P07263 (SYH_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histidine--tRNA ligase, mitochondrial
EC=6.1.1.21

Alternative name(s):
Histidyl-tRNA synthetase
Short name=HisRS

Gene names

Name:	HTS1
Ordered Locus Names:	YPR033C
ORF Names:	YP9367.13C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	546 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).
Subcellular location	Isoform Cytoplasmic: Cytoplasm. Isoform Mitochondrial: Mitochondrion.
Miscellaneous	Present with 26800 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm Mitochondrion
Coding sequence diversity	Alternative initiation
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histidyl-tRNA aminoacylation Inferred from direct assay. Source: SGD mitochondrial translation Inferred from genetic interaction. Source: SGD
Cellular component	mitochondrion Inferred from mutant phenotype Ref.1. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW histidine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AKL1	P38080	1	EBI-18711,EBI-9528

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Mitochondrial (identifier: P07263-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Cytoplasmic (identifier: P07263-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Note: Produced by alternative initiation at Met-21 of isoform Mitochondrial.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 20	20	Mitochondrion
	Chain	21 – 546	526	Histidine--tRNA ligase, mitochondrial		PRO_0000035804
Natural variations
	Alternative sequence	1 – 20	20	Missing in isoform Cytoplasmic.		VSP_018907
Experimental info
	Sequence conflict	476 – 478	3	AAE → TTK in AAA34695. Ref.1
	Sequence conflict	476 – 478	3	AAE → TTK in AAA34696. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Mitochondrial [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 8BDCCEAB4DD73973	FASTA	546	59,953
	10 20 30 40 50 60 MLSRSLNKVV TSIKSSSIIR MSSATAAATS APTANAANAL KASKAPKKGK LQVSLKTPKG 70 80 90 100 110 120 TKDWADSDMV IREAIFSTLS GLFKKHGGVT IDTPVFELRE ILAGKYGEDS KLIYNLEDQG 130 140 150 160 170 180 GELCSLRYDL TVPFARYVAM NNIQSIKRYH IAKVYRRDQP AMTKGRMREF YQCDFDVAGT 190 200 210 220 230 240 FESMVPDSEC LSILVEGLTS LGIKDFKIKL NHRKILDGIF QIAGVKDEDV RKISSAVDKL 250 260 270 280 290 300 DKSPWEAVKK EMTEEKGQSE ETADKIGEYV KLNGSLKEIH AVLSADANIT SNEKAKQGLD 310 320 330 340 350 360 DIATLMKYTE AFDIDSFISF DLSLARGLDY YTGLIYEVVT SASAPPENAS ELKKKAKSAE 370 380 390 400 410 420 DASEFVGVGS IAAGGRYDNL VNMFSEASGK KSTQIPCVGI SFGVERIFSL IKQRINSSTT 430 440 450 460 470 480 IKPTATQVFV MAFGGGKDWT GYLPERMKVT KQLWDAGIEA EYVYKAKANP RKQFDAAEKA 490 500 510 520 530 540 GCHIAVILGK EEYLEGKLRV KRLGQEFADD DGELVSAADI VPIVQEKLSQ IHEDGLNEVT RLIKGL
	Isoform Cytoplasmic [UniParc]. Checksum: E150FF5C2B5001C7	FASTA	526	57,751
	10 20 30 40 50 60 MSSATAAATS APTANAANAL KASKAPKKGK LQVSLKTPKG TKDWADSDMV IREAIFSTLS 70 80 90 100 110 120 GLFKKHGGVT IDTPVFELRE ILAGKYGEDS KLIYNLEDQG GELCSLRYDL TVPFARYVAM 130 140 150 160 170 180 NNIQSIKRYH IAKVYRRDQP AMTKGRMREF YQCDFDVAGT FESMVPDSEC LSILVEGLTS 190 200 210 220 230 240 LGIKDFKIKL NHRKILDGIF QIAGVKDEDV RKISSAVDKL DKSPWEAVKK EMTEEKGQSE 250 260 270 280 290 300 ETADKIGEYV KLNGSLKEIH AVLSADANIT SNEKAKQGLD DIATLMKYTE AFDIDSFISF 310 320 330 340 350 360 DLSLARGLDY YTGLIYEVVT SASAPPENAS ELKKKAKSAE DASEFVGVGS IAAGGRYDNL 370 380 390 400 410 420 VNMFSEASGK KSTQIPCVGI SFGVERIFSL IKQRINSSTT IKPTATQVFV MAFGGGKDWT 430 440 450 460 470 480 GYLPERMKVT KQLWDAGIEA EYVYKAKANP RKQFDAAEKA GCHIAVILGK EEYLEGKLRV 490 500 510 520 KRLGQEFADD DGELVSAADI VPIVQEKLSQ IHEDGLNEVT RLIKGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The HTS1 gene encodes both the cytoplasmic and mitochondrial histidine tRNA synthetases of S. cerevisiae." Natsoulis G., Hilger F., Fink G.R. Cell 46:235-243(1986) [PubMed: 3521891] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"HTS1 encodes both the cytoplasmic and mitochondrial histidyl-tRNA synthetase of Saccharomyces cerevisiae: mutations alter the specificity of compartmentation." Chiu M.I., Mason T.L., Fink G.R. Genetics 132:987-1001(1992) [PubMed: 1459448] [Abstract] Cited for: MUTAGENESIS.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M14048 Genomic DNA. Translation: AAA34695.1. M14048 Genomic DNA. Translation: AAA34696.1. Z71255 Genomic DNA. Translation: CAA94983.1. Z49274 Genomic DNA. Translation: CAA89287.1. AY692751 Genomic DNA. Translation: AAT92770.1. BK006949 Genomic DNA. Translation: DAA11459.1.
PIR	SYBYHM. S54507.
RefSeq	NP_015358.1. NM_001184130.1.
3D structure databases
ProteinModelPortal	P07263.
SMR	P07263. Positions 53-528.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3909N.
IntAct	P07263. 5 interactions.
MINT	MINT-487669.
STRING	P07263.
Proteomic databases
PeptideAtlas	P07263.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPR033C; YPR033C; YPR033C.
GeneID	856145.
KEGG	sce:YPR033C.
NMPDR	fig|4932.3.peg.6493.
Organism-specific databases
CYGD	YPR033c.
SGD	S000006237. HTS1.
Phylogenomic databases
eggNOG	fuNOG05053.
GeneTree	EFGT00070000008727.
HOGENOM	HBG616575.
OMA	LVSELWD.
OrthoDB	EOG4JQ75X.
Enzyme and pathway databases
BRENDA	6.1.1.21. 984.
Gene expression databases
ArrayExpress	P07263.
Genevestigator	P07263.
GermOnline	YPR033C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR015807. His-tRNA-synth_IIa_subgr. IPR004516. His-tRNA_synth_IIA. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KO	K01892.
PANTHER	PTHR11476. His-tRNA_synth. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001549. His-tRNA_synth. 1 hit.
SUPFAM	SSF52954. Anticodon_bd. 1 hit.
TIGRFAMs	TIGR00442. HisS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981265.

Entry information

Entry name

SYH_YEAST

Accession

Primary (citable) accession number: P07263
Secondary accession number(s): D6W443, Q12095

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 129 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program