-405[»]
1DPJX-ray1.80A77-405[»]
1FMUX-ray2.70A77-405[»]
1FMXX-ray2.61A/B77-405[»]
1FQ4X-ray2.70A77-405[»]
1FQ5X-ray2.40A77-405[»]
1FQ6X-ray2.70A77-405[»]
1FQ7X-ray2.80A77-405[»]
1FQ8X-ray2.80A77-405[»]
1G0VX-ray2.00A77-405[»]
2JXRX-ray2.40A77-405[»]
ProteinModelPortaliP07267.
SMRiP07267.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36029. 50 interactors.
DIPiDIP-4442N.
IntActiP07267. 8 interactors.
MINTiMINT-382859.

Chemistry databases

BindingDBiP07267.
ChEMBLiCHEMBL4451.

Protein family/group databases

MEROPSiA01.018.

2D gel databases

UCD-2DPAGEP07267.

Proteomic databases

MaxQBiP07267.
PRIDEiP07267.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL154C; YPL154C; YPL154C.
GeneIDi855949.
KEGGisce:YPL154C.

Organism-specific databases

EuPathDBiFungiDB:YPL154C.
SGDiS000006075. PEP4.

Phylogenomic databases

GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
InParanoidiP07267.
KOiK01381.
OMAiYTHNIDA.
OrthoDBiEOG092C33N0.

Enzyme and pathway databases

BioCyciYEAST:YPL154C-MONOMER.
BRENDAi3.4.23.25. 984.
ReactomeiR-SCE-2132295. MHC class II antigen presentation.
R-SCE-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiP07267.
PMAP-CutDBP07267.
PROiP07267.

Family and domain databases

CDDicd05488. Proteinase_A_fungi. 1 hit.
Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR033819. Saccharopepsin.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 2 hits.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 2 hits.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCARP_YEAST
AccessioniPrimary (citable) accession number: P07267
Secondary accession number(s): D6W3L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 30, 2016
This is version 182 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.