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Protein

NADP-specific glutamate dehydrogenase 1

Gene

GDH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 77500 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei110PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi174 – 203NADBy similarityAdd BLAST30

GO - Molecular functioni

  • glutamate dehydrogenase (NADP+) activity Source: SGD

GO - Biological processi

  • ammonia assimilation cycle Source: SGD
  • glutamate biosynthetic process Source: SGD

Keywordsi

Molecular functionOxidoreductase
LigandNADP

Enzyme and pathway databases

BioCyciMetaCyc:YOR375C-MONOMER
YEAST:YOR375C-MONOMER
ReactomeiR-SCE-2151201 Transcriptional activation of mitochondrial biogenesis
R-SCE-70614 Amino acid synthesis and interconversion (transamination)

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-specific glutamate dehydrogenase 1 (EC:1.4.1.4)
Short name:
NADP-GDH 1
Alternative name(s):
NADP-dependent glutamate dehydrogenase 1
Gene namesi
Name:GDH1
Synonyms:URE1
Ordered Locus Names:YOR375C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR375C
SGDiS000005902 GDH1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001827982 – 454NADP-specific glutamate dehydrogenase 1Add BLAST453

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Cross-linki325Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki371Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki433Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP07262
PaxDbiP07262
PRIDEiP07262

PTM databases

iPTMnetiP07262

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

BioGridi34758, 75 interactors
DIPiDIP-1329N
IntActiP07262, 53 interactors
MINTiP07262
STRINGi4932.YOR375C

Structurei

3D structure databases

ProteinModelPortaliP07262
SMRiP07262
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000000854
HOGENOMiHOG000243799
InParanoidiP07262
KOiK00262
OMAiPCFAAFP
OrthoDBiEOG092C2DRP

Family and domain databases

CDDicd05313 NAD_bind_2_Glu_DH, 1 hit
InterProiView protein in InterPro
IPR006095 Glu/Leu/Phe/Val_DH
IPR033524 Glu/Leu/Phe/Val_DH_AS
IPR006096 Glu/Leu/Phe/Val_DH_C
IPR006097 Glu/Leu/Phe/Val_DH_dimer_dom
IPR014362 Glu_DH
IPR036291 NAD(P)-bd_dom_sf
IPR033922 NAD_bind_Glu_DH
PfamiView protein in Pfam
PF00208 ELFV_dehydrog, 1 hit
PF02812 ELFV_dehydrog_N, 1 hit
PIRSFiPIRSF000185 Glu_DH, 1 hit
PRINTSiPR00082 GLFDHDRGNASE
SMARTiView protein in SMART
SM00839 ELFV_dehydrog, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00074 GLFV_DEHYDROGENASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07262-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPEFQQAY EEVVSSLEDS TLFEQHPEYR KVLPIVSVPE RIIQFRVTWE
60 70 80 90 100
NDKGEQEVAQ GYRVQYNSAK GPYKGGLRFH PSVNLSILKF LGFEQIFKNS
110 120 130 140 150
LTGLDMGGGK GGLCVDLKGR SNNEIRRICY AFMRELSRHI GQDTDVPAGD
160 170 180 190 200
IGVGGREIGY LFGAYRSYKN SWEGVLTGKG LNWGGSLIRP EATGYGLVYY
210 220 230 240 250
TQAMIDYATN GKESFEGKRV TISGSGNVAQ YAALKVIELG GTVVSLSDSK
260 270 280 290 300
GCIISETGIT SEQVADISSA KVNFKSLEQI VNEYSTFSEN KVQYIAGARP
310 320 330 340 350
WTHVQKVDIA LPCATQNEVS GEEAKALVAQ GVKFIAEGSN MGSTPEAIAV
360 370 380 390 400
FETARSTATG PSEAVWYGPP KAANLGGVAV SGLEMAQNSQ RITWTSERVD
410 420 430 440 450
QELKRIMINC FNECIDYAKK YTKDGKVLPS LVKGANIASF IKVSDAMFDQ

GDVF
Length:454
Mass (Da):49,570
Last modified:November 1, 1997 - v2
Checksum:i707F921B6FE5B5B1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83V → G in AAB03898 (PubMed:2989290).Curated1
Sequence conflicti198V → L in AAB03898 (PubMed:2989290).Curated1
Sequence conflicti255S → L in AAB03898 (PubMed:2989290).Curated1
Sequence conflicti266D → V in AAB03898 (PubMed:2989290).Curated1
Sequence conflicti358 – 364ATGPSEA → PLDQAT in AAA34642 (PubMed:2932370).Curated7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11297 Genomic DNA Translation: AAB03898.1
M10590 Genomic DNA Translation: AAA34642.1
Z75283 Genomic DNA Translation: CAA99706.1
BK006948 Genomic DNA Translation: DAA11134.1
PIRiS67287 A25275
RefSeqiNP_015020.3, NM_001183795.3

Genome annotation databases

EnsemblFungiiYOR375C; YOR375C; YOR375C
GeneIDi854557
KEGGisce:YOR375C

Similar proteinsi

Entry informationi

Entry nameiDHE4_YEAST
AccessioniPrimary (citable) accession number: P07262
Secondary accession number(s): D6W368, Q08899
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 177 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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