Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Histidine--tRNA ligase, mitochondrial

Gene

HTS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the aminoacylation of histidyl-tRNA in both the cytoplasm and the mitochondrion.2 Publications

Catalytic activityi

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).

GO - Molecular functioni

  • aminoacyl-tRNA ligase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • histidine-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  • histidyl-tRNA aminoacylation Source: SGD
  • mitochondrial translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34192-MONOMER.
BRENDAi6.1.1.21. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine--tRNA ligase, mitochondrial (EC:6.1.1.21)
Alternative name(s):
Histidyl-tRNA synthetase
Short name:
HisRS
Gene namesi
Name:HTS1
Ordered Locus Names:YPR033C
ORF Names:YP9367.13C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR033C.
SGDiS000006237. HTS1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2020MitochondrionAdd
BLAST
Chaini21 – 546526Histidine--tRNA ligase, mitochondrialPRO_0000035804Add
BLAST

Proteomic databases

MaxQBiP07263.
PeptideAtlasiP07263.
PRIDEiP07263.

Interactioni

Protein-protein interaction databases

BioGridi36211. 27 interactions.
DIPiDIP-3909N.
IntActiP07263. 5 interactions.
MINTiMINT-487669.

Structurei

3D structure databases

ProteinModelPortaliP07263.
SMRiP07263. Positions 55-503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000005922.
HOGENOMiHOG000018075.
InParanoidiP07263.
KOiK01892.
OMAiIACMHEE.
OrthoDBiEOG751NQG.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
[Graphical view]
PANTHERiPTHR11476. PTHR11476. 2 hits.
PfamiPF03129. HGTP_anticodon. 1 hit.
[Graphical view]
PIRSFiPIRSF001549. His-tRNA_synth. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Mitochondrial (identifier: P07263-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSRSLNKVV TSIKSSSIIR MSSATAAATS APTANAANAL KASKAPKKGK
60 70 80 90 100
LQVSLKTPKG TKDWADSDMV IREAIFSTLS GLFKKHGGVT IDTPVFELRE
110 120 130 140 150
ILAGKYGEDS KLIYNLEDQG GELCSLRYDL TVPFARYVAM NNIQSIKRYH
160 170 180 190 200
IAKVYRRDQP AMTKGRMREF YQCDFDVAGT FESMVPDSEC LSILVEGLTS
210 220 230 240 250
LGIKDFKIKL NHRKILDGIF QIAGVKDEDV RKISSAVDKL DKSPWEAVKK
260 270 280 290 300
EMTEEKGQSE ETADKIGEYV KLNGSLKEIH AVLSADANIT SNEKAKQGLD
310 320 330 340 350
DIATLMKYTE AFDIDSFISF DLSLARGLDY YTGLIYEVVT SASAPPENAS
360 370 380 390 400
ELKKKAKSAE DASEFVGVGS IAAGGRYDNL VNMFSEASGK KSTQIPCVGI
410 420 430 440 450
SFGVERIFSL IKQRINSSTT IKPTATQVFV MAFGGGKDWT GYLPERMKVT
460 470 480 490 500
KQLWDAGIEA EYVYKAKANP RKQFDAAEKA GCHIAVILGK EEYLEGKLRV
510 520 530 540
KRLGQEFADD DGELVSAADI VPIVQEKLSQ IHEDGLNEVT RLIKGL
Length:546
Mass (Da):59,953
Last modified:November 1, 1997 - v2
Checksum:i8BDCCEAB4DD73973
GO
Isoform Cytoplasmic (identifier: P07263-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Note: Produced by alternative initiation at Met-21 of isoform Mitochondrial.
Show »
Length:526
Mass (Da):57,751
Checksum:iE150FF5C2B5001C7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti476 – 4783AAE → TTK in AAA34695 (PubMed:3521891).Curated
Sequence conflicti476 – 4783AAE → TTK in AAA34696 (PubMed:3521891).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2020Missing in isoform Cytoplasmic. CuratedVSP_018907Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14048 Genomic DNA. Translation: AAA34695.1.
M14048 Genomic DNA. Translation: AAA34696.1.
Z71255 Genomic DNA. Translation: CAA94983.1.
Z49274 Genomic DNA. Translation: CAA89287.1.
AY692751 Genomic DNA. Translation: AAT92770.1.
BK006949 Genomic DNA. Translation: DAA11459.1.
PIRiS54507. SYBYHM.
RefSeqiNP_015358.1. NM_001184130.1. [P07263-1]

Genome annotation databases

EnsemblFungiiYPR033C; YPR033C; YPR033C. [P07263-1]
GeneIDi856145.
KEGGisce:YPR033C.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14048 Genomic DNA. Translation: AAA34695.1.
M14048 Genomic DNA. Translation: AAA34696.1.
Z71255 Genomic DNA. Translation: CAA94983.1.
Z49274 Genomic DNA. Translation: CAA89287.1.
AY692751 Genomic DNA. Translation: AAT92770.1.
BK006949 Genomic DNA. Translation: DAA11459.1.
PIRiS54507. SYBYHM.
RefSeqiNP_015358.1. NM_001184130.1. [P07263-1]

3D structure databases

ProteinModelPortaliP07263.
SMRiP07263. Positions 55-503.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36211. 27 interactions.
DIPiDIP-3909N.
IntActiP07263. 5 interactions.
MINTiMINT-487669.

Proteomic databases

MaxQBiP07263.
PeptideAtlasiP07263.
PRIDEiP07263.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR033C; YPR033C; YPR033C. [P07263-1]
GeneIDi856145.
KEGGisce:YPR033C.

Organism-specific databases

EuPathDBiFungiDB:YPR033C.
SGDiS000006237. HTS1.

Phylogenomic databases

GeneTreeiENSGT00390000005922.
HOGENOMiHOG000018075.
InParanoidiP07263.
KOiK01892.
OMAiIACMHEE.
OrthoDBiEOG751NQG.

Enzyme and pathway databases

BioCyciYEAST:G3O-34192-MONOMER.
BRENDAi6.1.1.21. 984.

Miscellaneous databases

NextBioi981265.
PROiP07263.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
[Graphical view]
PANTHERiPTHR11476. PTHR11476. 2 hits.
PfamiPF03129. HGTP_anticodon. 1 hit.
[Graphical view]
PIRSFiPIRSF001549. His-tRNA_synth. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The HTS1 gene encodes both the cytoplasmic and mitochondrial histidine tRNA synthetases of S. cerevisiae."
    Natsoulis G., Hilger F., Fink G.R.
    Cell 46:235-243(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ALTERNATIVE INITIATION.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "HTS1 encodes both the cytoplasmic and mitochondrial histidyl-tRNA synthetase of Saccharomyces cerevisiae: mutations alter the specificity of compartmentation."
    Chiu M.I., Mason T.L., Fink G.R.
    Genetics 132:987-1001(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ALTERNATIVE INITIATION, SUBCELLULAR LOCATION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYH_YEAST
AccessioniPrimary (citable) accession number: P07263
Secondary accession number(s): D6W443, Q12095
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 164 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 26800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.