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Protein

Histidine--tRNA ligase, mitochondrial

Gene

HTS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the aminoacylation of histidyl-tRNA in both the cytoplasm and the mitochondrion.2 Publications

Miscellaneous

Present with 26800 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei156L-histidineBy similarity1
Binding sitei172L-histidineBy similarity1
Binding sitei176L-histidineBy similarity1
Binding sitei326L-histidineBy similarity1

GO - Molecular functioni

  • aminoacyl-tRNA ligase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • histidine-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  • histidyl-tRNA aminoacylation Source: SGD
  • mitochondrial translation Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34192-MONOMER
BRENDAi6.1.1.21 984

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine--tRNA ligase, mitochondrial (EC:6.1.1.21)
Alternative name(s):
Histidyl-tRNA synthetase
Short name:
HisRS
Gene namesi
Name:HTS1
Ordered Locus Names:YPR033C
ORF Names:YP9367.13C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR033C
SGDiS000006237 HTS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Lethal.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi198 – 207Missing : Does not rescue the lethal phenotype of the deletion mutant. 1 Publication10
Mutagenesisi198L → V: Rescues the lethal phenotype of the deletion mutant. 1 Publication1
Mutagenesisi381V → L: Partially rescues the lethal phenotype of the deletion mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 20MitochondrionAdd BLAST20
ChainiPRO_000003580421 – 546Histidine--tRNA ligase, mitochondrialAdd BLAST526

Proteomic databases

PaxDbiP07263
PeptideAtlasiP07263
PRIDEiP07263

Interactioni

Protein-protein interaction databases

BioGridi36211, 152 interactors
DIPiDIP-3909N
IntActiP07263, 6 interactors
MINTiP07263
STRINGi4932.YPR033C

Structurei

3D structure databases

ProteinModelPortaliP07263
SMRiP07263
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 131L-histidine bindingBy similarity3
Regioni330 – 331L-histidine bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000005922
HOGENOMiHOG000018075
InParanoidiP07263
KOiK01892
OMAiCDFDFIG
OrthoDBiEOG092C1ISK

Family and domain databases

CDDicd00859 HisRS_anticodon, 1 hit
Gene3Di3.40.50.800, 1 hit
InterProiView protein in InterPro
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR015807 His-tRNA-ligase
IPR004516 HisRS/HisZ
IPR033656 HisRS_anticodon
PANTHERiPTHR11476 PTHR11476, 1 hit
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PIRSFiPIRSF001549 His-tRNA_synth, 1 hit
TIGRFAMsiTIGR00442 hisS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Mitochondrial (identifier: P07263-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSRSLNKVV TSIKSSSIIR MSSATAAATS APTANAANAL KASKAPKKGK
60 70 80 90 100
LQVSLKTPKG TKDWADSDMV IREAIFSTLS GLFKKHGGVT IDTPVFELRE
110 120 130 140 150
ILAGKYGEDS KLIYNLEDQG GELCSLRYDL TVPFARYVAM NNIQSIKRYH
160 170 180 190 200
IAKVYRRDQP AMTKGRMREF YQCDFDVAGT FESMVPDSEC LSILVEGLTS
210 220 230 240 250
LGIKDFKIKL NHRKILDGIF QIAGVKDEDV RKISSAVDKL DKSPWEAVKK
260 270 280 290 300
EMTEEKGQSE ETADKIGEYV KLNGSLKEIH AVLSADANIT SNEKAKQGLD
310 320 330 340 350
DIATLMKYTE AFDIDSFISF DLSLARGLDY YTGLIYEVVT SASAPPENAS
360 370 380 390 400
ELKKKAKSAE DASEFVGVGS IAAGGRYDNL VNMFSEASGK KSTQIPCVGI
410 420 430 440 450
SFGVERIFSL IKQRINSSTT IKPTATQVFV MAFGGGKDWT GYLPERMKVT
460 470 480 490 500
KQLWDAGIEA EYVYKAKANP RKQFDAAEKA GCHIAVILGK EEYLEGKLRV
510 520 530 540
KRLGQEFADD DGELVSAADI VPIVQEKLSQ IHEDGLNEVT RLIKGL
Length:546
Mass (Da):59,953
Last modified:November 1, 1997 - v2
Checksum:i8BDCCEAB4DD73973
GO
Isoform Cytoplasmic (identifier: P07263-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Note: Produced by alternative initiation at Met-21 of isoform Mitochondrial.
Show »
Length:526
Mass (Da):57,751
Checksum:iE150FF5C2B5001C7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti476 – 478AAE → TTK in AAA34695 (PubMed:3521891).Curated3
Sequence conflicti476 – 478AAE → TTK in AAA34696 (PubMed:3521891).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0189071 – 20Missing in isoform Cytoplasmic. CuratedAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14048 Genomic DNA Translation: AAA34695.1
M14048 Genomic DNA Translation: AAA34696.1
Z71255 Genomic DNA Translation: CAA94983.1
Z49274 Genomic DNA Translation: CAA89287.1
AY692751 Genomic DNA Translation: AAT92770.1
BK006949 Genomic DNA Translation: DAA11459.1
PIRiS54507 SYBYHM
RefSeqiNP_015358.1, NM_001184130.1 [P07263-1]

Genome annotation databases

EnsemblFungiiYPR033C; YPR033C; YPR033C [P07263-1]
GeneIDi856145
KEGGisce:YPR033C

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiSYH_YEAST
AccessioniPrimary (citable) accession number: P07263
Secondary accession number(s): D6W443, Q12095
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 180 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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