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Protein

Eukaryotic translation initiation factor 4E

Gene

CDC33

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

GO - Molecular functioni

  • phosphatidylinositol-3-phosphate binding Source: SGD
  • translation initiation factor activity Source: SGD

GO - Biological processi

  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: SGD
  • regulation of cell cycle Source: SGD
  • regulation of translation Source: UniProtKB-KW
  • translational initiation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33532-MONOMER.
ReactomeiR-SCE-1169408. ISG15 antiviral mechanism.
R-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-SCE-72702. Ribosomal scanning and start codon recognition.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4E
Short name:
eIF-4E
Short name:
eIF4E
Alternative name(s):
eIF-4F 25 kDa subunit
mRNA cap-binding protein
Gene namesi
Name:CDC33
Synonyms:TIF45
Ordered Locus Names:YOL139C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL139C.
SGDiS000005499. CDC33.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytoplasmic stress granule Source: SGD
  • eukaryotic translation initiation factor 4F complex Source: SGD
  • nucleus Source: SGD
  • ribosome Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001936531 – 213Eukaryotic translation initiation factor 4EAdd BLAST213

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2Phosphoserine; by CK21 Publication1
Modified residuei15Phosphoserine; by CK2Combined sources1 Publication1
Modified residuei22PhosphothreonineCombined sources1
Modified residuei28PhosphoserineCombined sources1
Modified residuei30PhosphoserineCombined sources1
Cross-linki114Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP07260.
PRIDEiP07260.
TopDownProteomicsiP07260.

PTM databases

iPTMnetiP07260.

Interactioni

Subunit structurei

Component of the eIF4F complex, which composition varies with external and internal environmental conditions. It is composed of at least eIF4A (TIF1/TIF2), eIF4E (TIF45) and eIF4G (TIF4631 or TIF4632) (By similarity). Interacts with PAT1 in a RNA-dependent manner. eIF4E is also known to interact with other partners.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CAF20P129626EBI-150,EBI-9010
TIF4631P3993515EBI-150,EBI-9002

Protein-protein interaction databases

BioGridi34278. 124 interactors.
DIPiDIP-1223N.
IntActiP07260. 48 interactors.
MINTiMINT-403379.

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 12Combined sources4
Beta strandi15 – 17Combined sources3
Helixi26 – 29Combined sources4
Beta strandi36 – 48Combined sources13
Helixi52 – 54Combined sources3
Helixi58 – 61Combined sources4
Beta strandi63 – 71Combined sources9
Helixi72 – 79Combined sources8
Turni83 – 86Combined sources4
Beta strandi92 – 96Combined sources5
Turni104 – 111Combined sources8
Beta strandi112 – 119Combined sources8
Beta strandi124 – 126Combined sources3
Helixi127 – 137Combined sources11
Turni140 – 142Combined sources3
Turni146 – 148Combined sources3
Beta strandi149 – 155Combined sources7
Beta strandi161 – 170Combined sources10
Helixi172 – 185Combined sources14
Beta strandi189 – 191Combined sources3
Beta strandi195 – 197Combined sources3
Helixi199 – 203Combined sources5
Beta strandi210 – 212Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AP8NMR-A1-213[»]
1RF8NMR-A1-213[»]
ProteinModelPortaliP07260.
SMRiP07260.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07260.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00520000055549.
HOGENOMiHOG000186751.
InParanoidiP07260.
KOiK03259.
OMAiVYNNIAP.
OrthoDBiEOG092C4INY.

Family and domain databases

Gene3Di3.30.760.10. 1 hit.
InterProiIPR023398. TIF_eIF4e-like.
IPR001040. TIF_eIF_4E.
IPR019770. TIF_eIF_4E_CS.
[Graphical view]
PANTHERiPTHR11960. PTHR11960. 1 hit.
PfamiPF01652. IF4E. 1 hit.
[Graphical view]
SUPFAMiSSF55418. SSF55418. 1 hit.
PROSITEiPS00813. IF4E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07260-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVEEVSKKF EENVSVDDTT ATPKTVLSDS AHFDVKHPLN TKWTLWYTKP
60 70 80 90 100
AVDKSESWSD LLRPVTSFQT VEEFWAIIQN IPEPHELPLK SDYHVFRNDV
110 120 130 140 150
RPEWEDEANA KGGKWSFQLR GKGADIDELW LRTLLAVIGE TIDEDDSQIN
160 170 180 190 200
GVVLSIRKGG NKFALWTKSE DKEPLLRIGG KFKQVLKLTD DGHLEFFPHS
210
SANGRHPQPS ITL
Length:213
Mass (Da):24,254
Last modified:April 1, 1988 - v1
Checksum:i5B9162E1BD0B1BDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15436 Genomic DNA. Translation: AAA34587.1.
M21620 Genomic DNA. Translation: AAA34480.1.
M29251 Genomic DNA. Translation: AAA34588.1.
X84036 Genomic DNA. Translation: CAA58854.1.
Z74881 Genomic DNA. Translation: CAA99160.1.
AY692936 Genomic DNA. Translation: AAT92955.1.
BK006948 Genomic DNA. Translation: DAA10646.1.
PIRiA26130.
RefSeqiNP_014502.1. NM_001183393.1.

Genome annotation databases

EnsemblFungiiYOL139C; YOL139C; YOL139C.
GeneIDi854026.
KEGGisce:YOL139C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15436 Genomic DNA. Translation: AAA34587.1.
M21620 Genomic DNA. Translation: AAA34480.1.
M29251 Genomic DNA. Translation: AAA34588.1.
X84036 Genomic DNA. Translation: CAA58854.1.
Z74881 Genomic DNA. Translation: CAA99160.1.
AY692936 Genomic DNA. Translation: AAT92955.1.
BK006948 Genomic DNA. Translation: DAA10646.1.
PIRiA26130.
RefSeqiNP_014502.1. NM_001183393.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AP8NMR-A1-213[»]
1RF8NMR-A1-213[»]
ProteinModelPortaliP07260.
SMRiP07260.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34278. 124 interactors.
DIPiDIP-1223N.
IntActiP07260. 48 interactors.
MINTiMINT-403379.

PTM databases

iPTMnetiP07260.

Proteomic databases

MaxQBiP07260.
PRIDEiP07260.
TopDownProteomicsiP07260.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL139C; YOL139C; YOL139C.
GeneIDi854026.
KEGGisce:YOL139C.

Organism-specific databases

EuPathDBiFungiDB:YOL139C.
SGDiS000005499. CDC33.

Phylogenomic databases

GeneTreeiENSGT00520000055549.
HOGENOMiHOG000186751.
InParanoidiP07260.
KOiK03259.
OMAiVYNNIAP.
OrthoDBiEOG092C4INY.

Enzyme and pathway databases

BioCyciYEAST:G3O-33532-MONOMER.
ReactomeiR-SCE-1169408. ISG15 antiviral mechanism.
R-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-SCE-72702. Ribosomal scanning and start codon recognition.

Miscellaneous databases

EvolutionaryTraceiP07260.
PROiP07260.

Family and domain databases

Gene3Di3.30.760.10. 1 hit.
InterProiIPR023398. TIF_eIF4e-like.
IPR001040. TIF_eIF_4E.
IPR019770. TIF_eIF_4E_CS.
[Graphical view]
PANTHERiPTHR11960. PTHR11960. 1 hit.
PfamiPF01652. IF4E. 1 hit.
[Graphical view]
SUPFAMiSSF55418. SSF55418. 1 hit.
PROSITEiPS00813. IF4E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF4E_YEAST
AccessioniPrimary (citable) accession number: P07260
Secondary accession number(s): D6W1T0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 2, 2016
This is version 172 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 14200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.