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Protein

Carbamoyl-phosphate synthase arginine-specific small chain

Gene

CPA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

In eukaryotes this enzyme is synthesized by two pathway-specific (arginine and pyrimidine) under separate control.
Present with 2580 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase arginine-specific large chain (CPA2), Carbamoyl-phosphate synthase arginine-specific small chain (CPA1)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei264NucleophileBy similarity1
Active sitei349By similarity1
Active sitei351By similarity1

GO - Molecular functioni

GO - Biological processi

  • 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
  • arginine biosynthetic process Source: SGD
  • glutamine metabolic process Source: InterPro
  • urea cycle Source: GO_Central

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YOR303W-MONOMER.
UniPathwayiUPA00068; UER00171.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase arginine-specific small chain (EC:6.3.5.5)
Short name:
CPS-A
Alternative name(s):
Arginine-specific carbamoyl-phosphate synthetase, glutamine chain
Gene namesi
Name:CPA1
Ordered Locus Names:YOR303W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR303W.
SGDiS000005829. CPA1.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001123721 – 411Carbamoyl-phosphate synthase arginine-specific small chainAdd BLAST411

Proteomic databases

MaxQBiP07258.
PRIDEiP07258.

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.

Protein-protein interaction databases

BioGridi34691. 25 interactors.
DIPiDIP-1024N.
IntActiP07258. 5 interactors.
MINTiMINT-412445.
STRINGi4932.YOR303W.

Structurei

3D structure databases

ProteinModelPortaliP07258.
SMRiP07258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini185 – 376Glutamine amidotransferase type-1Add BLAST192

Sequence similaritiesi

Belongs to the CarA family.Curated

Phylogenomic databases

GeneTreeiENSGT00900000142653.
HOGENOMiHOG000038087.
InParanoidiP07258.
KOiK01956.
OMAiCFNTGMT.
OrthoDBiEOG092C20Y3.

Family and domain databases

CDDicd01744. GATase1_CPSase. 1 hit.
Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiView protein in InterPro
IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR036480. CarbP_synth_ssu_N_sf.
IPR029062. Class_I_gatase-like.
IPR035686. CPSase_GATase1.
IPR017926. GATASE.
PfamiView protein in Pfam
PF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
SMARTiView protein in SMART
SM01097. CPSase_sm_chain. 1 hit.
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiView protein in PROSITE
PS51273. GATASE_TYPE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

P07258-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSAATKATF CIQNGPSFEG ISFGANKSVA GETVFTTSLV GYPESMTDPS
60 70 80 90 100
YRGQILVFTQ PLIGNYGVPS GEARDEYNLL KYFESPHIHV VGIVVAEYAY
110 120 130 140 150
QYSHWTAVES LAQWCQREGV AAITGVDTRE LVQYLREQGS SLGRITLADH
160 170 180 190 200
DPVPYVNPMK TNLVAQVTTK KPFHVSALPG KAKANVALID CGVKENIIRC
210 220 230 240 250
LVKRGANVTV FPYDYRIQDV ASEFDGIFLS NGPGNPELCQ ATISNVRELL
260 270 280 290 300
NNPVYDCIPI FGICLGHQLL ALASGASTHK LKYGNRAHNI PAMDLTTGQC
310 320 330 340 350
HITSQNHGYA VDPETLPKDQ WKPYFVNLND KSNEGMIHLQ RPIFSTQFHP
360 370 380 390 400
EAKGGPLDTA ILFDKFFDNI EKYQLQSQAK SSISLKVTYS TDKSRLQSIN
410
VTKLAKERVL F
Length:411
Mass (Da):45,362
Last modified:April 1, 1988 - v1
Checksum:i0793442274770614
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01764 Genomic DNA. Translation: CAA25905.1.
K02132 Genomic DNA. Translation: AAA34525.1.
Z75211 Genomic DNA. Translation: CAA99621.1.
M16690 Genomic DNA. Translation: AAA34527.1.
BK006948 Genomic DNA. Translation: DAA11068.1.
PIRiS67207. SYBYCS.
RefSeqiNP_014947.3. NM_001183722.3.

Genome annotation databases

EnsemblFungiiYOR303W; YOR303W; YOR303W.
GeneIDi854479.
KEGGisce:YOR303W.

Similar proteinsi

Entry informationi

Entry nameiCARA_YEAST
AccessioniPrimary (citable) accession number: P07258
Secondary accession number(s): D6W302
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: October 25, 2017
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names