Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-isopropylmalate dehydratase

Gene

LEU1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (LEU4), 2-isopropylmalate synthase 2, mitochondrial (LEU9)
  2. 3-isopropylmalate dehydratase (LEU1)
  3. 3-isopropylmalate dehydrogenase (LEU2)
  4. Branched-chain-amino-acid aminotransferase, mitochondrial (BAT1), Branched-chain-amino-acid aminotransferase, cytosolic (BAT2)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi360Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi421Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi424Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

  • 3-isopropylmalate dehydratase activity Source: SGD
  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • branched-chain amino acid biosynthetic process Source: UniProtKB-KW
  • leucine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YGL009C-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase (EC:4.2.1.33)
Alternative name(s):
Alpha-IPM isomerase
Short name:
IPMI
Isopropylmalate isomerase
Gene namesi
Name:LEU1
Ordered Locus Names:YGL009C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL009C.
SGDiS000002977. LEU1.

Subcellular locationi

GO - Cellular componenti

  • 3-isopropylmalate dehydratase complex Source: InterPro
  • cytoplasmic stress granule Source: SGD
  • cytosol Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000768941 – 7793-isopropylmalate dehydrataseAdd BLAST779

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei488PhosphoserineCombined sources1
Modified residuei494PhosphothreonineCombined sources1
Modified residuei495PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07264.
PRIDEiP07264.

PTM databases

iPTMnetiP07264.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi33237. 61 interactors.
DIPiDIP-6715N.
IntActiP07264. 10 interactors.
MINTiMINT-618120.

Structurei

3D structure databases

ProteinModelPortaliP07264.
SMRiP07264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family.Curated

Phylogenomic databases

GeneTreeiENSGT00730000114222.
HOGENOMiHOG000226972.
InParanoidiP07264.
KOiK01702.
OMAiGCVIEFA.
OrthoDBiEOG092C0T13.

Family and domain databases

CDDicd01583. IPMI. 1 hit.
cd01577. IPMI_Swivel. 1 hit.
Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1. 1 hit.
MF_01031. LeuD_type1. 1 hit.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR004431. 3-IsopropMal_deHydase_ssu.
IPR012235. 3-IsopropMal_deHydtase_ssu/lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
IPR033941. IPMI_cat.
IPR033940. IPMI_Swivel.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 2 hits.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001418. ACN. 1 hit.
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
TIGR00171. leuD. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07264-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVYTPSKGPR TLYDKVFDAH VVHQDENGSF LLYIDRHLVH EVTSPQAFEG
60 70 80 90 100
LENAGRKVRR VDCTLATVDH NIPTESRKNF KSLDTFIKQT DSRLQVKTLE
110 120 130 140 150
NNVKQFGVPY FGMSDARQGI VHTIGPEEGF TLPGTTVVCG DSHTSTHGAF
160 170 180 190 200
GSLAFGIGTS EVEHVLATQT IIQAKSKNMR ITVNGKLSPG ITSKDLILYI
210 220 230 240 250
IGLIGTAGGT GCVIEFAGEA IEALSMEARM SMCNMAIEAG ARAGMIKPDE
260 270 280 290 300
TTFQYTKGRP LAPKGAEWEK AVAYWKTLKT DEGAKFDHEI NIEAVDVIPT
310 320 330 340 350
ITWGTSPQDA LPITGSVPDP KNVTDPIKKS GMERALAYMG LEPNTPLKSI
360 370 380 390 400
KVDKVFIGSC TNGRIEDLRS AAAVVRGQKL ASNIKLAMVV PGSGLVKKQA
410 420 430 440 450
EAEGLDKIFQ EAGFEWREAG CSICLGMNPD ILDAYERCAS TSNRNFEGRQ
460 470 480 490 500
GALSRTHLMS PAMAAAAGIA GHFVDIREFE YKDQDQSSPK VEVTSEDEKE
510 520 530 540 550
LESAAYDHAE PVQPEDAPQD IANDELKDIP VKSDDTPAKP SSSGMKPFLT
560 570 580 590 600
LEGISAPLDK ANVDTDAIIP KQFLKTIKRT GLKKGLFYEW RFRKDDQGKD
610 620 630 640 650
QETDFVLNVE PWREAEILVV TGDNFGCGSS REHAPWALKD FGIKSIIAPS
660 670 680 690 700
YGDIFYNNSF KNGLLPIRLD QQIIIDKLIP IANKGGKLCV DLPNQKILDS
710 720 730 740 750
DGNVLVDHFE IEPFRKHCLV NGLDDIGITL QKEEYISRYE ALRREKYSFL
760 770
EGGSKLLKFD NVPKRKAVTT TFDKVHQDW
Length:779
Mass (Da):85,794
Last modified:October 1, 1996 - v3
Checksum:iBD409A9702AE3E57
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti291N → TLKH in AAB19612 (PubMed:1840714).Curated1
Sequence conflicti423I → M in AAB19612 (PubMed:1840714).Curated1
Sequence conflicti459M → I in AAB19612 (PubMed:1840714).Curated1
Sequence conflicti744R → K in AAB19612 (PubMed:1840714).Curated1
Sequence conflicti744R → K in S58126 (PubMed:1882553).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S57886 Genomic DNA. Translation: AAB19612.1.
Z72531 Genomic DNA. Translation: CAA96709.1.
K01969 Genomic DNA. Translation: AAA34742.1.
S58126 Genomic DNA. No translation available.
BK006941 Genomic DNA. Translation: DAA08089.1.
PIRiS64011.
RefSeqiNP_011506.1. NM_001180874.1.

Genome annotation databases

EnsemblFungiiYGL009C; YGL009C; YGL009C.
GeneIDi852875.
KEGGisce:YGL009C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S57886 Genomic DNA. Translation: AAB19612.1.
Z72531 Genomic DNA. Translation: CAA96709.1.
K01969 Genomic DNA. Translation: AAA34742.1.
S58126 Genomic DNA. No translation available.
BK006941 Genomic DNA. Translation: DAA08089.1.
PIRiS64011.
RefSeqiNP_011506.1. NM_001180874.1.

3D structure databases

ProteinModelPortaliP07264.
SMRiP07264.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33237. 61 interactors.
DIPiDIP-6715N.
IntActiP07264. 10 interactors.
MINTiMINT-618120.

PTM databases

iPTMnetiP07264.

Proteomic databases

MaxQBiP07264.
PRIDEiP07264.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL009C; YGL009C; YGL009C.
GeneIDi852875.
KEGGisce:YGL009C.

Organism-specific databases

EuPathDBiFungiDB:YGL009C.
SGDiS000002977. LEU1.

Phylogenomic databases

GeneTreeiENSGT00730000114222.
HOGENOMiHOG000226972.
InParanoidiP07264.
KOiK01702.
OMAiGCVIEFA.
OrthoDBiEOG092C0T13.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.
BioCyciYEAST:YGL009C-MONOMER.

Miscellaneous databases

PROiP07264.

Family and domain databases

CDDicd01583. IPMI. 1 hit.
cd01577. IPMI_Swivel. 1 hit.
Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1. 1 hit.
MF_01031. LeuD_type1. 1 hit.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR004431. 3-IsopropMal_deHydase_ssu.
IPR012235. 3-IsopropMal_deHydtase_ssu/lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
IPR033941. IPMI_cat.
IPR033940. IPMI_Swivel.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 2 hits.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001418. ACN. 1 hit.
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
TIGR00171. leuD. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEUC_YEAST
AccessioniPrimary (citable) accession number: P07264
Secondary accession number(s): D6VUC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 167 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 96300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.