P07262 (DHE4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADP-specific glutamate dehydrogenase 1
Short name=NADP-GDH 1
EC=1.4.1.4

Alternative name(s):
NADP-dependent glutamate dehydrogenase 1

Gene names

Name:	GDH1
Synonyms:	URE1
Ordered Locus Names:	YOR375C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer.
Miscellaneous	Present with 77500 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ammonia assimilation cycle Inferred from expression pattern. Source: SGD glutamate biosynthetic process Inferred from genetic interaction Ref.2Ref.1. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD soluble fraction Inferred from direct assay. Source: SGD
Molecular function	glutamate dehydrogenase (NADP+) activity Inferred from direct assay Ref.1. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	NADP-specific glutamate dehydrogenase 1		PRO_0000182798
Regions
	Nucleotide binding	174 – 203	30	NAD By similarity
Sites
	Active site	110	1	By similarity
Amino acid modifications
	Cross-link	325		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.7
	Cross-link	371		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.7
Experimental info
	Sequence conflict	83	1	V → G in AAB03898. Ref.1
	Sequence conflict	198	1	V → L in AAB03898. Ref.1
	Sequence conflict	255	1	S → L in AAB03898. Ref.1
	Sequence conflict	266	1	D → V in AAB03898. Ref.1
	Sequence conflict	358 – 364	7	ATGPSEA → PLDQAT in AAA34642. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P07262 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 707F921B6FE5B5B1	FASTA	454	49,570
	10 20 30 40 50 60 MSEPEFQQAY EEVVSSLEDS TLFEQHPEYR KVLPIVSVPE RIIQFRVTWE NDKGEQEVAQ 70 80 90 100 110 120 GYRVQYNSAK GPYKGGLRFH PSVNLSILKF LGFEQIFKNS LTGLDMGGGK GGLCVDLKGR 130 140 150 160 170 180 SNNEIRRICY AFMRELSRHI GQDTDVPAGD IGVGGREIGY LFGAYRSYKN SWEGVLTGKG 190 200 210 220 230 240 LNWGGSLIRP EATGYGLVYY TQAMIDYATN GKESFEGKRV TISGSGNVAQ YAALKVIELG 250 260 270 280 290 300 GTVVSLSDSK GCIISETGIT SEQVADISSA KVNFKSLEQI VNEYSTFSEN KVQYIAGARP 310 320 330 340 350 360 WTHVQKVDIA LPCATQNEVS GEEAKALVAQ GVKFIAEGSN MGSTPEAIAV FETARSTATG 370 380 390 400 410 420 PSEAVWYGPP KAANLGGVAV SGLEMAQNSQ RITWTSERVD QELKRIMINC FNECIDYAKK 430 440 450 YTKDGKVLPS LVKGANIASF IKVSDAMFDQ GDVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of yeast GDH1 encoding nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase." Moye W.S., Amuro N., Rao J.K.M., Zalkin H. J. Biol. Chem. 260:8502-8508(1985) [PubMed: 2989290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of the GDH gene coding for the NADP-specific glutamate dehydrogenase of Saccharomyces cerevisiae." Nagasu T., Hall B.D. Gene 37:247-253(1985) [PubMed: 2932370] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae." Norbeck J., Blomberg A. FEMS Microbiol. Lett. 137:1-8(1996) [PubMed: 8935650] [Abstract] Cited for: PROTEIN SEQUENCE OF 79-88; 157-165 AND 236-246. Strain: ATCC 38531 / Y41.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-325 AND LYS-371, MASS SPECTROMETRY. Strain: SUB592.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M11297 Genomic DNA. Translation: AAB03898.1. M10590 Genomic DNA. Translation: AAA34642.1. Z75283 Genomic DNA. Translation: CAA99706.1. BK006948 Genomic DNA. Translation: DAA11134.1.
PIR	A25275. S67287.
RefSeq	NP_015020.1. NM_001183795.1.
3D structure databases
ProteinModelPortal	P07262.
SMR	P07262. Positions 2-453.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1329N.
IntAct	P07262. 36 interactions.
MINT	MINT-390545.
STRING	P07262.
Proteomic databases
PeptideAtlas	P07262.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR375C; YOR375C; YOR375C.
GeneID	854557.
KEGG	sce:YOR375C.
NMPDR	fig|4932.3.peg.6139.
Organism-specific databases
CYGD	YOR375c.
SGD	S000005902. GDH1.
Phylogenomic databases
eggNOG	fuNOG07140.
GeneTree	EFGT00050000006207.
HOGENOM	HBG590661.
OMA	FCVAFMR.
OrthoDB	EOG4PP1RB.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-11504.
Gene expression databases
ArrayExpress	P07262.
Genevestigator	P07262.
GermOnline	YOR375C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00262.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976988.

Entry information

Entry name

DHE4_YEAST

Accession

Primary (citable) accession number: P07262
Secondary accession number(s): D6W368, Q08899

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program