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P07262

- DHE4_YEAST

UniProt

P07262 - DHE4_YEAST

Protein

NADP-specific glutamate dehydrogenase 1

Gene

GDH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei110 – 1101PROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi174 – 20330NADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. glutamate dehydrogenase (NADP+) activity Source: SGD

    GO - Biological processi

    1. ammonia assimilation cycle Source: SGD
    2. glutamate biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-11504.
    YEAST:YOR375C-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADP-specific glutamate dehydrogenase 1 (EC:1.4.1.4)
    Short name:
    NADP-GDH 1
    Alternative name(s):
    NADP-dependent glutamate dehydrogenase 1
    Gene namesi
    Name:GDH1
    Synonyms:URE1
    Ordered Locus Names:YOR375C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR375c.
    SGDiS000005902. GDH1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 454453NADP-specific glutamate dehydrogenase 1PRO_0000182798Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Cross-linki325 – 325Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Cross-linki371 – 371Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

    Keywords - PTMi

    Acetylation, Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiP07262.
    PaxDbiP07262.
    PeptideAtlasiP07262.
    PRIDEiP07262.

    Expressioni

    Gene expression databases

    GenevestigatoriP07262.

    Interactioni

    Subunit structurei

    Homohexamer.

    Protein-protein interaction databases

    BioGridi34758. 47 interactions.
    DIPiDIP-1329N.
    IntActiP07262. 36 interactions.
    MINTiMINT-390545.
    STRINGi4932.YOR375C.

    Structurei

    3D structure databases

    ProteinModelPortaliP07262.
    SMRiP07262. Positions 3-451.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0334.
    GeneTreeiENSGT00390000000854.
    HOGENOMiHOG000243799.
    KOiK00262.
    OMAiSYGKMNS.
    OrthoDBiEOG7XSTPW.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000185. Glu_DH. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07262-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEPEFQQAY EEVVSSLEDS TLFEQHPEYR KVLPIVSVPE RIIQFRVTWE    50
    NDKGEQEVAQ GYRVQYNSAK GPYKGGLRFH PSVNLSILKF LGFEQIFKNS 100
    LTGLDMGGGK GGLCVDLKGR SNNEIRRICY AFMRELSRHI GQDTDVPAGD 150
    IGVGGREIGY LFGAYRSYKN SWEGVLTGKG LNWGGSLIRP EATGYGLVYY 200
    TQAMIDYATN GKESFEGKRV TISGSGNVAQ YAALKVIELG GTVVSLSDSK 250
    GCIISETGIT SEQVADISSA KVNFKSLEQI VNEYSTFSEN KVQYIAGARP 300
    WTHVQKVDIA LPCATQNEVS GEEAKALVAQ GVKFIAEGSN MGSTPEAIAV 350
    FETARSTATG PSEAVWYGPP KAANLGGVAV SGLEMAQNSQ RITWTSERVD 400
    QELKRIMINC FNECIDYAKK YTKDGKVLPS LVKGANIASF IKVSDAMFDQ 450
    GDVF 454
    Length:454
    Mass (Da):49,570
    Last modified:November 1, 1997 - v2
    Checksum:i707F921B6FE5B5B1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti83 – 831V → G in AAB03898. (PubMed:2989290)Curated
    Sequence conflicti198 – 1981V → L in AAB03898. (PubMed:2989290)Curated
    Sequence conflicti255 – 2551S → L in AAB03898. (PubMed:2989290)Curated
    Sequence conflicti266 – 2661D → V in AAB03898. (PubMed:2989290)Curated
    Sequence conflicti358 – 3647ATGPSEA → PLDQAT in AAA34642. (PubMed:2932370)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11297 Genomic DNA. Translation: AAB03898.1.
    M10590 Genomic DNA. Translation: AAA34642.1.
    Z75283 Genomic DNA. Translation: CAA99706.1.
    BK006948 Genomic DNA. Translation: DAA11134.1.
    PIRiS67287. A25275.
    RefSeqiNP_015020.3. NM_001183795.3.

    Genome annotation databases

    EnsemblFungiiYOR375C; YOR375C; YOR375C.
    GeneIDi854557.
    KEGGisce:YOR375C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11297 Genomic DNA. Translation: AAB03898.1 .
    M10590 Genomic DNA. Translation: AAA34642.1 .
    Z75283 Genomic DNA. Translation: CAA99706.1 .
    BK006948 Genomic DNA. Translation: DAA11134.1 .
    PIRi S67287. A25275.
    RefSeqi NP_015020.3. NM_001183795.3.

    3D structure databases

    ProteinModelPortali P07262.
    SMRi P07262. Positions 3-451.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34758. 47 interactions.
    DIPi DIP-1329N.
    IntActi P07262. 36 interactions.
    MINTi MINT-390545.
    STRINGi 4932.YOR375C.

    Proteomic databases

    MaxQBi P07262.
    PaxDbi P07262.
    PeptideAtlasi P07262.
    PRIDEi P07262.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR375C ; YOR375C ; YOR375C .
    GeneIDi 854557.
    KEGGi sce:YOR375C.

    Organism-specific databases

    CYGDi YOR375c.
    SGDi S000005902. GDH1.

    Phylogenomic databases

    eggNOGi COG0334.
    GeneTreei ENSGT00390000000854.
    HOGENOMi HOG000243799.
    KOi K00262.
    OMAi SYGKMNS.
    OrthoDBi EOG7XSTPW.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-11504.
    YEAST:YOR375C-MONOMER.

    Miscellaneous databases

    NextBioi 976988.

    Gene expression databases

    Genevestigatori P07262.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000185. Glu_DH. 1 hit.
    PRINTSi PR00082. GLFDHDRGNASE.
    SMARTi SM00839. ELFV_dehydrog. 1 hit.
    [Graphical view ]
    PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of yeast GDH1 encoding nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase."
      Moye W.S., Amuro N., Rao J.K.M., Zalkin H.
      J. Biol. Chem. 260:8502-8508(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Nucleotide sequence of the GDH gene coding for the NADP-specific glutamate dehydrogenase of Saccharomyces cerevisiae."
      Nagasu T., Hall B.D.
      Gene 37:247-253(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae."
      Norbeck J., Blomberg A.
      FEMS Microbiol. Lett. 137:1-8(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 79-88; 157-165 AND 236-246.
      Strain: ATCC 38531 / Y41.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-325 AND LYS-371.
      Strain: SUB592.
    8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDHE4_YEAST
    AccessioniPrimary (citable) accession number: P07262
    Secondary accession number(s): D6W368, Q08899
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 141 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 77500 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3