Eukaryotic translation initiation factor 4E - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P07260 (IF4E_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 127. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Eukaryotic translation initiation factor 4E
Short name=eIF-4E
Short name=eIF4E

Alternative name(s):
eIF-4F 25 kDa subunit
mRNA cap-binding protein

Gene names

Name:	CDC33
Synonyms:	TIF45
Ordered Locus Names:	YOL139C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Subunit structure	Component of the eIF4F complex, which composition varies with external and internal environmental conditions. It is composed of at least eIF4A (TIF1/TIF2), eIF4E (TIF45) and eIF4G (TIF4631 or TIF4632) By similarity. Interacts with PAT1 in a RNA-dependent manner. eIF4E is also known to interact with other partners. Ref.9
Miscellaneous	Present with 14200 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Belongs to the eukaryotic initiation factor 4E family.

Ontologies

Keywords
Biological process	Protein biosynthesis Translation regulation
Ligand	RNA-binding
Molecular function	Initiation factor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Inferred from direct assay. Source: SGD regulation of cell cycle Inferred from mutant phenotype. Source: SGD regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasmic stress granule Inferred from direct assay. Source: SGD eukaryotic translation initiation factor 4F complex Traceable author statement. Source: SGD nucleus Inferred from direct assay. Source: SGD ribosome Traceable author statement. Source: SGD
Molecular function	phosphatidylinositol-3-phosphate binding Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct translation initiation factor activity Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAF20	P12962	8	EBI-150,EBI-9010
TIF4631	P39935	10	EBI-150,EBI-9002

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 213

213

Eukaryotic translation initiation factor 4E

PRO_0000193653

Amino acid modifications

Modified residue

Phosphoserine; by CK2 Ref.8

Modified residue

Phosphoserine; by CK2 Ref.8 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15

Modified residue

Phosphothreonine Ref.12 Ref.14 Ref.15

Modified residue

Phosphoserine Ref.14 Ref.15

Modified residue

Phosphoserine Ref.12 Ref.14 Ref.15

Secondary structure

213

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P07260 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 5B9162E1BD0B1BDE
Show »

FASTA

213

24,254

        10         20         30         40         50         60 
MSVEEVSKKF EENVSVDDTT ATPKTVLSDS AHFDVKHPLN TKWTLWYTKP AVDKSESWSD 

        70         80         90        100        110        120 
LLRPVTSFQT VEEFWAIIQN IPEPHELPLK SDYHVFRNDV RPEWEDEANA KGGKWSFQLR 

       130        140        150        160        170        180 
GKGADIDELW LRTLLAVIGE TIDEDDSQIN GVVLSIRKGG NKFALWTKSE DKEPLLRIGG 

       190        200        210 
KFKQVLKLTD DGHLEFFPHS SANGRHPQPS ITL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"mRNA cap-binding protein: cloning of the gene encoding protein synthesis initiation factor eIF-4E from Saccharomyces cerevisiae." Altmann M., Handschin C., Trachsel H. Mol. Cell. Biol. 7:998-1003(1987) [PubMed: 3550438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"CDC33 encodes mRNA cap-binding protein eIF-4E of Saccharomyces cerevisiae." Brenner C., Nakayama N., Goebl M., Tanaka K., Toh-e A., Matsumoto K. Mol. Cell. Biol. 8:3556-3559(1988) [PubMed: 3062383] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Translation in Saccharomyces cerevisiae: initiation factor 4E-dependent cell-free system." Altmann M., Sonenberg N., Trachsel H. Mol. Cell. Biol. 9:4467-4472(1989) [PubMed: 2685552] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Sequence analysis of a 9873 bp fragment of the left arm of yeast chromosome XV that contains the ARG8 and CDC33 genes, a putative riboflavin synthase beta chain gene, and four new open reading frames." Casas C., Aldea M., Casamayor A., Lafuente M.J., Gamo F.J., Gancedo C., Arino J., Herrero E. Yeast 11:1061-1067(1995) [PubMed: 7502581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[6]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[7]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[8]	"Characterization of the in vivo phosphorylation sites of the mRNA.cap-binding complex proteins eukaryotic initiation factor-4E and p20 in Saccharomyces cerevisiae." Zanchin N.I.T., McCarthy J.E.G. J. Biol. Chem. 270:26505-26510(1995) [PubMed: 7592868] [Abstract] Cited for: PHOSPHORYLATION AT SER-2 AND SER-15.
[9]	"Targeting an mRNA for decapping: displacement of translation factors and association of the Lsm1p-7p complex on deadenylated yeast mRNAs." Tharun S., Parker R. Mol. Cell 8:1075-1083(2001) [PubMed: 11741542] [Abstract] Cited for: INTERACTION WITH PAT1.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15, MASS SPECTROMETRY. Strain: YAL6B.
[12]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15; THR-22 AND SER-30, MASS SPECTROMETRY. Strain: ADR376.
[13]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15, MASS SPECTROMETRY.
[14]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15; THR-22; SER-28 AND SER-30, MASS SPECTROMETRY.
[15]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15; THR-22; SER-28 AND SER-30, MASS SPECTROMETRY.
[16]	"Structure of translation factor eIF4E bound to m7GDP and interaction with 4E-binding protein." Matsuo H., Li H., McGuire A.M., Fletcher C.M., Gingras A.-C., Sonenberg N., Wagner G. Nat. Struct. Biol. 4:717-724(1997) [PubMed: 9302999] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M15436 Genomic DNA. Translation: AAA34587.1.
M21620 Genomic DNA. Translation: AAA34480.1.
M29251 Genomic DNA. Translation: AAA34588.1.
X84036 Genomic DNA. Translation: CAA58854.1.
Z74881 Genomic DNA. Translation: CAA99160.1.
AY692936 Genomic DNA. Translation: AAT92955.1.
BK006948 Genomic DNA. Translation: DAA10646.1.

PIR

A26130.

RefSeq

NP_014502.1. NM_001183393.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AP8	NMR	-	A	1-213	[»]
1RF8	NMR	-	A	1-213	[»]

ProteinModelPortal

P07260.

SMR

P07260. Positions 1-213.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1223N.

IntAct

P07260. 73 interactions.

MINT

MINT-403379.

STRING

P07260.

Proteomic databases

PeptideAtlas

P07260.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YOL139C; YOL139C; YOL139C.

GeneID

854026.

KEGG

sce:YOL139C.

NMPDR

fig|4932.3.peg.5588.

Organism-specific databases

CYGD

YOL139c.

SGD

S000005499. CDC33.

Phylogenomic databases

eggNOG

fuNOG08256.

GeneTree

EFGT00050000004603.

HOGENOM

HBG559144.

OMA

HEWTLWF.

OrthoDB

EOG41CB5P.

Gene expression databases

ArrayExpress

P07260.

Genevestigator

P07260.

GermOnline

YOL139C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR023398. TIF_eIF4e-like_dom.
IPR001040. TIF_eIF_4E.
IPR019770. TIF_eIF_4E_CS.
[Graphical view]

Gene3D

G3DSA:3.30.760.10. TIF_eIF_4E. 1 hit.

K03259.

PANTHER

PTHR11960. TIF_eIF_4E. 1 hit.

Pfam

PF01652. IF4E. 1 hit.
[Graphical view]

SUPFAM

SSF55418. TIF_eIF_4E. 1 hit.

PROSITE

PS00813. IF4E. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

975568.

Entry information

Entry name

IF4E_YEAST

Accession

Primary (citable) accession number: P07260
Secondary accession number(s): D6W1T0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	December 14, 2011
This is version 127 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Translation initiation factors

List of translation initiation factor entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents