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Protein

Carbamoyl-phosphate synthase arginine-specific small chain

Gene

CPA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase arginine-specific large chain (CPA2), Carbamoyl-phosphate synthase arginine-specific small chain (CPA1)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei264NucleophileBy similarity1
Active sitei349By similarity1
Active sitei351By similarity1

GO - Molecular functioni

GO - Biological processi

  • 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
  • arginine biosynthetic process Source: SGD
  • glutamine metabolic process Source: InterPro
  • urea cycle Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YOR303W-MONOMER.
UniPathwayiUPA00068; UER00171.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase arginine-specific small chain (EC:6.3.5.5)
Short name:
CPS-A
Alternative name(s):
Arginine-specific carbamoyl-phosphate synthetase, glutamine chain
Gene namesi
Name:CPA1
Ordered Locus Names:YOR303W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR303W.
SGDiS000005829. CPA1.

Subcellular locationi

GO - Cellular componenti

  • carbamoyl-phosphate synthase complex Source: SGD
  • cytoplasmic stress granule Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001123721 – 411Carbamoyl-phosphate synthase arginine-specific small chainAdd BLAST411

Proteomic databases

MaxQBiP07258.
PRIDEiP07258.

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.

Protein-protein interaction databases

BioGridi34691. 25 interactors.
DIPiDIP-1024N.
IntActiP07258. 4 interactors.
MINTiMINT-412445.

Structurei

3D structure databases

ProteinModelPortaliP07258.
SMRiP07258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini185 – 376Glutamine amidotransferase type-1Add BLAST192

Sequence similaritiesi

Belongs to the CarA family.Curated

Phylogenomic databases

GeneTreeiENSGT00860000135115.
HOGENOMiHOG000038087.
InParanoidiP07258.
KOiK01956.
OMAiCFSVQYH.
OrthoDBiEOG092C20Y3.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view]
PfamiPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTiSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07258-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSAATKATF CIQNGPSFEG ISFGANKSVA GETVFTTSLV GYPESMTDPS
60 70 80 90 100
YRGQILVFTQ PLIGNYGVPS GEARDEYNLL KYFESPHIHV VGIVVAEYAY
110 120 130 140 150
QYSHWTAVES LAQWCQREGV AAITGVDTRE LVQYLREQGS SLGRITLADH
160 170 180 190 200
DPVPYVNPMK TNLVAQVTTK KPFHVSALPG KAKANVALID CGVKENIIRC
210 220 230 240 250
LVKRGANVTV FPYDYRIQDV ASEFDGIFLS NGPGNPELCQ ATISNVRELL
260 270 280 290 300
NNPVYDCIPI FGICLGHQLL ALASGASTHK LKYGNRAHNI PAMDLTTGQC
310 320 330 340 350
HITSQNHGYA VDPETLPKDQ WKPYFVNLND KSNEGMIHLQ RPIFSTQFHP
360 370 380 390 400
EAKGGPLDTA ILFDKFFDNI EKYQLQSQAK SSISLKVTYS TDKSRLQSIN
410
VTKLAKERVL F
Length:411
Mass (Da):45,362
Last modified:April 1, 1988 - v1
Checksum:i0793442274770614
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01764 Genomic DNA. Translation: CAA25905.1.
K02132 Genomic DNA. Translation: AAA34525.1.
Z75211 Genomic DNA. Translation: CAA99621.1.
M16690 Genomic DNA. Translation: AAA34527.1.
BK006948 Genomic DNA. Translation: DAA11068.1.
PIRiS67207. SYBYCS.
RefSeqiNP_014947.3. NM_001183722.3.

Genome annotation databases

EnsemblFungiiYOR303W; YOR303W; YOR303W.
GeneIDi854479.
KEGGisce:YOR303W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01764 Genomic DNA. Translation: CAA25905.1.
K02132 Genomic DNA. Translation: AAA34525.1.
Z75211 Genomic DNA. Translation: CAA99621.1.
M16690 Genomic DNA. Translation: AAA34527.1.
BK006948 Genomic DNA. Translation: DAA11068.1.
PIRiS67207. SYBYCS.
RefSeqiNP_014947.3. NM_001183722.3.

3D structure databases

ProteinModelPortaliP07258.
SMRiP07258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34691. 25 interactors.
DIPiDIP-1024N.
IntActiP07258. 4 interactors.
MINTiMINT-412445.

Proteomic databases

MaxQBiP07258.
PRIDEiP07258.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR303W; YOR303W; YOR303W.
GeneIDi854479.
KEGGisce:YOR303W.

Organism-specific databases

EuPathDBiFungiDB:YOR303W.
SGDiS000005829. CPA1.

Phylogenomic databases

GeneTreeiENSGT00860000135115.
HOGENOMiHOG000038087.
InParanoidiP07258.
KOiK01956.
OMAiCFSVQYH.
OrthoDBiEOG092C20Y3.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
BioCyciYEAST:YOR303W-MONOMER.

Miscellaneous databases

PROiP07258.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view]
PfamiPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTiSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCARA_YEAST
AccessioniPrimary (citable) accession number: P07258
Secondary accession number(s): D6W302
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 30, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes this enzyme is synthesized by two pathway-specific (arginine and pyrimidine) under separate control.
Present with 2580 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.